Method for increasing seedling survival rate of intergroup distant hybrid seeds of paeonia

A technology of distant hybridization and Paeonia genus, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve the problem of low seedling emergence rate, obvious difference in the effect of in vitro embryo culture of different genotypes, and unidentified seeds that are difficult to germinate and other issues to achieve the effect of improving the seedling rate

Active Publication Date: 2019-04-05
BEIJING FORESTRY UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] Table 2 shows the research on seedling cultivation of isolated peony embryos. The research objects are relatively limited, mainly purple spot peony or 'Fengdan'. The seedling cycle and seedling rate are different under different schemes, and the shortest seedling cycle is 40 Today, the seedling rate can reach as high as 93.75%, but there are few data on the survival rate of transplanting. Without the data on the survival rate of transplanting, it is difficult to prove the feasibility of the scheme. At present, the most efficient and fast solution of this technology is the purple spotted peony: improved MS (double calcium, double macroelements)+0.6g / L AC+0.5mg / LGA, 'Fengdan': Improved MS (double calcium, double macroelements)+0.6g / L AC+1.0mg / L GA, available at Within 40 days, the seedling rate can reach 93.75% and 63.88%; and the transplanting survival rate can reach up to 100% and 66.34% (Xu Li, 2017)
From the difference between the purple spot peony and 'Fengdan', it can be seen that the effect of in vitro embryo culture of different genotypes is significantly different. This program has reference value for the embryo culture of other genotypes, but it needs to be adjusted and improved according to the specific situation. See a successful case of applying this technology in the field of distant cross breeding
[0005] To sum up, there are the following deficiencies in the breeding of traditional seedling raising methods: 1. Low emergence rate: the traditional seedling raising method does not distinguish between seeds with complete seed coats and cracks when sowing, and does not identify seeds that are difficult to germinate; it is greatly affected by natural conditions, such as Rainfall, temperature, light, soil conditions, etc.
2. Long breeding cycle: Under the traditional seedling raising method, it takes about 3 months for seeds to germinate and take root, and the upper part of the next spring will germinate true leaves. The process of hybrid identification in the later stage is slow and the breeding cycle is long
3. The in vitro embryo culture system is still to be perfected, and there is no precedent for applying it to the distant hybrid breeding of Paeoniae genus

Method used

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  • Method for increasing seedling survival rate of intergroup distant hybrid seeds of paeonia
  • Method for increasing seedling survival rate of intergroup distant hybrid seeds of paeonia
  • Method for increasing seedling survival rate of intergroup distant hybrid seeds of paeonia

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Obtaining and screening of hybrid seed of embodiment 1

[0053] 1. Experimental materials

[0054] It was carried out in 2016 and 2017 at the Peony and Paeoniae Research Base of Beijing Forestry University, Jiufeng National Forest Park, Haidian District, Beijing. The female parent used in the hybridization is P.Lactiflora'Martha W.', the male parent is P.×lemoineii'Golden Era', and the high-generation hybrid P.×lemoineii(07-LB-1) between the Mudan subgroups (Cao Xijun, 2007) and the distant hybrid P.×lemoineii 'Black Pirate' bred in the United States.

[0055] 2. Hybrid pollination

[0056] Conventional methods for cross pollination. The pollen of the male parent was collected in late April. When the flower buds of the male parent are in the dew stage, the anthers are collected and dried in the shade indoors to loose powder. The pollen was collected in a clean centrifuge tube, and the variety name was marked and stored in a -20°C refrigerator. Remove the side buds...

Embodiment 2

[0060] The screening of embodiment 2 isolated embryo culture conditions

[0061] 1. Experimental materials

[0062] Naturally hybridized purpura seeds.

[0063] 2. Experimental method

[0064] Wash the purpura seeds, rinse them with running water for 15 minutes, rinse them with detergent for 15 minutes, rinse them with tap water for 15 minutes, and place them on an ultra-clean workbench. Disinfect by immersing in 70% alcohol for 1 min, then sterilize by shaking twice with 5% sodium hypochlorite solution, and then rinse with sterile water 5 times. Use tweezers to take out the seed embryo and inoculate it in the embryo seedling medium, culture temperature 25±4℃, photoperiod 14h·d -1 , light intensity 32.4umol m -2 ·s -1 .

[0065] ①Effects of different hormones on embryo growth

[0066] Different hormone combinations were used to study their effects on the growth of embryos, respectively CK (without hormone), 0.25mg / L IAA, 0.25mg / L IAA+0.5mg / L GA 3 , 0.5mg / L GA 3 , 0.25...

Embodiment 3

[0075] The detached embryo of embodiment 3 cracking hybrid seed is cultivated into seedling

[0076] 1. Experimental materials

[0077] Dehiscented seeds of distant hybrids between groups of Paeoniae.

[0078] 2. Experimental method

[0079] ①Remove the seed coat and wash the seeds with cracked seed coat. When removing the seed coat, you can keep the hilum point according to the specific situation. The rest of the disinfection steps are the same as above. Roll on a clean filter paper, absorb moisture by the filter paper and make it dry, then place it on another clean filter paper for operation, find the best anatomical position according to the shape of the seed and the position of the umbilical point, and take out the seed embryo.

[0080] ②Use tweezers to take out the plump seed embryos of the seed coat cracking harvested in the distant hybridization between groups and inoculate them in the best-treated seedling growth medium obtained in Example 2, and count the embryo ger...

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Abstract

The invention provides a method for increasing the seedling survival rate of intergroup distant hybrid seeds of paeonia. The method comprises the steps that intergroup hybrid seeds with very low seedling survival rate are screened through the character of seed coat cracking, and rapidly cultured into seedlings by adopting an in-vitro embryo culture technology, thereby increasing the seedling survival rate of the intergroup hybrid seeds and shortening the breeding cycle. Research finds that the combination of IAA, GA3 and activated carbon has a promoting function on in-vitro seedling survival of seed embryos, and a best in-vitro embryo seedling survival culture medium is obtained. Seed embryo root systems can rapidly elongate under the culture medium, true leaves germinate, so that the seedembryos can rapidly grow into seedlings, the average length of the true leaves of the embryo seedlings reaches 2.49 cm, and the average length of the roots reaches 4.74 cm. In a traditional seedlingraising mode, seeds of seed coat cracking with the very low seedling survival rate can rapidly grow into seedlings within 40 days, blades of the seedlings after transplanting survival are large, and the growth vigor is strong, thereby increasing the whole seedling survival rate of the distant hybrid seeds, and accelerating the breeding process of new varieties.

Description

technical field [0001] The invention relates to the field of tissue culture technology and breeding, in particular to a method for improving the seedling rate of distant hybrid seeds between groups of Paeoniae genus. Background technique [0002] Peony (Paeonia sect. Moutan) is a traditional famous flower in my country. It has large and colorful flowers and has the reputation of "national beauty and heavenly fragrance". ), peony group (Sect.Paeonia) and North American peony group (Sect.Onaepia) (Hong Deyuan, 1999). The peony group is further divided into two subgroups, subsect.Vaginatae and subsect.Delavayanae (subsect.Delavayanae) (Wang Lianying et al., 1997; Li Jiajue, 1999). Intergroup hybridization refers to the distant hybridization between the peony group and the peony group. Compared with the close hybridization within the subgroup or the variety group, the intergroup hybridization is more difficult. The offspring obtained from this type of hybrid combination are int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/15A01G24/28
CPCA01G31/00A01H4/001A01H4/008A01G24/15A01G24/28
Inventor 钟原徐莉成仿云杜明杰
Owner BEIJING FORESTRY UNIVERSITY
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