56 results about "Macaque" patented technology

The invention relates to the field of molecular genetics, in particular to a cynomolgus monkey specific SNP site combination, a liquid chip and application of the cynomolgus monkey specific SNP site combination and the liquid chip, 1508 cynomolgus monkey specific SNP sites disclosed and screened by the invention have high polymorphism, and rapid identification, paternity identification and lifelong tracking identification of cynomolgus monkey individuals can be realized. Liquid phase chip design is carried out on the sites, chip inspection is carried out on 383 cynomolgus monkey samples from three different groups, the result shows that the average site detection rate is 99.47%, and it is indicated that the liquid phase chip has excellent site capturing efficiency. According to the method, the genetic detection capability and accuracy of the cynomolgus monkeys can be greatly improved, the genetic detection cost can be remarkably reduced, and an important technical support is provided for improving the breeding management of the cynomolgus monkeys and optimizing the population structure.

The invention provides an animal upper limb movement training device and an operation method thereof, and the device comprises a data collection module which is used for collecting behavior data of a target animal; the controller is used for generating a first control instruction according to the behavior data; the executing mechanism comprises a mechanical arm and a plurality of touch objects; the plurality of touch objects are located in a contactable range of the target animal; the mechanical arm is used for driving the target touch object to change the position and the moving track according to the first control instruction; and for each touch object, the touch object is used for sending feedback information to the controller under the condition of confirming that the target animal touches the target touch object so as to instruct the controller to allocate an award to the target animal. According to the device, the animal training task process can be adjusted in real time by collecting the macaque visual gazing information, the multi-joint point pose information and the task performance information, and the animal exercise training efficiency is improved.

The present invention provides a multi-strand multi-targeting bispecific antigen-binding molecule characterized by comprising a first and a second bispecific entity, each entity comprising a domain that binds to a target, a second domain that binds to an extracellular epitope of human and macaque CD3 epsilon chains wherein the two bispecific entities are linked to each other by a spacer, the spacer separates the first and second bispecific entities. In addition, the invention provides polynucleotides encoding the multi-targeting bispecific antigen binding molecules, vectors comprising such polynucleotides, host cells expressing constructs, and pharmaceutical compositions comprising the antigen binding molecules.

The invention belongs to the technical field of biological medicine and immunology, and particularly relates to a single-domain antibody targeting cross binding of human and cynomolgus monkey CD46 and application of the single-domain antibody. The alpaca is utilized to generate the nanoscale antibody, the molecular weight of the nanoscale antibody is about 15 kDa, the nanoscale antibody can more efficiently penetrate solid tumor tissues or cross a blood brain barrier, and the binding efficiency of the antibody and a target spot is improved as much as possible. The 11 single-domain antibodies prepared by the invention have the specificity of resisting human and cynomolgus monkey CD46, the binding potency of the 11 single-domain antibodies is remarkably superior to that of an Anti-CD46 humanization antibody in the binding detection of CD46 target protein and CD46 overexpression cells, and the 11 single-domain antibodies can be used for treating tumors. Meanwhile, the single-domain antibody disclosed by the invention has cross-species cross binding capacity, can be directly used for preclinical pharmacodynamics and safety evaluation, does not need to develop a species-specific substitution antibody, and realizes'one target and double effects'.

The invention belongs to the technical field of gardening, and discloses a test method for improving drought resistance of kiwi fruit based on trans-zeatin, which comprises the following steps: collecting germplasm resources of kiwi fruit, and breeding seedlings; carrying out natural drought treatment on the kiwi fruit rootstock seedlings, and carrying out trans-zeatin leaf spraying on the control group and the treatment group for three days before drought treatment; according to the method, morphological indexes and physiological and biochemical indexes after drought treatment are analyzed, drought resistance related index determination covers several main aspects of plant drought resistance characterization such as photosynthetic physiology, morphological adaptation, osmotic substance adjustment and antioxidant enzyme adjustment, the indexes are extremely representative, and the drought resistance of the kiwi fruit rootstock can be comprehensively and reliably represented; the external application of 50 [mu] M of trans-zeatin can significantly influence the photosynthesis, morphological growth, osmotic substance regulation and active oxygen scavenging ability of the macaque stock, thereby promoting the improvement of drought resistance.

