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Solid culture medium for callus seedlings of stem tips of potatoes

A potato and culture medium technology, applied in the field of agricultural biology, can solve the problems of increasing the probability of pollution, plant variation, prolonging the cultivation time, etc., and achieve the effect of shortening the cultivation time and quickly growing seedlings

Inactive Publication Date: 2012-04-11
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two disadvantages in this process. One is that it is very easy to produce callus, and the plants regenerated through callus may mutate, and these mutations may change the excellent traits of the variety itself; Increased incubation time increases the chance of contamination

Method used

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  • Solid culture medium for callus seedlings of stem tips of potatoes
  • Solid culture medium for callus seedlings of stem tips of potatoes
  • Solid culture medium for callus seedlings of stem tips of potatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A kind of medium that is used for potato shoot tips without callus:

[0059] (1) Inorganic substances: potassium nitrate 1900mg / L, ammonium nitrate 1650mg / L, potassium dihydrogen phosphate 170mg / L, magnesium sulfate 370mg / L, calcium chloride 440mg / L, potassium iodide 0.83mg / L, boric acid 6.0mg / L , manganese sulfate 16.9mg / L, zinc sulfate 9.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, iron sulfate 27.8mg / L, ethylenediaminetetraacetic acid Two sodium 37.3mg / L;

[0060] (2) Organic matter: inositol 100mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, thiamine hydrochloride 0.1mg / L, glycine 2mg / L;

[0061] (3) Plant hormones: 6-benzylaminoadenine 0.2mg / L, indole butyric acid 0.05mg / L, gibberellin 0.1mg / L;

[0062] (4) Sucrose 30g / L;

[0063] (5) Biliconate 4g / L.

[0064] Preparation method: Accurately weigh each component according to the recipe, add deionized water to fully dissolve, constant volume, adjust pH to 5.8-6.0,...

Embodiment 2

[0067] A kind of medium that is used for potato shoot tips without callus:

[0068] (1) inorganic matter: with embodiment 1;

[0069] (2) organic matter: with embodiment 1;

[0070](3) Plant hormones: 6-benzylaminoadenine 0.1mg / L, indolebutyric acid 0.2mg / L, gibberellin 0.2mg / L;

[0071] (4) Sucrose 30g / L;

[0072] (5) Biliconate 4g / L.

[0073] Configuration method: same as embodiment 1.

Embodiment 3

[0075] A kind of medium that is used for potato shoot tips without callus:

[0076] (1) inorganic matter: with embodiment 1;

[0077] (2) organic matter: with embodiment 1;

[0078] (3) Phytohormone: Zeatin 0.5mg / L, Gibberellin 1.0mg / L;

[0079] (4) Sucrose 30g / L;

[0080] (5) Biliconate 4g / L.

[0081] Configuration method: same as embodiment 1.

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Abstract

The invention discloses a solid culture medium for callus seedlings of stem tips of potatoes, which belongs to the technical field of agricultural biotechnology, comprising the following components: potassium nitrate, ammonium nitrate, monobasic potassium phosphate, magnesium sulphate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodiummolybdate dehydrate, copper sulfate, cobaltous chloride, ferric sulfate, ethylenediaminetetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extract, sucrose, 6-benzylaminopurine, zeatin, indole-3-acetic acid, 3-indolebutyric acid, gibberellin and polygel solidified medium. The culture medium is used for cultivating the stem tips of the potatoes, has good testing effect, basically has no callus tissues, and has high seedling rate. The invention greatly reduces the heteromorphosis of the regeneration plants of the stem tips, and ensures the integral primordial behaviors and the genetic background of the potato varieties. Compared with the primary method, because the culture method adopts the one-step seedling method, the method reduces the culture time and reduces the pollution probability.

Description

technical field [0001] The invention relates to a solid culture medium for forming potato seedlings without callus, and belongs to the field of agricultural biotechnology. Background technique [0002] Potato (Solanum tuberosum L.) originated in the Andes of South America and was introduced to China in the 1670s. It has been cultivated in China for more than 400 years. It is the fourth largest crop in the world and the fifth largest crop in China, with extensive planting area in China and the world. The starch content of potato is as high as 12%-20%, the protein content is 1.5%-2%, and the Vc content is 10-20mg / 100g fresh weight. An important raw material that is missing. [0003] Potato plants are susceptible to various viruses during production. After the potato is infected with the virus, the growth vigor and yield of the plant decrease generation by generation, and in severe cases there is almost no commercial output. Therefore, after a few generations of potatoes, t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘芳杨元军王培伦马伟清董道峰陈广侠马蕾
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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