Ginseng flower composite fermentation product, preparation method and application thereof

A technology of composite fermented products and ginseng flowers, which can be applied in pharmaceutical formulations, cosmetic preparations, dressing preparations, etc., can solve the problems that rare ginsenosides cannot be produced and the content of rare ginsenosides cannot be increased much, and achieve good application prospects. Significant whitening effect and effect of improving conversion rate

Active Publication Date: 2019-03-19
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing methods, the content of rare ginsenosides is n

Method used

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  • Ginseng flower composite fermentation product, preparation method and application thereof
  • Ginseng flower composite fermentation product, preparation method and application thereof
  • Ginseng flower composite fermentation product, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 ginseng flower composite fermentation product

[0027] (1) Prepare PBD culture medium, and add ginseng flower extract, so that the concentration of ginseng flower extract is 10g / L; cool after high temperature and high pressure sterilization; in the used ginseng flower extract, the total saponin content is 15%~30% , which contains ginsenoside Re 7% to 15%, Rg1 0.5% to 2%, Rd 2% to 10%. The total sugar content is 30%-60%, and the total polyphenol content is 0.5%-2%.

[0028] (2) After activation, purification and expansion, the yeasts grown to the logarithmic growth phase were inoculated into the medium containing ginseng flower extract, and the inoculation volume was 5 mL / L.

[0029] (3) Cultivate at constant temperature on a shaking table, cultivate at 34°C for 5 days, add MRS medium suitable for the growth of lactic acid bacteria after fermentation, and cool after high temperature and high pressure sterilization;

[0030] (3) Lactic ac...

Embodiment 2

[0041] Embodiment 2 human keratinocyte proliferation experiment

[0042] Keratinocytes are the most important cells in the epidermis, participate in the formation of the physical barrier of the skin, prevent the invasion of physical, chemical and microbial and other adverse factors, and protect the skin. One of the main characteristics of skin aging is the thinning of the epidermis and the slowing of wound healing, which is mainly due to the decline in the regeneration ability of keratinocytes in the epidermis and the decrease in the proliferation ability of the keratinocyte system.

[0043]After the samples before and after fermentation were sterilized by filtration, they were added to human keratinocyte culture medium (final concentration 0.05%), and deionized water without samples was used as a blank control. After culturing for 48 hours, the cells were stained with the MTT method, and the absorbance at 550 nm was measured with a microplate reader. The proliferation rate of...

Embodiment 3

[0048] Embodiment 3 human keratinocyte scratch test

[0049] The scratch test is the most commonly used model for testing wound healing in vitro, which effectively simulates the process of cell stretching, proliferation, and migration covering the wound in the initial stage of wound healing. Therefore, this model was chosen to observe the effect of ginseng flower on the scratch repair ability of the epidermis before and after fermentation. Human primary keratinocytes were inoculated in a 6-well plate, and the cells were confluent and then scratched. At the same time, samples before and after fermentation (0.05%) were added or not added, and the cells were fixed after 24 hours and stained with Giemsa stain Observe, take pictures and record the healing of the scratches, and use the 0h scratches as a control, the results are as follows figure 1 shown.

[0050] Depend on figure 1 The results showed that when human primary keratinocytes were treated with ginseng flower fermentat...

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Abstract

The invention relates to the field of daily chemicals and plant extracts, in particular to a ginseng flower composite fermentation product and an application thereof. A ginseng flower extract is fermented by yeast and lactic acid bacteria successively, and the contents of rare ginsenosides s-Rg2, R-Rg2, S-Rg3, R-Rg3, PPT, F4, Rh4, Rg5 and Compound K in the ginseng flower composite fermentation product contained in a supernatant of the fermentation product are all increased. The ginseng flower composite fermentation product can promote human keratinocyte proliferation, inhibit tyrosinase activity, limit melanin generation, improve skin pigmentation, and has whitening effect. Therefore, the ginseng flower composite fermentation product can be used for preparing skin external preparations, and can be used as an active ingredient for skin repair, improvement of pigmentation or whitening.

Description

technical field [0001] The invention relates to the fields of daily chemicals and plant extracts, in particular to a compound fermented product of ginseng flowers and its application. Background technique [0002] The skin is an important organ of the human body. It covers the entire human body and is in direct contact with the external environment. In addition to protecting the body from various external factors, the skin is also a mirror that reflects the health of the whole body. Skin is composed of epidermis, dermis, and subcutaneous tissue, and contains skin appendages, abundant nerves, blood vessels, lymphatic vessels, and muscles. [0003] Keratinocytes are the most important cells in the epidermis, participate in the formation of the physical barrier of the skin, prevent the invasion of physical, chemical and microbial and other adverse factors, and protect the skin. One of the main characteristics of skin aging is the thinning of the epidermis and the slowing of wo...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/99A61K8/9728A61Q19/02A61Q19/08A61Q19/00
CPCA61Q19/00A61Q19/02A61Q19/08A61K8/97A61K8/99A61K2800/85A61K2800/10
Inventor 章漳李俊蒋耀权周春霞史晓婷河野善行
Owner JALA GROUP CORPORATION
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