79 results about "Human keratinocyte" patented technology

A skin care composition comprising at least one keratinocyte CLOCK or PER1 gene activator and at least one DNA repair enzyme; a method for inhibiting damage to human keratinocytes due to environmental aggressors by applying a composition comprising at least one keratinocyte CLOCK or PER1 gene activator and at least one DNA repair enzyme; and a method for repairing DNA damage in human keratinocytes.

Improvements are made to a novel media that replace the requirement for all protein growth factors by the addition to the medium of physiological concentrations of retinyl acetate. The media are serum-free, companion cell or feeder layer-free and organotypic, matrix free solutions for the cultivation of clonally competent basal keratinocytes. The media and methods are useful in the production of epidermal epithelial tissue that is suitable for skin grafting.

Cultures of keratinocyte cells are provided which are free from nonautologous fibroblasts and organ extracts, and which have a high speed of cell amplification for a minimum seeding density. The cultures can be cryopreserved in a buffered isotonic medium containing serum and a cryoprotectant. The cultures are produced by a process that does not involve the use of a feeder layer and organ extracts. A culture medium which can be used contains Medium 199, serum, epidermal growth factor, cholera toxin and/or hydrocortisone, and optionally insulin. A substance for wound healing and for cosmetic applications is derived from cultured human keratinocytes. A non-viable total keratinocyte lysate for use in promoting wound healing is produced by growing keratinocyte cells on a support, detaching the cells from the support, and lysing the detached cells to obtain the lysate which may be frozen and lyophilized. The cells may be grown without using a support to produce the lysate, or to produce a non-viable keratinocyte cell culture lyophilisate or spray dried non-viable keratinocyte cell composition for use in healing wounds.

Clinical performance of currently available human skin equivalents is limited by failure to develop perfusion. To address this problem we have developed a method of endothelial cell transplantation that promotes vascularization of human skin equivalents in vivo. Living skin equivalents were constructed by sequentially seeding the apical and basal surfaces of acellular dermis with cultured human keratinocytes and Bcl-2 transduced HUVEC or umbilical cord cells sequentially. After orthotopic implantation of grafts comprising cultured human keratinocytes and Bcl-2 transduced HUVEC cells onto mice, the grafts displayed both a differentiated human epidermis and perfusion through the HUVEC-lined microvessels. These vessels, which showed evidence of progressive maturation, accelerated the rate of graft vascularization. Successful transplantation of such vascularized human skin equivalents should enhance clinical utility, especially in recipients with impaired angiogenesis.

The present invention relates to a method for determining the state of epidermal differentiation of a human keratinocyte in a human epithelium sample, said method including determining the level of expression of at least one microRNA selected from among hsa-miR-455-3p, hsa-miR-141, hsa-miR-148a, hsa-miR-182, hsa-miR-224, hsa-miR-26a, hsa-miR-26b, hsa-miR-361-5p, hsa-miR-425, hsa-miR-92b, hsa-let-7i, hsa-miR-22, hsa-miR-221, hsa-miR-222, hsa-miR-29a, hsa-miR-29b, hsa-miR-663, hsa-miR-30a, and hsa-miR-30c within said keratinocyte, and comparing the level of expression of the microRNA(s) selected at the mean level of expression of the microRNA(s) selected in the undifferentiated keratinocytes or in differentiated epidermal keratinocytes, preferably from the same strain or the same population. The present invention also relates to various uses of the method for determining the state of epidermal differentiation, as well as to pharmaceutical or cosmetic compositions.

The invention belongs to the technical field of daily cosmetics, and discloses an anti-oxidation, anti-glycation and anti-aging skin care composition and an application thereof. The composition mainlycomprises carnosine, ultra-low molecular weight dendrobium officinale polysaccharide extract and bifida ferment lysate, and also can comprise a solvent and a preservative. By golden proportioning andcombined use of three components including the carnosine, the ultra-low molecular weight dendrobium officinale polysaccharide extract and the bifida ferment lysate, the synergistic effect is achieved, so that the composition has the effects of resisting oxidation, resisting glycosylation, inhibiting release of inflammatory factors by human keratinocytes after photodamage, promoting expression ofbarrier repair genes and promoting synthesis of collagen, and takes the comprehensive anti-ageing effect. In addition, the anti-oxidation, anti-glycation and anti-aging skin care composition is simplein component type and easy to prepare and easy to mix with other components in the cosmetics, and has the obvious advantage of being easy to add in various types of formulas.

The present invention relates to a cosmetic composition comprising a meteorite extract. The present invention also relates to methods of using the extract as a cosmetic agent suitable for stimulating the differentiation of human keratinocytes.

