Louis iris tissue culture fast seedling establishment and ecological application method

Abstract

The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.

Description

technical field [0001] The invention relates to a method for rapid propagation and ecological application of aquatic plants, in particular to a method for rapid seedling growth and ecological application of iris louis. Background technique [0002] Under natural conditions, Iris louis is mainly propagated by ramets or seeds. Although ramet propagation is simple and easy, a single plant can only be divided once a year, and the reproductive rate is generally only 10 times, which is relatively low and is not conducive to mass reproduction. Applied to a long ecological cycle. [0003] Although most iris varieties can achieve the purpose of rapid propagation through tissue culture, the conventional tissue culture has a relatively long period from test tube seedlings to ecological application, and the survival rate cannot be guaranteed. Contents of the invention [0004] The technical problem to be solved by the present invention is to overcome the above-mentioned defects of th...

AI Technical Summary

Problems solved by technology

[0002] Under natural conditions, Iris louis is mainly propagated by ramets or seeds. Although ramet propagation is simple and easy, a single plant can only be rametted once a year, and the reproductive rate is generally only 10 times, which is relatively low and is not conducive to mass reproduction. Applied to a long ecological cycle

[0003] Although most iris varieties can achieve the purpose of rapid reproduction through tissue culture, the conventional tissue culture has a long cycle from test tube seedlings to ecological application, and the survival rate cannot be guaranteed