AAV vectors are ideally suited for long-term delivery of a combination of broadly neutralizing antibodies to achieve sterilizing immunity to HIV. Unfortunately, host immune responses to the delivered antibody have severely limited the efficacy. The TLR9 pathway has been identified in initiating adaptive immune responses against AAV and delivered bNAbs. To enhance this strategy, we validated Hydroxychloroquine, a known drug inhibitor of the TLR9 pathway, as a pretreatment to AAV delivery to avoid anti-drug antibody responses. TLR9 signaling inhibition was validated using a HEK-Blue hTLR9 reporter cell line and CpG stimulation. Hydroxychloroquine was also validated in a 3-macaque trial where AAV9-3BNC117 and AAV9-10-1074 were administered along with 3 doses of hydroxychloroquine once a week starting 1 week before AAV inoculation. Unlike historical controls, where 3BNC117 and 10-1074 expression is lost within the first 4-5 weeks, 2 macaques maintained 10-1074 expression and 1 macaque maintained 3BNC117 for the duration of the trial. Anti-3BNC117 antibodies were only observed in 2 of the 3 macaques and were significantly delayed. Anti-10-1074 antibody responses were a log lower than typically observed in historic controls. The use of hydroxychloroquine as a pretreatment for AAV inoculation is a promising strategy. The significant decrease in anti-10-1074 antibody levels and the successful delivery of 10-1074 in 2 macaques and 3BNC117 in 1 macaque was very encouraging. Extending the dosage of hydroxychloroquine beyond 3 doses may be sufficient to observe long-term bNAb expression in all animals and further decrease ADA responses. Together, these data suggest that the short-term treatment of hydroxychloroquine at the time of AAV inoculation has a meaningful impact on tolerance induction to our AAV-delivered bNAbs.

The utility model discloses a helmet for macaque percutaneous acupoint electrical stimulation experiments, which comprises a helmet body, a neck ring arranged below the helmet body, an opening arranged on the helmet body at a position corresponding to the face of a macaque, an eyepiece movably arranged at the position of the opening, and a needle therapy instrument fixedly arranged on the inner side of the helmet body at a position corresponding to the top of the macaque. The needle therapy instrument is connected with a head stimulation electrode and a detection electrode, the head stimulation electrode can stimulate acupuncture points of the head of the macaque, and the detection electrode can detect brain wave signals; the needle therapy instrument is connected with the wireless communication module, and the needle therapy instrument can be remotely controlled through the wireless communication module, and changes of brain waves can be remotely received and recorded in real time. According to the utility model, the eyepiece which can be opened and closed is arranged, so that a hand can conveniently stretch into the helmet to fix the stimulating electrode and the detection electrode on the head of the macaque, and the condition that the electrodes are pulled down by the macaque in an experiment process is avoided; in addition, through double-position supporting of the neck and the head top of the macaque, the pressing feeling of the helmet body can be reduced.

The present invention discloses a device and a method for testing the exploration behavior of macaques. The device includes a base, a first telescopic rod, a support block, a second telescopic rod, a rotary motor, and a laser projection pen; a control system and a video recording system are installed on the base; the first telescopic rod is horizontally installed on the top surface of the base; the support block is installed at the telescopic end of the first telescopic rod, and the support block is slidably connected to the top surface of the base; the second telescopic rod is vertically installed on the support block, and an angle adjustment support is installed at the telescopic end of the second telescopic rod; the rotary motor is installed on the angle adjustment support, and a turntable is installed on the output shaft of the rotary motor; the laser projection pen is detachably connected to the turntable; wherein, a vibration motor is installed on the turntable, and the first telescopic rod, the second telescopic rod, the rotary motor, the laser projection pen, and the vibration motor are all electrically connected to the control system. The present invention can accurately measure the exploration behavior of animals such as macaques, is flexible in operation, has a high degree of automation, and provides convenience for data collection of the exploration behavior of macaques.

The invention discloses a macaque cytomegalovirus detection kit supporting multi-mode interpretation and a detection method, and particularly relates to the technical field of virus detection. The kit comprises a nucleic acid quick release reagent, a primer group, an isothermal amplification reaction system, a CRISPR (clustered regularly interspaced short palindromic repeats) detection system, a visual detection component, positive and negative controls, related buffer solutions, enzymes and the like. The detection method integrates nucleic acid extraction-free, ERA isothermal amplification and CRISPRCas12a technologies, realizes rapid amplification and specific detection of target virus nucleic acid, and supports three result interpretation modes of fluorescence, ultraviolet visualization and a lateral flow chromatography test strip. The method has high sensitivity and specificity, the whole process from sample treatment to result output can be completed within 50 min, complex instruments and nucleic acid purification steps are not needed, operation is easy and convenient, cost is low, and the method is suitable for on-site rapid screening of the macaque cytomegalovirus and basic-level facility application.

