Protein chip used for detecting human ferrum reserve and reagent kit thereof

A technology of protein chip and detection of human body, which is applied in the field of protein chip, can solve the problems such as undiscovered protein chip, and achieve the effect of stable detection result, high sensitivity and simple detection method

Inactive Publication Date: 2012-08-01
中国疾病预防控制中心营养与食品安全所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, no report was found on the protein chip for detecting iron storage in human body with multiple indicators

Method used

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  • Protein chip used for detecting human ferrum reserve and reagent kit thereof
  • Protein chip used for detecting human ferrum reserve and reagent kit thereof
  • Protein chip used for detecting human ferrum reserve and reagent kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of a protein chip for detecting iron reserves in human body

[0036] Proceed as follows:

[0037] (1) Selection of solid phase carrier: choose a three-dimensional substrate in the form of an aldylated 12-lattice matrix (the crystal core polymer three-dimensional substrate, purchased from Boao Biological Co., Ltd.), and determine the arrangement of the lattice to be 3×6 array.

[0038] (2) Prepare sample solution:

[0039] a. Use 50% glycerol spotting buffer containing free Cy3 (purchased from Boao Biological Co., Ltd.) and PBS buffer of pH 7.4 according to the volume of spotting buffer: PBS: monoclonal antibody 1:1:2 Prepare respectively: mouse anti-serum ferritin monoclonal antibody (Anti-Ferritin, mitochondrial antibody [F23], abcam company) sample solution, mouse anti-soluble transferrin receptor monoclonal antibody (Anti-Transferrin Receptor antibody [ 2B6], abcam Company) sample solution and mouse anti-C reactive protein monoclonal antibody...

Embodiment 2

[0047] Example 2 A protein chip used to detect iron reserves in the human body Contrast test for detecting iron reserves in the human body

[0048] The test samples were the serum collected from voluntary subjects (20-60 years old) of adult women of childbearing age in Nanyang, Henan Province in 2010. Six cases (numbered I, II, III, IV, V, VI) were randomly selected. The sample is divided into two parts, one part is 200 μl / part, and the contents of serum ferritin, soluble transferrin receptor and C-reactive protein are detected by turbidimetry; the other part is 20 μl / part, which is used in the embodiment of the present invention 1 The prepared protein chip was used to detect serum ferritin, soluble transferrin receptor and C-reactive protein at the same time.

[0049] (1) Nephelometric test: 200 μl of serum was taken from each case and tested by turbidimetric test. Nephelometric detection was carried out according to conventional methods (Dubois S et al. GIT Labor-Medizin, 1...

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Abstract

The invention discloses a protein chip used for detecting human ferrum reserve, which belongs to the field of human ferrum nutrition detection. The protein chip comprises a solid-phase carrier, antiserum ferritin monoclonal antibodies, antiserum transferrin receptor monoclonal antibodies and anti-C-reactive-protein monoclonal antibodies, wherein the antiserum ferritin monoclonal antibodies, the antiserum transferring receptor monoclonal antibodies and the anti-C-reactive-protein monoclonal antibodies are distributed on the solid-phase carrier in an array manner, wherein the monoclonal antibodies are fixed on the surface of the solid-phase carrier by covalent bonds, and the solid-phase carrier is finished by hydroformylation. The invention further discloses a detection reagent kit based on the protein chip. Compared with the prior art, the protein chip can detect three indexes of human ferrum reserve simultaneously, is accurate and reliable in results, high in detection specificity, sensitivity and detection speed and stable in detection results. Furthermore, by multi-sample microarray technology, the protein chip is applicable to screening and detecting of massive people, low in blood sample quantity, detection and cost and low in harms to examined people.

Description

technical field [0001] The invention relates to a protein chip, in particular to a protein chip for detecting iron reserves in a human body, and a corresponding protein chip detection kit. Background technique [0002] Iron is an essential trace element in the human body. Iron deficiency can lead to reduced physical activity in adults and impaired brain development in children. Severe iron deficiency in the human body can lead to iron deficiency anemia. At present, about 2 billion people in the world are anemic, and the proportion of people with iron deficiency is even higher. Assessing the body's iron status and judging whether there is iron deficiency has important clinical significance, and it is also a technical problem that needs to be solved urgently. The selection of specific and sensitive monitoring indicators for early iron deficiency will help to detect and treat iron deficiency groups and patients with potential anemia in time, and also contribute to population...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 霍军生殷继永孙静黄建李文仙
Owner 中国疾病预防控制中心营养与食品安全所
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