111 results about "Macaque" patented technology

The present invention relates to the identification of macaque antibodies to human B7.1 and B7.2 by screening of phage display libraries or monkey heterohybridomas obtained using B lymphocytes from B7.1 and / or B7.2 immunized monkeys. More specifically, the invention provides four monkey monoclonal antibodies 7B6, 16C10, 7C10 and 20C9 which inhibit the B7:CD28 pathway and thereby function as effective immunosuppressants. The invention further provides the complete DNA and amino acid sequences of the light and heavy chain of three primatized antibodies derived from those monkey monoclonal antibodies which bind B7.1 and possibly B7.2, primatized 7C10, primatized 7B6 and primatized 16C10. These primatized and monkey antibodies may be used as specific immunosuppressants, e.g., for the treatment of autoimmune diseases and to prevent organ transplant rejection.

The invention relates to the technical field of an animal feed, and in particular relates to an experimental macaque feed and a feeding method thereof. The experimental macaque feed is characterized by being formed by uniformly mixing main materials, auxiliary materials, microelements containing vitamin E, table salt and white sugar, wherein the main materials comprise flour and / or starch type plant fruit tuber root powder; and the auxiliary materials comprise fish meal and / or bone meal. The experimental macaque feed has the effects of promoting the growth of experimental macaques, improving the breeding quality, improving the physical property and the immunological competence of the macaques, preventing the occurrence of macaque scurvy and eliminating a macaque unhairing problem; and the standard refined preparation is adopted, feed requirements of experimental animal feeding industry are improved, and the standardization of experimental animals is realized.

Disclosed herein are sequences, molecules and methods used to suppress the expression of HD genes encoding for huntingtin protein in primates including Macaca mulatta and Homo sapiens. These sequences, molecules and methods aid in the study of the pathogenesis of HD and can also provide a treatment for this disease by reducing HD mRNA without causing death, locomotor impairment or cellular alterations of the Macaca mulatta and Homo sapiens.

The invention relates to flight equipment for tracing and monitoring wild animals. The equipment comprises a control device, a communication device, a positioning device, a shooting device, a sound collection device, a route determining device, an electricity quantity detection device and a flight determining device. The positioning device is respectively connected with the communication device and the control device. The positioning device is used for positioning the flight equipment for tracing and monitoring wild animals and then transmitting the obtained positioning signal to the communication device and the control device. The communication device is used for sending the positioning signal so as to be in communication with the outside. The shooting device is used for video / image shooting. The sound collection device is used for collecting environmental sounds. The electricity quantity detection device is used for detecting the electricity quantity of the power supply of the flight equipment for tracing and monitoring wild animals. The control device controls the overall operation of the flight equipment for tracing and monitoring wild animals so as to control the communication device, the positioning device, the shooting device, the sound collection device, the route determining device, the electricity quantity detection device and the flight determining device. According to the technical scheme of the invention, wild animals can be traced and tracked in real time, and the activity images of the wild animals are recorded. The equipment is timely in positioning and tracing effect and accurate in monitoring effect.

The invention relates to the technical field of animal breeding, in particular to a breeding method for an experimental macaque. Main feed containing vitamin E is fed to the macaque in at least one of a suckling period, a growth period and an adult period; cefotaxime or ceftriaxone sodium or ceftazidime is added into the feed continuously for 5-7 days every 1-2 months in at least one of the growth period and the adult period; a young macaque is independently fed in the suckling period, feeding quality is further improved, the health condition of the macaque can be timely discovered and managed, the survival rate of the young macaque is improved, and the young macaque is normal in growth and development, flexible in limbs and physically strong. Moreover, the probability and the risk of bacterial dysentery infection of the macaque are reduced, and common health of the macaque and workers is ensured. A normalized experimental animal breeding standard is provided, and the breeding method is easy to popularize and use.

The invention belongs to the technical field of animal immunization biochemical detection, and particularly relates to a progesterone colloidal gold immunochromatography test strip for monkey early pregnancy detection. The test strip comprises a PCV bottom plate, an absorption pad, a nitrocellulose membrane, water absorption filter paper, a gold mark pad and a sample pad, wherein the nitrocellulose membrane is provided with a detection line and a quality control line, and the absorption pad, the nitrocellulose membrane, the gold mark pad and the sample pad are sequentially pasted to the PCV bottom plate. The test strip is characterized in that the gold mark pad contains progesterone monoclonal antibodies; the gold mark pad containing the progesterone monoclonal antibodies and the water absorption filter paper are arranged at the two ends of the nitrocellulose membrane respectively in an overlapping mode, and meanwhile the gold mark pad containing the progesterone monoclonal antibodies and the sample pad are arranged in an overlapping mode; the monoclonal antibodies capable of detecting monkey early pregnancy are excreted by a hybridoma cell strain with the preservation number of CCTCC NO:C2015170. The test strip is high in sensitivity and easy and convenient to operate and has great significance for improving breeding monitoring of monkeys such as macaques and snub-nosed monkeys.

