Tissue culture cultivating method for manglietia glauca

A technology of ash wood and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the unfavorable problems of large-scale engineering seedling cultivation of ash magnolia

Active Publication Date: 2018-08-10
XIANGZHOU COUNTY INST OF SCI & TECHN INFORMATION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method breaks through the technical problem of asexual reproduction of Manglia argentifolius, it is unfavorable for realizing large-scale engineering seedling cultivation

Method used

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  • Tissue culture cultivating method for manglietia glauca
  • Tissue culture cultivating method for manglietia glauca
  • Tissue culture cultivating method for manglietia glauca

Examples

Experimental program
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Embodiment 1

[0019] (1) Explant treatment: take 8-year-old ash magnolia tree as the female parent, select the young buds of the year without pests and diseases as explants, and perform the following series of treatments: first rinse with pure water 3 to 4 times , after blotted with absorbent paper, soak in 0.5% potassium permanganate solution for 20-25 minutes, rinse with sterile water 2-3 times, blot with absorbent paper, and cut into 3-3.5cm pieces with sterilized dissecting scissors. A small section of , spare.

[0020] (2) Cultivation in induction medium: Put the cut pieces on the induction medium for cultivation: first culture in total darkness at 23-25°C for 20-22 days, and then place in light for 15-17 hours per day, with a light intensity of 1200~1500lx, cultivated at a temperature of 26~28°C to induce sprouting; the induction medium formula is: MS+6-BA1~1.5mg / L+NAA0.5~0.75mg / L+ sucrose 3g / L + agar 4g / L, the pH value is 5.8-6.0. The present invention is as follows to the optimize...

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Abstract

The invention discloses a tissue culture cultivating method for manglietia glauca. The method is characterized by comprising the following steps of (1) explant treatment; (2) culture by an inducing culture medium; (3) proliferation culture; (4) rooting culture; (5) seedling hardening. The method achieves the tissue culture for growing manglietia glauca seedlings, compared with the traditional seedpropagation, the tissue culture for growing seedlings is more beneficial to achieving engineering seedling growth of the manglietia glauca, the survival rate of the tissue culture seedlings is high,and the method has more important value and significance in protecting the endangered manglietia glauca.

Description

technical field [0001] The invention belongs to the field of crop breeding, and in particular relates to a method for tissue culture and breeding of mangosteen. Background technique [0002] Magnoliaceae glanca Blume (Magnoliaceae glanca Blume) is a single-species plant unique to my country in the Magnoliaceae family. It is a national first-class protected and endangered plant. It has found a link between Magnolia and Magnolia in the family Magnoliaceae, which plays an extremely important role in the study of the evolution of the system of Magnoliaceae and the origin of angiosperms. As an endangered plant, magnolia has great research value. As a garden tree, it has the functions of greening, beautifying and purifying: magnolia is evergreen in four seasons, with luxuriant branches and leaves, fast growth and strong adaptability; The shape is straight, the tree shape is beautiful, the flowers are many and the flowering period is long, the flowers are large and white, and can ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫晓君雷军强韦颖婷李柳孙敬华覃鸫鸰
Owner XIANGZHOU COUNTY INST OF SCI & TECHN INFORMATION
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