One-step seedling method for rapid propagation of pseudobulbe of phaius tankervilleae

A technology of pseudobulb and Hedinglan, which is applied in the field of one-step seedling rapid propagation of Hedinglan pseudobulb, can solve the problems of long cultivation period, low multiplication coefficient, troublesome operation, etc., and achieve low production cost, high multiplication coefficient, The effect of easy operation

Inactive Publication Date: 2016-07-20
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissue culture technology generally includes primary induction, subgeneration proliferation, rooting and strong seedling cultivation, and seedling hardening and transplanting. Generally, explants are induced and cultured to remove root tissue to achieve proliferation, and then rooted and strengthened seedlings are carried out through rooting culture. The culture cycle Long, cumbersome to operate, low multiplication coefficient, and multiplication coefficient and culture cycle directly determine the production cost of tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A one-step seedling-forming method for the rapid propagation of the pseudobulb of Hedinglan, in which the protocorm of Hedinglan with a root-bud structure is transferred to a proliferation medium for subculture, and after 60-70 days of subculture, a root-bud is obtained. And the proliferating seedlings of clustered pseudobulbs, the proliferating seedlings continue to be subcultured for 20-30 days to obtain test-tube plantlets with a bud height of 3-6 cm and a root length of 1-2 cm for hardening and transplanting, and the formula of the proliferating medium includes: MS, 6-BA1.0mg / L, KT0.5mg / L, TDZ0.2mg / L, NAA0.5mg / L, activated carbon 0.1%, coconut milk 10%, pH 6.0, of which 0.1%, 10% are activated carbon and coconut water account for the mass ratio percentage of the proliferation medium.

[0022] Wherein, the subculture conditions are as follows: the temperature is 24-28° C., the light intensity is 2000 Lx, and the light time is 10 h / day.

[0023] Wherein, the transpla...

Embodiment 2

[0026] A one-step seedling-forming method for the rapid propagation of the pseudobulb of Hedinglan, in which the protocorm of Hedinglan with a root-bud structure is transferred to a proliferation medium for subculture, and after 60-70 days of subculture, a root-bud is obtained. And the proliferating seedlings of clustered pseudobulbs, the proliferating seedlings continue to be subcultured for 20-30 days to obtain test-tube plantlets with a bud height of 3-6 cm and a root length of 1-2 cm for hardening and transplanting, and the formula of the proliferating medium includes: MS, 6-BA2.0mg / L, KT0.5mg / L, TDZ0.5mg / L, NAA0.75mg / L, activated carbon 0.15%, coconut milk 15%, pH 6.0, of which 0.15% and 15% are activated carbon and coconut water account for the mass ratio percentage of the proliferation medium.

[0027] Wherein, the subculture conditions are as follows: the temperature is 24-28° C., the light intensity is 2000 Lx, and the light time is 10 h / day.

[0028]Wherein, the tra...

Embodiment 3

[0031] A kind of one-step seedling-forming method of the rapid propagation of the pseudobulb of Hedinglan, on the basis of embodiment 1, the formula of described proliferation medium is: MS, 6-BA2.0mg / L, KT1.0mg / L, TDZ0. 6mg / L, NAA1.5mg / L, activated carbon 0.2%, coconut milk 20%, pH is 6.0, wherein 0.2%, 20% are respectively the mass ratio percentages of activated carbon and coconut milk in the proliferation medium.

[0032] Except the formula of proliferation medium, others are identical with embodiment 1.

[0033] The method of this embodiment can realize the multiplication of pseudobulbs, and the multiplication factor is 5.68 times. At the same time, it can also realize one-step seedling formation, and the transplanting survival rate reaches 88.2%.

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Abstract

The invention discloses a one-step seedling method for rapid propagation of pseudobulbe of phaius tankervilleae. The one-step seedling method includes transplanting original corm of phaius tankervilleae differentiating the root and bud structure to a multiplication culture medium for subculture, culturing for 60-70 days to obtain multiplication seedlings with roots and buds and clustered peudobulbe, and keeping subculturing the multiplication seedlings for 20-30 days to obtain test-tube plantlets with buds 3-6 cm high and roots 1-2 cm long for acclimatizing and transplanting. The formula of the multiplication culture medium comprises 1.0-2.0 mg / L of MS, 6-BA, 0.5-1.0 mg / L of KT, 0.2-0.6 mg / L of TDZ, 0.5-1.5 mg / L of NAA, 0.1-0.2% of activated carbon, and 10-20% of coconut juice. PH is 6.0. The one-step seedling method can realize multiplication and rooting of phaius tankervilleae by subculture multiplication, and can culture at one step by omitting rooting and culturing. In addition, the one-step seedling method is short in culture period, high in multiplication coefficient and low in production cost.

Description

technical field [0001] The invention relates to the technical field of Hedinglan tissue culture. More specifically, the present invention relates to a one-step seedling-forming method for rapid propagation of the pseudobulb of Hedinglan. Background technique [0002] Phaiustankervilleae (Phaiustankervilleae) belongs to the Orchidaceae Phaiustankervilleae, is a perennial herbaceous plant integrating ornamental and medicinal uses. The flower stem is upright, born on the side of the pseudobulb, 70-120cm long, raceme, with 10-20 flowers, flower diameter about 10cm, the shape of the tube-shaped lip in the corolla is very unique, and the color is different from other petals, The flowers are rich in color, bright and fragrant, and the flowering period lasts for more than 1 month, which has high ornamental value. Heding orchid is also suitable as a parent to breed new orchid varieties by hybridizing with other genera of Orchidaceae. The market value of the successfully hybridized ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 姚绍嫦蓝祖栽凌征柱
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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