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A method for rapidly cultivating Chinese pine seedlings through immature embryos

An immature embryo and immature technology, applied in the field of rapid cultivation of Chinese pine seedlings, can solve the problems of low survival rate and low germination rate, and achieve the effect of low pollution rate and strong operability

Active Publication Date: 2022-05-06
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Water pine is a tree species that likes moisture. After the seeds mature, they often fall into the swamp. The germination rate is very low or none, and the seedlings grow in water for a long time, and the survival rate is not high.

Method used

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  • A method for rapidly cultivating Chinese pine seedlings through immature embryos
  • A method for rapidly cultivating Chinese pine seedlings through immature embryos
  • A method for rapidly cultivating Chinese pine seedlings through immature embryos

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Materials: In this example, immature embryos and endosperms of Chinese pine were used as explants for culture.

[0027] Step (1): Collect materials

[0028] The immature cones of Chinese pine are collected from late August to late October, and the color is green. The surface scales and bract scales of this green immature fruit are closely arranged. It can be placed in a sealed bag and stored at a low temperature of 4°C for later use.

[0029] Step (2): Seed Surface Sterilization

[0030] Separate the seed scales and seeds with tweezers, wash the seeds under running water for 3 hours, treat them with 70% ethanol for 30 seconds on an ultra-clean workbench, and rinse them with sterile water for 3 to 5 times. After treating with 0.1wt% mercuric chloride for 10 minutes, rinse with sterile water for 3 to 5 times and dry the surface moisture with sterile filter paper.

[0031] Step (3): Immature embryo isolation and inoculation

[0032] Place the rinsed seeds in a petri di...

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Abstract

The invention discloses a method for quickly cultivating pine seedlings through immature embryos, aiming to solve the problems of small populations of pine and low genetic diversity caused by cutting propagation in production. The present invention uses the immature cones of the cutting plants of Pingyao Town, Yuhang District, Zhejiang Province as starting materials, strips off the endosperm and immature embryos after surface disinfection, and quickly cultivates the sexual offspring of the water pine plants through the embryo culture technology. A large number of cork pine seedlings can be obtained within a short period of time. The invention has the advantages of using the immature embryo with endosperm of the water pine to obtain the sexual offspring population of the water pine after 30 days of culture, improving the current situation that the water pine is mainly propagated by cuttings, and accelerating the cultivation speed of the water pine seedlings. The method is highly operable, simple and efficient, and has a low pollution rate, which is of great significance to the protection of rare and endangered plants in the world and the rapid cultivation of seedlings.

Description

technical field [0001] The invention relates to a method for rapidly cultivating Chinese pine seedlings through immature embryos, so as to achieve the purposes of protecting rare and endangered species and rapidly propagating seedlings. Background technique [0002] Water pine, Latin scientific name: Glyptostrobus pensilis; [0003] Alias: water lotus, water emperor pine, metasequoia pine, water pole, long cypress; [0004] Family and genus: Cypress cypress subfamily Cypress; [0005] Origin and distribution: There is only one species of Chinese pine in the genus, which was widely distributed in the northern hemisphere before the Tertiary period. After the Quaternary glaciers, the water pine plants became extinct in Europe and America, and are now only distributed in my country and Vietnam. Water pine grows in latitude 13°09′ (Vietnam)-37°33′ (Shandong Yantai), longitude 102°42′ (Yunming Kunming)-121°31′ (Chinese Taipei), and altitude 32-1980m. Distributed in the Pearl Ri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 徐礼根陈利萍马思妤帅辉刘柯王挺进李玉卓曹丽雯
Owner ZHEJIANG UNIV
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