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Tissue Culture Breeding Method of Manglisia argentifolius

A technology of ash wood and tissue culture, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as unfavorable large-scale engineering seedling cultivation of ash wood lotus

Active Publication Date: 2021-08-10
XIANGZHOU COUNTY INST OF SCI & TECHN INFORMATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method breaks through the technical problem of asexual reproduction of Manglia argentifolius, it is unfavorable for realizing large-scale engineering seedling cultivation

Method used

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  • Tissue Culture Breeding Method of Manglisia argentifolius
  • Tissue Culture Breeding Method of Manglisia argentifolius
  • Tissue Culture Breeding Method of Manglisia argentifolius

Examples

Experimental program
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Embodiment 1

[0019] (1) Treatment of explants: Take 8-year-old ash magnolia tree as the female parent, select young buds of the year without pests and diseases as explants, and perform the following series of treatments: first rinse with pure water for 3 to 4 times , after blotted with absorbent paper, soak in 0.5% potassium permanganate solution for 20-25 minutes, rinse with sterile water for 2-3 times, blot with absorbent paper, and cut into 3-3.5cm pieces with sterilized dissecting scissors. A small section of , spare.

[0020] (2) Cultivation in induction medium: Put the cut pieces on the induction medium for cultivation: first culture in total darkness at 23-25°C for 20-22 days, and then place in light for 15-17 hours per day, with a light intensity of 1200~1500lx, cultivated at a temperature of 26~28°C to induce sprouting; the induction medium formula is: MS+6-BA1~1.5mg / L+NAA0.5~0.75mg / L+ sucrose 3g / L + agar 4g / L, the pH value is 5.8-6.0. The present invention is as follows to the ...

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Abstract

The invention discloses a method for tissue culture and breeding of mangosteen, which is characterized in that it comprises the following steps: (1) explant treatment; (2) induction medium culture; (3) proliferation culture; (4) root culture (5) seedling hardening. The present invention realizes the tissue culture seedling cultivation of Manglia argentifolius. Compared with the traditional seed propagation, the seedling cultivation of tissue culture is more conducive to realizing the engineering seedling raising of Manglia argentina. important value and significance.

Description

technical field [0001] The invention belongs to the field of crop breeding, and in particular relates to a method for tissue culture and breeding of mangosteen. Background technique [0002] Ash magnolia ( Manglietia glauca Blume ) is a single-species plant unique to my country in the Magnoliaceae, a national first-class protected and endangered plant. It has found a link between Magnolia and Magnolia in the family Magnoliaceae, which plays an extremely important role in the study of the evolution of the system of Magnoliaceae and the origin of angiosperms. As an endangered plant, magnolia has great research value. As a garden tree, it has the functions of greening, beautifying and purifying: magnolia is evergreen in four seasons, with luxuriant branches and leaves, fast growth and strong adaptability; The shape is straight, the tree shape is beautiful, the flowers are many and the flowering period is long, the flowers are large and white, and can emit a fragrance. Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫晓君雷军强韦颖婷李柳孙敬华覃鸫鸰
Owner XIANGZHOU COUNTY INST OF SCI & TECHN INFORMATION
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