18 results about "Transferrin iron" patented technology

The invention belongs to the field of food nutrition and functional factors, and particularly relates to a method for preparing ovotransferrin hydrolysate with high immunocompetence based on an adult gastrointestinal simulated digestion system. According to the method, ovotransferrin is treated through the stomach and intestine of a simulated adult, OVT hydrolysate is prepared, and the OVT hydrolysate contains HTEGSTT, TTSY and / or RTAGWVIPMG. The invention finds that the immunomodulatory activity of OVT is enhanced after gastrointestinal digestion and hydrolysis, the OVT is more easily hydrolyzed in simulated adult digestive juice than in infant digestive juice, and a contrast experiment shows that the nutrition intervention of OVT adult and infant simulated digestion products can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid; the swelling injury caused by inflammation to small intestine mucous membrane tissues is effectively relieved, so that the intestinal barrier immune function is obviously improved, and the OIA shows higher intestinal immune activity than the OIB.

The invention provides a protein component composition with special medical application as well as a preparation method and application thereof, and relates to the field of formula food with special medical application. Wherein the protein component composition comprises protein and a sweetening agent; the protein is one or more of hydrolyzed whey protein powder, hydrolyzed casein and albumin peptide. The sweetening agent is one or more of white granulated sugar, glucose syrup, malt syrup, high fructose corn syrup, erythritol, xylitol, maltitol and stevioside. A plurality of components such as a stabilizer, mineral substances and multivitamins can also be added into the protein component composition. The finally obtained composition can be applied to preparation of formula food for special medical purposes, and the content of total protein, hemoglobin, albumin or transferrin in blood of patients suffering from malnutrition or insufficient protein intake is increased.

The present invention provides a kind of magnetic targeting, engineered extracellular vesicle loaded with hepatocyte responsive mRNA and its construction method and application, which belongs to the field of biomedicine technology. A kind of engineered extracellular vesicle, including genetically engineered extracellular vesicles, the surface of which is coupled with transferrin-modified superparamagnetic iron oxide nanoparticles and loaded with an agonist of the pro-apoptotic protein BAX; PTGFRN (Δ687) ‑ MCP fusion protein is expressed on the extracellular vesicle membrane, and MCPRNA binding protein increases the loading capacity of the extracellular vesicle to iBaxmRNA containing miR ‑ 122 recognition site; The agonist of the pro-apoptotic protein BAX is embedded in the phospholipid bilayer of the extracellular vesicle. The preparation method of the engineered extracellular vesicle of the present invention is simple, has the advantages of aortic plaque magnetic targeting, efficient nucleic acid drug loading, low side effects, etc., and provides a reference for the treatment of atherosclerosis and other age-related diseases.

Described herein are UV-resistant or UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing UV-resistant proteins such as, for example, hexokinase, heat shock proteins, alcohol dehydrogenase, transferrin, flavonol synthase, zinc oxidase, and iron oxidase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein.

The present disclosure relates to manufacturing transferrin binding proteins. Specifically, the present disclosure relates to a simple, scalable, commercially viable fermentation and purification process for obtaining recombinant transferrin binding protein (rTbp-B) along with high recovery, low impurity / aggregate content, and at the same time retains the integrity of the protein. The method uses a single chromatographic step and does not require tagging of proteins as compared to multiple chromatographic steps used previously and still manages to provide r-Tbp-B with at least 95 % purity.

The invention discloses an iron-based nano-enzyme targeting chimera as well as a preparation method and application thereof. According to the chimera, lipidosome is used as a carrier, a coordination active center is constructed by Plerixabor with CXCR4 high affinity and iron ions, selective adsorption of transferrin is achieved through surface chemical design, and double-target tumor targeted enrichment is achieved by means of TfR1-mediated rapid endocytosis. And meanwhile, selective degradation of CXCR4 through a lysosome way is accelerated, so that synergistic treatment of'blocking transfer signals-chemical kinetics killing 'is realized. The preparation provided by the invention has good biocompatibility and expandability. The platform has the advantages of molecular recognition, protein crown regulation and control, microenvironment response and the like, is suitable for precise treatment of various tumors with high CXCR4 expression, and can be used as a universal membrane protein degradation and chemical kinetics treatment integrated carrier to be expanded and applied.