A focal cerebroischemia model for researching and treating human cerebral thrombus is created through providing a macaca mulatta, and introdicing a microspherical catheter to block the start position of cerebral artery.

The invention provides a method for collecting semen of primates of macaque by electro stimulation, belonging to the method of animal reproduction, in particular to the method for collecting sperm of primates of macaque. In the invention, aluminium foil electrodes coated by absorbent cotton infiltrated by saline encircles around the penis of the anesthetized animal and is connected with the impulse power; the voltage begins from 0V, and stimulation is conducted for 10-15s continuously when each 10V is increased, until the voltage reaches 60V; the process is circulated for 6 to 7 times when necessary, with an interval of 20-30s between two stimulations; and the collected semen can be used for fertilization after incubation and separation. The invention can improve the success rate for collecting semen of animals, and has no pollution; the semen collected in the invention has similar index as that of the semen gained through natural mating and can be applied to external fertilization and artificial fertilization.

The invention discloses a construction method of a depression model for crab-eating macaques at adolescence and juvenility. The construction method comprises the steps of selecting 10 male crab-eatingmacaques at the adolescence, and dividing the selected crab-eating macaques into a chronic unpredictability stimulus group and a control group; performing 7-day daily treatment, and after adaptationis completed, performing 8-day baseline test; performing 5-cycle experiment while performing daily treatment on the chronic unpredictability stimulus group, including 7-day chronic unpredictable lightstimulus and 4-day behavior observation, performing daily treatment on the control group in the whole experiment process, and after every cycle is completed, also performing 4-day behavior observation; after the whole experiment cycle is completed, also performing apple desiring experiment and human intruder experiment on the chronic unpredictability stimulus group and the control group to respectively test the depression action and the anxiety-like action of the crab-eating macaques; collecting hair and plasma and performing cortisol content test; and finally, performing contrast with adolescent depression patients by a plasma metabonomics method. The model can provide reference for researching a potential mechanism of depression of teenagers in the future.

The invention relates to an ultralow temperature cryopreservation method of macaca primate semen, belonging to an animal breeding method and comprising the following steps: dissolving Tris, TES, lactose, glucose, raffinose, sodiumpenicillinG, streptomycin sulphate and the like in water, centrifuging, taking supernate, preparing the supernate into semen diluent, adding glycerol to prepare deicing fluid, diluting the semen by using diluent, cooling the temperature to 4 DEG C, mixing with the dicing fluid precooled to 4 DEG C with the same volume, subpackaging into frozen straws, cooling by utilizing liquid nitrogen steam, and placing in liquid nitrogen at the temperature of minus 196 DEG C for preservation. When in use, the semen is defrosted and revived. The method in the invention can improve the revived motion range, the survival time, the plasma membrane and acrosome completeness and the fertility rate of the frozen sperm of the macaca primate.

The invention relates to a method for separating and culturing macaque adult hepatic precursor cells, in particular to a separation and culture solution of the macaque adult hepatic precursor cells, belonging to a separation and culture solution of animal precursor cells. The method comprises the following steps: digesting a small quantity of hepatic tissues obtained by a macaque liver removal surgery or other ways by utilizing IV-type collagenase; collecting and suspending the cells in 10% of a fetal calf serum DMEM for culture; selecting and then inoculating a single cell block in the culture plate of a rat tail collagen bed board, wherein the culture solution is the special one; and finally sorting E-cad+cells from the cultured cells by the flow cytometry to obtain the target cells. The cells separated by the method of the invention can be applied to cell differentiation mechanism study and can become ideal master cells for treating late-period hepatic diseases.

The invention relates to a feed additive for a macaque and the application thereof. The feed additive is composed of 3-6 parts of pyrola, 3-6 parts of epimedium herb, 3-6 parts of D-fructose and 2-5 parts of L-arginine by weight. A preparation method includes grinding dried pyrola and epimedium herb to prepare 60-mesh powder, weighing the four components according to weight percentage, evenly mixing the components and storing the components at the shady and dry position in sealing mode. The feed additive is added into a full-price feed for macaque propagation and is continuously used for 45 to 60 days after a baby macaque is weaned in propagation process. The feed additive has the advantages of improving macaque propagation.

