Tissue culture method of parakmeria omeiensis cheng

A pseudomonophyte magnolia, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem that it is difficult to obtain reproducible seeds

Inactive Publication Date: 2014-06-11
四川省自然资源科学研究院 +1
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  • Application Information

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Problems solved by technology

Emei pseudo-unisexual magnolia is mainly distributed in evergreen broad-leaved forests. Its aggregate fruit is obovate, 3-4 cm long, its seeds are obovate, 6-8 mm in diameter, and the outer testa is reddish brown, which is easy to attract birds. species foraging; thus difficult to obtain fertile seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] Take the twigs of Emei pseudoparthenosexual magnolia and soak them in 0.1% washing powder water for 30 minutes, then rinse them with tap water for 1 hour and set aside.

[0012] Under aseptic conditions, the twigs of Pseudomonas magnolia Emei were sterilized with 70% alcohol for 30 seconds, and 0.1% HgCl 2 Treat for 10-15 min, rinse with sterile water 5-6 times, take 1 cm long common shoot tips as explants, and inoculate induction medium.

[0013] The induction medium was MS-based medium, supplemented with 6-BA 2.0 mg / L, IAA 0.02 mg / L, KT 1.5 mg / L, sucrose 3%, agar 0.5%, pH 5.8, culture temperature 27±2°C, Illuminated for 12 hours, light intensity 2000Lx, cultured for 40-45 days to grow into shoots of about 3 cm, cut the germinated shoots, and transferred to the differentiation medium.

[0014] The differentiation medium is based on MS, supplemented with 6-BA 0.5 mg / L, NAA 0.02 mg / L, sucrose 3%, agar 0.5%, pH 5.8, culture temperature 27±2°C, light 12h, light intensity ...

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Abstract

The invention belongs to the technical field of agriculture and forestry seedling tissue culture, and particularly relates to a tissue culture method of parakmeria omeiensis cheng. The method comprises the following steps of: disinfecting the epicormic branches of the parakmeria omeiensis cheng; and inducing, breeding and rooting by common stem tips to obtain regenerated seedlings. The method has the advantages that plants which are easily available are taken as female parent, and the stem tips are explant material; the disinfecting treatment method is perfected; the problem that seeds are difficult to obtain effectively in the existing sexual propagation is solved; and a tissue culture system of the parakmeria omeiensis cheng is successfully set up, and the threatened plant-parakmeria omeiensis cheng in China can be saved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of agricultural and forestry seedlings, in particular to a method for tissue culture of Emei pseudoparthenophyte magnolia. Background technique [0002] Emei Pseudo-Phenophyte Magnolia is a species of Pseudo-Phenophyte Magnolia in the family Magnoliaceae. It is a unique evergreen plant in Mount Emei, Sichuan. Due to deforestation and destruction of vegetation, there are very few plants in the original place, and strict protection measures are urgently needed. Emei pseudomonosexual magnolia is mainly distributed in evergreen broad-leaved forests, its aggregate fruit is obovate, 3-4 cm long, its seeds are obovate, 6-8 mm in diameter, and the outer testa is reddish brown, which is easy to attract birds species foraging; therefore it is difficult to obtain fertile seeds. It is imminent to find a safe breeding method and save the endangered plant Pseudomonas magnolia Emei. Contents of the in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 秦小波
Owner 四川省自然资源科学研究院
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