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In-vitro rapid propagation method of cercidiphyllum japonicum

An isolated and rapid technology, applied in the field of plant tissue culture, can solve the problems of long reproduction cycle, slow reproduction speed, and difficulty in meeting the needs of Lianxiang tree seedlings, and achieve the effect of increasing the multiplication coefficient and long reproduction cycle

Inactive Publication Date: 2015-06-10
刘祖英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the reproduction of Lianxiang tree mainly relies on conventional propagation methods, the breeding cycle is long and the reproduction speed is slow, it is difficult to meet the needs of scientific research and market for Lianxiang tree seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Collection and treatment of explants: The 1-year-old stems with axillary buds collected from the field were washed with tap water to clean the dust on the surface, and then sprayed with 1% dew, and then placed in an artificial climate box at a temperature of 25°C. 70% humidity, 12 hours of light per day, and 1500lx light intensity were used for hydroponics for 3 days, and the water was changed once a day during the hydroponics period.

[0019] (2) Induction culture: Cut off the large leaves of the explants after the treatment in step (1) and cut them into 3-4cm long stems, first soak them in washing powder solution with 2 drops of Dettol for 10 minutes, and then run water Rinse for 1 hour, disinfect with 75% ethanol for 30 seconds in an ultra-clean workbench, wash with sterile water for 3 times, then disinfect with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water for 4 times, absorb water with sterile filter paper, and inoculate into Cluster ...

Embodiment 2

[0024] (1) Collection and treatment of explants: The 1-year-old stems with axillary buds collected from the field were washed with tap water to clean the dust on the surface, and then sprayed with 2% Dettol and placed in an artificial climate box at a temperature of 26°C. 70% humidity, 13 hours of light per day, and 2000lx light intensity were used for hydroponics for 5 days, and the water was changed once a day during the hydroponics period.

[0025] (2) Induction culture: Cut off the large leaves of the explants after the treatment in step (1) and cut them into 3-4 cm long stems, soak them in washing powder solution with 2 drops of Dettol for 15 minutes, and then run water Rinse for 3 hours, disinfect with 75% ethanol for 45 seconds in an ultra-clean workbench, wash 5 times with sterile water, then disinfect with 0.1% mercuric chloride solution for 15 minutes, rinse with sterile water for 5 times, absorb the water with sterile filter paper, and inoculate into Cluster bud ind...

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Abstract

The invention discloses an in-vitro rapid propagation method of cercidiphyllum japonicum. The cercidiphyllum japonicum is the valuable, rare and endangered plant of secondary protection of China, and has extremely high scientific research, and medicinal and ornamental values. At present, the cercidiphyllum japonicum is generally propagated by cottage and grafting and by use of seeds, but due to the propagation methods, the reproduction rate is low and the reproduction period is long, and far fail in meeting the requirements of seedlings for landscaping, scientific research and the like; according to the in-vitro rapid propagation method of the cercidiphyllum japonicum, the in-vitro rapid propagation system of the cercidiphyllum japonicum is established by taking the stem with axillary buds of the cercidiphyllum japonicum as explant and by virtue of processes of explant processing, induced culture, propagation, rooting, acclimatization and transplanting and the like; the propagation coefficient of the cercidiphyllum japonicum can be increased, and then the industrialized seedling production of the cercidiphyllum japonicum can be realized.

Description

technical field [0001] The invention relates to a method for culturing plant tissue in agricultural biotechnology, in particular to a method for in vitro and rapid propagation of japonica. Background technique [0002] Lianxiang tree ( Cercidiphyllum japonicum ) is also known as Wujun tree, ginkgo, Bauhinia leaf wood, etc., [0003] It is a single relict plant of the Tertiary Period, distributed intermittently in China and Japan, and is a rare and endangered plant under the second-class protection in my country. Because of the fragrance of the Lianxiang tree, it can be smelled within a hundred meters. If it is a big tree, the more it shakes, the more fragrant it becomes, so it is called "Lianxiang tree". Lianxiang tree has a wide range of uses. The trunk can be used to make paper. The roots, stems, leaves and fruits all have high medicinal value. Its bark can be taken in water to treat colds and dysentery. The leaves can be used to extract spices. raw material. Lianxian...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G17/00
Inventor 刘祖英
Owner 刘祖英
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