The present application relates to the technical field of biological medicine manufacturing, and particularly relates to a method for extracting high-solubility ovotransferrin from egg white, wherein the egg white is diluted with water, FeCl3 solution is added, NaOH solution is used to adjust the pH of the solution, and high-speed shearing is performed; the pretreated egg white solution is subjected to ultrasonic and high-voltage alternating electric field treatment; anhydrous ethanol is added, the supernatant is obtained by centrifugation, and ultrafiltration is performed; water is added for concentration, and a crude ovotransferrin solution is obtained; resin is added to the crude ovotransferrin solution, citric acid solution is used to adjust the pH, and the resin is removed by filtration, so that a filtrate is obtained; PEG8000 is added, the pH is adjusted, and the precipitate is obtained by centrifugation; the precipitate is dissolved in water, and ultrafiltration is performed; water is continuously added for concentration, so that concentrated ovotransferrin is obtained. The extracted ovotransferrin has high solubility and iron binding capacity, can bind with iron elements again to prepare ovotransferrin iron preparation for improving iron deficiency anemia, the whole preparation process is green and environmentally friendly, has low cost, and can be mass-produced in an industrialized manner.

This utility model discloses a production device for dietary transferrin, comprising a main body of the production device, which includes a reactor one and a filter three. The main body also includes a reaction production mechanism, which includes a filter assembly one disposed on one side of the reactor one, a reaction assembly one disposed on one side of the filter assembly one, and a filter assembly two disposed on one side of the reaction assembly. By designing the reaction production mechanism, the product can undergo internal reaction and acid-base adjustment in reactors one and two during production. Utilizing the characteristic of dietary transferrin to lose iron ions in acidic conditions and bind iron ions in alkaline conditions, dietary transferrin with different iron saturations can be prepared. Simultaneously, during the filtration process of filters one, two, and three, the dietary transferrin reaction solution is recycled through a dual filtration device.

The application provides a liquid chromatography analysis method for simultaneously detecting multiple proteins, which adopts anion liquid chromatography with non-porous resin as a stationary phase, a Tris gradient eluent phase containing Cl ‑ , the non-porous resin is bonded with a hydrophilic polymer nanolayer on the surface, the nanolayer is modified with a quaternary ammonium group, the proteins have different isoelectric points, and the pH of the eluent phase is greater than the isoelectric points of the proteins. The application can simultaneously separate and purify multiple proteins through one-time anion liquid chromatography, and can approximately achieve baseline separation. The overall method is simple, economical, efficient, and environmentally friendly for the mobile phase, and is suitable for the separation of proteins with different isoelectric points, and is particularly suitable for the detection and separation of proteins such as albumin, transferrin, retinol binding protein, beta 2 microglobulin and alpha 1 microglobulin in urine.

Described herein are UV-resistant or UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing UV-resistant proteins such as, for example, hexokinase, heat shock proteins, alcohol dehydrogenase, transferrin, flavonol synthase, zinc oxidase, and iron oxidase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein.

The present disclosure relates to manufacturing transferrin binding proteins. Specifically, the present disclosure relates to a simple, scalable, commercially viable fermentation and purification process for obtaining recombinant transferrin binding protein (rTbp-B) along with high recovery, low impurity / aggregate content, and at the same time retains the integrity of the protein. The method uses a single chromatographic step and does not require tagging of proteins as compared to multiple chromatographic steps used previously and still manages to provide r-Tbp-B with at least 95 % purity.

The invention belongs to the field of bioanalytical chemistry and drug metabolism research, and discloses a typing quantitative detection method for different forms of iron in serum, which comprises the following steps: S1, mixing a to-be-detected serum sample with an inorganic chlorine salt solution, placing in an ultrafiltration centrifuge tube, standing, centrifuging, and taking filtrate as non-transferrin binding iron NTBI; s2, a weak acid solution of inorganic chlorine salt is added into the filter residues obtained in S1, centrifugation is conducted, and filtrate serves as transferrin binding iron TBI; s3, a mixed solution of a reducing agent solution and an inorganic chlorine salt solution is added into the filter residues obtained in the S2, centrifugation is conducted, and filtrate serves as drug binding iron DI; and S4, respectively measuring absorbance of the NTBI, TBI and DI parts by adopting a spectrophotometric method, and converting the concentration by combining a standard curve, so as to obtain the content of the NTBI, TBI and DI in the serum sample to be detected. The method realizes high-selectivity separation and accurate quantification of three different forms of iron, and is suitable for intravenous iron agent in-vivo behavior research and analysis of iron metabolism related mechanisms.

The invention discloses a method for improving the iron binding capacity of ovotransferrin through moderate enzymolysis, and belongs to the technical field of food biology. The method comprises the following steps: (1) preparing raw materials: preparing natural ovotransferrin into a protein solution; (2) enzymolysis modification of ovotransferrin: setting the pH value of a protein solution system to a specific pH value, and performing stirring hydrolysis to prepare a hydrolyzed protein solution; and (3) enzyme inactivation: adjusting the pH value of the hydrolyzed protein solution to 7-9, and carrying out heating and water bath treatment to realize enzyme inactivation so as to obtain an enzymatic hydrolysate, namely the high-iron-binding active ovotransferrin enzymatic hydrolysate. According to the method disclosed by the invention, an ovotransferrin solution is subjected to proper enzymolysis reaction under the conditions of specific protease, specific pH, specific temperature, specific time and enzyme / substrate mass ratio, so that the ovotransferrin enzymolysis product with high iron binding capacity and oxidation resistance is prepared.

