Breeding method of tissue-culture seedlings of Honghua kiwi fruits
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A kiwifruit and tissue culture technology is applied in the field of breeding of Honghua kiwifruit tissue culture seedlings, which can solve the problems of inability to realize the industrialized production of Honghua kiwifruit, low axillary bud induction rate, low value-added coefficient, etc. The effect of high axillary bud induction rate and normal leaf shape and color
Inactive Publication Date: 2018-01-09
CHONGQING TIANPEI AGRI TECH +1
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The inventors found in previous work that there are mainly the following problems in the process of kiwifruit tissue culture: (1) the
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[0031] The implementation of the present invention will be described in detail below with examples, so as to fully understand and implement the implementation process of how the present invention uses technical means to solve technical problems and achieve technical effects.
[0032] The invention discloses a method for breeding Honghua kiwifruit tissue culture seedlings, which comprises the following steps:
[0033] 1) Induction of axillary buds from explants: using the stem nodes extracted in the same year as explants, after the explants were sterilized, they were inoculated on the induction medium for axillary bud induction and culture to obtain axillary buds; the induction medium was cultured with improved MS The base was the basic medium, and 6-benzylaminopurine (6-BA) 1mg / L, indolebutyric acid (IBA) 0.1mg / L, carrageenan 6.0g / L and sucrose 30g / L were added to adjust the pH to 5.6 ~5.8, among which, the improved MS medium is based on the MS medium, ammonium nitrate is halv...
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Abstract
The invention discloses a breeding method of tissue-culture seedlings of Honghua kiwi fruits. The breeding method comprises the following steps: adopting stem sections drawn in the current year as explants, after explants are disinfected, inoculating the explants on an induction culture medium to carry out axillary bud induction culture to obtain axillary buds; cutting the obtained primary axillary buds into single buds, transferring into a secondary culture medium for secondary culture to obtain secondary cluster buds; when the secondary cluster buds grow to be 1.5-2cm in height, cutting thecluster buds into single buds, transferring into a rooting culture medium to carry out rooting culture, thus obtaining test tube seedlings; and transplanting the obtained test tube seedlings into a mixed medium with the volume ratio of perlite to peat soil being 1:9 to obtain the tissue-culture seedlings of the Honghua kiwi fruits. The breeding method disclosed by the invention has the advantagesof high axillary bud induction rate, high adventitious bud proliferation coefficient, high rooting rate, high survival rate of transplantation of test tube seedlings, strong growth of bud seedlings, normal shapes and colors of leaves, subcutaneous rooting and fast germination of new leaves and the like, and can meet the need of large-area and large-scale cultivation of the Honghua kiwi fruits.
Description
technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a breeding method for Honghua kiwifruit tissue culture seedlings. Background technique [0002] Kiwi fruit (Actinidia chinensis Planch), also known as fox peach, vine pear, sheep peach, muzi, mao fruit, kiwi fruit, hemp vine fruit, etc., the fruit shape is generally oval, the appearance is greenish brown, and the skin is covered with thick hairs. Edible, it contains bright green flesh and a row of black seeds. Because macaques like to eat, it is named kiwi fruit; it is also said that it is named because the skin is covered with hair and looks like a macaque. It is a fresh, tender, nutritious and delicious fruit. In addition to organic substances such as kiwifruit, proteolytic enzymes, tannin pectin and sugars, as well as trace elements such as calcium, potassium, selenium, zinc, germanium, and 17 kinds of amino acids needed by the human body, kiwifruit also contai...
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