The invention discloses a PCR method for detecting the mutation of TRIM4 loca of animals of macaca, which comprises the following steps of: (1) extracting DNA from the hair of the animals of the macaca; (2) performing PCR amplification by taking SEQ ID NO:1 and SEQ ID NO:2 as primers; and (3) determining the TRIM5 loca of the animals to be detected are not mutated if an amplification product is one 2,300 to 2,400 bp segment, determining the TRIM5 loca are subjected to heterozygous mutation if the amplification products are the 2,300 to 2,400 bp segment and a 3,000 to 3,150 bp segment respectively, and determining the TRIM5 loca are subjected to homozygous mutation if the amplification product is only the 3,000 to 3,150 bp segment. The method of the invention has the advantage of accurately detecting the TRIMCyp genotypes of the animals of the macaca, and can be used for surveying the distribution of TRIMCyp in macaques in China and screening positive individuals.

The invention discloses a training method for macaque to correctly contact a screen and a screen contact training device. An auxiliary module is used for standardizing macaque behavior motions, helping the macaque to correct bad habits, and improving the test efficiency and accuracy. At the same time, the training process of the macaque is simplified, and the time spent by the macaque in task learning is greatly shortened. The training method and the training device help the macaque to perform touch screen tests efficiently and to complete more difficult behavioral paradigms so as to improve and perfect existing macaque behavior testing works, and provide some help for neurobiology research in the future.

The invention discloses a positive-pressure directional flow ten thousand-level purification monkey feeding laboratory room. The positive-pressure directional flow ten thousand-level purification monkey feeding laboratory room is characterized in that feeding separating space is formed in the laboratory room, curtains are arranged at two sides of the feeding separating space, a directional flow air inlet barrier is arranged at the front part of the feeding separating space, the directional flow air inlet barrier is provided with an upper guiding rail and a lower guiding rail, and the upper guiding rail and the lower guiding rail are provided with folding door leaves; each folding door leaf is provided with a fixed hole plate and a sliding hole plate in an assembling way, and micro ventilation holes are evenly formed in the fixed hole plates and the sliding hole plates; two sides of the sliding hole plates are provided with adjusting jackscrews and return springs in the assembling way; the front upper parts of the folding door leaves are provided with air blowers; an air outlet is formed in the rear part of the feeding separating area. According to the positive-pressure directional flow ten thousand-level purification monkey feeding laboratory room disclosed by the invention, the indexes of the air cleanliness, the air falling bacterium and the like of a barrier environment are enabled to reach a ten thousand-level purification level, the animal experiment quality is effectively ensured, and the laboratory room is prevented from being polluted; the using needs on different functions of rhesus monkey experiments are met through the adjustment on directional flow air flow inlet flux; by enabling small directional flow barrier spaces to be separated, multi-purpose rhesus monkey experiments can be conveniently carried out, and interaction and cross infection can be avoided.

The invention relates to a method for establishing a model of aflatoxin inducing macaque liver fibrosis. The method comprises the following steps: with macaque as a model animal, during the model making, adding 0.5mg / kg aflatoxin to the drinking water of macaque for sucking, twice every month at an interval of 2 weeks for 8 successive months; feeding the macaque with macaque commercial maintenance-period feed containing more than 19% of protein and more than 4% of crude fat; performing pathological analysis every 2 months; and 8 months later, performing pathological detection to show obvious liver fibrosis symptoms. The method provided by the invention is safe and reliable, shortens the model making time of an animal model, and is of great significance to screening and predicting effective drugs for controlling liver fibrosis and even liver cancer as an experimental foundation.

This invention discloses one feeding method decreasing death rate of introduced macaque, which integrates many factors, makes improvement in feedstuff, quarantine, cage tools and floor, etc. The method has low investment, but clearly effect, and is convenient and substantial. This method can control the death rate of introduced macaque with three months quarantine under 1% and the total death rate of animals not more than 3%, compared to industry regulate of 7.5% with three months, which decreases more than 4.5% and not only saves national resources but also enhances corporation's economic benefit.

The invention discloses a macaque emotion recognition method and system based on time-frequency double-branch features, and the method comprises the steps: inputting collected macaque sounds into a pre-established and trained macaque emotion recognition model, and obtaining corresponding accurate emotion types; wherein the accurate emotion types comprise friendly emotion, attack or threat emotion, falling or compliance emotion and non-emotion; and the macaque emotion recognition model is used for extracting intermediate features of macaque sounds, performing separation and extraction on time domain features and frequency domain features based on time-frequency double branches, and determining corresponding accurate emotion categories through fusion and classification. According to the method and the system, original sound data is used as network input, emotion-related intermediate features are extracted from sound, voice preprocessing is not needed, the algorithm process is simplified, then time domain and frequency domain features are separated and extracted, compression features of the sound are obtained from different angles, the time domain and frequency domain features are fused, the expressive power of the features is enhanced, and the identification accuracy is effectively improved.

