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Method for promoting rapid propagation of cluster buds of butterfly orchid by utilizing LED (Light Emitting Diode) light source

A technology of LED light source and Phalaenopsis, applied in the field of plant tissue culture, to achieve the effect of increasing fresh weight and plant height, reducing production cost and saving energy

Active Publication Date: 2015-02-18
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since different plants have different requirements for light quality and light intensity at different stages of growth and development, the effect of LED on the rapid propagation of Phalaenopsis tissue culture has not been reported yet.

Method used

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  • Method for promoting rapid propagation of cluster buds of butterfly orchid by utilizing LED (Light Emitting Diode) light source
  • Method for promoting rapid propagation of cluster buds of butterfly orchid by utilizing LED (Light Emitting Diode) light source
  • Method for promoting rapid propagation of cluster buds of butterfly orchid by utilizing LED (Light Emitting Diode) light source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) In the experiment, the flower stalks of Phalaenopsis 'Green Bear' (Green Bear) purchased from the Jinying Flower Seedling Promotion Center of the Institute of Environmental Horticulture, Guangdong Academy of Agricultural Sciences were selected as explants. Differentiation was induced on the pedicel axillary bud induction medium, and the induction culture conditions were: culture temperature 25±2°C, relative humidity 50%-60%, light time 12h / d, and cultured in the ordinary tissue culture light environment of the covering newspaper in the previous week That is, when the light intensity is 5±1μmol·m -2 ·s -1 Cultivate for 7 days in the normal tissue culture light environment, after uncovering the newspaper, continue to culture in the normal tissue culture light environment for 45-60 days, that is, when the light intensity is 20±2μmol·m -2 ·s -1 Cultivate for 45-60 days under the ordinary tissue culture light environment to obtain clustered buds;

[0040] (2) Using a ...

Embodiment 2

[0056] What this embodiment is different from Example 1 is that the experimental material that adopts is ' big pepper ' (Big Chilli), and all the other are identical with Example 1.

[0057] The effects of LEDs with different light qualities on the proliferation of 'Big Pepper' are as follows: The proliferation coefficient of 'Big Pepper' was greater under 630nm pure red light and Warm W, which were 59.42% and 59.42% higher than that of the control group, respectively. 46.37%. Judging from the number of clustered buds not less than 3 and the number of explants, the RED (630), RED (660), and Warm W treatments all reached more than 25% (Table 4).

[0058] Table 4 Effects of LEDs with different light qualities on single bud proliferation coefficient of Phalaenopsis 'Big Pepper'

[0059]

[0060] *The results in the table are recorded as mean ± standard error, and different letters after the numbers indicate significant differences, P<0.05.

[0061] The fresh weight of the ro...

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Abstract

The invention belongs to the field of plant tissue culturing, and in particular relates to a method for promoting rapid propagation of cluster buds of a butterfly orchid by utilizing an LED (Light Emitting Diode) light source. The method comprises the following steps: (1) inducing the generation of cluster buds by taking a pedicel of the butterfly orchid as an explant; (2) cutting the cluster buds obtained in the step (1) into simple buds or bunch buds and inoculating the simple buds or the bunch buds into an enrichment medium; and (3) by taking an LED capable of emitting red light or emitting warm white light as a light source, culturing the cluster buds which are obtained in the step (2) and are inoculated into the enrichment medium under the LED capable of emitting red light or emitting warm white light. According to the method, the number of the single buds of the cluster buds is obviously increased; the dry weight, the fresh weight and the plant height of the cluster buds are all increased; and the production cost is lowered and energy conservation is realized.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for promoting the rapid propagation of phalaenopsis buds by using LED light sources. Background technique [0002] Phalaenopsis (Phalaenopsis ssp.) is a plant of the genus Phalaenopsis in the family Orchidaceae. It has a unique flower shape, elegant posture, and long flowering period. It is known as the "Queen of Orchids" and occupies an important position in the flower market at home and abroad. . [0003] The widely used Phalaenopsis propagation method in production is tissue culture technology. The commonly used approach is the cluster bud approach, including several stages of explant-induced cluster buds, cluster bud proliferation, and rooting and strong seedlings, wherein the cluster bud proliferation is the basis for producing a large number of seedlings. The traditional tissue culture light source is a white fluorescent lamp, which generates a lot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱根发任桂萍刘海林
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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