17 results about "Macaque" patented technology

AAV vectors are ideally suited for long-term delivery of a combination of broadly neutralizing antibodies to achieve sterilizing immunity to HIV. Unfortunately, host immune responses to the delivered antibody have severely limited the efficacy. The TLR9 pathway has been identified in initiating adaptive immune responses against AAV and delivered bNAbs. To enhance this strategy, we validated Hydroxychloroquine, a known drug inhibitor of the TLR9 pathway, as a pretreatment to AAV delivery to avoid anti-drug antibody responses. TLR9 signaling inhibition was validated using a HEK-Blue hTLR9 reporter cell line and CpG stimulation. Hydroxychloroquine was also validated in a 3-macaque trial where AAV9-3BNC117 and AAV9-10-1074 were administered along with 3 doses of hydroxychloroquine once a week starting 1 week before AAV inoculation. Unlike historical controls, where 3BNC117 and 10-1074 expression is lost within the first 4-5 weeks, 2 macaques maintained 10-1074 expression and 1 macaque maintained 3BNC117 for the duration of the trial. Anti-3BNC117 antibodies were only observed in 2 of the 3 macaques and were significantly delayed. Anti-10-1074 antibody responses were a log lower than typically observed in historic controls. The use of hydroxychloroquine as a pretreatment for AAV inoculation is a promising strategy. The significant decrease in anti-10-1074 antibody levels and the successful delivery of 10-1074 in 2 macaques and 3BNC117 in 1 macaque was very encouraging. Extending the dosage of hydroxychloroquine beyond 3 doses may be sufficient to observe long-term bNAb expression in all animals and further decrease ADA responses. Together, these data suggest that the short-term treatment of hydroxychloroquine at the time of AAV inoculation has a meaningful impact on tolerance induction to our AAV-delivered bNAbs.

The invention discloses a macaque cytomegalovirus detection kit supporting multi-mode interpretation and a detection method, and particularly relates to the technical field of virus detection. The kit comprises a nucleic acid quick release reagent, a primer group, an isothermal amplification reaction system, a CRISPR (clustered regularly interspaced short palindromic repeats) detection system, a visual detection component, positive and negative controls, related buffer solutions, enzymes and the like. The detection method integrates nucleic acid extraction-free, ERA isothermal amplification and CRISPRCas12a technologies, realizes rapid amplification and specific detection of target virus nucleic acid, and supports three result interpretation modes of fluorescence, ultraviolet visualization and a lateral flow chromatography test strip. The method has high sensitivity and specificity, the whole process from sample treatment to result output can be completed within 50 min, complex instruments and nucleic acid purification steps are not needed, operation is easy and convenient, cost is low, and the method is suitable for on-site rapid screening of the macaque cytomegalovirus and basic-level facility application.

The invention discloses a biomedicine-based macaque saliva detection analyzer, and relates to the technical field of macaque saliva detection.The biomedicine-based macaque saliva detection analyzer comprises a machine body and a display screen, the display screen is arranged on the surface of the machine body, a receiver is arranged at the front end of the machine body, and the receiver detects collected saliva and displays a detection result on the display screen; the surface of the receiver is connected with an absorption pipe, clamping rods are rotatably installed on the two sides of the bottom of the machine body, an air cylinder is installed at the bottom of the machine body, macaque saliva is collected through the absorption pipe, it is ensured that a safe distance is kept between a collector and a macaque during saliva collection, and a medical sponge is used for smearing a phagostimulant to attract the macaque to actively chew; saliva secreted when the macaque chews is collected and detected in time through the absorption tube, struggling of the macaque in the saliva collection process is avoided, the personal safety of collection personnel is guaranteed, the components of the collected saliva are stable, and the accuracy of a detection result is guaranteed.

The utility model discloses an observation system for monitoring the plant ingestion habit of macaque, which comprises a support rod, the top end of the support rod is fixedly connected with a first monitoring probe, and the outer wall of one side of the support rod is fixedly connected with a second monitoring probe and a signal transceiver. The other side of the supporting rod is fixedly connected with two bearing plates which are symmetrically arranged, rotating grooves are formed in one sides of the two bearing plates, threaded rods are rotationally connected into the rotating grooves, the thread rotating directions of the two sides of the central point of the horizontal axial section of each threaded rod are opposite, and the two ends of each threaded rod are fixedly connected with traction plates; one end of the traction plate is fixedly connected with an arc-shaped clamping plate, a supporting frame is arranged between the two bearing plates, and the supporting frame is fixedly connected with the supporting rod. The monitoring devices at different positions can be used for observing ingestion and daily activities of macaque, and the whole device is convenient to disassemble and assemble through the arrangement of the clamping and fixing assembly; and popularization and use are facilitated.