The invention discloses a macaque face recognition and kinship identification system based on deep learning, and relates to the technical field of face recognition. The invention comprises a dynamic capture module, a processing module, a face recognition module, a behavior analysis and kinship identification association analysis module; the dynamic capture module is used to capture macaque activity video data in an activity area; firstly, a suitable dynamic capture high-speed camera is selected to ensure that clear and continuous macaque activity video data can be captured, and cameras are arranged in the macaque activity area, the activity area is set on the macaque key area and path, and multiple cameras are arranged to cover different viewing angles and distances; the invention uses deep learning technology to quickly identify and analyze macaque facial features, thereby significantly improving the efficiency and accuracy of face recognition, and at the same time combines behavior analysis and genetic marker data to further improve the accuracy of kinship identification.

The invention discloses a biomedicine-based macaque saliva detection analyzer, and relates to the technical field of macaque saliva detection.The biomedicine-based macaque saliva detection analyzer comprises a machine body and a display screen, the display screen is arranged on the surface of the machine body, a receiver is arranged at the front end of the machine body, and the receiver detects collected saliva and displays a detection result on the display screen; the surface of the receiver is connected with an absorption pipe, clamping rods are rotatably installed on the two sides of the bottom of the machine body, an air cylinder is installed at the bottom of the machine body, macaque saliva is collected through the absorption pipe, it is ensured that a safe distance is kept between a collector and a macaque during saliva collection, and a medical sponge is used for smearing a phagostimulant to attract the macaque to actively chew; saliva secreted when the macaque chews is collected and detected in time through the absorption tube, struggling of the macaque in the saliva collection process is avoided, the personal safety of collection personnel is guaranteed, the components of the collected saliva are stable, and the accuracy of a detection result is guaranteed.

The invention discloses a biomarker C10757 for detecting a health state and application thereof, relates to the technical field of biology, and solves the technical problems that traditional psychological stress assessment depends on behavior observation (such as animal depression-like behaviors) and has the defects of strong subjectivity, low sensitivity, obvious hysteresis and the like. According to the technical scheme, the biomarker C10757 of the health status is provided, the molecular formula of the biomarker C10757 is C20H27N3O3, the health status is caused by the psychological stress generated by the wild macaque, and the application of the biomarker C10757 in preparation of a detection product for the health status caused by the psychological stress generated by the wild macaque is provided. Through the metabolic function of intestinal microorganisms, non-targeted metabonomics analysis, pathway enrichment and ROC analysis determination, the effects of accurately distinguishing individuals affected by psychological stress from normal individuals and remarkably improving the sensitivity and specificity of detection are achieved.

The utility model discloses an observation system for monitoring the plant ingestion habit of macaque, which comprises a support rod, the top end of the support rod is fixedly connected with a first monitoring probe, and the outer wall of one side of the support rod is fixedly connected with a second monitoring probe and a signal transceiver. The other side of the supporting rod is fixedly connected with two bearing plates which are symmetrically arranged, rotating grooves are formed in one sides of the two bearing plates, threaded rods are rotationally connected into the rotating grooves, the thread rotating directions of the two sides of the central point of the horizontal axial section of each threaded rod are opposite, and the two ends of each threaded rod are fixedly connected with traction plates; one end of the traction plate is fixedly connected with an arc-shaped clamping plate, a supporting frame is arranged between the two bearing plates, and the supporting frame is fixedly connected with the supporting rod. The monitoring devices at different positions can be used for observing ingestion and daily activities of macaque, and the whole device is convenient to disassemble and assemble through the arrangement of the clamping and fixing assembly; and popularization and use are facilitated.

The present application relates to the technical field of magnetic resonance imaging system, especially to a conscious monkey head and coil fixing device and a magnetic resonance imaging system, the conscious monkey head and coil fixing device, including a monkey box for accommodating the monkey body, one end face of the monkey box is an upward inclined plane, a head hole for exposing the monkey head is formed in the middle part of the inclined plane, and a neck positioning module, left and right positioning modules, an upper end positioning module and a lower end positioning module are respectively arranged on the outer surface of the monkey box, through the above arrangement, the problem that the fixing device in the existing magnetic resonance imaging system is difficult to fix the head of a conscious macaque and the conscious macaque is prone to restlessness is solved.

The present invention discloses isolated antibodies that bind to human CD38 and macaque CD38. The present invention also discloses pharmaceutical compositions containing the disclosed antibodies, and therapeutic and diagnostic methods using the disclosed antibodies.

