63 results about "Sedum plumbizincicola" patented technology

The invention relates to a remediation method of heavy metal contaminated soil, which comprises the following steps that: the heavy metal contaminated soil with lower soil nutrient and organic matter contents is selected to be remedied; the remediation plant sedum plumbizincicola is planted; an organic waste and a bio-surfactant are respectively added at a rhizosphere when the plant height grow to be 5 to 10cm; the bio-surfactant is repeatedly added to the soil every 60d; and after the remediation plant matures, the ground and underground parts are harvested and are centrally treated; and the content of heavy metal in soil is determined. By adding agricultural organic wastes, the soil nutrient and organic matter contents are improved, the increase of plant growth and root exudates is promoted, thus increasing the accumulation of heavy metal in underground roots and ground stems and leaves of the plants, and the quantity of rhizosphere soil microbes. The heavy metals are desorbed from soil particles by the bio-surfactant so as to improve the bioavailability of the heavy metals.

The invention discloses a method of quickly repairing a cadmium polluted cultivated land by crop rotation of a cadmium super-enriching plant and an energy plant. The method comprises the following step: planting the cadmium super-enriching plant and the energy plant in the cadmium polluted cultivated land in the natural growth periods thereof for conventional cultivation to achieve the purpose ofrepairing the cadmium polluted cultivated land by means of adsorbing and enriching abilities of the cadmium super-enriching plant and the energy plant, wherein the cadmium super-enriching plant is sedum plumbizincicola and the energy plant is sorghum or oilseed rape. The invention provides a planting mode which is not limited by growth seasons. Based on one year as the repair period, the soil repair efficiency of the polluted cultivated land is increased by means of a relatively long growth period in multi-cropping plantation, and the method is short in repair period, high in operability and low in cost, and repairs the land more thoroughly.

The invention provides the application of trichoderma reesei and sedum plumbizincicola in the remediation of cadmium-polluted farmland soil. The application comprises the following steps of: cutting sedum plumbizincicola seedlings with about 10cm of plant height into soil to be remedied, applying trichoderma reesei FS10-C fermentation liquor or microbial preparation to the rhizosphere of the plant, keeping the soil water content to be 70%wt of the maximum field capacity in the process of remediation, and scissoring the plant along the surface of soil after the plant grows for 60-150 days to complete one remediation process. According to the application, the plant remediation efficiency of the cadmium-polluted farmland soil can be improved; and the sedum plumbizincicola plant remediation efficiency can be improved according to the trichoderma preparation, the biomass of the soil microorganism can be enhanced, the micro-ecological environment of the soil can be improved, and the application is good in ecological efficiency, and suitable for the remediation of the cadmium-polluted farmland soil.

The invention discloses a method for remedying heavy metal cadmium contamination in soil. The method comprises the following steps: planting sedum plumbizincicola and helianthus annuus in cadmium-contaminated soil in an intercropping form; absorbing and enriching heavy metal cadmium by the sedum plumbizincicola and the helianthus annuus to remove cadmium from the soil. The intercropping helianthusannuus provides a good shading effect for growth of the sedum plumbizincicola, so that the input cost is reduced and the problem of safe survival of the sedum plumbizincicola in summer is effectivelyalleviated; a technical model is easy to operate, economical and practical, and is easily accepted by agricultural producers.

The invention discloses a regulation and control method for cadmium contaminated farmland soil to produce while repairing. The method includes performing air-drying and sieving on cadmium contaminatedsoil, applying a regulator component A, B and C, applying urea, and performing uniform mixing; planting paddy rice, performing drying for 3 days except for the later period of a tillering stage, maintaining a water layer of 2-3 cm at other time, performing frying until 3 days before harvest, and harvesting the paddy rice; performing re-air drying and sieving on the soil, applying a regulator component D and E, applying the urea, and performing uniform mixing; planting sedum plumbizincicola, maintaining the moisture of the soil at 60%-70% of maximum field water holding capacity, and harvestingthe upper parts of the sedum plumbizincicola after 4-6 months of planting; and repeating the above steps for 2-5 times so that repairing can be completed. The dual-purpose of guaranteeing the safe production of the paddy rice and accelerating repairing process can be achieved. The method can simultaneously consider the standard achieved production of the paddy rice on the contaminated soil and the efficient repairing of the sedum plumbizincicola.

