Phellinus igniarius polysaccharide extract, preparation method and applications thereof, and skin external application agent containing Phellinus igniarius polysaccharide extract

A skin external preparation, the technology of Phellinus linteus polysaccharide, applied in the field of Phellinus linteus polysaccharide extract and its preparation, can solve the problems of low safety, poor anti-aging and anti-oxidative activities and the like

Inactive Publication Date: 2017-12-22
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide Phellinus polysaccharide extract, preparation method, application and ski...

Method used

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  • Phellinus igniarius polysaccharide extract, preparation method and applications thereof, and skin external application agent containing Phellinus igniarius polysaccharide extract
  • Phellinus igniarius polysaccharide extract, preparation method and applications thereof, and skin external application agent containing Phellinus igniarius polysaccharide extract
  • Phellinus igniarius polysaccharide extract, preparation method and applications thereof, and skin external application agent containing Phellinus igniarius polysaccharide extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of Phellinus Phellinus Polysaccharide Extract

[0042] Step 1: Submerge 100g of Sanghuangzi powder (the particle size of Sanghuangzi powder is 60 mesh) with 1000ml of water, extract in boiling water for 1 hour, extract 3 times, separate solid-liquid after each extraction to obtain filtrate, combine the filtrate and concentrate To a minimum volume of 100ml, add 300ml of ethanol with a volume concentration of 95%, let stand at 4°C for 8 hours, centrifuge, collect the supernatant, and evaporate the ethanol to obtain a precipitate of 2.7g;

[0043] Step 2: After redissolving the precipitate with 100ml water, add 100ml 30% w / v trichloroacetic acid aqueous solution, deproteinize at 4°C for 3 hours, centrifuge, and neutralize the supernatant with 10% w / v NaOH aqueous solution until Neutral, dialyzed with running water for 2 days to obtain the liquid in the dialysis bag, which is concentrated to 50ml;

[0044] Step 3: Add 150mL of ethanol with a volume co...

Embodiment 2

[0045] Embodiment 2 Phellinus Phellinus Polysaccharide Extract Active Components Physicochemical Test

[0046] Determination of total sugar content

[0047] The standard glucose and the sample to be tested were dissolved in water. Take 0.2 mL of glucose standard solution or sample solution to be tested, add 0.4 mL of 5% phenol, shake, add 2.0 mL of concentrated sulfuric acid, shake, place at room temperature for 30 min, and measure the OD value at 490 nm with a blank tube as a control. Make a standard curve from the OD490 of each concentration of the standard solution against the glucose content, and calculate the total sugar content in the sample according to the standard curve.

[0048] Determination of acidic polysaccharide content

[0049] The standard glucuronic acid and the sample to be tested were dissolved in water. Take 0.4 mL of glucuronic acid standard solution or sample solution to be tested, put it in ice bath, add 2.4 mL of reagent A (0.0125 mol / L sodium tetra...

Embodiment 3

[0053] Example 3 Research on the free radical scavenging effect of Phellinus polysaccharide extract

[0054] 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen-centered organic radical. The DPPH method was proposed in 1958 and is widely used in the quantitative determination of the anti-aging ability of biological samples, classified substances and food. This method is based on the fact that the DPPH radical has a single electron and has a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, its absorption gradually disappears due to pairing with its single electron, and its fading degree has a quantitative relationship with the number of electrons it accepts. Therefore, a spectrophotometer can be used for rapid quantitative analysis to detect free radical scavenging. , so as to evaluate the anti-aging ability of the samples.

[0055] Take the Phellinus polysaccharide extract prepared in Example 1 and use dei...

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Abstract

The invention discloses a Phellinus igniarius polysaccharide extract, a preparation method and applications thereof, and a skin external application agent containing the Phellinus igniarius polysaccharide extract. According to the present invention, the Phellinus igniarius polysaccharide extract can significantly clear free radicals, can promote the proliferation of human fibroblasts and the synthesis of type I collagen, can resist human keratinocyte oxidative damage, and can be used in the fields of cosmetics, health food and medicine, especially in cosmetics.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a Phellinus polysaccharide extract and its preparation method and application. Background technique [0002] In 1956, Harman proposed the theory of free radical aging [Harman, D. Aging: A theory based on free radical and radiation chemistry. J. Gerontol. 1956, 11:289-300.], thinking that excessive free radicals are the cause of biological aging important reason. According to this theory, excess active oxygen free radicals in the body react with unsaturated fatty acids to produce substances such as malondialdehyde, and malondialdehyde will react with proteins on the cell membrane to produce brown pigment, which precipitates on the skin and becomes various stains. Excessive free radicals can also cause cross-linking and denaturation of collagen fibers and elastic fibers in the skin, making them brittle and losing elasticity. When the skin is insufficiently hydrated, it is easy t...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K8/73A61Q19/08A61Q19/02A61K31/715A61P17/18A61P17/00
CPCA61K8/73A61K31/715A61K36/06A61Q19/02A61Q19/08C08B37/0003
Inventor 章漳李慧周春霞沈忠海席淑云杨允芸
Owner JALA GROUP CORPORATION
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