193results about How to "Molecular small" patented technology

The invention relates to an anti-human transferrin receptor human antibody and the application, which pertains to the field of molecular biology. The anti-human transferrin receptor human antibody has a single-chain antibody (scFv) amino acid sequence which is shown in a sequence table SEQ ID No.1. The anti-human transferrin receptor human antibody and the application have the advantages that: the human antibody solves the problem of immunogenicity of the target antibody, thus allowing the human antibody to carry out the long-term repeated drug administrations in the human body; the method for screening a genetic engineering antibody in a large-capacity antibody library which is adopted by the invention is easier than the monoclonal antibody preparation, the large-scale production is easy, the molecule of the antibody is small, and the antibody can enter tissues deeply, thus having obvious advantages. A pET-22b (plus) vector which is adopted by the invention contains a stronger T7 promoter, which can ensure the higher expression level of the target antibody. The induced expression conditions after the optimization can ensure that the antibody is expressed in a soluble form and the activity of protein is higher. The 3' end of the pET-22b (plus) vector is provided with six His(histidine) labels, which can easily use the Ni-NTA agarose to carry out affinity chromatography and purification.

The invention relates to a method for extracting multiple active nutrient contents from sea cucumber processing liquid. The method comprises the following steps: raw material collection, primary filtration, enzymolysis, rough filtration, ultrafiltration, concentration, secondary rough filtration, desalination, fermentation, centrifugation, secondary enzymolysis, secondary centrifugation, third rough filtration, secondary ultrafiltration, secondary concentration and spray drying. The invention further relates to application of the method in orally disintegrating tablets. Compared with the prior art, the method has the advantages that a prepared sea cucumber extract contains multiple nutrient contents, and the total content of sea cucumber polypeptide, small proteins, sea cucumber mucopolysaccharide and sea cucumber glycosides reaches 80%-90%; in the whole technological process, reaction conditions are mild, no organic solvent is used, nutrient contents are hardly damaged, and molecules of the extracted sea cucumber extract are relatively small; after the sea cucumber extract is fermented by using beneficial bacteria, the nutrient contents in the sea cucumber extract are easily absorbed by human bodies, and almost no peculiar smell exists; furthermore, the technological process is very suitable for the industrial expanded production.

The invention discloses a method of preparing high F-value collagen peptide by hydrolyzing anglerfish fishskins. The method comprises the following steps: by taking anglerfish fishskins as a raw material, extracting collagen by acid liquor; then, fractionally hydrolyzing fishskin collagen by using stomach protease and flavourzyme under an ultrasonic condition; and finally, removing aryl and color, and after vacuum concentration, carrying out spray drying to obtain the high F-value collagen peptide. According to the method disclosed by the invention, leftovers of the anglerfish fishskins are effectively utilized, and the high F-value collagen peptide is prepared by hydrolyzing the anglerfish fishskins, so that not only is high nutritional value of collagen peptide extracted from the anglerfish fishskins utilized, but also the problem that the leftovers of the anglerfish fishskins pollute the environment and lead to waste of resources is further solved. The collagen peptide prepared by the method disclosed by the invention has high F value and small relative molecular weight and is beneficial to be absorbed by the human body. The value of the collagen peptide is higher than that of common collagen peptide.

The invention discloses a low-temperature ultrahigh-pressure continuous sterilization method for liquid milk, relates to liquid milk, and particularly relates to a method for sterilizing the liquid milk by adopting ultrahigh-pressure water jet. The method is characterized in that the liquid milk is subjected to the ultrahigh-pressure water jet treatment in a nozzle of coaxial equivalent clash type or collision type at the temperature of between 4 and 60 DEG C under the pressure of 50 to 400MPa; and the liquid milk overcomes the defects that the pasteurized milk has short shelf life and the instantaneous ultrahigh-temperature sterilized milk has large loss of nutrient components, and can preserve the nutrition, prolong the shelf life, save time and labor and reduce the energy consumption. The method is applied to sterilization of cow milk, yak milk, water buffalo milk and goat milk, and the sterilized milk is expected to become a new variety of the liquid milk, namely ultrahigh-pressure milk.