The present invention relates to an ex vivo method for obtaining a population of human keratinocytes derived from human pluripotent stem cells comprising a step of co-culturing human pluripotent stem cells with cells that support ectodermal differentiation in presence of an agent that stimulates epidermal induction and a agent that stimulates terminal differentiation of keratinocytes. A further object of the invention relates to a method for preparing a human skin substitute comprising a step of providing an organotypic culture of the substantially pure homogenous population of human keratinocytes derived from human pluripotent stem cells obtained according to the method of the invention.

The invention relates to the field of daily chemicals and plant extracts, in particular to a ginseng flower composite fermentation product and an application thereof. A ginseng flower extract is fermented by yeast and lactic acid bacteria successively, and the contents of rare ginsenosides s-Rg2, R-Rg2, S-Rg3, R-Rg3, PPT, F4, Rh4, Rg5 and Compound K in the ginseng flower composite fermentation product contained in a supernatant of the fermentation product are all increased. The ginseng flower composite fermentation product can promote human keratinocyte proliferation, inhibit tyrosinase activity, limit melanin generation, improve skin pigmentation, and has whitening effect. Therefore, the ginseng flower composite fermentation product can be used for preparing skin external preparations, and can be used as an active ingredient for skin repair, improvement of pigmentation or whitening.

It has been discovered that vascular endothelial growth factor (“VEGF”) promotes migration of activated (but not differentiating) keratinocytes to skin. This growth factor specifically increases migration of keratinocytes of the “wounded skin” phenotype but does not have significant effects upon differentiated keratinocytes. It also increases collagen deposition and reduces wrinkles, enhances skin quality, and increases skin thickness to normal levels in individuals where skin has thinned due to age or disorder such as diabetes. It is particularly well suited for use as cosmeceuticals when applied in purified form and in known amounts. The data presented in the examples demonstrate efficacy and specificity of VEGF in enhancing migration of normal human keratinocytes as well as formation of new granulation tissue including collagen formation. VEGF induces keratinocyte and fibroblast migration, formation of new tissue, and not only induces deposition of collagen but improves alignment of the collagen fibers. Accordingly, this growth factor is highly suitable for use as a cosmeceutical, especially for skin resurfacing and reduction in wrinkles.

The invention relates to methods for isolating and culturing human keratinocytes from embryonic stem cells. The methods are useful for producing substantially pure cultures of keratinocytes.

The invention relates to a quick construction preparation method of human epidermal tissues. In terms of the structure characteristics of human epidermal tissues, chitosan, gelatin and human keratinocyte are adopted for cell culture, and are subjected to film shaping, nutrient solution immersion and tissue construction to generate human epidermal tissues of the human keratinocyte under a high-pressure sterile state. The preparation method is advanced in process, the preparation efficiency and speed of human epidermal tissues can be effectively improved due to construction and use of a gas pressure device, and the human epidermal tissue can be used for skin-grafting treatment of burnt patients, and the effect of skin grafting can be improved, so that the quick construction preparation method of human epidermal tissues is perfect.

A composition based on labdane-diterpenoids that provides better photo protection against both UV A and UV B radiations in the HaCaT human keratinocyte cell lines is disclosed. The composition comprises 8,13-epoxy-1α,6β,7β,9α-tetrahydroxylabd-14-en-11-one alone and in combination with 6β-acetoxy-8,13-epoxy-1α,7β,9α-trihydroxylabd-14-en-11-one and/or 7β-acetoxy-8,13-epoxy-1α,6β,9α-trihydroxylabd-14-en-11-one. The composition protects the cells of the skin from harmful UV A and UV B rays through synergistic mechanisms with utility as a safe long-term cosmetic solution for preventing UV-induced skin damage and to induce sunless tanning. Additionally, the composition enhances melanogenesis in B16F1 mouse melanoma cells acting as tanning inducers/accelerators both in the presence or absence of sunlight. Elevation of melanogenesis confers additional protection against UV-induced skin damage.

The invention discloses a sargassum fusiforme polypeptide with a wound healing promoting effect and application thereof. The sargassum fusiforme polypeptide has the sugar content of 68-83.5%, the protein content of 1.5-12.3% and the uronic acid content of 6.4-22%. The application of the sargassum fusiforme polypeptide refers to the application in preparing medicines and functional cosmetics with the wound healing promoting effect, the proliferation of human keratinocytes can be promoted, the polypeptide has an important effect of reconstructing the after-skin-wound skin barrier function, the migration of the human keratinocytes can be promoted, and the polypeptide has the effects of accelerating the re-epithelization process and promoting the skin wound healing.

The invention discloses a Phellinus igniarius polysaccharide extract, a preparation method and applications thereof, and a skin external application agent containing the Phellinus igniarius polysaccharide extract. According to the present invention, the Phellinus igniarius polysaccharide extract can significantly clear free radicals, can promote the proliferation of human fibroblasts and the synthesis of type I collagen, can resist human keratinocyte oxidative damage, and can be used in the fields of cosmetics, health food and medicine, especially in cosmetics.