The invention belongs to the technical field of animal model construction, and particularly relates to a spinal cord hypertension syndrome disease model modeling method and application. A guinea pig or a macaque is used as an experimental animal, and a composite biostimulation material and an accurate pressure regulation and control modeling system are combined to realize modeling. The composite biostimulation material is composed of a core pressure slow-release layer, a middle biocompatible coating and a surface targeting binding layer, has excellent biocompatibility, degradability and targeting property, and can reduce non-specific inflammatory response; the precise pressure regulation and control modeling system comprises a pressure sensing module, a micro-injection module, a real-time monitoring module and a main control module, and can dynamically regulate and control the pressure in the spinal cord in a closed-loop mode. The modeling method is easy and convenient to operate and good in repeatability, the model building success rate exceeds 95%, the pathological features are highly similar to clinical features and can last for 4 weeks, a reliable experimental model is provided for mechanism research, drug screening and therapeutic schedule evaluation of the spinal cord high pressure syndrome, and the modeling method is suitable for large-scale popularization.

The invention relates to the technical field of magnetic resonance imaging systems, in particular to a sober monkey head and coil fixing device and a magnetic resonance imaging system.The sober monkey head and coil fixing device comprises a monkey box used for containing a monkey body, one end face of the monkey box is an upward inclined face, and a head hole used for exposing a monkey head is formed in the middle of the inclined face; the neck positioning module, the left-right positioning module, the upper end positioning module and the lower end positioning module are arranged on the outer surface of the monkey box. By means of the arrangement, the problems that a fixing device in an existing magnetic resonance imaging system is difficult to fix the head of a sober macaque, and the sober macaque is prone to restlessness are solved.

Herein is reported an anti-transferrin receptor antibody that specifically binds to a human transferrin receptor and a macaque transferrin receptor, comprising i) a humanized heavy chain variable domain derived from the heavy chain variable domain of SEQ ID NO: 01, and ii) a humanized light chain variable domain derived from the light chain variable domain of SEQ ID NO: 26, wherein the antibody has a dissociation rate for the human transferrin receptor equal to or less than (i.e., at most) the dissociation rate of the anti-transferrin receptor antibody 128.1 for the macaque transferrin receptor wherein the dissociation rate is determined by surface plasmon resonance, and the anti-transferrin receptor antibody 128.1 has a heavy chain variable domain of SEQ ID NO: 64 and a light chain variable domain of SEQ ID NO: 65.

The present invention pertains to the nanoencapsulation of siRNA and other biologics in phospholipid nanosomes for the improved delivery of siRNA and other biologics to targeted diseased human or animal organs and human or animal cells and apparatus and methods for making the same. In embodiments of the present invention, novel siRNAs were designed to down regulate CCR5 and CD4, based on an analysis of all known alternative transcripts for each gene from both human and monkey (Macaca mulatta) genomes. Embodiments of the present invention feature supercritical, critical and near critical fluids. Embodiments of the present invention also pertain to down regulation of CXCR4 receptor and targeting of nanosomes containing specific siRNA sequences to cells expressing those receptors on the cell surface by coating them with specific ligands. These include ligands for the receptors CCR5, CD4 and CXCR4.

The invention provides a macaque individual identification method and system, and the method comprises the steps: obtaining a to-be-detected image containing a to-be-identified macaque individual; the face region detection model is obtained by training a preset YOLOv10 model based on a first feature curve corresponding to a macaque face region in the training macaque individual image; cutting the image data based on the target bounding box, and inputting a facial region image obtained after cutting into a trained facial feature recognition model for feature extraction and recognition processing to obtain a recognition result of the to-be-detected image; and based on the identification result, determining archive information of the corresponding macaque individuals in a macaque individual database. According to the scheme, the macaque individual identification accuracy and efficiency can be improved.

The invention relates to the technical field of medical instruments for experimental animals, and discloses an intrathecal injection dosing tool for experimental animals. The fixing device is arranged on the outer side of the needle seat; the push rod is movably connected into the needle seat; the end, away from the push rod, of the needle base is fixedly connected with the protective sleeve. The injection needle penetrates through the protective sleeve and is in sliding connection with the protective sleeve; the depth adjusting assembly is arranged in the protective sleeve, the fixing device and the depth adjusting assembly are provided, the injection needle can be more accurately inserted into the intrathecal space of the macaque, meanwhile, the adjustable fixing device and the depth adjusting assembly can adapt to macaque of different body types and ages, the universality and practicability of the tool are improved, and the tool is convenient to use. The needle base is provided with the measuring meter and the matched injector, the injection dosage of medicine can be more accurately controlled, the strict requirements of experiments for the medicine concentration and dosage are met, and the accuracy and reliability of experiment results are improved.

