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Method for increasing germination rate of seeds of threatened plant gymnadenia cono-psea

A technology of Bergamot ginseng and seeds, applied in the biological field, can solve the problems of low germination rate and achieve the effect of improving seed germination rate, good effect and low cost

Inactive Publication Date: 2016-03-23
ZHEJIANG TOP MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This study also conducted the non-symbiotic germination research on Bergamot ginseng seeds for the first time, and the seed germination rate can reach 20%, which is already a great breakthrough, but the germination rate is still very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for improving the germination rate of endangered plant Bergamot bergamot seeds, characterized in that the steps are as follows:

[0028] (1) Preprocessing:

[0029] Take moderately mature seeds of Bergamot ginseng, yellow seeds that are not cracked and easy to peel off from the placenta, rinse with running water for 30 minutes, then add to the pretreatment solution, place in a constant temperature ultrasonic generator (power: 25KHz), and treat at a constant temperature of 25°C for 3 minutes ;

[0030] The formula of the pretreatment liquid is: malic acid 1g / L, laccase 0.8g / L (enzyme activity is 5000u / g), glycine 2g / L, and the rest is pure water;

[0031] (2) Germination medium culture:

[0032] After pretreatment, use neutral detergent to clean, then rinse with distilled water for 5 times, transfer it to a sterile bottle under aseptic conditions; rinse with sterile water for 3 times, rinse with 75% alcohol Disinfect for 20 seconds, rinse with sterile water f...

Embodiment 2

[0041] A method for improving the germination rate of endangered plant Bergamot bergamot seeds, characterized in that the steps are as follows:

[0042] (1) Preprocessing:

[0043] Take moderately mature seeds of Bergamot ginseng, yellow seeds that are not cracked and easy to peel off from the placenta, rinse with running water for 30 minutes, then add to the pretreatment solution, place in a constant temperature ultrasonic generator (power: 25KHz), and treat at a constant temperature of 28°C for 3 minutes ;

[0044] The formula of the pretreatment liquid is: malic acid 1g / L, laccase 0.8g / L (enzyme activity is 5000u / g), glycine 2g / L, and the rest is pure water;

[0045] (2) Germination medium culture:

[0046] After pretreatment, use neutral detergent to clean, then rinse with distilled water for 5 times, transfer it to a sterile bottle under aseptic conditions; rinse with sterile water for 3 times, rinse with 75% alcohol Disinfect for 20 seconds, rinse with sterile water f...

Embodiment 3

[0055] A method for improving the germination rate of endangered plant Bergamot bergamot seeds, characterized in that the steps are as follows:

[0056] (1) Preprocessing:

[0057] Take moderately mature seeds of Bergamot ginseng, yellow seeds that are not cracked and easy to peel off from the placenta, rinse with running water for 30 minutes, then add to the pretreatment solution, place in a constant temperature ultrasonic generator (power: 25KHz), and treat at a constant temperature of 26°C for 3 minutes ;

[0058] The formula of the pretreatment liquid is: malic acid 1g / L, laccase 0.8g / L (enzyme activity is 5000u / g), glycine 2g / L, and the rest is pure water;

[0059] (2) Germination medium culture:

[0060] After pretreatment, use neutral detergent to clean, then rinse with distilled water for 5 times, transfer it to a sterile bottle under aseptic conditions; rinse with sterile water for 3 times, rinse with 75% alcohol Disinfect for 20 seconds, rinse with sterile water f...

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Abstract

The invention discloses a method for increasing the germination rate of seeds of threatened plant gymnadenia cono-psea. The method includes the steps of firstly, conducting pretreatment; secondly, conducting cultivating through a germination culture medium, wherein after pretreatment, sterilization treatment is conducted, capsules are cut longitudinally through a sterile surgical knife blade, seeds are picked out and dispersed in the germination culture medium, and inoculated seeds are cultured for 5 days under the dark condition at the temperature of 18 DEG C, continue to be cultured for 1 day under the dark condition at the temperature of 25 DEG C and then continue to be cultured under the dispersed light conditions of the luminance cycle of 12 h.d<-1> and the luminance intensity of 3.2 micromole.m<-2>.s<-1>. The germination culture medium is prepared from 11.8 g / L of cane sugar, 3.9 g / L of agar, 0.5 g / L of compound traditional Chinese medicine extracting solution, 8 g / L of coconut juice and 0.1 mg / L of 6-BA with MS as the basic culture medium and has the pH value of 6.5.

Description

technical field [0001] The invention relates to a method for improving the germination rate of endangered plant bergamot ginseng seeds, which belongs to the field of biology. Background technique [0002] Orchidaceae Ginseng is a perennial herbaceous plant, there are 10 species in the world, and 5 species in my country, including Southwest Ginseng, Ginseng, Short-range Ginseng, Emei Ginseng and Angular-growth Ginseng. Bergamot ginseng has been included in many Tibetan medicine classics because of its considerable medicinal value. [0003] The seeds of orchids are extremely small (each seed is 1-2mm long and 0.1-0.2mm in diameter), and because the embryo is not fully developed and lacks endosperm to supply nutrients, it needs to live symbiotically with the fungi in the habitat under natural conditions. Germination, the natural germination rate is extremely low. Orchids can be divided into aerial orchids and terrestrial orchids according to their growth environment. In order...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 谢再法姚凤鸣郭锋燕
Owner ZHEJIANG TOP MEDICINE
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