The invention relates to a cadmium-contaminated soil remediation method. According to the cadmium-contaminated soil remediation method, a cadmium hyperaccumulator, namely sedum plumbizincicola, is planted in soil contaminated by heavy metal cadmium through a ridge-planting cuttage method, and an annual medicinal herbaceous cadmium hyperaccumulator, namely sticktight, is planted around the sedum plumbizincicola through intercropping; and the two hyperaccumulators are reasonably inter-planted to form upper and lower inter-planting structures of two crops by fully using the physiological differences of the two hyperaccumulators in height, light richness, shade tolerance, root depth and the like as well as the differences in growth season and space in the growing process, a good shading effectis achieved for the sedum plumbizincicola, and a combined remediation effect is achieved. By adoption of the cadmium-contaminated soil remediation method, the sedum plumbizincicola can safely grow insummer, the remediation effect is improved, and certain economic value is achieved. The cadmium-contaminated soil remediation method is high in space utilization rate, reliable in remediation effect,easy and convenient to operate, economical, environmentally friendly, good in application prospect and particularly suitable for remediating farmland soil contaminated by heavy metal cadmium to different degrees.

The invention discloses a method for remediating lead-cadmium combined polluted soil. The method is characterized in that sodium chloride is applied to the lead-cadmium polluted soil, and then sedum plumbizincicola is planted to extract cadmium from the soil, so as to achieve the purpose of reducing the content of chloride in the soil; after the sedum plumbizincicola is harvested, water soluble phosphate is applied to the soil, so as to reduce the effectiveness of lead in the soil. The method has the advantages that the remediation is simple and easy to master; the chloride is extracted through plant, and lead is stabilized through phosphate, so that the remediation effect is far more than that of an existing method; chlorine applied to the lead-cadmium combined polluted soil can improve the extraction of the sedum plumbizincicola to cadmium in the soil, and the stability of lead in the soil can be improved through phosphate.

The invention relates to a method suitable for large-area planting of sedum plumbizincicola. The method comprises the following steps: rotary tillage is carried out before transplanting next year, field parcel ridging is carried out in late February, and field parcels are covered with mulch films after herbicide spraying operation; in early March, transplanting of the sedum plumbizincicola is carried out, and perforating and transplanting operation is carried out on the mulch films; in middle July, the sedum plumbizincicola is cut while stubbles that are 3 to 5 cm in length are left uncut, andthe stubbles can grow again; the cut sedum plumbizincicola is cut into 8~10 cm single-branched cuttings which are then immersed in an acetylsalicylic acid solution before subjected being cottage operation, a sunshade was set up above a planting area and harvest can be reaped in middle November. Via adoption of the planting method disclosed in the invention, survival rates of the sedum plumbizincicola can reach above 90%, a two season propagation coefficient can reach above 40 times, and weed removal rates are controlled to be more than 60%. The method is characterized by simple operation, small investment, good effects and the like; a problem of large area weed control in sedum plumbizincicola plantation can be solved, costs can be greatly lowered, and survival rates and the biomass of the sedum plumbizincicola can be remarkably increased.

The invention relates to the technical field of phytoremediation of heavy metal compound contaminated soil, and discloses a method for remediating lead-cadmium compound contaminated soil by cooperating corn straw with sedum plumbizincicola intercropping energy plants, wherein the method comprises the following steps: corn straw is added into the lead-cadmium compound contaminated soil to obtain amatrix; sedum plumbizincicola and ricinus communis are intercropped in the middle of the matrix, and the application amount of the corn straw is 1%-2% of the mass of the lead-cadmium composite contaminated soil. According to the method, a method of utilizing the corn straw to cooperate with the sedum plumbizincicola intercropped with the ricinus communis to repair the lead-cadmium compound contaminated soil is a feasible repair mode; the content of pollutants in the lead-cadmium compound contaminated soil can be stably reduced, the yield of energy plants can be increased to a certain extent, it is guaranteed that farmers have certain economic benefits, and the actual requirement for 'simultaneous production and remediation' can be met.

