Water-soluble microemulsion and preparation method thereof

A microemulsion, water-soluble technology, applied in pharmaceutical formulations, allergic diseases, cosmetic preparations, etc., can solve the problems of difficult to achieve effective skin concentration, unfavorable skin penetration and absorption, complex active molecular structure, etc. Acidase activity, broad application prospects, stable properties

Inactive Publication Date: 2021-04-20
云南银橙汉普生命科学研究股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In general, the active molecular structure in cosmetics is relatively complex, the solubility is small, and it is difficult to reach the effective concentration of the skin.
CN201310068002.X discloses a cannabinoid compound microemulsion and its preparation method, but the existence of the oil phase in the prior art makes the particle size of the microemulsion larger, which is not conducive to the penetration and absorption of the skin

Method used

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  • Water-soluble microemulsion and preparation method thereof
  • Water-soluble microemulsion and preparation method thereof
  • Water-soluble microemulsion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 prepares the method for water-soluble microemulsion

[0047] It specifically includes the following steps: weigh and take each component: 1g industrial hemp full-spectrum oil (provided by Yunnan Litu Ruima Biotechnology Co., Ltd., RM005), 7g PPG-13-decyltetradeceth-24, 7g PEG / PPG / polybutylene glycol-8 / 5 / 3 glycerol for later use; add 0.4g ethanol to the full-spectrum industrial hemp oil, stir at 65°C until fully dissolved; then add PPG-13- to it at 65°C Decyl tetradeceth-24 and PEG / PPG / polytetramethylene glycol-8 / 5 / 3 glycerin, 600rpm, stirred until uniform, to obtain a clear and transparent self-microemulsion; under natural cooling conditions, the clarified self-microemulsion The microemulsion liquid is slowly added dropwise into ultrapure water, stirred slowly and fully dissolved to form a transparent microemulsion.

[0048] The transparent microemulsion prepared by the present invention has the following characteristics: (1) is used in cosmetics, so that...

Embodiment 2

[0049] Embodiment 2 Water-soluble microemulsion is to the detection of the inhibitory ability of hyaluronidase activity

[0050]The hyaluronidase inhibition test is the most typical in vitro method for evaluating anti-allergic activity. The anti-allergic activity of the substance is evaluated by the hyaluronidase inhibition rate. The greater the hyaluronidase inhibition rate, the stronger the anti-allergic activity. In the present invention, an in vitro hyaluronidase inhibition experiment is carried out by an improved Elson-Morgan method to evaluate the antisensitivity effect of the water-soluble microemulsion of the present invention. The specific steps are as follows: take 0.1mL of 2.5M CaCl 2 Aqueous solution and 0.5mL hyaluronidase solution (500U / mL, using acetate buffer as solvent) were incubated at 37°C for 20min; %, 2wt%) sample solution 0.5mL, or 0.5mL deionized water as a positive control, continue to incubate at 37°C for 20min; add 0.5mL sodium hyaluronate solution ...

Embodiment 3

[0052] Example 3 Detection of the influence of water-soluble microemulsion on the secretion of human keratinocyte IL-8 and IL-10

[0053] HaCaT was incubated with RPMI1640 containing 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin at 37°C and 5% CO. 2 cultured under saturated humidity conditions. Cells in the logarithmic growth phase were selected and inoculated in a 96-well culture plate. After the cells were completely attached to the wall after 24 hours of culture, the medium was replaced with RPMI 1640 containing different concentrations of industrial hemp full-spectrum oil test samples, incubated for 12 hours, and the cell culture supernatant was collected. The concentrations of IL-8 and IL-10 in the supernatant were detected with human IL-8 and IL-10 ELISA kits. see attached result Figure 4 and attached Figure 5 .

[0054] It can be seen from the figure that the water-soluble microemulsions with different industrial hemp full-spectrum oil co...

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Abstract

The invention discloses a water-soluble microemulsion. The water-soluble microemulsion is characterized in that the microemulsion comprises cannabidiol, a surfactant, a cosurfactant and water, and comprises the following components in percentage by weight of 0.5-5wt% of industrial hemp full-spectrum oil, 2 to 40 wt% of PPG-13-decyl tetradecyl alcohol polyether-24; 2-40 wt% of PEG / PPG / polybutylene glycol-8 / 5 / 3 glycerol, and the balance of water. The microemulsion disclosed by the invention is clear and transparent in color, is uniformly distributed into liquid drops with the particle size of about 10-30nm, is good in thermodynamic stability, and is not layered after being stored for a long time. Meanwhile, the microemulsion can effectively inhibit hyaluronidase activity, inhibit secretion of human keratinocyte IL-8 and promote generation of IL-10, remarkably inhibit growth of staphylococcus aureus and propionibacterium acnes and remove free radicals, and does not generate obvious blood coagulation or hemolysis reaction, so that inflammatory response of skin is relieved, and anti-inflammatory and soothing effects are achieved.

Description

technical field [0001] The invention belongs to the technical field of daily cosmetics, and more specifically relates to a water-soluble microemulsion and a preparation method thereof. Background technique [0002] The skin acts as the first line of defense of the innate immune system, and one of its main functions is to protect the body from external factors. However, with the development of society and the deterioration of the ecological environment, the challenges to the skin are also increasing. For example, the raging mosquitoes or a large amount of dust can easily bring redness and swelling to people's skin, and even cause dermatitis, allergies and localized hemorrhage and necrosis in severe cases, thereby enriching the skin surface with bacteria. [0003] Cannabidiol (CBD) is one of the main components of cannabinoids. It has no side effects such as addiction and hallucinations of Tetra-HydroCannabinol (THC). , anti-tumor and other functions, and its pharmacology is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/92A61K8/34A61K8/06A61K8/86A61P31/04A61P37/08A61Q17/00A61Q19/08
Inventor 赵娜
Owner 云南银橙汉普生命科学研究股份有限公司
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