The present application relates to the technical field of magnetic resonance imaging system, especially to a conscious monkey head and coil fixing device and a magnetic resonance imaging system, the conscious monkey head and coil fixing device, including a monkey box for accommodating the monkey body, one end face of the monkey box is an upward inclined plane, a head hole for exposing the monkey head is formed in the middle part of the inclined plane, and a neck positioning module, left and right positioning modules, an upper end positioning module and a lower end positioning module are respectively arranged on the outer surface of the monkey box, through the above arrangement, the problem that the fixing device in the existing magnetic resonance imaging system is difficult to fix the head of a conscious macaque and the conscious macaque is prone to restlessness is solved.

The invention belongs to the technical field of animal model construction, and particularly relates to a spinal cord hypertension syndrome disease model modeling method and application. A guinea pig or a macaque is used as an experimental animal, and a composite biostimulation material and an accurate pressure regulation and control modeling system are combined to realize modeling. The composite biostimulation material is composed of a core pressure slow-release layer, a middle biocompatible coating and a surface targeting binding layer, has excellent biocompatibility, degradability and targeting property, and can reduce non-specific inflammatory response; the precise pressure regulation and control modeling system comprises a pressure sensing module, a micro-injection module, a real-time monitoring module and a main control module, and can dynamically regulate and control the pressure in the spinal cord in a closed-loop mode. The modeling method is easy and convenient to operate and good in repeatability, the model building success rate exceeds 95%, the pathological features are highly similar to clinical features and can last for 4 weeks, a reliable experimental model is provided for mechanism research, drug screening and therapeutic schedule evaluation of the spinal cord high pressure syndrome, and the modeling method is suitable for large-scale popularization.

Herein is reported an anti-transferrin receptor antibody that specifically binds to a human transferrin receptor and a macaque transferrin receptor, comprising i) a humanized heavy chain variable domain derived from the heavy chain variable domain of SEQ ID NO: 01, and ii) a humanized light chain variable domain derived from the light chain variable domain of SEQ ID NO: 26, wherein the antibody has a dissociation rate for the human transferrin receptor equal to or less than (i.e., at most) the dissociation rate of the anti-transferrin receptor antibody 128.1 for the macaque transferrin receptor wherein the dissociation rate is determined by surface plasmon resonance, and the anti-transferrin receptor antibody 128.1 has a heavy chain variable domain of SEQ ID NO: 64 and a light chain variable domain of SEQ ID NO: 65.

The present invention pertains to the nanoencapsulation of siRNA and other biologics in phospholipid nanosomes for the improved delivery of siRNA and other biologics to targeted diseased human or animal organs and human or animal cells and apparatus and methods for making the same. In embodiments of the present invention, novel siRNAs were designed to down regulate CCR5 and CD4, based on an analysis of all known alternative transcripts for each gene from both human and monkey (Macaca mulatta) genomes. Embodiments of the present invention feature supercritical, critical and near critical fluids. Embodiments of the present invention also pertain to down regulation of CXCR4 receptor and targeting of nanosomes containing specific siRNA sequences to cells expressing those receptors on the cell surface by coating them with specific ligands. These include ligands for the receptors CCR5, CD4 and CXCR4.

The invention provides a macaque individual identification method and system, and the method comprises the steps: obtaining a to-be-detected image containing a to-be-identified macaque individual; the face region detection model is obtained by training a preset YOLOv10 model based on a first feature curve corresponding to a macaque face region in the training macaque individual image; cutting the image data based on the target bounding box, and inputting a facial region image obtained after cutting into a trained facial feature recognition model for feature extraction and recognition processing to obtain a recognition result of the to-be-detected image; and based on the identification result, determining archive information of the corresponding macaque individuals in a macaque individual database. According to the scheme, the macaque individual identification accuracy and efficiency can be improved.

The application discloses a macaque detection method based on a YOLOv7 network and a Deepsort network, inputs a macaque motion video sequence into the network, extracts and processes frame images, obtains a prediction result by extracting features of the input images through the optimized YOLOv7 network, sends the prediction result into the Deepsort network again as input information to obtain a prediction frame sequence, and finally detects and processes missing values of the prediction frame sequence. The application is based on a single-stage target detection algorithm, combines a time sequence transfer module (TSM) to extract space-time information of a target video sequence, improves the precision of macaque detection, concatenates a Deepsort algorithm to the detection output position to re-process and optimize the output result, and solves the problems of difficulty in feature selection, low recognition rate, influence of factors such as brightness, contrast change and shadow in the image, and low detection precision under the influence of cage shielding.

