Methods for preparing human skin substitutes from human pluripotent stem cells
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INST NAT DE LA SANTE & DE LA RECHERCHE MEDICALE (INSERM)
- Publication Date
- 2011-09-14
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
technical field
[0001] The present invention relates to an in vitro method for obtaining a population of human keratinocytes derived from human pluripotent stem cells and a method for preparing a human skin substitute. Background technique
[0002] The skin is composed of self-renewing layers forming functional units of differentiated cells originating from a single basal layer of proliferating keratinocytes. Dead and dying cells that make up the stratum corneum are continuously shed during desquamation and are replaced by cells derived from epidermal stem cells in the germinal layer. Loss of epidermal function leads to loss of thermoregulation, reduced microbial defenses, risk of dryness, inhibited wound repair, and cosmetic concerns. In the absence of sufficient autologous donors for skin grafts, covering wounds with cultured human keratinocytes represents a promising therapeutic option.
[0003] Furthermore, in vitro and in vivo models of human skin can serve as excelle...