35 results about "Endangered plant species" patented technology

The invention relates to a method of artificial fast breeding for spun gold suspended gourd, with the detail steps in below: 1) pretreating the explant, 2) sterilizing the explant, 3) inoculating, 4) inducing culturing, 5) differentiation culture for callus, 6) enrichment culture, 7) strengthening sprout culture, 8) root-growing culture, 9) sprouting training and replanting, 10) sprout bed management, 11) bedding in earth the culture sprout, 12) shearing the cuttage ear, 13) treating the cuttage ear with medical agent, 14) cuttage, 15) cuttage sprout bed management. The benefit of this invention is that:: by making use of the advantage of plant tissue culture technique to breed large quantity of spun gold suspended gourd tissue culture sprout in tube with a high speed, the monthly breeding rate is 3-5 times, the yearly tissue culture sprout producing ability is more than ten thousand per square meter, and it solves the problem of spun gold suspended gourd resource shortage. The provided artificial fast breeding method uses less explant material, which can effectively save rare endangered plant species, and the mass production can be carried out which can provide seed foundation for the further development of spun gold suspended gourd product.

The invention discloses a seedling growing method for ammodendron argenteum. The method comprises the following steps that of 1 seedling growing land selection and treatment, 2 seed selection and treatment, 3 sowing treatment, 4 seedling stage management and 5 seedling outplanting. The method has the advantages that the seedling growing method for the rare or endangered species, namely, ammodendron argenteum is provided, the provided seedling growing mode is high in operability, seedlings emerge orderly, the survival rate is high in the process of transplanting and foresting, and the seedlings are good in growth vigor, normal in color and free of mechanical damage and plant diseases and insect pests.

The excised white laurel propagating process consists of the following steps: obtaining aseptic explant, inducing bud, proliferating bud, inducing and culturing root, and transplanting test seedling. The present invention is excised tissue culture and fast propagation technology for providing excellent tissue cultured seedlings of white laurel as one kind of endangered plant species.

The invention belongs to the technical field of biology, and particularly relates to a rare and endangered plant Rhododendron henanense seed seedling artificial breeding method. The rare and endangered plant Rhododendron henanense seed seedling artificial breeding method comprises the following steps that mature fruits of Rhododendron henanense are collected, and capsules are dissected to obtain mature seeds for later use; a culture dish double-layer filter paper method is adopted, and first-time seedling hardening is carried out after most of the seeds germinate; when the seeds are cultured, the germination temperature is set to be 15-30 DEG C, illumination is set to be 14 h/d, and the illumination intensity is set to be 10000-20000 lx; waiting for about 3 days to see partial germination of the seeds; first-time seedling hardening is carried out on the sixth day to the ninth day after seed germination, and seedlings with two cotyledons grow in 3-4 days; and culturing is continuously carried out. By utilizing the artificial breeding method, the germination rate of the Rhododendron henanense seeds is high, the highest germination rate can exceed 94%, the survival rate of seedlings is high, the bred seedlings grow well, the rare and endangered Rhododendron henanense can be protected, the Rhododendron henanense can be developed and utilized as an ornamental flower variety, and a technical foundation is laid for effectively rescuing the endangered species.

The invention provides a germplasm storage method for East Asian phyllitis japonica, which comprises the following steps that: prothallus is inoculated in a 1/4-MS (full) + sucrose 25-30 g/L medium, and the medium is placed in an illumination incubator, wherein the illumination intensity is 200-300 lx, the illumination is 5-7 h/d, and the temperature is 14-16 DEG C; 12 months later, almost no growth and proliferation occurs, and the state of the prothallus is similar as that in initial culture, and after the prothallus is transferred into successive culture conditions, the prothallus can proliferate and grow normally; and the prothallus is subjected to five-year storage treatment. The invention also provides an ex-situ conservation method for East Asian phyllitis japonica, which is implemented through a way that the stored prothallus is subjected to sub-cultured reproduction every year, and the formation and ex-situ transplanting of sporophore plants are induced by taking turfs as a matrix outside a test tube; three sits with basically similar as the original growing environment of phyllitis japonica are selected as ex-situ transplanting test areas in Changbai Mountains; 100 plants are planted at each site; and after growing 4 years, the growth vigour of the prothallus is good, thereby truly realizing the protection on endangered plants.

The invention discloses a method for increasing the germination rate of seeds of threatened plant gymnadenia cono-psea. The method includes the steps of firstly, conducting pretreatment; secondly, conducting cultivating through a germination culture medium, wherein after pretreatment, sterilization treatment is conducted, capsules are cut longitudinally through a sterile surgical knife blade, seeds are picked out and dispersed in the germination culture medium, and inoculated seeds are cultured for 5 days under the dark condition at the temperature of 18 DEG C, continue to be cultured for 1 day under the dark condition at the temperature of 25 DEG C and then continue to be cultured under the dispersed light conditions of the luminance cycle of 12 h.d<-1> and the luminance intensity of 3.2 micromole.m<-2>.s<-1>. The germination culture medium is prepared from 11.8 g/L of cane sugar, 3.9 g/L of agar, 0.5 g/L of compound traditional Chinese medicine extracting solution, 8 g/L of coconut juice and 0.1 mg/L of 6-BA with MS as the basic culture medium and has the pH value of 6.5.

