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Method for controlling Codonopsis pilosula yield and metabolite accumulation by utilizing bacteriabeneficial beneficial bacteria

A metabolite and beneficial technology, applied in the field of microbiology and plant physiology, can solve the problems of limited quantity of commodities, scattered resources and output, contradiction between supply and demand in the Codonopsis ginseng market, and achieve non-pathogenicity and strong stress resistance, simple treatment methods, rich effect

Inactive Publication Date: 2014-02-05
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the national Codonopsis ginseng wild resource reserve is about 15,800 tons, but the resources are scattered and the output is declining year by year. The number of commodities that can be provided is extremely limited, and the contradiction between supply and demand in the Codonopsis ginseng market is extremely prominent (Liu Dejun et al. 2001)

Method used

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  • Method for controlling Codonopsis pilosula yield and metabolite accumulation by utilizing bacteriabeneficial beneficial bacteria
  • Method for controlling Codonopsis pilosula yield and metabolite accumulation by utilizing bacteriabeneficial beneficial bacteria
  • Method for controlling Codonopsis pilosula yield and metabolite accumulation by utilizing bacteriabeneficial beneficial bacteria

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: seedling cultivation

[0023] The Codonopsis pilosula seeds are germinated, and after the seeds germinate, they are transplanted into a hole tray with vermiculite, watered with 1 / 2 Hoagland nutrient solution, and cultivated in a greenhouse; the day and night temperature of the greenhouse is 26-30°C, and the light is 12- 14h / d, the light intensity is 800-1000μmol / m2·s, and the relative humidity is 60-80%.

Embodiment 2

[0024] Embodiment 2: seedling transplanting

[0025] After the seedlings have grown for 28-32 days, transplant them into plastic flowerpots with a diameter of 18-22cm and a depth of 28-30cm. 3d, then potting, weighing, and controlling the amount of watering to 60% of the maximum water holding capacity in the field;

Embodiment 3

[0026] Embodiment 3: Bacterial liquid preparation

[0027] Preparation of LB liquid medium: Prepare according to the ratio of sodium chloride 5g / L, tryptone 10g / L and yeast powder 5g / L, adjust the pH to 6.8-7.2, sterilize at 121-126°C and wait for cooling. Add 50-100 μL of GB03 bacterial liquid to 100 mL of LB liquid medium, cultivate overnight on a constant temperature culture shaker at a speed of 180 r / min and a temperature of 25-28 ° C, and use a nucleic acid detector to measure the concentration of the bacterial liquid as OD 600 =0.8-1.0, take it out for use;

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Abstract

The invention relates to a method for controlling Codonopsis pilosula yield and metabolite accumulation by utilizing bacteriabeneficial beneficial bacteria. The method is characterized by comprising the following steps of seedling cultivation: germinating Codonopsis pilosula seeds, transplanting the germinated Codonopsis pilosula seeds to hole trays filled with vermiculite, watering the germinated Codonopsis pilosula seeds by 1 / 2 Hoagland nutrient solution and cultivating the germinated Codonopsis pilosula seeds in a greenhouse, wherein in the greenhouse, the diurnal temperature is 26-30 DEG C, illumination is performed for 12-14 hours per day with light intensity of 800-1000mumol / m<2>*s, and the relative humidity is 60-80%. The plant bacteriabeneficial beneficial microorganism promotes plants to absorb nutrient substances and relieve plant diseases, therefore, growth conditions of the plants are improved, root development and growth of the Codonopsis pilosula are promoted, and then contents of some metabolic substances in the Codonopsis pilosula can be changed. Bacillus subtilis has the advantages of being free of toxicity and harm to people and livestock, free of pollution to environment, non-pathogenic and strong in stress resistance, and can produce a variety of growth hormone-like chemicals and rich antimicrobial substances, promote plant growth and control accumulation of plant metabolite.

Description

technical field [0001] The invention belongs to the field of microbiology and plant physiology, and relates to a method for using rhizosphere beneficial bacteria Bacillus subtilis GB03 to regulate the development and growth of the root system of Codonopsis pilosula, and regulate the accumulation of active components and metabolites, thereby increasing the yield and quality of Codonopsis pilosula. Background technique [0002] Bacillus subtilis has the characteristics of non-toxic and harmless to humans and animals, no pollution to the environment, non-pathogenicity and strong stress resistance. It can produce a variety of growth hormone substances and rich antibacterial substances to promote plant growth. Improve plant growth and development, increase plant resistance to salt stress and other adversities, thereby improving plant disease resistance. [0003] In recent years, Codonopsis pilosula has made new progress in the treatment of coronary heart disease, hyperlipidemia, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
Inventor 张金林吴永娜王锁民吕昕培李静韩庆庆包爱科
Owner LANZHOU UNIVERSITY
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