Method for crossbreeding and aseptic sowing of renanthera coccinea

A technology of aseptic sowing and hybrid breeding, applied in botany equipment and methods, soilless cultivation, plant genetic improvement, etc., can solve the problems of pollen preservation failure, mildew, and no problem in flowering, and achieve high germination rate and seedling The effect of good seedling quality and strong operability

Inactive Publication Date: 2011-08-10
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the flame orchid has a high hybrid affinity, it has the problem of not meeting the flowering period when it is hybridized with other orchids. At the same time, some distant hybrids cannot be carried out due to the not meeting the flowering period. In addition, it has been reported that low temperature preservation of pollen of other plants often occurs Pollen preservation failure caused by mildew

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Hybrid Breeding and Seedling Propagation of Doritis pulcherrima and Renanthera imschootiana

[0025] (1) Artificial pollination: Yunnan Flame Orchid blooms in early March, collect pollen when its single flower petals just unfold, use a sterilized toothpick to gently pick off the medicinal cap of the flower at 8-9 in the morning, and then use the toothpick to remove the pollen. The pollen block sticks to the tip of the toothpick and quickly put the pollen block into a clean and sterile 1ml EP tube (centrifuge tube), put the pollen block of 1 flower in each tube, put the pollen block in the EP tube and use a sterile The glass bottles were packed together and sealed well, and finally the glass bottles filled with pollen were placed at 4°C and protected from light. After the flame orchid pollen was preserved for 3 months, when the female parent orchid orchid bloomed in early June of that year, the pollen of the flame orchid preserved at low temperature was thawed and rehumi...

Embodiment 2

[0031] Cross Breeding and Seedling Propagation of Vanda coerulescens and Renanthera imschootiana

[0032] (1) Artificial pollination: Yunnan Flame Orchid blooms in early March. Collect pollen when the petals of its single flower have just unfolded. The toothpick sticks the pollen block to the tip of the toothpick and quickly puts the pollen block into a clean and sterile 1ml EP tube, puts the pollen block of one flower in each tube, puts the pollen block in the EP tube and concentrates it in a sterile glass bottle Pack it up and seal it well, and finally place the glass bottle filled with pollen at 4°C and keep it away from light. After the flame orchid pollen has been stored for 6 months, when the female parent floret Vanda orchid blooms in early September of that year, the low-temperature preserved flame orchid pollen will be thawed and rehumidified to restore and improve the vitality of the pollen. First thaw the flame orchid pollen stored at low temperature at room temper...

Embodiment 3

[0038] Hybrid Breeding and Seedling Propagation of Cymbidium ensifolium and Yunnan Flame Orchid (Renanthera imschootiana)

[0039] (1) Artificial pollination: Yunnan Flame Orchid blooms in early March. Collect pollen when the petals of its single flower have just unfolded. The toothpick sticks the pollen block to the tip of the toothpick and quickly puts the pollen block into a clean and sterile 1ml EP tube, puts the pollen block of one flower in each tube, puts the pollen block in the EP tube and concentrates it in a sterile glass bottle Pack it up and seal it well, and finally place the glass bottle filled with pollen at 4°C and keep it away from light. After the flame orchid pollen has been preserved for 9 months, when the female parent Caixin Jianlan blooms in early December of that year, the low-temperature preserved flame orchid pollen will be thawed and rehumidified to restore and improve the vitality of the pollen. Thaw the flame orchid pollen stored at low temperatur...

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PUM

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Abstract

The invention discloses a method for crossbreeding and aseptic sowing of renanthera coccinea, comprising the following steps of: carrying out hybridization by selecting orchid with obvious characters and high visual value as a hybrid female parent and renanthera coccinea as a male parent; carrying out aseptic sowing on a seed germination medium on the fruit obtained by successful pollination when being basically ripened but not splitted; after germinating, transferring the seeds to a healthy seedling medium; and growing the formed seedling in a test tube out of a bottle after being hardened under the illumination of natural light for 10 to 20 days, wherein the transplanting survival rate of seedling formation can reach 90% or more. The method has the characteristic of high hybrid success rate, and is particularly useful during taking orchid with obvious characters and high visual value as a hybrid male parent which is different from a renanthera coccinea parent in flowering phase. The unique medium and cultivation method are employed by the invention so that the hybrid seeds of renanthera coccinea have high germination, fast seedling formation and good seedling quality. The method has the advantages of strong operation, effective breeding, valuable application and the like.

Description

Technical field: [0001] The invention relates to a plant hybrid breeding method, in particular to a flame orchid hybrid breeding and aseptic sowing method. Background technique: [0002] There are about 21 species of Reanathera plants in the world, which are epiphytic or semi-epithytic plants. They are distributed from Southeast Asia to the Himalayas, including South China. There are 3 species native to my country, of which Ren. Coccinea is produced in In Guangdong and Hainan, the plants grow tall and vigorous. Yunnan Flame Orchid (Ren.imschootiana) and Chinese Flame Orchid (Ren. sinica) are produced in Yunnan. Flame orchid grows vigorously, has bright colors, numerous flowers, and looks like a flame from a distance. It has high horticultural application value and breeding value. It is a very popular high-end flower in the international flower market and has a large number of lovers in the world. And because its growth environment is tropical and subtropical, it is suitable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01G31/02A01G1/00
CPCY02P60/21
Inventor 吴坤林曾宋君陈之林段俊张建霞唐源江
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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