Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quick construction preparation method of human epidermal tissues

A technology for epidermal tissue and human skin, applied in the field of rapid construction and preparation of human epidermal tissue, can solve the problems of long time, the requirement and time for rapid wound closure of clinical treatment, and limited autologous skin donors.

Inactive Publication Date: 2014-04-16
RES INST OF NANJING RUNNAN MEDICAL ELECTRONICS CO LTD
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Scar patients are a huge group, especially skin burns. Patients with small skin burns mostly use autologous skin transplantation. For patients with large deep skin burns, autologous skin donors are limited. It is necessary to construct an epidermal structure similar to the human body in vitro to treat skin burn patients. Effective; there are many forms of in vitro epidermal reconstruction methods, such as Genzyme's epidermal membrane and epidermal substitutes; however, due to the strict in vitro culture conditions of human epidermal tissue and the long time, it often fails to meet the requirements of clinical treatment for rapid wound closure and time, so it is necessary to find a method for quickly constructing and preparing human epidermal tissue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quick construction preparation method of human epidermal tissues
  • Quick construction preparation method of human epidermal tissues
  • Quick construction preparation method of human epidermal tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0106] The present invention will be further described below in conjunction with accompanying drawing:

[0107] figure 1 As shown in the figure, it is a state diagram for the rapid construction and preparation of human epidermal tissue. The position and connection relationship of each part must be correct, the installation should be firm, the proportion should be proportioned, and the operations should be performed in sequence.

[0108] Quantities of chemicals used in preparation are determined in pre-set ranges in grams, milliliters, millimeters, centimeters 3 is the unit of measurement.

[0109] The rapid construction and preparation of human epidermal tissue is carried out in an autoclave under gas pressure, at 37°C, under a pressure of 3.4KPa, and in a sterile state;

[0110] The autoclave is rectangular, the lower part of the autoclave 1 is an electric control box 18, and the upper part is a high-pressure sealed kettle cover 2; the inner bottom of the autoclave 1 is ste...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a quick construction preparation method of human epidermal tissues. In terms of the structure characteristics of human epidermal tissues, chitosan, gelatin and human keratinocyte are adopted for cell culture, and are subjected to film shaping, nutrient solution immersion and tissue construction to generate human epidermal tissues of the human keratinocyte under a high-pressure sterile state. The preparation method is advanced in process, the preparation efficiency and speed of human epidermal tissues can be effectively improved due to construction and use of a gas pressure device, and the human epidermal tissue can be used for skin-grafting treatment of burnt patients, and the effect of skin grafting can be improved, so that the quick construction preparation method of human epidermal tissues is perfect.

Description

technical field [0001] The invention relates to a rapid construction and preparation method of human epidermal tissue, which belongs to the technical field of construction, preparation and application of biological tissue materials. Background technique [0002] The skin covers the surface of the human body and is the largest organ of the human body. As the first physiological defense line of the human body, the skin is always involved in the functional activities of the body, maintains the metabolic balance in the body through secretion, excretion and absorption, and protects the internal tissues and organs External environmental damage; human skin tissue is composed of epidermis, dermis and subcutaneous tissue. The human epidermis plays an important role in the repair of skin trauma. If the wound cannot heal in time, it will often cause loss of water and protein, increase the chance of infection, and are very easy to Hypertrophic scars are formed, and if the epidermis ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/26
Inventor 安美文刘阳王立陈凌峰
Owner RES INST OF NANJING RUNNAN MEDICAL ELECTRONICS CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com