The invention relates to a green biodegradable soil heavy metal cadmium activating agent. The green biodegradable soil heavy metal cadmium activating agent comprises, by weight, 3.5-31.5 parts of tetrasodium glutamate diacetate, 19.2-21.2 parts of citric acid, 7.5%-37.3 parts of potassium chloride, 10-18 parts of alpha-(2-methyl-1-oxo-2-propenyl)-omega-hydroxy-poly(oxy-2-ethanediyl), 5-9 parts ofa polyoxyethylene-polyoxypropylene block copolymer, 12-22 parts of sodium alga acid and 3-5 parts of calcium chloride. The green biodegradable soil heavy metal cadmium activating agent can promote sedum plumbizincicola to absorb heavy metal cadmium within the growth cycle of the sedum plumbizincicola, and the absorption rate of the heavy metal cadmium can be increased.

The invention discloses an ecological restoration method of zinc-cadmium heavy metal contaminated soil. The mode of moso bamboo and sedum plumbizincicola intercropping is adopted, the root growth layers of the two plants are crossed, thus the obvious nutrient competition relation does not exist, the root depths of the two plants are different, and thus the characteristics of fertilizers needed bythe two plants are also different. The root of the sedum plumbizincicola is generally located on the soil surface layer, when the depth of the contaminated soil reaches 30-40 cm, zinc-cadmium heavy metal in the soil cannot be absorbed by the root of the sedum plumbizincicola, but the depth of the root of the moso bamboo can reach 30-40 cm and conduct heavy metal ecological restoration on the soilbelow the surface layer of the contaminated soil.

The invention provides a substrate suitable for simple direct seeding seedling cultivation of sedum plumbizincicola and a direct seeding seedling cultivation method, wherein a formula of the substratecomprises cow dung type earthworm manure, sawdust and turf in a volume ratio of 3: 4: 2; and the direct seeding seedling cultivation method comprises the following steps of: treating stem segments ofthe sedum plumbizincicola by using a medicament, throwing and directly seeding the stem segments on a seedling cultivation substrate, then spraying the medicament to promote rapid rooting and inducegeneration of secondary buds, and pruning a large amount of the secondary buds to carry out the simple direct seeding seedling cultivation. The substrate suitable for the simple direct seeding seedling cultivation of the sedum plumbizincicola and the direct seeding seedling cultivation method have the advantages that sawmill waste is utilized, and the sawmill waste has the advantages of low price,light texture and high porosity, can effectively increase air permeability of the substrate, reduce density of the substrate, and is beneficial to germination and growth of a root system of direct seeding stem sections; and meanwhile, benign biomass energy circulation is achieved, and cost of the substrate for the sedum plumbizincicola seedling cultivation is reduced. By the adoption of the substrate suitable for the simple direct seeding seedling cultivation of the sedum plumbizincicola and the direct seeding seedling cultivation method, a throwing direct seeding seedling cultivation mode iscombined with a special seedling cultivation substrate, so that cost of the sedum plumbizincicola seedling cultivation is effectively reduced, seedling cultivation efficiency is improved, and a powerful guarantee is provided for an environmental restoration application of the sedum plumbizincicola.

The invention discloses a method for restoring heavy metal cadmium-polluted soil by using sedum plumbizincicola-fructus trichosanthis. According to the invention, cadmium hyperaccumulation plant sedumplumbizincicola and climbing-type herbaceous plant fructus trichosanthis are planted in the cadmium-polluted soil in an interplanting form; fructus trichosanthis is intensively grown above the sedumplumbizincicola, and which provides good shading environment for sedum plumbizincicola. The interplanted fructus trichosanthis increases the survival rate of sedum plumbizincicola in summer, and provides certain economic value, and the method is a technical mode having high space utilization rate and good restoration effect and is easily accepted by agriculture producers.

