Solid culture medium for callus seedlings of stem tips of potatoes
A potato and culture medium technology, applied in the field of agricultural biology, can solve the problems of increasing the probability of pollution, plant variation, prolonging the cultivation time, etc., and achieve the effect of shortening the cultivation time and quickly growing seedlings
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[0057] Example 1
[0058] A medium for potato shoots to grow without callus:
[0059] (1) Inorganic substances: potassium nitrate 1900mg / L, ammonium nitrate 1650mg / L, potassium dihydrogen phosphate 170mg / L, magnesium sulfate 370mg / L, calcium chloride 440mg / L, potassium iodide 0.83mg / L, boric acid 6.0mg / L , Manganese sulfate 16.9mg / L, Zinc sulfate 9.6mg / L, Sodium molybdate 0.25mg / L, Copper sulfate 0.025mg / L, Cobalt chloride 0.025mg / L, Ferric sulfate 27.8mg / L, Ethylenediaminetetraacetic acid Two sodium 37.3mg / L;
[0060] (2) Organic matter: inositol 100mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, thiamine hydrochloride 0.1mg / L, glycine 2mg / L;
[0061] (3) Plant hormones: 6-benzylamino adenine 0.2mg / L, indolebutyric acid 0.05mg / L, gibberellin 0.1mg / L;
[0062] (4) Sucrose 30g / L;
[0063] (5) Baili Ning 4g / L.
[0064] Preparation method: Accurately weigh each component according to the formula, add deionized water to fully dissolve it, make the volume constant, adjust the pH to 5...
Example Embodiment
[0066] Example 2
[0067] A medium for potato shoots to grow without callus:
[0068] (1) Inorganic: the same as in Example 1;
[0069] (2) Organic matter: the same as in Example 1;
[0070] (3) Plant hormones: 6-benzylamino adenine 0.1 mg / L, indole butyric acid 0.2 mg / L, gibberellin 0.2 mg / L;
[0071] (4) Sucrose 30g / L;
[0072] (5) Baili Ning 4g / L.
[0073] Configuration method: same as Example 1.
Example Embodiment
[0074] Example 3
[0075] A medium for potato shoots to grow without callus:
[0076] (1) Inorganic: the same as in Example 1;
[0077] (2) Organic matter: the same as in Example 1;
[0078] (3) Plant hormones: Zeatin 0.5mg / L, Gibberellin 1.0mg / L;
[0079] (4) Sucrose 30g / L;
[0080] (5) Baili Ning 4g / L.
[0081] Configuration method: same as Example 1.
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