Antihuman transferrin acceptor human source antibody and uses thereof

A human transferrin and receptor technology, applied in the field of molecular biology, can solve the problems of reduced affinity activity, inability to be applied in the human body, and difficult to completely solve the problem of antibody immunogenicity.
CN101245107BInactive Publication Date: 2010-10-13INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
Publication Date
2010-10-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an anti-human transferrin receptor human antibody and the application, which pertains to the field of molecular biology. The anti-human transferrin receptor human antibody has a single-chain antibody (scFv) amino acid sequence which is shown in a sequence table SEQ ID No.1. The anti-human transferrin receptor human antibody and the application have the advantages that: the human antibody solves the problem of immunogenicity of the target antibody, thus allowing the human antibody to carry out the long-term repeated drug administrations in the human body; the method for screening a genetic engineering antibody in a large-capacity antibody library which is adopted by the invention is easier than the monoclonal antibody preparation, the large-scale production is easy, the molecule of the antibody is small, and the antibody can enter tissues deeply, thus having obvious advantages. A pET-22b (plus) vector which is adopted by the invention contains a stronger T7 promoter, which can ensure the higher expression level of the target antibody. The induced expression conditions after the optimization can ensure that the antibody is expressed in a soluble form and theactivity of protein is higher. The 3' end of the pET-22b (plus) vector is provided with six His(histidine) labels, which can easily use the Ni-NTA agarose to carry out affinity chromatography and purification.
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Description

technical field

[0001] The present invention relates to anti-human transferrin receptor human antibody and its application, more specifically anti-human transferrin receptor human antibody, its nucleotide sequence and the carrier containing the nucleotide, belonging to molecular biology field of study. Background technique

[0002] Transferrin receptor (Transferrin Receptor, TfR) is a transmembrane glycoprotein composed of two identical subunits to form a homodimer, which mediates iron uptake through the interaction with transferrin. Transferrin receptors are expressed in nucleated cells of vertebrates, especially rich in the blood-brain barrier, placental tissue, tumor cells with vigorous metabolism, tumor cells that require a lot of iron, and some rapidly dividing cell surfaces. important target for transport.

[0003] The blood-brain barrier is composed of brain capillary endothelial cells, basement membrane and glial cells. Its remarkable feature is that the brain capi...

Claims

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