The invention provides a davidia involucrata ex-situ conservation technology. The davidia involucrata ex-situ conservation technology comprises the following steps that investigating is carried out before davidia involucrata ex-situ conservation, seeds are introduced step by step, transplanting is carried out step by step, a plurality of vegetation and associated tree species of an original growing land in an area are planted after davidia involucrata ex-situ conservation, and an environment with similar habitat of plants is created. The problem that the survival rate of wild davidia involucrata in a high-altitude area is low after the wild davidia involucrata migrates to a low-altitude area is solved, a new high number and quality of ex-situ conservation of big davidia involucrata trees is created, the conservation level of rare and endangered plants is remarkably improved, and great reference significance for ex-situ conservation work of rare and endangered plants with gradually narrowed distribution range of ecological environments of other areas suffering from serious damage is achieved.

The invention relates to a plant tissue and cell culturing method, in particular to a method for producing calluses and high-quality suspension cell granules in a large scale by taking stems of a specific rare or endangered plant myricaria laxiflora in the Three Gorge basin as explants, belongs to the field of plant tissue or cell culture, and discloses a callus and suspension cell granule culturing method. The method comprises the following steps: 1, obtaining aseptic myricaria laxiflora plant body organs, and performing comparison to obtain parts with the most prominent overall performance in roots, stems and leaves of the system; 2, inoculating the aseptic stems into a callus inducing medium to induce and subculture calluses to obtain the calluses using myricaria laxiflora tissues as raw materials; 3, inoculating the calluses to a corresponding liquid medium, and performing shaking culturing to obtain stable-character suspension cell granules originated from the myricaria laxiflora plant body organs. The calluses are stable in character, the obtained suspension cell granules are high in growth speed and good in dispersity, cell clusters are smaller, and a solution is clear and transparent.

The invention relates to a cultivation method of endangered plant Tibetan paeonia ludlowii, in particular to a cultivation method of paeonia ludlowii, and aims to solve the problems of long germination time, low germination rate and low cultivation survival rate of paeonia ludlowii seeds. The cultivation method comprises the steps that 1, a plant hormone GA3 solution with the concentration of 100mg/L is prepared, paeonia ludlowii seeds are soaked with the GA3 solution, then the seeds treated with the plant hormone are sown into a ridged field, and the sowing depth is controlled; 2, enough base fertilizer is applied to the transplanting land, 5000-6000 Kg of animal manure is applied per mu, and seedlings are planted; and 3, soil loosening, weeding and topdressing are conducted in the fieldmanagement process. According to the method, the paeonia ludlowii seeds are treated through exogenous hormones, that is, the germination rate of the paeonia ludlowii seeds soaked with 100 mg/L of GA3for 48 h is 79-90%, the seedling rate can reach 75%-85%, and the survival rate of seedlings can reach 70%-80%.

The invention provides a method for promoting germination of seeds of endangered plant phoebe hainanensis, which comprises the working procedures of seed collection, seed selection and treatment, sand bed preparation, sowing, nutrient solution preparation and spraying and management after sowing, after phoebe hainanensis fruits are harvested, the seeds are taken out when peels crack, after the seeds are subjected to pest killing and water washing, the seeds with the weight of each seed being greater than or equal to 5.0 g are screened out, and the seeds with the weight being greater than or equal to 5.0 g are obtained. The method comprises the following steps of: soaking the seeds in a water bath at 35 DEG C for 6 hours, sowing the seeds in a prepared culture container, putting the culture container into a culture box after sowing, and spraying a nutrient solution to the culture container once at 8: 00-9: 00 every day. Aiming at the physiological characteristics of the phoebe hainanensis seeds, the morphological after-ripening process is completed under the condition of water bath at 35 DEG C for 6 hours, the spraying treatment of the nutrient solution is combined, the seed germination time is remarkably shortened, the germination rate is remarkably increased, the method has the characteristics of simplicity and convenience in operation and rapid and tidy germination, and the requirements of large-scale cultivation and standardized production of phoebe hainanensis are met.

The invention discloses an artificial multiplication method for carpinus tientaiensis, pertaining to the technical field of artificial multiplication for plants. The artificial multiplication method comprises following steps: (1) picking seeds; (2) storing seeds; (3) breaking dormancy of seeds; (4) selecting soil and culture mediums to grow seedlings; (5) transplanting of nursery stocks. The artificial multiplication method for carpinus tientaiensis has following beneficial effects: by utilization of the method, seeds of carpinus tientaiensis are subjected to mass propagation such that the dormancy characteristic for seeds of carpinus tientaiensis after ripening of embryo is discarded; breaking dormancy of seeds can be rapidly achieved for seeding growth so that germination percentage of seeds is increased by more than 50% and seedling survival percent can reach at more than 80%; the artificial multiplication method is in favor of increase in population of carpinus tientaiensis and has great scientific significance and practical significance in preventing carpinus tientaiensis as endangered plants against extinction.

The invention discloses a non-symbiotic germination method for seeds of rare and endangered plant calanthe davidiana. The method comprises the following steps: disinfection of explants: selecting mature and non-cracked capsules for disinfection; seed germination culture: taking out the seeds, uniformly sowing the seeds on a culture medium, and performing sterile germination under an illumination condition; inducing lateral bud differentiation culture: transferring the small pseudobulb without the leaves into a differential culture medium, and culturing under an illumination condition; rooting and seedling strengthening culture: when the differentiated seedlings grow 1-2 leaves and the length of the leaves is 2-3cm, transferring the seedlings into a rooting and seedling strengthening culture medium for culture; seedling hardening and transplanting: transplanting the tissue culture seedlings into a greenhouse for seedling hardening, taking out, cleaning, disinfecting and soaking; and transplanting the seedlings into a seedling-raising hole tray filled with a substrate. Mature and non-cracked capsules are selected as explants, disinfection, seed germination, lateral bud differentiation, rooting and seedling strengthening, seedling hardening and transplanting are sequentially carried out, culture parameters of all stages are optimized, and a large-scale propagation means and a scientific and effective protection method are established.