The application discloses a macaque detection method based on a YOLOv7 network and a Deepsort network, inputs a macaque motion video sequence into the network, extracts and processes frame images, obtains a prediction result by extracting features of the input images through the optimized YOLOv7 network, sends the prediction result into the Deepsort network again as input information to obtain a prediction frame sequence, and finally detects and processes missing values of the prediction frame sequence. The application is based on a single-stage target detection algorithm, combines a time sequence transfer module (TSM) to extract space-time information of a target video sequence, improves the precision of macaque detection, concatenates a Deepsort algorithm to the detection output position to re-process and optimize the output result, and solves the problems of difficulty in feature selection, low recognition rate, influence of factors such as brightness, contrast change and shadow in the image, and low detection precision under the influence of cage shielding.

The invention discloses a method for prolonging the spawning period of epinephelus moara, which comprises the following steps: (1) selecting juvenile epinephelus moara with the body weight of 100-150g as a parent fish rearing object, with the dissolved oxygen of 5-9mg / L, the salinity of 25-33, the pH of 7.8-8.2, the water flow exchange capacity of 2-3m < 3 > / h, the water temperature of 25-28 DEG C, the sunlight illumination time of 13.5 h and the rearing density of 6-8kg / m < 3 >; the feed is fed once a day, and the traditional Chinese medicine composition is taken for 10d / month; (2) when the parent fish reaches the sexual maturity age, the water temperature is reduced to 20 DEG C, the sunlight illumination time is 12 h, and the breeding density is 6-8 kg / m < 3 >; the feed is fed twice and three times every day, and the traditional Chinese medicine composition is taken for 10d / month; after one month, the water temperature is gradually increased to 25 DEG C, and the daily illumination time is gradually increased to 13.5 h; (3) when the gonad of the parent fish develops to 3-4 stages, injecting chorionic gonad promoting hormone for spawning induction; (4) after the parent fishes lay eggs for 30 days, the water temperature is gradually reduced to 20 DEG C, and meanwhile, the illumination time is gradually reduced to 12 hours; after 30 days, the water temperature is gradually increased to 25 DEG C, and the illumination time is gradually increased to 13.5 h. According to the method, the breeding period of the parent fish of the epinephelus moara is prolonged.

The invention provides a primate-based intelligent noninvasive brain pacemaker performance and effectiveness verification method and system, and the method and system show significant advantages in the aspects of technology, economy and experimental ethics. According to the method, high space-time resolution source imaging combining high-density EEG and magnetic resonance imaging (MR I) is adopted, the system can accurately capture TES-induced neural oscillation changes (the time resolution is 1ms) through a millisecond-level dynamic response correlation technology, and the spatial positioning accuracy smaller than or equal to 2mm is achieved in a Parkinson disease macaque model. In addition, long-term dynamic research as long as 6 months is supported, a reliable basis is provided for clinical transformation, and it is predicted that the clinical test failure rate can be reduced by 20%. The method not only accords with the '3R principle 'of animal experiments, but also provides an innovative platform with scientific value and social benefit for brain disease treatment research through non-invasive, high-precision and multi-dimensional technical breakthrough.

The present invention discloses a chimeric animal model based on hNPY gene overexpression, an engineered monkey and its application. The chimeric animal model based on gene overexpression is characterized in that the NPY gene is overexpressed in the paraventricular nucleus cell gene of the animal to induce the phenotype, thereby forming a chimeric animal model based on NPY gene overexpression. The chimeric genetic engineered monkey model is characterized in that the hNPY gene is overexpressed in the paraventricular nucleus cell gene of the macaque to induce the phenotype, thereby forming a chimeric genetic engineered monkey model based on hNPY gene overexpression. The model can be used for the study of the pathogenic mechanism of related diseases, the screening, verification, and testing of therapeutic drugs for clinical treatment methods, and related preclinical research. The present invention has the characteristics of short construction cycle of animal models, high efficiency, low cost, high phenotypic penetrance, good synchronization, high repeatability, low difficulty, and convenience for large-scale application.

