Packed bed type cell bioreactor without stirrer and method for cultivating animal cells
A technology of cell biology and packed bed, which is applied in the direction of biochemical equipment and methods, microorganisms, biochemical instruments, etc., can solve the problems of pollution, cell death, high occurrence rate, etc. small force effect
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[0020] Example 1:
[0021] The packed bed cell bioreactor without stirring device includes: a reaction tank body 110, the reaction tank body is connected to the shaker 50 through an eccentric shaking plate 58, and the shaker shakes the eccentric shaking plate to make the reaction tank body Rotate eccentrically. The upper part of the reaction tank body 110 is a circular truncated shape 100, and the lower part is a cylindrical shape 113. The upper part of the truncated truncated shape is equipped with a guide pipe 130, and the inlet 131 of the guide pipe is close to the reaction tank tank. The inner wall of the body, the outlet 133 of the guide pipe enters the central funnel 160 along the tangential direction of the central funnel, and the reaction tank body has a tank with an air inlet, an air outlet, a liquid inlet, and a liquid outlet. The lid 140 has a packed bed 120 inside the reaction tank.
Example Embodiment
[0022] Example 2:
[0023] In the packed bed cell bioreactor without stirring device according to Example 1, the packed bed includes an upper orifice plate 125 , a lower orifice plate 122 and an empty casing 124 . The inner wall of the inner cylinder 113 of the reaction tank body 110 is connected to the lower orifice plate 122 and the empty casing 124, the upper end of the empty casing 124 is connected to the upper orifice plate 125, the upper orifice plate 125, the empty casing 124 The lower orifice plate 122 constitutes a packed bed 120, and the sheet-like microcarriers 26 are filled between the lower orifice plate and the upper orifice plate. The packed bed 120 is consistent with the centerline of the reaction tank body 110 . The reaction tank has a base 114, the reaction tank 110 and the shaking plate 58 are connected through the base 114, and the reaction tank body 110 and the tank cover 140 are connected by bolts.
[0024] The inner surface of the circular frustum 100 ...
Example Embodiment
[0025] Example 3:
[0026] In the packed bed cell bioreactor without stirring device described in Example 1 or 2, the center funnel has a jacket 164, and the center funnel is equipped with a pressure ring 165 and a pressure ring top wire 166 in the center funnel jacket 164. Press down, the center funnel is arranged at the centerline position of the tank body, and the center funnel jacket passes through figure 2 The 3 central funnel jacket brackets 168 are fixed.
[0027] In the packed-bed cell bioreactor without stirring device, the half cone angle α of the conical surface of the frustum of the upper part of the reaction tank body is 25° to 55°, such as 30° or 35°, 40°, 45° or 50°, which can be determined by experiments according to the required concentration of the nutrient solution and the required circulation speed, generally between 30 and 42° is relatively good, and the outside of the reaction tank is provided with an insulating water jacket 111 .
[0028] In the pack...
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