The invention relates to manufacture of a hollow capsule for food and medicine, in particular to a raw material composition for preparing a starch matrix hollow capsule, and the starch matrix hollow capsule prepared from the raw material composition and easy for rapid disintegration. The raw material composition for preparing starch matrix hollow capsule of the invention is the prepared by mixing hydroxypropyl starch, hydroxypropyl-beta-cyclodextrin and a gelling agent. The raw material composition is mixed with water, heated, gelatinized and gelatinized, so as to prepare the starch matrix hollow capsule. The invention introduces a proper amount of hydroxypropyl-beta-cyclodextrin with excellent water solubility into the raw material composition, so that the prepared starch matrix hollow capsule has good disintegration performance and does not affect the capsule strength.

The invention provides a single-domain antibody fusion protein. The single-domain antibody fusion protein comprises one or more single-domain antibodies fused with cytokines, in particular one or moreanti-PD-1 single-domain antibodies fused with IL-12 or IL-2. The invention also provides an application of the single-domain antibody fusion protein in preparing medicines for treating tumors. The fusion protein provided by the invention actively combines the advantages of small molecules, good permeability, high stability and the like of single-domain antibodies with the immunoregulation and thelike of the cytokines, and has a better anti-tumor effect.

The invention discloses Kushui rose oil soft capsules, which are prepared by mixing pure natural vegetable essential oil. The raw material in the soft capsules comprises the following components in percentage by weight: 4 to 10 percent of Kushui rose essential oil, 12 to 18 percent of sea buckthorn berry oil, 18 to 25 percent of grape seed oil, 12 to 20 percent of perilla herb oil, 10 to 15 percent of sweet almond oil, 13 to 18 percent of safflower oil, 8 to 13 percent of rosemary oil and 4 to 8 percent of peppermint oil. The soft capsules are prepared by mixing the pure natural vegetal essential oil including the Kushui rose essential oil, sea buckthorn berry oil, grape seed oil, perilla herb oil, sweet almond oil, safflower oil, rosemary oil and peppermint oil. The Kushui rose oil soft capsules have the effects of soothing liver for relieving depression, activating blood, stimulating the menstrual flow, relieving cholecystitis, hepatalgia and qi and stomach disharmony, nourish skin, whitening and resisting wrinkles. The Kushui rose oil soft capsules have the advantages of delicately combining several kinds of pure natural vegetable essential oil, along with greenness, naturalness, organic property and no toxic and side effect.

The invention discloses a fireproof foam board and a production method thereof. The fireproof foam board comprises a waterborne binding agent solution, polystyrene particles, magnesia, talcum powder, wood powder and a foaming agent in volume ratio of 0.3:1:0.2:0.05:0.1:0.0005, wherein the waterborne binding agent solution is prepared from water and a waterborne bonding agent according to the volume ratio of 1:450; weighing various materials according to the formula; feeding the wood powder in the waterborne binding agent solution firstly; uniformly stirring and pouring the magnesia and the talcum powder; uniformly stirring and feeding the foaming agent for foaming; after foaming, enabling the mixture and the polystyrene particles to simultaneously enter the interior of a special stirrer and uniformly stirring; after pouring the stirred mixture in a die and applying pressure of 400kilos, pushing the mixture in a greenhouse with 40DEG C and drying for one hour; and finally demoulding and cutting into boards with a certain specification by using a dicing saw. The fireproof foam board using polystyrene foam has the characteristics of excellent heat-insulating performance, reasonable formula, reached A-level fireproof property, environment protection, no pollution, and simple producing and mounting method.

The invention relates to balanced nutrition multi-grain rice with easy digestion and absorption and a preparation method thereof. The preparation method comprises the steps of: performing extrusion treatment on a coarse food grain mixture, wherein the coarse food grain mixture comprises one or more of potatoes, yams, pumpkins, taroes, soybeans, chestnuts, horsebeans, peas, barley and the seed of Job's tears; and fully mixing the coarse food grain mixture after the extrusion treatment with rice, and packaging the mixture, wherein the coarse food grain mixture accounts for over 1 percent of the total weight of the coarse food grain mixture and the rice, namely is more than or equal to 1 percent. The balanced nutrition multi-grain rice with easy digestion and absorption does not need to be presoaked for certain time before the boiling, has the advantages that the rice can be quickly boiled, is easy to be digested and absorbed by human body, can achieve the effect of nutrition complementation of cereals, yams and beans, has balanced nutrition, gives consideration to the mouthfeel and the taste, and can simply, conveniently, scientifically and synchronously supplement various dietary fibers, vitamins, unsaturated fatty acids and proteins.