The invention relates to the technical field of phytoremediation of contaminated soil, in particular to a method for removing heavy metal organisms from soil. The method comprises the following steps: in November of the first year, ridging on a ploughed plot, planting ryegrass on the bottoms and slopes of ridges, and cutting and transplanting sedum plumbizincicola on the tops of the ridges; harvesting a first crop of the ryegrass from late March to April in the second year, and removing the whole plants of the ryegrass on the bottoms and slopes of the ridges in the middle of May; transplanting pennisetum sinese seedlings on the tops of the ridges in the middle of March in the second year, and removing the whole plants of the sedum plumbizincicola from April to May; harvesting a first crop of the pennisetum sinese in July and remaining stubble for 5 cm or above, and harvesting the whole plants of the pennisetum sinese in November. According to the method, a fertility cycle is reasonably and efficiently utilized, and harvesting crops for rotation are arranged, so that plant biomass is maximized and the absorptive amount of heavy metal in the soil is maximized, and the land utilization rate is improved.

The invention provides a plant mixed cultivation remediation method for phthalate-polycyclic aromatic hydrocarbon-heavy metal polluted soil, and belongs to the technical field of soil remediation. Theplant mixed cultivation remediation method for the phthalate-polycyclic aromatic hydrocarbon-heavy metal polluted soil comprises the step of planting alfalfa, elsholtzia splendens and sedum plumbizincicola on contaminated soil through mixed cultivation. The plant mixed cultivation remediation method for the phthalate-polycyclic aromatic hydrocarbon-heavy metal polluted soil, provided by the invention, is simple and convenient to operate, can treat various pollutants in the soil by using the plants planted through mixed cultivation, and has the advantages of ecological environmental protectionand rapid soil remediation.

A rapid cutting propagation method for zinc and cadmium hyperaccumulator sedum plumbizincicola comprises the following steps: stems of the sedum plumbizincicola are cut out, bases are inserted in a rooting culture solution and cultured at 15-23 DEG C for 7-20 days, the cultured stems are transplanted in a seedling culture substrate and managed by a traditional farming method, overground parts areharvested after plants grow to 15 cm-30 cm, the overground parts as seedlings are cut into stems of 6-10 cm with axillary buds again, and the stems with the axillary buds are cut in a seedling culturesubstrate or a nutrient solution, wherein the rooting culture solution is a base nutrient solution containing an auxin analogue and a cytokinin analogue in a concentration ratio of 1:2, the base nutrient solution is a 50% Japanese gardening formula nutrient solution, and the seedling culture substrate contains vermiculite and perlite in a ratio of 3:1 or an organic soil substrate. The cutting propagation method for the sedum plumbizincicola greatly improves the cutting propagation rate, the rooting speed and the survival rate of the sedum plumbizincicola, solves the key technical problem of rapid propagation of the sedum plumbizincicola, and has important values for rapid propagation and industrial seedling culture production and promotion of the sedum plumbizincicola.

The invention discloses creation of heavy metal super-enriched genetically-engineered rape genetically modified with a Sedum plumbizincicola SpNramp5 gene. The creation comprises steps as follows: theSpNramp5 gene in Sedum plumbizincicola is cloned; based on a plant expression vector, a transformation vector containing a strong promoter and a screening gene is established, the SpNramp5 gene is inserted into the transformation vector with a homologous recombination method, and an overexpression binary vector is obtained; the overexpression binary vector is transformed into a hypocotyl segmentof the rape by agrobacterium tumefaciens, genetically-modified seedlings are cultured, and genetically-modified positive plants are screened, wherein the transgenic receptor plants are cabbage type rape K407. According to the culture method, the problems of limited endurance, slow growth, small biomass, special growing environment, heavy metal return and the like of a wild heavy metal enriched plant can be solved. Besides, the invention further provides an application of the heavy metal super-enriched genetically-modified plant obtained through the creation of the heavy metal super-enriched genetically-engineered rape genetically modified with the Sedum plumbizincicola SpNramp5 gene to restoration of heavy metal contaminated soil.