The invention discloses real-time fluorescence quantitative PCR detection primers and probe for SRV (simian type D retrovirus), a detection kit and a detection method. The detection primers and the probe are designed on the basis of SRV-1, SRV-2, SRV-3 and SRV / D-T gene conserved sequences, and the real-time fluorescence quantitative PCR detection method for the SRV is established. Compared with other detection methods, the detection method has higher sensitivity and specificity, can detect a large quantity of samples simultaneously, is low in cost and is suitable for large-scale SRV detection for a monkey field. Reliable background information can be provided for scientific research of selection of macaca fascicularis and macaques on the basis of high SRV detection rate, and monkeys carrying the SRV can be discovered and isolated in advance.

The present invention provides a monoclonal antibody against a filamentous virus GP protein and an application of the monoclonal antibody. A heavy chain variable region of an antigen-binding fragmentof the monoclonal antibody comprises an amino acid sequence shown as SEQ NO:7; and a light chain variable region of the antigen-binding fragment comprises an amino acid sequence shown as SEQ NO:8. Theantigen-binding fragment is obtained from an adult Chinese macaque monkey body, after humanization, the antigen-binding fragment has binding activity to Zaire-type GP protein, Sudan-type GP protein and Marburg virus GP protein of filamentous virus family. The obtained antibody has high homology with human genes, realizes humanization, is good in stability, has a relatively strong affinity for theGP proteins, can be potentially applied to antigen detection of filamentous viruses including Ebola viruses and Marburg viruses, detection and identification of clinical samples of filamentous virusinfection, development of filamentous virus inhibitors, etc.

The present invention provides improved chimeric glycoproteins (GPs) and improved lentiviral vectors pseudotyped with those glycoproteins. Also provided are methods and compositions for making such glycoproteins and vectors, and improved methods of in vitro and in vivo transduction of cells with such vectors. Improved chimeric GPs encode the extracellular and transmembrane domains of GALV or RD114 GPs fused to the cytoplasmic tail of MLV-A GP. Vectors pseudotyped with these GAL V / TR and RD 114 / TR GP chimeras have significantly higher titers than vectors coated with the parental GPs. Additionally, RD114 / TR-pseudotyped vectors are efficiently concentrated and are resistant to inactivation induced by the complement of both human and macaque sera. RD114 GP-pseudotyped lentiviral vectors have particular utility for in vivo gene transfer applications.

The invention discloses a quality control method of monkey bezoar and cow-bezoar powder. The quality control standards of the former monkey bezoar and cow-bezoar powder is revised, lamella distinguish method of bilirubin, monkey bezoar, grassleaf sweelflag rhizome, alpinia zerumbet and licorice is added, lamella check method of hyodesoxycholic acid is also added, and further assay method of cinnabar and cholic acid is added. The quality control method of the monkey bezoar and cow-bezoar powder of the invention has a strong specificity, and the quality of the product is more effectively controlled, further the safety and the efficiency of medicines taking by human bodies are guaranteed.

The invention relates to a feed additive used in a baby macaque period for a macaque and the application thereof. The feed additive includes the following raw materials by weight: 6-10% of mangnolia officinalis, 4-7% of codonopsis, 3-5% of angelica sinensis, 10-14% of hawthorn, 4-7% of liquorice, 15-20% of fried barley malt, 32-38% of caramel, 3-6% of pine carbon and 4-6% of choline. A preparation method includes grinding the mangnolia officinalis, the codonopsis, the angelica sinensis, the hawthorn, the liquorice, the fried barley malt, the pin carbon and the pine carbon, screening the mixture through a 60-mesh screen to prepare powder, weighing and preparing according to weight percentage, evenly mixing and storing in sealing mode at the shady cool position. The feed additive is added in a full steam cake feed of a baby weaning macaque according to the dosage of 1-3% and is used continuously for 90 days. The feed additive has the advantages of effectively reducing stress reaction of the organism, the stomach and the intestine caused by food change of the baby weaning macaque, simultaneously, harmonizing the spleen and the stomach, improving appetite, promoting digestive absorption of the stomach and the intestine, preventing enteritis and improving the immunity function of the mechanism.

The invention discloses an animal fixing device. The animal fixing device comprises a head fixing module, a trunk placing module and a bearing module, wherein the head fixing module, the trunk placingmodule and the bearing module are positioned at the front end, the middle and the tail part of the animal fixing device, the head fixing module is detachably connected with the trunk placing module,and the trunk placing module is detachably connected with the bearing module. The invention further discloses an application of the animal fixing device in nuclear magnetic resonance imaging of largeanimals, and the large animals are preferably monkeys, at least comprising cynomolgus monkeys and macaques. With the technical scheme, the influences of the body movement of the animals on imaging aregreatly improved, thus the data collecting quality is improved, and the experiment efficiency is further improved; the fixing device is compatible with a 9.4T nuclear magnetic resonance imaging device; by adopting the 3D printing technology, the cost of the product is greatly reduced, and meanwhile, the product forming speed is improved.