36 results about "Endangered plant species" patented technology

The invention discloses a method for promoting germination and repeated propagation of garcinia paucinervis seeds. The method can greatly shorten seedling period, increase the seedling yield of seeds and reduce seedling costs by comprehensively controlling various germination factor conditions of the garcinia paucinervis seeds, breaking seed dormancy, increasing a germination speed, uniformity and a germination rate of the seed, greatly promoting germination of the seeds, and also raising seedlings in multiple batches based by using a characteristic that the seeds can germinate repeatedly. The garcinia paucinervis seeds, having an original germination rate of 85.0% in 350 days, have an initial germination rate of up to 95.0% in 30 days after being processed by the method; the recycled seeds have the germination rate of up to 83.3% or more through germination acceleration for 60 days, and are fast and uniform in germination. The method lays a good foundation for efficient propagation of garcinia paucinervis seedlings, and has a very positive impact on regeneration and sustainable utilization of rare and endangered plant resources.

The invention discloses a method for tissue culture propagation and in vitro preservation of an extremely endangered plant Rhododendron guizhouensis. The method is suitable for tissue culture rapid propagation and in vitro preservation of Rhododendron guizhouensis in the family Rhododendronaceae, and belongs to the field of plant biotechnology. The present invention uses freshly drawn tender stems after flowering as explants, and through a series of steps of explant disinfection, cluster bud induction, strong seedlings, in vitro preservation, and rooting, effectively solves the problem of the distribution area of ​​Rhododendron grandiflora in Guizhou. Narrow and difficult to introduce and domesticate, the number of plants is small, and it is adjacent to the status quo of endangered species, so as to achieve the purpose of species protection. The tissue culture rapid propagation method provided by the present invention has a multiplication coefficient of 8-10 within one month, a rooting rate of 95%, and a transplanting survival rate of 95%-98%, which greatly improves the reproduction coefficient of Rhododendron grandiflora in Guizhou , The in vitro preservation can reach more than 240d, which provides technical support for the introduction and domestication of this species, in vitro preservation, and seedling expansion and return to planting.

The acer yangbiense is regarded as one of the rarest or endangered species in the world, so a nursing method thereof and a population quantity enlarging method thereof are the basic methods for saving the species. The selected plants of the acer yangbiense which are cultured by the invention are researched in the following aspects of conservation biology, environmental biology, and the like, to provide material foundation, scientific basis and technical support for leading the acer yangbiense back to nature and realizing species reconstruction. The invention comprises the steps of breaking the dormancy of the acer yangbiense seedlings; firstly, sterilizing with 0.05% potassium permanganate solution for 10 minutes; secondly, putting the seedlings under the environment of 5 DEG.C after seeddressing with 80% Zineb wettable powder; putting the seedlings under the environment of 15 DEG.C-20 DEG.C for eight hours twice a moth; germinating; processing for 4 months; and seeding into a growing media with turf: perlite: peat: red earth: organic fertilizer=1:1:1:1:1 after the seedlings germinate.

The invention discloses a method for tissue culture, rapid propagation and cultivation of P. chinensis P. hydrangea. Capsule collection and preservation → disinfection → induction of protocorms → proliferation and differentiation cultivation → strong seedling and rooting cultivation → seedling hardening and transplanting → transplanting Greenhouse planting → pest control and other steps to breed seedlings efficiently and quickly. The tissue culture rapid propagation technology system established by the present invention does not need to invest too much infrastructure in the whole process. A capsule that has been pollinated successfully for 300 days can obtain 2000-3000 complete plants after 18 months. Using a specific medium, the seeds germinate The rate is more than 80%, the input cost is low, the operation is simple, easy to master, and the success rate is high. It has realized the artificial mass breeding of Hengduan Yazhi Paphiopedilum, which is of great importance to the regeneration and sustainable utilization of endangered plant resources and the protection of germplasm resources. meaning.

The invention discloses a method for producing seeds of ammopiptanthus nanus for ex-situ conservation in a desert region. The flower bud differentiation stage of ammopiptanthus nanus is managed. The method comprises the specific steps of fertilization, irrigation, prevention and control of harmful creatures and overwintering management and protection; flowering period management of ammopiptanthusnanus comprises the specific steps that prevention of low-temperature freezing damage, prevention of high wind, fertilization in spring, irrigation and artificial supplementary pollination; fruiting period management of ammopiptanthus nanus comprises the specific steps of topdressing, irrigation, prevention and control of harmful creatures and seed harvesting; through artificial fine breeding andmanagement, ammopiptanthus nanus can bloom in quantity, lots of seeds with vitality can be produced, the difficult problem of dysgenesis in ex-situ conservation is solved, and the method has importantsignificance on germplasm resource conservation and population enlargement and reproduction of a rare and endangered plant ammopiptanthus nanus.

