A method for in vitro preservation of Primula chinensis in eastern Sichuan

Abstract

The invention discloses a method for in vitro preservation of Primula chinensis in eastern Chuandong. The method is suitable for in vitro preservation of the endangered plant Primula chinensis in eastern Sichuan, and belongs to the field of plant biotechnology. The present invention uses the non-diseased leaves of Primula dengtai in eastern Sichuan as explants, and through a series of steps such as callus induction and differentiation, in vitro preservation, recovery of growth, rooting culture, etc., greatly improves the reproduction of Primula in eastern Sichuan At the same time, the in vitro preservation time can be as long as 210 days or more, so that the endangered plant Primula chrysalis can obtain excellent breeding technology and continuous provenance, and avoid it from being extremely endangered or extinct. Because the plant is already in an endangered state in the wild and the number is very small, the present invention uses its leaves as explants, which greatly reduces the damage to the mother plant, and the reproduction speed is fast, the reproduction coefficient in one month is 8-15, and the root The transplanting survival rate is about 90%-95%, and the transplanting survival rate is about 93%-95%, which greatly improves the reproduction speed of Primula dengtai in eastern Sichuan, and solves the endangered status of its few seeds, few wild plants, and difficult reproduction.

Description

technical field [0001] The invention relates to a method for plant tissue culture in the field of biotechnology, in particular to a method for in vitro preservation of the endangered plant Primula chuandongensis. Background technique [0002] Primula mallophylla (Primula mallophylla) belongs to Primulaceae Primulaceae. The type specimen of Primula chinensis in eastern Sichuan was collected for the first time by the British P. Farges in the Dabashan area of ​​Chengkou District (now Chengkou County, Chongqing City) at the end of the 19th century, and was stored in the Herbarium of Paris, France. The species name is preserved in the herbaria of Kew Gardens and Edinburgh Botanic Gardens. In 1916, botanist I.B.Balfour identified the new species of "East Sichuan Lighthouse Primula" based on these specimen materials. Since no botanists have collected this plant since then, it was included in the "Extinct (EX)" category in the "China Red List" published in 2004. In 2006, Mr. Zhan...

AI Technical Summary

Problems solved by technology

Using the method of subculture for germplasm preservation, the outstanding problem is that the herbaceous plant subculture cycle is short, and frequent subculture transfers are required to preserve the species

And as the number of subcultures increases, the materials will vary to varying degrees. At the same time, more and more materials will be multiplied, resulting in rising manpower and material costs.