The invention discloses a macaque skin squamous carcinoma cell line MCSCC14397 and application of the macaque skin squamous carcinoma cell line MCSCC14397. The macaque skin squamous carcinoma cell line is named as macaque skin squamous carcinoma cell line MCSCC14397, and the preservation number of the macaque skin squamous carcinoma cell line is CCTCC (China Center For Type Culture Collection) NO: C2025156. The cell line disclosed by the invention has similar protein expression with primary tumor tissues, has chromosome abnormal karyotype, in-vitro tumor formation capability and stable proliferation characteristics, and simultaneously shows remarkable genome instability (such as high-frequency Cgt, T mutation and structural variation) and inflammation signal activation. The MYO10 gene expression in the cell line is up-regulated and drives DNA damage reaction and inflammatory factor expression, and unique advantages are provided for application of the cell line in skin squamous cell carcinoma mechanism research, drug screening and drug efficacy evaluation. The cell line disclosed by the invention can be used as an effective cell model, can be used for establishing a xenotransplantation animal model, and can provide a research basis for deeply researching the occurrence, development, metastasis mechanism and drug resistance mechanism of human skin squamous cell carcinoma as well as innovative drug development and screening.

The invention discloses application of a biomarker HMDB0254633 in detection of HBB gene mutation of macaque, relates to the technical field of biology, and solves the technical problem of lack of a specific biomarker for early detection of thalassemia intestinal complications caused by HBB mutation. The key points of the technical scheme are as follows: the invention provides application of the biomarker HMDB0254633 in preparation of a quantitative detection product for macaque HBB gene mutation, the chemical name of the biomarker HMDB0254633 is 3-oxooctadecanoic acid, the molecular formula of the biomarker HMDB0254633 is C18H34O3, the quantitative detection product is a reagent or a kit, and the invention further provides a method for quantitatively detecting HBB gene mutation. Comprising the following steps: 1) extracting metabolites in excrement; 2) detecting metabolites by using a quantitative detection product; the effects of providing a high-specificity non-invasive marker and a detection tool and realizing early-stage rapid and non-invasive detection are achieved.

The application discloses a kind of based on the relevance calculation method of human face and macaque face with acne, belong to digital image processing field and depth learning field.The application designs image processing, neural network, multi-modal fusion, contour similarity calculation, data set production and acquisition.The application eliminates the individual uniqueness by enlarging data set, superimposed fusion to the face segmentation result of many people, contour fitting, explores commonality problem.The conclusion whether there is relevance between human face acne occurrence area and macaque face hair area is obtained by the similarity calculation of fitting contour.

The application discloses a sgRNA for targeting rhesus monkey ATM Gene knockout, and belongs to the technical field of biology. The sgRNA for targeting the rhesus monkey ATM gene is shown in any one of SEQ ID NO: 2, 6, 7 and 8. The application further provides a method for targeting and knocking out the rhesus monkey ATM gene by using the sgRNA: multiple sgRNAs are designed based on a CRISPR / Cas9 system and are targeted to the rhesus monkey ATM gene. ATM The sgRNA for knocking out the rhesus monkey ATM gene is mixed with a Cas9 mRNA mixture, and the mixture is jointly injected into a rhesus monkey embryo, and then the embryo is transplanted into a female rhesus monkey, so as to obtain ATM a rhesus monkey with the gene knocked out. The method provides a new method for establishing a non-human primate AT animal model, and provides a research basis for studying a cerebellum degenerative phenotype, pathological characteristics and inherent molecular mechanism of an AT patient, analyzing a pathogenic mechanism and drug screening.

Herein is reported an anti-transferrin receptor antibody that specifically binds to a human transferrin receptor and a macaque transferrin receptor, comprising i) a humanized heavy chain variable domain derived from the heavy chain variable domain of SEQ ID NO: 01, and ii) a humanized light chain variable domain derived from the light chain variable domain of SEQ ID NO: 26, wherein the antibody has a dissociation rate for the human transferrin receptor equal to or less than (i.e., at most) the dissociation rate of the anti-transferrin receptor antibody 128.1 for the macaque transferrin receptor wherein the dissociation rate is determined by surface plasmon resonance, and the anti-transferrin receptor antibody 128.1 has a heavy chain variable domain of SEQ ID NO: 64 and a light chain variable domain of SEQ ID NO: 65.

The utility model discloses a special fixing device suitable for MRI (Magnetic Resonance Imaging) of a macaque spine, which comprises a macaque box which is shaped like a cuboid, the head end of the macaque box is narrower, and a macaque can vertically lie in the macaque box in an anesthetic state; the hericium erinaceus fixing device and the monkey body fixing device are installed on the left side and the right side of the head end and the body end of the monkey box through bolts respectively and are telescopic, each fixing device is composed of two parts, the bolts and a baffle are rotated, the outer layer of the baffle is wrapped by a sponge cover, after the body position of the macaque is adjusted, the fixing devices are rotated, the head and the hip of the macaque are fixed, and then the spine of the macaque is subjected to magnetic resonance imaging. The body position of a macaque in an anesthesia state can be corrected through the macaque box, the head and the hip of the macaque are stably fixed, scoliosis of the macaque is prevented, the macaque box also plays a role in isolating from an examination bed, and equipment is prevented from being polluted by macaque excrement.