Silverback Artocarpus in vitro breeding method

A technology of in vitro propagation and explants, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that the in vitro propagation method of laurel wood has not been reported.

Inactive Publication Date: 2004-12-29
DONGGUAN FORESTRY INST
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  • Abstract
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Problems solved by technology

[0008] The in vitro propagation method of Osmant...

Method used

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  • Silverback Artocarpus in vitro breeding method

Examples

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Embodiment 1

[0030] The in vitro propagation method of laurel wood consists of the following steps:

[0031] (1), the acquisition of sterile explants;

[0032] Use the stem tip, stem segment, and leaf of the excellent seedling of the mother plant as explants; rinse the stem tip and stem segment with tap water, treat with 70% alcohol for 20 minutes, rinse 5 times with sterile water, and use 0.1% mercury chloride Treat for 10 minutes, wash with aseptic water 4 times, cut the shoot tips into 1.5cm long pieces and inoculate them into the induction medium. The success rate of mother plant stems is 3%, and the success rate of annual stems is 5%. Within 7 days of material inoculation, some incisions have light brown substances secreted and have a water-soaked shape. Such materials will die slowly, and some will turn green, and the incisions will swell, but there is no callus. After 25 days, small buds appeared. After the leaves were rinsed with tap water, they were treated with 70% alcohol for...

Embodiment 2

[0046] The method is the same as in Example 1, but the explants in the step (1) are the stem tips, stem segments, and leaves of the seedlings obtained by aseptic sowing. This method is faster than Example 1.

[0047] The above method of aseptic sowing is as follows: Immediately after the seeds are harvested, they are rinsed with tap water, dried, treated with 70% alcohol for 10-15 seconds the next day, rinsed with sterile water for 3-5 times, and then sterilized with 0.1% mercuric chloride for 3 minutes. Rinse with sterile water five times and inoculate into MS medium, place in an incubator for dark cultivation, keep the temperature at 28°C, and cultivate for 30 days. After 30 days, a complete plant with roots, stems and leaves will grow.

[0048] In August 2002, 26 seeds were collected. Immediately after the seeds were collected, they were rinsed with tap water, dried, treated with 70% alcohol for 12 seconds the next day, rinsed with sterile water for 4 times, and then steril...

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Abstract

The excised white laurel propagating process consists of the following steps: obtaining aseptic explant, inducing bud, proliferating bud, inducing and culturing root, and transplanting test seedling. The present invention is excised tissue culture and fast propagation technology for providing excellent tissue cultured seedlings of white laurel as one kind of endangered plant species.

Description

Technical field: [0001] The invention relates to an in vitro propagation method of plants, in particular to an in vitro propagation method of laurel wood. Background technique: [0002] In 1994, my country successively promulgated the "China Biodiversity Action Plan" and "China's 21st Century Agenda", which pointed out that "15%-20% of China's animal and plant species are threatened, which is higher than the world's 10%-15% Level". my country's plant resources are very rich, with about 30,000 species. Based on this, it is estimated that about 4,500-6,000 species of plants in my country are endangered or threatened. Some of these populations have been reported to contain only one or a few strains. Since the 1980s, my country began to establish the "National Rare and Endangered Plant Germplasm Gene Resource Bank and Database", and published the "Chinese Plant Red Book" (Volume 1) in 1991. The protection and preservation of endangered rare plants mainly include ex situ preser...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘颂颂叶永昌招晓东
Owner DONGGUAN FORESTRY INST
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