The invention relates to the technical field of cosmetics and discloses a multifunctional skin essence which comprises the following components in percentage by mass: 78.38% of water, 5% of glycerinum, 3% of polysorbate-80, 3% of polyethylene glycol-400, 2% of glyceryl stearate, 2% of polyethylene glycol-60, 1% of ethanol, 0.5% of a cladosiphon okamuranus extract, 0.5% of a ginseng root extract, 0.2% of glycine betaine, 0.03% of hyaluronic acid, 0.2% of a vaccinium myrtillus extract, 0.2% of a hydroxyethyl cellulose, 0.5% of butanediol, 0.2% of dipotassium glycyrrhizinate, 0.4% of carbomer, 0.5% of natto gum, 0.05% of tranexamic acid, 0.2% of potassium 4-methoxysalicylate, 0.2% of a yeast extract, 0.15% of methylparaben, 0.1% of potassium hydroxide, 0.02% of ascorbic acid magnesium phosphate, 0.02% of EDTA (Ethylene Diamine Tetraacetic Acid) disodium, 0.1% of a hamamelis virginiana extract, 0.02% of sodium polyglutamate, 0.5% of a glycyrrhiza glabra root extract, 0.5% of a malva sylvestris flower extract, 0.3% of a chamomilla recutita flower extract, 0.2% of a peony root extract and 0.03% of an essence. The essence plays roles of a toner, a lotion and an essence, three effects arecombined into one, one bottle of essence can replace three bottles of cosmetics, and skin nursing procedures can be simplified.

The invention discloses an activated carbon material and a preparation method thereof and a supercapacitor, which belong to the technical field of electrode materials. The activated carbon material is prepared by taking blocky graphite as a raw material through heating the blocky graphite to 500-700 DEG C at a heating rate of 10-20 DEG C/min; carbonizing the blocky graphite, and heating the blocky graphite to 700-900 DEG C at a heating rate of 0-20 DEG C/min; and feeding activation gas for activating the obtained object, so that an activated carbon material with large specific surface area, large total pore volume and high porosity is obtained. Because the blocky graphite is taken as a raw material, the activated carbon material is wide in source and low in price, thereby saving cost. The preparation method disclosed by the invention is mild in activation conditions, small in energy consumption, environmentally friendly, easy to operate, wide in raw material sources, low in cost, and suitable for large-scale industrial production. The supercapacitor disclosed by the invention takes the activated carbon material prepared according to the invention as an electrode material, so that the specific capacitance and charge-discharge cycle performance of the supercapacitor are improved, and the requirements of the supercapacitor on high specific capacitance and excellent large current properties are met.

The invention relates to the technical field of fresh-keeping, in particular to a fresh-keeping agent and fresh-keeping method for grosvenor momordica fruits. The fresh-keeping agent consists of fresh-keeping components and a film-forming agent. The fresh-keeping components consist of grosvenor momordica fruit seed essential oil, clove essential oil, herba pogostemonis essential oil, licorice extractive, grape seed essential oil and lotus leaf extractive. The fresh-keeping components of the fresh-keeping agent employ four essential oil components as main fresh-keeping active ingredients. The essential oil has the characteristics of small molecules, good permeability, high content of active ingredients and easy decomposition, and the fresh-keeping agent is an effective fresh-keeping agent,and has good antibacterial, bactericidal and anti-oxidation effects. The film-forming agent is mainly composed of the following components including burdock oligosaccharide, chitosan, sodium alginateand konjac gum, and the components have a bacteriostatic effect besides a film-forming characteristic, can slow down the respiration of the grosvenor momordica fruits and form a protective film on thesurface of each grosvenor momordica fruit, and enable mogroside V and total mogroside to be effectively gathered in the grosvenor momordica fruits.

