30 results about "Transferrin iron" patented technology

Fusion proteins of transferrin and other therapeutic moieties with increased serum half-life or serum stability are disclosed. Preferred fusion proteins include those modified so that the transferrin moiety exhibits no or reduced glycosylation, binding to iron and / or binding to the transferrin receptor.

The invention discloses a method for preparing egg white lysozyme and active protein by adopting coseparation. The method disclosed by the invention comprises the following steps of: collecting egg white at room temperature, regulating pH to be about 5.5, stirring to be uniform and then centrifuging; adding poly vinyl alcohol with the molecular weight of 200-20000 into supernate obtained by centrifuging, stirring to be uniform and then centrifuging; adding a saturated ammonium sulphate solution into the supernate obtained by centrifuging, mixing to be uniform and then centrifuging; respectively collecting supernate and subnatant which are obtained by centrifuging; carrying out ultrafiltration or dialysis treatment on the supernate to obtain high-purity egg white lysozyme; and carrying out ultrafiltration or dialysis treatment on the subnatant to obtain other high-purity active proteins which are ovalbumin and ovotransferrin mainly. The method disclosed by the invention is simple and practicable, cost is low, conditions are mild, and good quality of lysozyme can be maintained, so that the method disclosed by the invention is applicable to large-scale industrialization production; and other active proteins can also be obtained, so that production benefit is further increased.

A molecule suitable for use as an indicator of free iron levels in a biological sample, the molecule including an iron binding moeity and a signal generating moeity, wherein an intensity of the signal generated by the signal generating moeity is related to an amount of the iron bound by the iron binding moeity.

The invention provides an iron metabolism related protein marker composition for high myopia risk prediction and high myopia auxiliary diagnosis and application of the iron metabolism related protein marker composition. Specifically, the invention provides a biomarker composition composed of serum transferrin, a ferritin light chain and a ferritin heavy chain and a kit thereof, and the biomarker composition is applied to high myopia risk prediction and high myopia auxiliary diagnosis of a to-be-detected object. In addition, the invention also provides a marker composition or a method for screening candidate compounds for preventing, delaying or treating high myopia.

Provided is a composition which can be utilized for drug delivery systems that can penetrate the blood brain barrier, and has low cytotoxicity. A nanoparticle composition comprises nanoparticles to the surface of which a first substance and a second substance each having specificity to a tumor cell are bound, wherein the first substance having specificity to the tumor cell is a peptide comprising an amino acid sequence of arginine-glycine-aspartic acid, and wherein the second substance having specificity to the tumor cell is an iron-binding protein, and wherein the nanoparticles each comprise an outer layer and vesicles enveloped by the outer layer and the nanoparticles each comprise, as membrane components, a PEGylated phospholipid, a fatty acid with a melting point of 30° C. or more, and a non-PEGylated phospholipid.

The invention provides a method for extracting protein in egg white, which mainly adopts the method of salting out precipitation combined with resin adsorption to separate ovomucin, ovotransferrin, ovalbumin, egg inhibitor, and ovomucoid from egg white at one time. and lysozyme, a total of six proteins, the method has a simple process and a short extraction period, not only avoiding the problem of toxic reagent residues, but also greatly improving production efficiency, and is easy to realize industrialization. The invention greatly improves the added value of egg processing and produces significant economic and social benefits.

The invention discloses a method for separating egg white allergens, and belongs to the technical field of protein separation. The method comprises the steps of 1) pH adjustment and centrifugation preparation of egg white treatment liquid; 2) chromatography column preparation; 3) chromatography separation; 4) protein purity detection; 5), protein concentration. The method adopts a cation exchangechromatography method, egg white is treated and then loaded, four proteins are sequentially eluted by adjusting the pH value and the salt concentration of eluent, and it is verified through protein purity detection that after a BCA method is used for detection, the extraction rates of ovalbumin, ovotransferrin, ovomucoid and lysozyme are 60.0%, 52.1%, 29.6% and 90.2% respectively.

The invention provides an ovotransferrin antibacterial peptide and a preparation method thereof. In this method, ovotransferrin is hydrolyzed by pepsin after heat treatment, separated, purified and identified to obtain three highly active antibacterial peptides. The present invention pretreats the protein by heating, which lays a good substrate foundation for pepsin hydrolysis to obtain highly active small molecule antibacterial peptides; at the same time, it adopts weak cation chromatographic column and reverse liquid chromatographic column for separation and purification, which is beneficial to obtain The peptide sequence with high antibacterial activity can be used in food industry and medical fields.

