The invention discloses a solid culture medium for callus seedlings of stem tips of potatoes, which belongs to the technical field of agricultural biotechnology, comprising the following components: potassium nitrate, ammonium nitrate, monobasic potassium phosphate, magnesium sulphate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodiummolybdate dehydrate, copper sulfate, cobaltous chloride, ferric sulfate, ethylenediaminetetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extract, sucrose, 6-benzylaminopurine, zeatin, indole-3-acetic acid, 3-indolebutyric acid, gibberellin and polygel solidified medium. The culture medium is used for cultivating the stem tips of the potatoes, has good testing effect, basically has no callus tissues, and has high seedling rate. The invention greatly reduces the heteromorphosis of the regeneration plants of the stem tips, and ensures the integral primordial behaviors and the genetic background of the potato varieties. Compared with the primary method, because the culture method adopts the one-step seedling method, the method reduces the culture time and reduces the pollution probability.