Method for earthed cultivation and strain production of Pleurotus tuberregium and culture medium

Abstract

The invention relates to a method for earthed cultivation and strain production of Pleurotus tuberregium and a culture medium. The method provided by the invention comprises the following steps of: directly placing sclerotium tissues on a slant culture medium to carry out a strain production; culturing and cultivating by a solid culture medium and then utilizing an earthed cultivation. The method solves the problem that the Pleurotus tuberregium is cultivated by liquid strains or a bag type fruiting method for long time. The slant culture medium is composed of 35-40 parts of corn starch, 15-25 parts of wheat bran, 8-12 parts of cane sugar, 8-12 parts of glucose, 1.8-2.2 parts of monopotassium phosphate, 0.9-1.1 parts of magnesium sulfate, 5-7 parts of yeast powder, 0.008-0.012 part of VB1, 16-20 parts of agar and 800-1200 parts of water. The method provided by the invention is convenient for large-area production, the mycelium is thick and strong, the pollution rate is less than 5%, and the time for obtaining the mycelium only needs 7 days; the husk of the solid culture medium has good air permeability, the pH value can be adjusted by lime, and the solid culture medium satisfies mineral requirements; and the biological conversion rate of the earthed cultivation method can reach more than 80%.

Description

technical field [0001] The invention relates to a soil-covered cultivation of tiger milk mushroom, a strain preparation method and a culture medium, and belongs to the field of biotechnology. Background technique [0002] The culture medium is the nutrient substrate needed for the growth of edible fungi. According to the needs of the test or production, the strains are divided into master clock, original species and production species. The medium must contain all the nutrients for the growth of the bacteria, and each substance has an appropriate concentration ratio and a suitable pH. The materials for preparing the culture medium can be selected according to local conditions, have wide sources, are safe, non-toxic, and low in cost. The culture medium should be strictly sterilized and cultured in a sterile state to achieve the purpose of pure culture of edible fungi. [0003] The slant medium is also a form of solid medium; when it is produced, it should be quantitatively ...

AI Technical Summary

Problems solved by technology

Because the peptone among them is more expensive, and the hyphae produced are sparse, not strong, the yield is not high, and the culture quality is not as good as liquid medium, so it is rarely used for large-scale promotion

At present, the large-scale production of tiger milk mushrooms still relies on liquid species for cultivation for a long time. However, the liquid medium has insufficient testing equipment, and the use of liquid strains for production can easily cause large-scale pollution. Moreover, tiger milk mushrooms The bag-type fruiting method is adopted for the cultivation of the fruit. Since the water in the medium is easy to dry, the cultivation yield cannot be increased by this method, and the biotransformation rate is only 30%.