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Polypeptide drug exosome nano drug-loading system targeting cell membrane and preparation method of system

A nano-drug loading and exosome technology, which is applied in the field of genetic engineering and nano-materials, can solve the problems of inability to improve the treatment effect of tumors, and achieve the effect of improving the treatment effect, improving stability and improving the treatment effect

Active Publication Date: 2020-03-31
饶磊
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But usually, the receptors of various cytokines are distributed on the cell surface, and most of the cytokines are delivered into the cells, which cannot improve the therapeutic effect of tumors

Method used

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  • Polypeptide drug exosome nano drug-loading system targeting cell membrane and preparation method of system
  • Polypeptide drug exosome nano drug-loading system targeting cell membrane and preparation method of system
  • Polypeptide drug exosome nano drug-loading system targeting cell membrane and preparation method of system

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Experimental program
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Embodiment 1

[0048] The preparation of embodiment 1 drug-carrying system

[0049] Firstly, exosomes are used to load polypeptide drugs, and genetic engineering is used to complete the loading of exosomes on polypeptide drugs, and then the peptide-loaded exosomes are combined with transferrin-modified superparamagnetic nano-iron. The specific steps are as follows:

[0050] (1) Construction of recombinant plasmids

[0051] Using overlapping PCR technology, the tumor necrosis factor TNF-α extracellular segment sequence (extracted by reverse transcription PCR) was connected and fused with the flexible heptapeptide and the penetrating peptide CPP sequence to construct the fusion protein gene; the connection was carried out by designing two pairs of primers The reverse transcribed cDNA was amplified with primers F1 and R1 for the TNF-α extracellular segment sequence, and the CPP sequence was amplified with primers F2 and R2. By overlapping PCR technology, the TNF-α extracellular segment gene was...

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Abstract

The invention provides a polypeptide drug exosome nano drug-loading system targeting a cell membrane and a preparation method of the system, and constructs the nano-delivery system (CTNF-alpha-exosome-SPION) having active targeting performance. Fusion polypeptides obtained through genetic engineering are distributed to the surfaces of exosomes, and SPION is connected to the exosomes through the combination of transferrin and transferrin receptors on the exosomes; with the help of an external magnetic field, the active targeting of the SPION allows a load drug to achieve a higher local concentration; and in addition, through lipophilicity of the fusion protein and delivery of the exosomes, more fusion protein is combined with receptors distributed on the cell surfaces to enhance activationactivity. The drug-loading system can deliver the polypeptide drug to the location of the polypeptide receptors on the cell surfaces of a lesion site, thereby improving the targeting and activation activity of the polypeptide drug.

Description

technical field [0001] The invention belongs to the fields of nanomaterials and genetic engineering, and in particular relates to an exosome nanometer drug-carrying system capable of targeting cell membranes and a preparation method thereof. Background technique [0002] In recent years, advances in surgery and multiagent chemotherapy have improved surgical outcomes and quality of life for patients. However, most clinical anticancer drugs have significant side effects due to their toxicity to normal cells and lack of targeting. The emergence of nanobiomaterials has opened up new prospects for cancer drug therapy. Synergistic therapy utilizing nanomaterial systems can significantly reduce the dose of anticancer drugs, alleviate toxic side effects, and increase targeted drug delivery. [0003] Tumor necrosis factor is the earliest cytokine used in cancer therapy, and tumor necrosis factor significantly inhibits tumor angiogenesis and cancer cell proliferation. However, natu...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K41/00A61K38/19A61K47/64A61K47/46A61K47/02A61K47/42A61P35/00C12N15/62C12N15/85C12N5/10
CPCA61K9/0009A61K9/5115A61K9/5169A61K9/5176A61K38/00A61K41/00A61P35/00C07K14/525C07K2319/00C07K2319/10C12N15/85
Inventor 饶磊庄满娇
Owner 饶磊
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