Paphiopedilum new variety 'yo-yo paphiopedilum' group rapid reproduction method

Abstract

The invention discloses a paphiopedilum new variety 'yo-yo paphiopedilum' group rapid reproduction method, and relates to a biological engineering plant tissue culture reproduction method. The method includes the following steps of: 1, artificial cross pollination; 2, sterile sowing and protocorm formation stage; 3, proliferation and differentiation culture stage; 4, seedling strengthening and rooting stage; 5, seedling hardening and transplanting. The 'yo-yo paphiopedilum' combines the excellent traits of parents, has the advantages of being compact in plant type, unique and elegant in flower shape, attractive in flower color, easy to cultivate, up to 50 days in average flowering stage and the like, and avoids the disadvantages of being single in flower amount and too long in scape which is prone to be in lodging and broken. According to the method, the seed germination rate and the flowering rate are high, and the cultivation period is 3-4 years. The method is a high efficiency test tube reproduction method for obtaining a large number of 'yo-yo paphiopedilum' seedlings, and has the advantages of being high in germination rate, fast in seedling formation, good in seedling quality, strong in growth potential and the like, can obtain consistent test tube seedlings, and provides a large number of the seedlings in a short time so as to better protect wild resources.

Description

technical field [0001] The invention relates to a bioengineering plant tissue culture and propagation method, in particular to a tissue culture and rapid propagation method of a new Paphiopedilum species "Youyou Paphiopedilum". Background technique [0002] Orchidaceae is one of the largest families in nature and one of the largest and highly evolved groups of flowering plants. It is the third largest family in angiosperms after Compositae and Poaceae. There are about 700 genera and 20,000-30,000 species of orchids in the world, which are widely distributed in temperate, subtropical and tropical regions. More than 1,300 species of orchids have been discovered and named in more than 170 genera in my country, of which 2 / 3 are epiphytic orchids and 1 / 3 are terrestrial orchids. At present, the entire Orchidaceae has been included in Appendix I or II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Guizhou is a plateau province with widely...

AI Technical Summary

Problems solved by technology

[0008] In order to solve the technical problem in the prior art that "P. paphiopedilum" is difficult to reproduce in the natural state due to incomplete embryo development, the present invention provides a high-efficiency test tube propagation method with a low abortion rate and can greatly increase the reproduction coefficient , that is, the method of tissue culture and rapid propagation of a new species of Paphiopedilum "Youyou Paphiopedilum"

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