The invention provides a development method of an EST-SSR molecular marker of an endangered plant sinocalycanthus chinensis. The development method mainly comprises the following steps: identifying and screening an EST sequence and an SSR site of the sinocalycanthus chinensis, thus obtaining an EST-SSR molecular marker primer sequence. According to the development method provided by the invention, 200 sequences containing different repetitive units are searched from data of a sinocalycanthus chinensis transcriptome, and software analysis and molecular marker screening are combined to obtain 23 EST-SSR markers with abundant polymorphism. The EST-SSR marker can be applied to genetic diversity analysis, germplasm identification, variation and evolution researches, molecular marker assisted breeding and the like of different species and different species groups of the endangered plant sinocalycanthus chinensis. The EST-SSR marker has good repeatability and high reliability and is a very effective EST-SSR molecular marker.

The invention discloses a method for quickly cultivating pine seedlings through immature embryos, aiming to solve the problems of small populations of pine and low genetic diversity caused by cutting propagation in production. The present invention uses the immature cones of the cutting plants of Pingyao Town, Yuhang District, Zhejiang Province as starting materials, strips off the endosperm and immature embryos after surface disinfection, and quickly cultivates the sexual offspring of the water pine plants through the embryo culture technology. A large number of cork pine seedlings can be obtained within a short period of time. The invention has the advantages of using the immature embryo with endosperm of the water pine to obtain the sexual offspring population of the water pine after 30 days of culture, improving the current situation that the water pine is mainly propagated by cuttings, and accelerating the cultivation speed of the water pine seedlings. The method is highly operable, simple and efficient, and has a low pollution rate, which is of great significance to the protection of rare and endangered plants in the world and the rapid cultivation of seedlings.

The invention relates to a method of artificial fast breeding for spun gold suspended gourd, with the detail steps in below: 1) pretreating the explant, 2) sterilizing the explant, 3) inoculating, 4) inducing culturing, 5) differentiation culture for callus, 6) enrichment culture, 7) strengthening sprout culture, 8) root-growing culture, 9) sprouting training and replanting, 10) sprout bed management, 11) bedding in earth the culture sprout, 12) shearing the cuttage ear, 13) treating the cuttage ear with medical agent, 14) cuttage, 15) cuttage sprout bed management. The benefit of this invention is that:: by making use of the advantage of plant tissue culture technique to breed large quantity of spun gold suspended gourd tissue culture sprout in tube with a high speed, the monthly breeding rate is 3-5 times, the yearly tissue culture sprout producing ability is more than ten thousand per square meter, and it solves the problem of spun gold suspended gourd resource shortage. The provided artificial fast breeding method uses less explant material, which can effectively save rare endangered plant species, and the mass production can be carried out which can provide seed foundation for the further development of spun gold suspended gourd product.

The invention discloses a method for artificially planting mangosteen, comprising the following steps: (1) soil preparation and transformation; (2) cultivating tissue-cultured seedlings of mangosteen; (3) transplanting; and (4) field management. The present invention realizes the large-scale planting of Manglia argensis by cultivating tissue-cultured seedlings of Manglia argentea, and overcomes the difficult problem that Manglia argentea is not easy to survive in artificial planting as an endangered plant. As high as 85% or more, it has extremely important value and significance for protecting the endangered plant ash magnolia in our country.

The invention provides a method for the rapid and efficient germination and seedling growth of the rare and endangered plant Akane baoshan, comprising the following steps: (1) collection of fruit and seeds: collecting Akane baoshan fruit with gray-green exocarp; peeling off the exocarp; taking out the seeds Baoshanqian seeds with light brown or dark brown leopard-like membranous skin and slightly bony endosperm or bony endosperm; (2) seed disinfection; (3) water moss germination: spread a layer of water moss, and spread fresh seeds on the water moss (4) Preparation of transplanting substrate; (5) Transplanting: root directly in the sphagnum, the radicle grows to 3±0.3cm, the radicle has root hairs, and the radicle axis is green When transplanting to the seedling-raising container, the roots with root hairs are planted in the seedling-raising matrix, the thick endosperm of the radicle rachis and the cotyledons are exposed outside the seedling-raising matrix, and 800 times of carbendazim or chlorothalonil is poured; (6) management. The invention greatly improves the germination rate and seedling rate of Baoshan Qian seeds, shortens the seedling raising time, makes the seedlings germinate neatly, and is easy to manage.

The invention belongs to the technical field of agriculture and forestry seedling tissue culture, and particularly relates to a tissue culture method of parakmeria omeiensis cheng. The method comprises the following steps of: disinfecting the epicormic branches of the parakmeria omeiensis cheng; and inducing, breeding and rooting by common stem tips to obtain regenerated seedlings. The method has the advantages that plants which are easily available are taken as female parent, and the stem tips are explant material; the disinfecting treatment method is perfected; the problem that seeds are difficult to obtain effectively in the existing sexual propagation is solved; and a tissue culture system of the parakmeria omeiensis cheng is successfully set up, and the threatened plant-parakmeria omeiensis cheng in China can be saved.