Method for quickly cultivating glyptostrobus seedling through immature embryo
An immature embryo and immature technology, applied in the field of rapid cultivation of water pine seedlings, can solve the problems of low germination rate and low survival rate, and achieve the effect of strong operability and low pollution rate
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[0026] Materials: In this example, immature embryos and endosperms of Chinese pine were used as explants for culture.
[0027] Step (1): Collect materials
[0028] The immature cones of Chinese pine are collected from late August to late October, and the color is green. The surface scales and bract scales of this green immature fruit are closely arranged. It can be placed in a sealed bag and stored at a low temperature of 4°C for later use.
[0029] Step (2): Seed Surface Sterilization
[0030] Separate the seed scales and seeds with tweezers, wash the seeds under running water for 3 hours, treat them with 70% ethanol for 30 seconds on an ultra-clean workbench, and rinse them with sterile water for 3 to 5 times. After treating with 0.1wt% mercuric chloride for 10 minutes, rinse with sterile water for 3 to 5 times and dry the surface moisture with sterile filter paper.
[0031] Step (3): Immature embryo isolation and inoculation
[0032] Place the rinsed seeds in a petri di...
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