Method for breeding and cultivating seedlings of cypripedium corrugatum with red glows

A technology of Hongxia and seedlings, which is applied in the field of ornamental plant reproduction and cultivation, can solve the problems of difficult seed germination, difficult seed germination of Paphiopedilum japonica, etc., and achieves the effects of high seed germination rate, strong resistance and fast seedling formation.

Inactive Publication Date: 2018-11-09
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, it is difficult to germinate seeds of Paphiopedilum under natural conditions, and the basic medium of Huabao No. 1, 1 / 2MS, and MS commonly used in aseptic sowing technology also has difficulties in seed germination.

Method used

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  • Method for breeding and cultivating seedlings of cypripedium corrugatum with red glows
  • Method for breeding and cultivating seedlings of cypripedium corrugatum with red glows
  • Method for breeding and cultivating seedlings of cypripedium corrugatum with red glows

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Embodiment 1

[0042] A method for seedling propagation and cultivation of Paphiopediphyllum erythrophyllum, comprising the following aspects:

[0043] (1) Artificial pollination: with P. chinensis (flowering plants such as figure 1 Shown) is the female parent, and Paphiopedilum of the same color is the male parent (flowering plants such as figure 2 As shown), the pollen of the male parent was collected with a sterilized toothpick and pollinated on the stigma of the female parent. Specifically, in the middle and late April, the female parent of P. Remove the lip petals of the female parent and the male parent, and infuse the pollen of the same color Papudlum chinensis on the stigma of the Paphiopedilum pallidum. 120 days after pollination, the basically mature and uncracked capsules were used as explants, such as image 3 shown.

[0044] (2) Preservation of hybrid capsules: the collected capsules were packed in nylon mesh bags and stored in a refrigerator at 4°C for 5 days.

[0045] (3)...

Embodiment 2

[0055] A method for seedling propagation and cultivation of Paphiopediphyllum erythrophyllum, comprising the following aspects:

[0056] (1) Artificial pollination: take Paphiopedilum pachyphyllum as the female parent and the same color Paphiopedilum as the male parent, collect pollen from the male parent with a sterilized toothpick and confer it on the stigma of the female parent. Capsules serve as explants.

[0057] (2) Preservation of hybrid capsules: the collected capsules were packed in nylon mesh bags and stored in a refrigerator at 4°C for 3 days.

[0058] (3) Disinfection of hybrid capsules: dip the treated capsules in step (2) on an ultra-clean workbench with cotton balls dipped in 70% alcohol to wipe the surface of the capsules to dry, then disinfect with 0.2% mercuric chloride solution for 18 minutes, and then sterilize Rinse with water 5 times.

[0059] (4) Aseptic seeding and protocorm induction: cut the sterilized capsules with a blade, inoculate the brown powd...

Embodiment 3

[0072] A method for seedling propagation and cultivation of Paphiopediphyllum erythrophyllum, comprising the following aspects:

[0073] (1) Artificial pollination: take Paphiopedilum pachyphyllum as the female parent and the same color Paphiopedilum as the male parent, collect pollen from the male parent with a sterilized toothpick and confer on the stigma of the female parent. Capsules serve as explants.

[0074] (2) Preservation of hybrid capsules: the collected capsules were packed in nylon mesh bags and stored in a refrigerator at 4°C for 4 days.

[0075] (3) Disinfection of hybrid capsules: dip the treated capsules in step (2) on an ultra-clean workbench with cotton balls dipped in 70% alcohol to wipe the surface of the capsules and dry them, then disinfect them with 0.1% mercuric solution for 22 minutes, and sterilize Rinse with water 5 times.

[0076] (4) Aseptic seeding and protocorm induction: cut the sterilized capsules with a blade, inoculate the brown powder see...

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Abstract

The invention discloses a method for breeding and cultivating seedlings of cypripedium corrugatum with red glows. The method includes the steps of artificial pollinating, collection and storage of hybridized capsules, sterilization of the hybridized capsules, sterile sowing and protocorm inducing, strong seedling culture and rooting culture, acclimatization and transplant, transplant for greenhouse cultivation, and disease and pest control. In artificial pollinating, paphiopedilum hirsutissimum is taken as a female parent, and paphiopedilum concolor is taken as a male parent. A culture mediumfor seed germination is prepared from 1/5-1/6 of MS, 1.0-2.0mg/L of 6-benayl aminopurine, 0.05-0.50mg/L of naphthylacetic acid, 10.0-15.0% of coconut juice, 10.0-15.0% of banana juice, 1.0-1.5g/L of activated carbon, 20-30g/L of cane sugar and 9.5-10.0g/L of carrageenan powder. The method has the advantages that seed germination rate and seedling formation speed are high, the seedlings grow vigorously, and the problem that hybridizing seeds with the paphiopedilum hirsutissimum as the female parent and the paphiopedilum concolor as the male parent to germinate is hard in a natural state is solved.

Description

technical field [0001] The invention relates to the cultivation of Paphiopedilum, in particular to a seedling propagation and cultivation method of Paphiopedilum, and belongs to the technical field of propagation and cultivation of ornamental plants. Background technique [0002] Paphiopedilum, also known as slipper orchid in horticulture, is a general term for plants of the genus Paphiopedilum in the family Orchidaceae. Its long-lasting flowering period, gorgeous flower color and peculiar flower shape have high ornamental and economic value. Paphiopedilum is mainly distributed in tropical Asia to some islands in the Pacific Ocean. It is a terrestrial orchid, and a few are epiphytic orchids; China is one of the countries with the most abundant plants of the genus Paphiopedilum in the world (Liu Zhongjian et al., 2009). Due to the over-exploitation of wild Paphiopedilum resources, Paphiopedilum has become one of the most endangered plant species in the world, and all wild Pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 李冬梅朱根发赵超艺刘小飞
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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