The invention provides a sedum plumbizincicola multi-harvest and cadmium-polluted soil remediation method. According to the method, sedum plumbizincicola and cynodon dactylon are interplanted. The sedum plumbizincicola and the cynodon dactylon are reasonably interplanted, efficient remediation and reasonable cutting measures are taken, the technical effect that the sedum plumbizincicola is harvested in one time can be achieved in a one-time cultivation mode, meanwhile, high-quality cynodon dactylon turf cultivation is achieved in the soil remediation process, and the produced turf can be sold after remediation is completed; and economic benefits are created, and the soil remediation time and economic cost are greatly saved. Compared with an original cadmium-polluted soil remediation technical scheme, the method improves the remediation efficiency by 3-5 times, and reduces the remediation cost by 3 times or more.

The invention provides an insecticide for preventing and treating main pests attacking sedum plumbizincicola. The insecticide is characterized by comprising an emamectin benzoate thuringiensis insecticide and an emamectin benzoate hexaflumuron insecticide, wherein the dosage of the emamectin benzoate thuringiensis insecticide is 20-30 ml/mu, the dosage of the emamectin benzoate hexaflumuron insecticide is 10-20 ml/mu, and the emamectin benzoate thuringiensis insecticide and the emamectin benzoate hexaflumuron insecticide are mixed to prepare the mixed insecticide. Compared with a planting method in which mixed insecticide is not sprayed, the diluent of the mixed insecticide is used for spraying sedum plumbizincicola plants, so the insect attack phenomenon of sedum plumbizincicola can be effectively prevented and treated, the biomass of sedum plumbizincicola is remarkably improved, absorption and restoration of heavy metal pollutants are promoted, and restoration efficiency is effectively improved.

The invention discloses creation of heavy metal super-enriched genetically-engineered oryza sativa genetically modified with a Sedum plumbizincicola SpNramp5 gene. The creation comprises steps as follows: the SpNramp5 gene in Sedum plumbizincicola is cloned; based on a plant expression vector, a transformation vector containing a strong promoter and a screening gene is established, the SpNramp5 gene is inserted into the transformation vector with a homologous recombination method, and an overexpression binary vector is obtained; the overexpression binary vector is transformed into callus tissue of the oryza sativa by agrobacterium tumefaciens, genetically-modified seedlings are cultured, and genetically-modified positive plants are screened, wherein the transgenic receptor plants are the variety of oryza sativa Nipponbare. According to the culture method, the problems of limited endurance, slow growth, small biomass, special growing environment, heavy metal return and the like of a wild heavy metal enriched plant can be solved. Besides, the invention further provides an application of the heavy metal super-enriched genetically-modified plant obtained through the creation of the heavy metal super-enriched genetically-engineered oryza sativa genetically modified with the Sedum plumbizincicola SpNramp5 gene to restoration of heavy metal contaminated soil.

The invention discloses creation of heavy metal super-enriched genetically-engineered rape genetically modified with a Sedum plumbizincicola SpHMA3 gene. The creation comprises steps as follows: the SpHMA3 gene in Sedum plumbizincicola is cloned; based on a plant expression vector, a transformation vector containing a strong promoter and a screening gene is established, the SpHMA3 gene is insertedinto the transformation vector with a homologous recombination method, and an overexpression binary vector is obtained; the overexpression binary vector is transformed into a hypocotyl segment of therape by agrobacterium tumefaciens, genetically-modified seedlings are cultured, and genetically-modified positive plants are screened, wherein the transgenic receptor plants are cabbage type rape K407. According to the culture method, the problems of limited endurance, slow growth, small biomass, special growing environment, heavy metal return and the like of a wild heavy metal enriched plant canbe solved. Besides, the invention further provides an application of the heavy metal super-enriched genetically-modified plant obtained through the creation of the heavy metal super-enriched genetically-engineered rape genetically modified with the Sedum plumbizincicola SpHMA3 gene to restoration of heavy metal contaminated soil.