Method for quickly cultivating glyptostrobus seedling through immature embryo

An immature embryo and immature technology, applied in the field of rapid cultivation of water pine seedlings, can solve the problems of low germination rate and low survival rate, and achieve the effect of strong operability and low pollution rate

Active Publication Date: 2019-04-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Water pine is a tree species that likes moisture. After the seeds mature, they often fall into the swamp. The germination rate is very low or none, and the seedlings grow in water for a long time, and the survival rate is not high.

Method used

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  • Method for quickly cultivating glyptostrobus seedling through immature embryo
  • Method for quickly cultivating glyptostrobus seedling through immature embryo
  • Method for quickly cultivating glyptostrobus seedling through immature embryo

Examples

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Embodiment 1

[0026] Materials: In this example, immature embryos and endosperms of Chinese pine were used as explants for culture.

[0027] Step (1): Collect materials

[0028] The immature cones of Chinese pine are collected from late August to late October, and the color is green. The surface scales and bract scales of this green immature fruit are closely arranged. It can be placed in a sealed bag and stored at a low temperature of 4°C for later use.

[0029] Step (2): Seed Surface Sterilization

[0030] Separate the seed scales and seeds with tweezers, wash the seeds under running water for 3 hours, treat them with 70% ethanol for 30 seconds on an ultra-clean workbench, and rinse them with sterile water for 3 to 5 times. After treating with 0.1wt% mercuric chloride for 10 minutes, rinse with sterile water for 3 to 5 times and dry the surface moisture with sterile filter paper.

[0031] Step (3): Immature embryo isolation and inoculation

[0032] Place the rinsed seeds in a petri di...

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Abstract

The invention discloses a method for quickly cultivating glyptostrobus seedling through immature embryo, and aims at solving the problems that the glyptostrobus sward population is less and the genetic diversity is low due to cutting propagation on the production. The immature strobili of the glyptostrobus cottage plant at the Pingyao Town, Yuhang District, Zhejiang Province is taken as the starting material, the endosperm thereof and the immature embryo are stripped after surface disinfection, the glyptostrobus plant sexual progeny is quickly cultivated through the embryo culture technology,a large number of glyptostrobus seedlings can be acquired within short time. The method disclosed by the invention has the advantages that the glyptostrobus sexual progeny sward is acquired by culturing the immature embryo with endosperm of the glyptostrobus for 30 days, the current status that the glyptostrobus is mainly propagated through the cottage is improved, and the glyptostrobus seedling cultivating speed is accelerated. The method disclosed by the invention is strong in operability, simple and efficient, low in pollution rate, and has significance in protecting the rare or endangeredspecies in the world and quickly cultivating the nursery stock.

Description

technical field [0001] The invention relates to a method for rapidly cultivating Chinese pine seedlings through immature embryos, so as to achieve the purposes of protecting rare and endangered species and rapidly propagating seedlings. Background technique [0002] Water pine, Latin scientific name: Glyptostrobus pensilis; [0003] Alias: water lotus, water emperor pine, metasequoia pine, water pole, long cypress; [0004] Family and genus: Cypress cypress subfamily Cypress; [0005] Origin and distribution: There is only one species of Chinese pine in the genus, which was widely distributed in the northern hemisphere before the Tertiary period. After the Quaternary glaciers, the water pine plants became extinct in Europe and America, and are now only distributed in my country and Vietnam. Water pine grows in latitude 13°09′ (Vietnam)-37°33′ (Shandong Yantai), longitude 102°42′ (Yunming Kunming)-121°31′ (Chinese Taipei), and altitude 32-1980m. Distributed in the Pearl Ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 徐礼根陈利萍马思妤帅辉刘柯王挺进李玉卓曹丽雯
Owner ZHEJIANG UNIV
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