The invention discloses a protein chip used for detecting human ferrum reserve, which belongs to the field of human ferrum nutrition detection. The protein chip comprises a solid-phase carrier, antiserum ferritin monoclonal antibodies, antiserum transferrin receptor monoclonal antibodies and anti-C-reactive-protein monoclonal antibodies, wherein the antiserum ferritin monoclonal antibodies, the antiserum transferring receptor monoclonal antibodies and the anti-C-reactive-protein monoclonal antibodies are distributed on the solid-phase carrier in an array manner, wherein the monoclonal antibodies are fixed on the surface of the solid-phase carrier by covalent bonds, and the solid-phase carrier is finished by hydroformylation. The invention further discloses a detection reagent kit based on the protein chip. Compared with the prior art, the protein chip can detect three indexes of human ferrum reserve simultaneously, is accurate and reliable in results, high in detection specificity, sensitivity and detection speed and stable in detection results. Furthermore, by multi-sample microarray technology, the protein chip is applicable to screening and detecting of massive people, low in blood sample quantity, detection and cost and low in harms to examined people.