Preparation method of umbilical cord mesenchymal stem cell liquid for beautifying and maintenance
A technology of umbilical cord stem cells and mesenchymal stem cells, which is applied in the field of preparation of umbilical cord mesenchymal stem cell liquid, can solve the problems of not being able to effectively promote the metabolism of epidermal cells, having no biologically active substances, and many harmful substances remaining, so as to promote skin wound healing , prevent stains, and easy to operate
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Embodiment 1
[0024] The invention provides a method for preparing an umbilical cord mesenchymal stem cell liquid for cosmetic maintenance, comprising the following steps:
[0025] Step 1: Obtain umbilical cord stem cells, obtain umbilical cord dermal tissue and umbilical cord blood, wash 2 to 5 times with normal saline at room temperature, cut the umbilical cord dermal tissue into small pieces, add phosphate buffered saline to the small pieces and mix, Centrifuge to remove the lower liquid, add umbilical cord blood, wash with phosphate buffered saline solution and centrifuge again, the centrifugation speed is 800rpm, centrifugation time is 5min, remove the supernatant, add collagenase, the collagenase in this step is 0.1% type Ⅰ collagenase , placed in a constant temperature shaking box at 37°C for 60 minutes, centrifuged again to remove the supernatant, then added DMEM culture solution containing fetal bovine serum, filtered through a cell sieve, centrifuged to remove the supernatant, and ...
Embodiment 2
[0030] The invention provides a method for preparing an umbilical cord mesenchymal stem cell liquid for cosmetic maintenance, comprising the following steps:
[0031] Step 1: Obtain umbilical cord stem cells, obtain umbilical cord dermal tissue, wash 4 times with normal saline at room temperature, cut the umbilical cord dermal tissue into small pieces, add phosphate buffered saline solution to the small pieces, centrifuge at room temperature to remove the lower liquid, add Umbilical cord blood was washed with phosphate buffered saline solution and then centrifuged again at a centrifugation speed of 1000rpm for 3 minutes. The supernatant was removed and collagenase was added. In this step, the collagenase was 0.1% type Ⅰ collagenase and placed at a constant temperature of 37°C. 30min in the shaking box, centrifuged again to remove the supernatant, then added the DMEM culture solution containing fetal bovine serum, filtered through a cell sieve and centrifuged to remove the super...
Embodiment 3
[0035] The invention provides a method for preparing an umbilical cord mesenchymal stem cell liquid for cosmetic maintenance, comprising the following steps:
[0036]Step 1: Obtain umbilical cord stem cells, obtain umbilical cord dermal tissue, wash 2 to 5 times with normal saline at room temperature, cut the umbilical cord dermal tissue into small pieces, add phosphate buffered saline solution to the small pieces, and centrifuge at room temperature to remove the lower layer Liquid, add umbilical cord blood, rinse with phosphate buffered saline and then centrifuge again, the centrifugation speed is 1500rpm, centrifugation time is 3min, remove the supernatant, add collagenase, the collagenase in this step is 0.1% type Ⅰ collagenase, place in Place in a constant temperature shaking box at 37°C for 20 minutes, centrifuge again to remove the supernatant, then add the DMEM culture solution containing fetal bovine serum, filter through a cell sieve, remove the supernatant by centrifu...
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