The invention relates to a preparation method of umbilical cord mesenchymal stem cell liquid for beautifying and maintenance, and belongs to the technical field of medical beautifying. The method comprises the following steps of 1, obtaining umbilical cord stem cells; 2, conducting subculture; 3, preparing the umbilical cord stem cell liquid. According to the technical scheme, umbilical cord stemcells can secrete various cell growth factors to provide a good microenvironment for skin epidermal cells, wherein the cell growth factors include the fibroblastoma growth factor, the fibroblast growth factor, the hepatocyte growth factor, the insulin growth factor, angiogenin and the like; the in-vitro proliferation and migration of human keratinocytes can be activated, skin regeneration and skinwound healing are promoted when the factors enter the skin, the maturity of the skin epidermal cells can be promoted, the skin elasticity is improved, the skin is moisturized, wrinkles are eliminated, pigmentation is prevented, and the liquid has a good beautifying effect.

An embodiment of the invention discloses a trichophyton rubrum infection model preparation method and application. The preparation method includes the steps: separating a corium layer and a cuticularlayer of prepuce tissues, and digesting and culturing the cuticular layer to obtain human keratinocytes; finally, adding the human keratinocytes to a collagen scaffold and culturing the human keratinocytes to form a tissue engineering skin model. The model is simple to operate and easy to observe and solves the problem that a current trichophyton rubrum infection animal model is not easily observed. Seeds of the trichophyton rubrum infection model are the human keratinocytes and closer to the biological state of human skin fungal infection.

The invention discloses a coupling matter formed by chemical modification of polyethylene glycol of a deletion-recombination human keratinocyte growth factor I type. The coupling matter is characterized in that covalent chemical coupling is performed on activated polyethylene glycol molecules with the molecular weight being 5K to 40K and free sulfhydryl in 79-site cysteine of the deletion-recombination human keratinocyte growth factor I type (delta 23KGF-I) with the deletion of 23-site amino acid at the N terminal, and then column chromatography purification is performed to obtain the pegylation deletion-recombination human keratinocyte growth factor I type. The coupling matter retains the biological effects of human keratinocyte growth factors, changes the internal metabolic dynamics characteristic of the factors and has values of prevention and therapy on digestive tract mucosa damage or ulcerative mucosal inflammation caused by radiotherapy and chemotherapy as well as on acute chemical liver damage.

The invention discloses a recombinant human keratinocyte factor KGF-2 (keratinocyte growth factor-2) environment-sensitive eye transfer system and application thereof, and particularly relates to an environment-sensitive eye transfer system taking KGF-2 silicon plastid as a core and a preparation method and application thereof, and the transfer system comprises the following components by weight: 1.2-3.5 wt% of KFG-2, 1-10wt% of organic - inorganic compound lipid, 6-20wt% of a protective agent and 1-5wt% of substrate gel. The KGF-2 silicon plastid is prepared by thin film hydration ultrasound, and further the environment sensitive eye transfer system is prepared. Compared with the prior art, the product can obviously improve the stability of the KGF-2 and prolong the retention time of the KGF-2 in the eye, is conducive to the absorption and utilization of the KGF-2, has good repairing effects on various corneal ulcers, also can moisten the eyeball and alleviate the dry eye symptoms, has good relieving effects on eye fatigues, and also is suitable for the repairing of other oral mucosas and mucosa trauma and the repairing after skin beauty treatment.

A chimeric skin comprising immortalized human keratinocyte cells cocultured with donor keratinocytes is disclosed.

The invention provides a method for detecting recombinant human keratinocyte growth factors (rhKGF-1). The method includes steps of 1), cultivating keratinocytes; 2) inoculating the keratinocytes and controlling the density of the keratinocytes; 3), treating rhKGF-1 samples, carrying gradient dilution on the concentration of the rhKGF-1 samples and adding 5-acetenyl-2'-deoxyuridine (EdU) into the rhKGF-1 samples with various rhKGF-1 concentration dilution degrees to prepare loading samples with the series of rhKGF-1 concentration dilution degrees; 4), washing the keratinocytes in keratinocyte plates obtained at the step 2) and removing cultivation media; 5), loading the loading samples obtained at the step 3) into keratinocyte cultivation plates obtained at the step 4) and cultivating and incubating the keratinocytes; 6), solidifying and permeabilizing the keratinocytes, and then carrying out 'Click-iT' reaction on the EdU; 7), arranging the keratinocytes treated at the step 6) in a multifunctional microplate reader, reading the fluorescence intensity (RFU) and computing the biological activity of the rhKGF-1. The method has the advantages of low detection background, high sensitivity, specificity and accuracy, good repeatability and the like. Besides, the method is particularly applicable to detecting the biological activity of the rhKGF-1 and establishing quality standards.

Disclosed is a simple method of determining SPF based on the staining pattern of viable cells like mouse fibroblasts and human keratinocytes after exposure to ultraviolet radiations. The method provides results comparable to the standard methods of SPF measurement using human subjects.