5 results about "Binding iron" patented technology

The present invention relates to a matriptase-2 (MT2) inhibitor, a TMPRSS6 inhibitor, a ferroportin blocker, or a hepcidin enhancer. In particular, the present invention relates to the use of compounds capable of reducing at least one of the following: systemic iron levels, transferrin saturation (Tsat), non-transferrin-bound iron (NTBI), and unstable plasma iron (LPI). This may occur by inhibiting the expression of a target gene, where the target gene may be a transmembrane protease, serine 6 (TMPRSS6). Furthermore, the present invention relates to compositions comprising the above compounds, and methods of using such compounds and / or compositions. Such use may include therapeutic use, for example, to reduce iron overload and prevent iron-related toxicity, before, during, or after chemotherapy pretreatment, stem cell transplantation, and engraftment.

This utility model discloses a production device for dietary transferrin, comprising a main body of the production device, which includes a reactor one and a filter three. The main body also includes a reaction production mechanism, which includes a filter assembly one disposed on one side of the reactor one, a reaction assembly one disposed on one side of the filter assembly one, and a filter assembly two disposed on one side of the reaction assembly. By designing the reaction production mechanism, the product can undergo internal reaction and acid-base adjustment in reactors one and two during production. Utilizing the characteristic of dietary transferrin to lose iron ions in acidic conditions and bind iron ions in alkaline conditions, dietary transferrin with different iron saturations can be prepared. Simultaneously, during the filtration process of filters one, two, and three, the dietary transferrin reaction solution is recycled through a dual filtration device.

This invention provides methods for quantitatively determining the concentration of free iron and ferritin-bound iron in a sample. The method includes diluting a ferritin-containing sample with a diluent to create a slurry, processing the slurry through a crossflow filtration system equipped with a membrane of at least 500 kilodaltons molecular weight cutoff, collecting the permeate containing free iron and the retentate containing ferritin-bound iron analyzing the iron concentrations in the permeate and retentate using a suitable analytical technique and calculating the mass balance of the total iron content. The invention also provides an antibody for use in quantitating the amount of ferritin in a sample and methods of treating iron deficiency in a patient in need thereof.

This invention relates to the field of protein extraction technology, specifically disclosing a method for the simultaneous co-production and extraction of egg white lysozyme and ovotransferrin, comprising: (1) Egg white pretreatment: after dilution, adjust the pH to 4.5, filter out the ovomucin precipitate, and then adjust the supernatant to pH 8.0-9.0. (2) Cation exchange separation of lysozyme: adsorb onto a cation exchange column of the pretreatment solution, collect the permeate and eluent as the egg white flow-through solution; elute the lysozyme, and obtain the lysozyme product by concentration, desalting, sterilization, and drying. (3) Anion exchange separation of ovotransferrin: after adjusting the flow-through solution to pH 5.5-6.0, load onto an anion exchange column, elute to remove impurities, and collect the permeate containing ovotransferrin. (4) Iron removal and drying: concentrate by ultrafiltration, dialyze with citrate buffer for desalting and preliminary iron removal, then remove bound iron by chelating resin, and dry to obtain the iron-free ovotransferrin product.

This invention provides methods for quantitatively determining the concentration of free iron and ferritin-bound iron in a sample. The method includes diluting a ferritin- containing sample with a diluent to create a slurry, processing the slurry through a crossflow filtration system equipped with a membrane of at least 500 kilodaltons molecular weight cutoff, collecting the permeate containing free iron and the retentate containing ferritin-bound iron analyzing the iron concentrations in the permeate and retentate using a suitable analytical technique and calculating the mass balance of the total iron content. The invention also provides an antibody for use in quantitating the amount of ferritin in a sample and methods of treating iron deficiency in a patient in need thereof.