7 results about "Macaque" patented technology

AAV vectors are ideally suited for long-term delivery of a combination of broadly neutralizing antibodies to achieve sterilizing immunity to HIV. Unfortunately, host immune responses to the delivered antibody have severely limited the efficacy. The TLR9 pathway has been identified in initiating adaptive immune responses against AAV and delivered bNAbs. To enhance this strategy, we validated Hydroxychloroquine, a known drug inhibitor of the TLR9 pathway, as a pretreatment to AAV delivery to avoid anti-drug antibody responses. TLR9 signaling inhibition was validated using a HEK-Blue hTLR9 reporter cell line and CpG stimulation. Hydroxychloroquine was also validated in a 3-macaque trial where AAV9-3BNC117 and AAV9-10-1074 were administered along with 3 doses of hydroxychloroquine once a week starting 1 week before AAV inoculation. Unlike historical controls, where 3BNC117 and 10-1074 expression is lost within the first 4-5 weeks, 2 macaques maintained 10-1074 expression and 1 macaque maintained 3BNC117 for the duration of the trial. Anti-3BNC117 antibodies were only observed in 2 of the 3 macaques and were significantly delayed. Anti-10-1074 antibody responses were a log lower than typically observed in historic controls. The use of hydroxychloroquine as a pretreatment for AAV inoculation is a promising strategy. The significant decrease in anti-10-1074 antibody levels and the successful delivery of 10-1074 in 2 macaques and 3BNC117 in 1 macaque was very encouraging. Extending the dosage of hydroxychloroquine beyond 3 doses may be sufficient to observe long-term bNAb expression in all animals and further decrease ADA responses. Together, these data suggest that the short-term treatment of hydroxychloroquine at the time of AAV inoculation has a meaningful impact on tolerance induction to our AAV-delivered bNAbs.

The invention discloses a macaque cytomegalovirus detection kit supporting multi-mode interpretation and a detection method, and particularly relates to the technical field of virus detection. The kit comprises a nucleic acid quick release reagent, a primer group, an isothermal amplification reaction system, a CRISPR (clustered regularly interspaced short palindromic repeats) detection system, a visual detection component, positive and negative controls, related buffer solutions, enzymes and the like. The detection method integrates nucleic acid extraction-free, ERA isothermal amplification and CRISPRCas12a technologies, realizes rapid amplification and specific detection of target virus nucleic acid, and supports three result interpretation modes of fluorescence, ultraviolet visualization and a lateral flow chromatography test strip. The method has high sensitivity and specificity, the whole process from sample treatment to result output can be completed within 50 min, complex instruments and nucleic acid purification steps are not needed, operation is easy and convenient, cost is low, and the method is suitable for on-site rapid screening of the macaque cytomegalovirus and basic-level facility application.

The invention discloses a biomedicine-based macaque saliva detection analyzer, and relates to the technical field of macaque saliva detection.The biomedicine-based macaque saliva detection analyzer comprises a machine body and a display screen, the display screen is arranged on the surface of the machine body, a receiver is arranged at the front end of the machine body, and the receiver detects collected saliva and displays a detection result on the display screen; the surface of the receiver is connected with an absorption pipe, clamping rods are rotatably installed on the two sides of the bottom of the machine body, an air cylinder is installed at the bottom of the machine body, macaque saliva is collected through the absorption pipe, it is ensured that a safe distance is kept between a collector and a macaque during saliva collection, and a medical sponge is used for smearing a phagostimulant to attract the macaque to actively chew; saliva secreted when the macaque chews is collected and detected in time through the absorption tube, struggling of the macaque in the saliva collection process is avoided, the personal safety of collection personnel is guaranteed, the components of the collected saliva are stable, and the accuracy of a detection result is guaranteed.

The present application relates to the technical field of magnetic resonance imaging system, especially to a conscious monkey head and coil fixing device and a magnetic resonance imaging system, the conscious monkey head and coil fixing device, including a monkey box for accommodating the monkey body, one end face of the monkey box is an upward inclined plane, a head hole for exposing the monkey head is formed in the middle part of the inclined plane, and a neck positioning module, left and right positioning modules, an upper end positioning module and a lower end positioning module are respectively arranged on the outer surface of the monkey box, through the above arrangement, the problem that the fixing device in the existing magnetic resonance imaging system is difficult to fix the head of a conscious macaque and the conscious macaque is prone to restlessness is solved.

The invention belongs to the technical field of animal model construction, and particularly relates to a spinal cord hypertension syndrome disease model modeling method and application. A guinea pig or a macaque is used as an experimental animal, and a composite biostimulation material and an accurate pressure regulation and control modeling system are combined to realize modeling. The composite biostimulation material is composed of a core pressure slow-release layer, a middle biocompatible coating and a surface targeting binding layer, has excellent biocompatibility, degradability and targeting property, and can reduce non-specific inflammatory response; the precise pressure regulation and control modeling system comprises a pressure sensing module, a micro-injection module, a real-time monitoring module and a main control module, and can dynamically regulate and control the pressure in the spinal cord in a closed-loop mode. The modeling method is easy and convenient to operate and good in repeatability, the model building success rate exceeds 95%, the pathological features are highly similar to clinical features and can last for 4 weeks, a reliable experimental model is provided for mechanism research, drug screening and therapeutic schedule evaluation of the spinal cord high pressure syndrome, and the modeling method is suitable for large-scale popularization.

The invention discloses a macaque skin squamous carcinoma cell line MCSCC14397 and application of the macaque skin squamous carcinoma cell line MCSCC14397. The macaque skin squamous carcinoma cell line is named as macaque skin squamous carcinoma cell line MCSCC14397, and the preservation number of the macaque skin squamous carcinoma cell line is CCTCC (China Center For Type Culture Collection) NO: C2025156. The cell line disclosed by the invention has similar protein expression with primary tumor tissues, has chromosome abnormal karyotype, in-vitro tumor formation capability and stable proliferation characteristics, and simultaneously shows remarkable genome instability (such as high-frequency Cgt, T mutation and structural variation) and inflammation signal activation. The MYO10 gene expression in the cell line is up-regulated and drives DNA damage reaction and inflammatory factor expression, and unique advantages are provided for application of the cell line in skin squamous cell carcinoma mechanism research, drug screening and drug efficacy evaluation. The cell line disclosed by the invention can be used as an effective cell model, can be used for establishing a xenotransplantation animal model, and can provide a research basis for deeply researching the occurrence, development, metastasis mechanism and drug resistance mechanism of human skin squamous cell carcinoma as well as innovative drug development and screening.

The application discloses a kind of based on the relevance calculation method of human face and macaque face with acne, belong to digital image processing field and depth learning field.The application designs image processing, neural network, multi-modal fusion, contour similarity calculation, data set production and acquisition.The application eliminates the individual uniqueness by enlarging data set, superimposed fusion to the face segmentation result of many people, contour fitting, explores commonality problem.The conclusion whether there is relevance between human face acne occurrence area and macaque face hair area is obtained by the similarity calculation of fitting contour.