The utility model discloses a macrobrachium rosenbergii breeding illumination device with uniform illumination, and relates to the technical field of macrobrachium rosenbergii breeding, the macrobrachium rosenbergii breeding illumination device comprises a seedling raising plate and a reflection assembly, the bottom of the seedling raising plate is provided with a rotating assembly, and the reflection assembly is arranged on one side of the top of the seedling raising plate. According to the uniform-illumination illumination device for macrobrachium rosenbergii breeding, through the arrangement of the reflection assembly, when a shadow is formed on one side of the interior of a seedling raising tray due to the fact that a light source and the seedling raising tray deviate, a rotating plate can be pushed, so that an external light source is reflected to the shadow through a first mirror surface, and illumination compensation is conducted on the shadow; meanwhile, a damping pad is used for increasing friction force between a rotating plate and a fixing lug, so that automatic positioning is achieved after adjustment, a fastener in a threaded sleeve can be unscrewed to be separated from an adjusting plate, and then the adjusting plate can slide in the rotating plate; and the second mirror surface can be used for adjusting the illumination width after reflection.

The invention discloses a macaque skin squamous carcinoma cell line MCSCC14397 and application of the macaque skin squamous carcinoma cell line MCSCC14397. The macaque skin squamous carcinoma cell line is named as macaque skin squamous carcinoma cell line MCSCC14397, and the preservation number of the macaque skin squamous carcinoma cell line is CCTCC (China Center For Type Culture Collection) NO: C2025156. The cell line disclosed by the invention has similar protein expression with primary tumor tissues, has chromosome abnormal karyotype, in-vitro tumor formation capability and stable proliferation characteristics, and simultaneously shows remarkable genome instability (such as high-frequency Cgt, T mutation and structural variation) and inflammation signal activation. The MYO10 gene expression in the cell line is up-regulated and drives DNA damage reaction and inflammatory factor expression, and unique advantages are provided for application of the cell line in skin squamous cell carcinoma mechanism research, drug screening and drug efficacy evaluation. The cell line disclosed by the invention can be used as an effective cell model, can be used for establishing a xenotransplantation animal model, and can provide a research basis for deeply researching the occurrence, development, metastasis mechanism and drug resistance mechanism of human skin squamous cell carcinoma as well as innovative drug development and screening.

The present disclosure provides antibodies and antigen-binding fragments thereof that specifically bind to human B7-H4 (and optionally to macaque, mouse and / or rat B7-H4), as well as compositions comprising such antibodies or antigen-binding fragments thereof. In one particular aspect, the antibody specifically binding to human B7-H4, or antigen-binding fragment thereof, increases T cell proliferation, increases interferon-gamma production, and / or depletes cells expressing B7-H4 by ADCC activity. The disclosure also provides methods of treating conditions, such as cancer, by administering an antibody or antigen-binding fragment thereof that specifically binds to human B7-H4.

The invention discloses application of a biomarker HMDB0254633 in detection of HBB gene mutation of macaque, relates to the technical field of biology, and solves the technical problem of lack of a specific biomarker for early detection of thalassemia intestinal complications caused by HBB mutation. The key points of the technical scheme are as follows: the invention provides application of the biomarker HMDB0254633 in preparation of a quantitative detection product for macaque HBB gene mutation, the chemical name of the biomarker HMDB0254633 is 3-oxooctadecanoic acid, the molecular formula of the biomarker HMDB0254633 is C18H34O3, the quantitative detection product is a reagent or a kit, and the invention further provides a method for quantitatively detecting HBB gene mutation. Comprising the following steps: 1) extracting metabolites in excrement; 2) detecting metabolites by using a quantitative detection product; the effects of providing a high-specificity non-invasive marker and a detection tool and realizing early-stage rapid and non-invasive detection are achieved.

The invention discloses sgRNA for targeted macaque ATM gene knockout and application, and belongs to the technical field of biology. The sgRNA nucleotide sequence of the targeted Macaca mulatta ATM gene provided by the invention is shown as any one of SEQ ID NO: 2, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8. The method comprises the following steps: designing a plurality of sgRNAs aiming at the ATM gene of the macaque on the basis of a CRISPR / Cas9 system, jointly injecting the sgRNA for targeted macaque ATM gene knockout and Cas9 protein or a mixture of the sgRNA for targeted macaque ATM gene knockout and Cas9 mRNA into a macaque embryo, and then transplanting the embryo into a receptor female macaque body to produce the macaque with the ATM gene knockout. The method provides a new method for establishing a non-human primate AT animal model, and provides a research basis for researching the cerebellum degenerative phenotype, pathological characteristics and internal molecular mechanism of AT patients, analyzing the pathogenic mechanism and screening drugs.