7 results about "Transferrin iron" patented technology

The invention discloses an iron-based nano-enzyme targeting chimera as well as a preparation method and application thereof. According to the chimera, lipidosome is used as a carrier, a coordination active center is constructed by Plerixabor with CXCR4 high affinity and iron ions, selective adsorption of transferrin is achieved through surface chemical design, and double-target tumor targeted enrichment is achieved by means of TfR1-mediated rapid endocytosis. And meanwhile, selective degradation of CXCR4 through a lysosome way is accelerated, so that synergistic treatment of'blocking transfer signals-chemical kinetics killing 'is realized. The preparation provided by the invention has good biocompatibility and expandability. The platform has the advantages of molecular recognition, protein crown regulation and control, microenvironment response and the like, is suitable for precise treatment of various tumors with high CXCR4 expression, and can be used as a universal membrane protein degradation and chemical kinetics treatment integrated carrier to be expanded and applied.

This utility model discloses a production device for dietary transferrin, comprising a main body of the production device, which includes a reactor one and a filter three. The main body also includes a reaction production mechanism, which includes a filter assembly one disposed on one side of the reactor one, a reaction assembly one disposed on one side of the filter assembly one, and a filter assembly two disposed on one side of the reaction assembly. By designing the reaction production mechanism, the product can undergo internal reaction and acid-base adjustment in reactors one and two during production. Utilizing the characteristic of dietary transferrin to lose iron ions in acidic conditions and bind iron ions in alkaline conditions, dietary transferrin with different iron saturations can be prepared. Simultaneously, during the filtration process of filters one, two, and three, the dietary transferrin reaction solution is recycled through a dual filtration device.

The application provides a liquid chromatography analysis method for simultaneously detecting multiple proteins, which adopts anion liquid chromatography with non-porous resin as a stationary phase, a Tris gradient eluent phase containing Cl ‑ , the non-porous resin is bonded with a hydrophilic polymer nanolayer on the surface, the nanolayer is modified with a quaternary ammonium group, the proteins have different isoelectric points, and the pH of the eluent phase is greater than the isoelectric points of the proteins. The application can simultaneously separate and purify multiple proteins through one-time anion liquid chromatography, and can approximately achieve baseline separation. The overall method is simple, economical, efficient, and environmentally friendly for the mobile phase, and is suitable for the separation of proteins with different isoelectric points, and is particularly suitable for the detection and separation of proteins such as albumin, transferrin, retinol binding protein, beta 2 microglobulin and alpha 1 microglobulin in urine.

The invention discloses a method for improving the iron binding capacity of ovotransferrin through moderate enzymolysis, and belongs to the technical field of food biology. The method comprises the following steps: (1) preparing raw materials: preparing natural ovotransferrin into a protein solution; (2) enzymolysis modification of ovotransferrin: setting the pH value of a protein solution system to a specific pH value, and performing stirring hydrolysis to prepare a hydrolyzed protein solution; and (3) enzyme inactivation: adjusting the pH value of the hydrolyzed protein solution to 7-9, and carrying out heating and water bath treatment to realize enzyme inactivation so as to obtain an enzymatic hydrolysate, namely the high-iron-binding active ovotransferrin enzymatic hydrolysate. According to the method disclosed by the invention, an ovotransferrin solution is subjected to proper enzymolysis reaction under the conditions of specific protease, specific pH, specific temperature, specific time and enzyme / substrate mass ratio, so that the ovotransferrin enzymolysis product with high iron binding capacity and oxidation resistance is prepared.

This invention relates to the field of protein extraction technology, specifically disclosing a method for the simultaneous co-production and extraction of egg white lysozyme and ovotransferrin, comprising: (1) Egg white pretreatment: after dilution, adjust the pH to 4.5, filter out the ovomucin precipitate, and then adjust the supernatant to pH 8.0-9.0. (2) Cation exchange separation of lysozyme: adsorb onto a cation exchange column of the pretreatment solution, collect the permeate and eluent as the egg white flow-through solution; elute the lysozyme, and obtain the lysozyme product by concentration, desalting, sterilization, and drying. (3) Anion exchange separation of ovotransferrin: after adjusting the flow-through solution to pH 5.5-6.0, load onto an anion exchange column, elute to remove impurities, and collect the permeate containing ovotransferrin. (4) Iron removal and drying: concentrate by ultrafiltration, dialyze with citrate buffer for desalting and preliminary iron removal, then remove bound iron by chelating resin, and dry to obtain the iron-free ovotransferrin product.

This invention relates to a multi-effect skincare essence for improving skin function and its preparation method. The invention supplements essential lipids and phospholipid precursors for the skin by adding linoleic acid and ethanolamine, strengthening the stratum corneum barrier and cell membrane structure integrity. It also adds serum albumin to address the poor water solubility and easy oxidation of linoleic acid and ethanolamine, improving the stability and skin affinity of the active ingredients. Simultaneously, it adds stable inorganic salts of essential trace elements such as selenium, silicon, tin, vanadium, manganese, and molybdenum to enhance the skin's anti-aging, repair, and firming abilities. Furthermore, it adds transferrin, which, on the one hand, blocks iron-mediated Fenton reactions and free radical generation, reducing photoaging and oxidative stress damage; on the other hand, transferrin prevents oxidative inactivation of linoleic acid, vitamins, selenium, tin, and other components under iron ion catalysis, reducing oxidative loss and protecting the formula's activity. Thus, it synergistically achieves effects such as barrier repair, anti-inflammatory soothing, anti-aging and firming, and brightening of skin tone.

The invention discloses a dynamic covalent cross-linked double protein as well as a preparation method and application thereof, and the dynamic covalent cross-linked double protein is constructed by taking lactoferrin and transferrin as raw materials and reduced glutathione as a tool to enable the lactoferrin and the transferrin to be subjected to disulfide bond exchange reaction. The method comprises the following steps: (1) respectively dissolving lactoferrin and transferrin in a phosphate buffer solution to obtain a lactoferrin solution and a transferrin solution; (2) mixing the solutions, adding reduced glutathione, and oscillating and incubating in a water bath; (3) adding EDTA to terminate the reaction; and (4) dialyzing to remove the free reduced glutathione, thereby obtaining the product. According to the lactoferrin-transferrin compound disclosed by the invention, the bacteriostatic and antioxidant functions of the two proteins are synergistically enhanced, the stability of the lactoferrin-transferrin compound in a weakly acidic environment to a weakly alkaline environment is remarkably improved, and the encapsulation efficiency of the monascus yellow liposome is improved from 55.25% to 76.25%.