The invention relates to a medical preparation containing organic effective constituents, in particular to the application of a compound 3',4-dyhydroxyl-3,5'-dimethoxybibenzyl in preparing medicaments for treating cataract. The compound has double-target inhibition activity, and not only can inhibit the activity of AR to resist high osmotic pressure, but also can inhibit the activity of iNOS to resist oxidation, thus the compound has good effect on resisting the cataract. The compound has small molecules, thereby not only being easy to absorb by the organism, but also being capable of penetrating cell membranes without accumulation effect and toxic and side effects.

The invention discloses a blood-glucose-reducing polypeptide herbal solid drink, and a preparation method of liver peptide powder. The blood-glucose-reducing polypeptide herbal solid drink comprises the following ingredients in parts by weight: 2.0-3.0 bone marrow peptide powder, 0.2-0.5 part of liver peptide powder, 2.8-3.2 parts of a kelp extract, 2.3-2.6 parts of a Chinese yam extract, 1.85-2.10 parts of a kudzuvine root extract, 1.3-1.8 parts of a radix polygonati officinalis extract, 0.7-1.0 part of a Chinese angelica root extract, and 0.4-0.6 part of a cinnamon extract. The preparation method of the liver peptide powder comprises the following steps: (1) carrying out pretreatment; (2) carrying out sterilization; (3) carrying out enzymatic hydrolysis; (4) carrying out inactivation andsterilization; (5) carrying out filtration and separation; (6) carrying out concentration; and (7) carrying out drying so as to obtain a final product. The bone marrow peptide powder and the liver peptide powder are combined with the herbs used as both medicines and foods so that the blood-glucose-reducing polypeptide herbal solid drink has more significant effects, and is free of side effects and high in safety.

The invention relates to a health-care food having functions of balancing nutritive cells and recuperating five internal organs. A making method of the health-care food comprises the following steps of taking cereal type materials and seed type materials, performing stir-frying, and performing grinding to obtain 200-mesh powder; and performing freeze-baking on other materials, performing grindingto obtain 200-mesh powder, and performing mixing to obtain the health-care food. The health-care food is purely natural, free from additives, free from transgenesis, free from pesticides and heavy metal remains, scientific and professional in formula, comprehensive in nutrient collocation, convenient to eat, and good in mouth feel, is suitable for different crowds to eat for a long term, is free from any side effects, and can improve body healing capacity, delay senescence and prolong life.

The invention discloses a preparation method of a polypeptide soybean protein complex fiber, which comprises the following steps that (1), soybean protein is soaked in a diluted alkali solution, and stirred at a high speed, then a soybean protein solution is obtained, and subjected to ultrafiltration dialysis by an ultrafiltration membrane, and a polypeptide soybean protein mother solution with protein molecular weight of 300-2000 is prepared; (2), the polypeptide soybean protein mother solution is added to a polyvinyl alcohol solution, a cross-linking agent accounting for 1.0-2.0wt% of polyvinyl alcohol is then added, and mixed uniformly by high-speed stirring, and a polypeptide soybean protein and polyvinyl alcohol blended solution is prepared; and (3), the polypeptide soybean protein and polyvinyl alcohol blended solution is filtered, defoamed, and then subjected to spinning forming, strand drafting and aftertreatment to form the polypeptide soybean protein complex fiber. According to the preparation method, production steps are simplified; the manufacturing cost is low; no aldehyde pollution exists; industrial production is facilitated; the protein molecular weight is low; the loss amount of the protein is low; and maintaining of excellent performance of the protein fiber is facilitated.

The invention discloses skin rejuvenating and regeneration essence. The skin rejuvenating and regeneration essence comprises collagen, fibroin, coriolus versicolor extract, ceramide, hyaluronic acid, vitamin E acetate and water. The skin rejuvenating and regeneration essence has the advantages of small molecules, natural and mild properties and no irritation, can be used for moisturizing skin for a long time, has good water replenishment effects on dry skin and sensitive skin, can improve skin elasticity, realizes combination of regeneration, water replenishment, spot fading and regeneration, improves skin moisturizing and regeneration effects, and soothes the traces of the years. The powerful essence can be fast absorbed by skin, make skin elastic and compact and provide silk-like gloss for the skin.