The invention discloses a paphiopedilum new variety 'yo-yo paphiopedilum' group rapid reproduction method, and relates to a biological engineering plant tissue culture reproduction method. The method includes the following steps of: 1, artificial cross pollination; 2, sterile sowing and protocorm formation stage; 3, proliferation and differentiation culture stage; 4, seedling strengthening and rooting stage; 5, seedling hardening and transplanting. The 'yo-yo paphiopedilum' combines the excellent traits of parents, has the advantages of being compact in plant type, unique and elegant in flower shape, attractive in flower color, easy to cultivate, up to 50 days in average flowering stage and the like, and avoids the disadvantages of being single in flower amount and too long in scape which is prone to be in lodging and broken. According to the method, the seed germination rate and the flowering rate are high, and the cultivation period is 3-4 years. The method is a high efficiency test tube reproduction method for obtaining a large number of 'yo-yo paphiopedilum' seedlings, and has the advantages of being high in germination rate, fast in seedling formation, good in seedling quality, strong in growth potential and the like, can obtain consistent test tube seedlings, and provides a large number of the seedlings in a short time so as to better protect wild resources.