This invention relates to the field of protein extraction technology, specifically disclosing a method for the simultaneous co-production and extraction of egg white lysozyme and ovotransferrin, comprising: (1) Egg white pretreatment: after dilution, adjust the pH to 4.5, filter out the ovomucin precipitate, and then adjust the supernatant to pH 8.0-9.0. (2) Cation exchange separation of lysozyme: adsorb onto a cation exchange column of the pretreatment solution, collect the permeate and eluent as the egg white flow-through solution; elute the lysozyme, and obtain the lysozyme product by concentration, desalting, sterilization, and drying. (3) Anion exchange separation of ovotransferrin: after adjusting the flow-through solution to pH 5.5-6.0, load onto an anion exchange column, elute to remove impurities, and collect the permeate containing ovotransferrin. (4) Iron removal and drying: concentrate by ultrafiltration, dialyze with citrate buffer for desalting and preliminary iron removal, then remove bound iron by chelating resin, and dry to obtain the iron-free ovotransferrin product.

This invention relates to a multi-effect skincare essence for improving skin function and its preparation method. The invention supplements essential lipids and phospholipid precursors for the skin by adding linoleic acid and ethanolamine, strengthening the stratum corneum barrier and cell membrane structure integrity. It also adds serum albumin to address the poor water solubility and easy oxidation of linoleic acid and ethanolamine, improving the stability and skin affinity of the active ingredients. Simultaneously, it adds stable inorganic salts of essential trace elements such as selenium, silicon, tin, vanadium, manganese, and molybdenum to enhance the skin's anti-aging, repair, and firming abilities. Furthermore, it adds transferrin, which, on the one hand, blocks iron-mediated Fenton reactions and free radical generation, reducing photoaging and oxidative stress damage; on the other hand, transferrin prevents oxidative inactivation of linoleic acid, vitamins, selenium, tin, and other components under iron ion catalysis, reducing oxidative loss and protecting the formula's activity. Thus, it synergistically achieves effects such as barrier repair, anti-inflammatory soothing, anti-aging and firming, and brightening of skin tone.

The invention discloses a portable blood iron metabolism index rapid detection micro-fluidic sensor method, and relates to the field of index detection, and the method comprises the following steps: detecting device module composition, designing a wearing housing, configuring a capacitive attachment state detection module and a determination mechanism, configuring a state indication module initialization state feedback mechanism and sampling preparation state. By constructing a portable detection structure integrating microneedle sampling, micro-fluidic guiding, iron metabolism index identification, signal conditioning and data processing and graphical interactive feedback, accurate identification and quantitative analysis of four iron metabolism core indexes including ferritin, transferrin, heme and Fe < 2 + > are realized; the collection and index judgment of the blood sample can be quickly completed on the premise that the support of large laboratory equipment is not needed; according to the method, an iron metabolism classification mechanism based on concentration ratio calculation and weight correction judgment is introduced, and the capacity of automatically recognizing storage iron deficiency, functional iron deficiency, iron load excess and other states is achieved.

The present disclosure relates to compositions and methods for treating iron overload. In particular, the methods for treating iron overload include administering to a patient an HIV protease inhibitor and an iron chelator. Iron overload may occur in patients diagnosed with anemia from inflammation or from chronic disease including hemochromatosis, sickle cell disease, thalassemia, sideroblastic anemia, an enzyme deficiency, pre-operative anemia, a cardiovascular disease, atransferrinemia, or aceruloplasminemia.

The invention discloses a dynamic covalent cross-linked double protein as well as a preparation method and application thereof, and the dynamic covalent cross-linked double protein is constructed by taking lactoferrin and transferrin as raw materials and reduced glutathione as a tool to enable the lactoferrin and the transferrin to be subjected to disulfide bond exchange reaction. The method comprises the following steps: (1) respectively dissolving lactoferrin and transferrin in a phosphate buffer solution to obtain a lactoferrin solution and a transferrin solution; (2) mixing the solutions, adding reduced glutathione, and oscillating and incubating in a water bath; (3) adding EDTA to terminate the reaction; and (4) dialyzing to remove the free reduced glutathione, thereby obtaining the product. According to the lactoferrin-transferrin compound disclosed by the invention, the bacteriostatic and antioxidant functions of the two proteins are synergistically enhanced, the stability of the lactoferrin-transferrin compound in a weakly acidic environment to a weakly alkaline environment is remarkably improved, and the encapsulation efficiency of the monascus yellow liposome is improved from 55.25% to 76.25%.