The invention discloses a method for preparing egg white lysozyme and active protein by adopting coseparation. The method disclosed by the invention comprises the following steps of: collecting egg white at room temperature, regulating pH to be about 5.5, stirring to be uniform and then centrifuging; adding poly vinyl alcohol with the molecular weight of 200-20000 into supernate obtained by centrifuging, stirring to be uniform and then centrifuging; adding a saturated ammonium sulphate solution into the supernate obtained by centrifuging, mixing to be uniform and then centrifuging; respectively collecting supernate and subnatant which are obtained by centrifuging; carrying out ultrafiltration or dialysis treatment on the supernate to obtain high-purity egg white lysozyme; and carrying out ultrafiltration or dialysis treatment on the subnatant to obtain other high-purity active proteins which are ovalbumin and ovotransferrin mainly. The method disclosed by the invention is simple and practicable, cost is low, conditions are mild, and good quality of lysozyme can be maintained, so that the method disclosed by the invention is applicable to large-scale industrialization production; and other active proteins can also be obtained, so that production benefit is further increased.

The invention belongs to the field of genetic engineering antibody technology and cell biology technology, and specifically relates to a single-chain antibody targeting transferrin receptor (TfR) in the brain. In vitro experiments have proved that the single-chain antibody targeting transferrin receptors of the present invention can specifically bind to transferrin receptors, and can be used as a small molecule carrier for diagnosis and treatment of central nervous system diseases, and can be applied to coupling brain It is of great value to the development and research of drugs for central nervous system diseases.

The invention discloses a joint separation method for lysozyme and ovotransferrin in egg white, and belongs to the technical field of protein separation and purification. The method comprises the steps that (1) the pH of egg white liquid is adjusted to 6.5-9.5, and metal-chelating cationic resin is adopted for adsorbing the lysozyme and the ovotransferrin; (2) eluant is subjected to crystallization treatment to obtain lysozyme crystals; (3) supernate after crystallization is collected, anion exchange resin is adopted for adsorption, the eluant is subjected to ultrafiltration and drying, and the ovotransferrin is obtained. The method can efficiently separate the lysozyme and the ovotransferrin from the egg white at the same time, the yield reaches 75% or above, the purity can reach 95% or above, and the adopted method has the advantages that the operation is simple, and the cost is low and is easily applied to industrialization.

The present invention provides an iron metabolism-related protein marker composition and application thereof for high myopia risk prediction and high myopia auxiliary diagnosis. Specifically, the present invention provides a biomarker composition composed of serum transferrin, ferritin light chain and ferritin heavy chain and its kit, which are applied to predict the risk of high myopia and high myopia in the subject to be tested. auxiliary diagnosis. In addition, the present invention also provides a marker composition or method for screening candidate compounds for preventing, delaying or treating high myopia.

The invention relates to the technical field of protein extraction, specifically a method for industrialized extraction of ovotransferrin and its protein iron product, which mainly uses a high-voltage pulsed electric field to assist in the treatment of egg whites to reduce viscosity and surface tension, and the molecular polarization of the electric field induces more charged electrodes Sexual groups are exposed, and the charged molecules produce repeated violent movements. This movement destroys the fibrous structure of ovomucin, disintegrates the complex formed by ovomucin and ovomucoid, lysozyme and other soluble proteins, and the thick protein is thin. The viscosity is further reduced, and the molecular dispersion plays a very good auxiliary separation effect, which is helpful for the ultrafiltration separation in the later stage and improves the protein yield; by switching to ferrous iron and ovotransferrin in egg white for chelation, not only can Enhancing the structural stability of ovotransferrin makes it possible to add high-concentration ethanol for further purification in the later stage, and it is easier for the human body to absorb ferrous iron.

The invention discloses organic metal ruthenium conjugated protein and a preparation method and application thereof. The method comprises the following steps: enabling an organic metal ruthenium antitumor drug to contact with serum protein in a buffer solution with pH value of 7-8 and concentration of 10-100mM, wherein the molar ratio of the organic metal ruthenium antitumor drug to the serum protein is (1-20):1, and the serum protein is serum albumin of which the amino acid sequence is shown by SEQ ID No:1 or transferrin of which the amino acid sequence is shown by SEQ ID No:2. Through the technical scheme, organic metal ruthenium conjugated protein with strong targeting property and relatively high antitumor activity is obtained, and experimental and theoretical basis is provided for further understanding the transport mechanism and molecular action mechanism of the organic metal ruthenium antitumor drug and preparing nano controlled release metal antitumor drug taking serum protein as a vector.