114 results about "Cell selectivity" patented technology

The present invention is based on the discovery that proteins produced in insect cell cultures are glycosylated in a unique manner that causes them to be selectively imported by cells that express mannose receptors on their membranes, particularly macrophages. Proteins that are selectively imported into cells containing mannose receptors are provided, as well as pharmaceutical compositions containing such proteins and methods for producing such proteins. Application of the present invention to produce proteins useful for treating lysosomal storage disorders is also disclosed. Engineering of cells to express mannose receptors so that they will selectively import proteins produced in insect cells is also taught, as well as a protein targeting system using such cells and proteins. Finally, an improved elution buffer for the purification of proteins produced in insect cells from a Concanavalin A column is provided.

Disclosed are compositions and methods useful for targeting and internalizing molecules into cells of interest and for penetration by molecules of tissues of interest. The compositions and methods are based on peptide sequences that are selectively internalized by a cell, penetrate tissue, or both. The disclosed internalization and tissue penetration is useful for delivering therapeutic and detectable agents to cells and tissues of interest.

Disclosed is a device for sticking a plurality of cells to one basement in sequential arrays, which is characterized in that the upper surface of the basement is sequentially plated with a titanium adhesion layer, a golden layer, bands of mercaptan hydrophobic monolayer and bands of mercaptan hydrophilic monolayer, and the bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer are parallel attached on the golden layer at intervals; the lower surface is provided with a second polydimethylsiloxane seal of a micro-groove unit; the micro-groove unit is composed of three parallel linear grooves; the ends of the grooves are provided with vertical through holes communicated with the grooves; the lower surface of the seal is coated on the surface of the basement with parallel bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer; the linear grooves are perpendicular to the bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer; and the golden surface of the basement is formed into a zone for the cells to be stuck on selectively. Substances (such as protein solution of the extra cellular matrix and polylysine solution, etc.), which facilitate the cells to be stuck, are injected into microflow pipes; after the cells are stuck selectively, different cell suspensions are injected into different pipes, and the cells are only stuck on the surfaces of the hydrophobic monolayer; then the seal is taken off, a plurality of different cell arrays are sequentially stuck on the basement.

The present invention relates to an implant (1) with at least partially a roughened surface (2), which is at least partially covered by an organic or polymeric intermediate layer (3) and attached thereto a top layer (4). The top layer (4) provides for a controlled release of at least one bioactive agent. A kit for preparing such an implant is also described.

The invention provides a heterozygosis alpha spiral swine antibacterial peptide, and a preparation method and application thereof. The heterozygosis alpha spiral swine antibacterial peptide has the sequence shown as SEQ No.1 in a sequence table. According to the method, firstly, 16 amino acid residues before N- tail end in the swine antibacterial peptide PMAP-36 are modified, and are then subjected to heterozygosis with the front 15 amino acid of Fowlicidin-2 to obtain the antibacterial peptide PR-FO; the tail end of the carboxyl of the PR-FO is amidated so as to increase the positive charge and to improve the peptide stability; the sequence is shown as SEQ No. 1; then, a polypeptide synthesis instrument is used for synthesizing the antibacterial peptide PR-FO by a solid-phase synthesis method. The antibacterial peptide provided by the invention has higher antibacterial activity and low cell toxicity; the cell selectivity is greatly improved. The method can lay the feasible theoretical and practice foundation for making the antibacterial peptide into antibiotic substitutes.

The present invention provides methods and compositions for conferring tumor selective cell death on cancer cells expressing specific target precursor messenger RNA molecules (cancer cell selective target pre-mRNAs). The compositions of the invention include conditionally replicative adenoviruses that have been genetically engineered to express one or more pre-trans-splicing molecules (PTMs) designed to interact with one or more cancer cell target pre-mRNA and mediate a trans-splicing reaction resulting in the generation of novel chimeric RNA molecules (chimeric RNA) capable of encoding adenovirus specific protein(s). Adenovirus specific proteins include those proteins complementing an essential activity necessary for replication of a defective adenovirus. The methods and compositions of the invention may be used to target a lytic adenovirus infection to cancer cells thereby providing a method for selective destruction of cancer cells. In addition, the adenoviruses of the invention may be engineered to encode PTMs designed to interact with target pre-mRNAs encoded by infectious agents within a cell, thereby targeting selective destruction of cells infected with such agents.

The invention belongs to the field of the membrane penetrating peptide and particularly relates to a tumor cell membrane selectively penetrating peptide and application thereof. The sequence of the tumor cell membrane selectively penetrating peptide comprises a basic amino acid cluster which is composed of 5 to 12 consecutive arginine residues. The tumor cell membrane selectively penetrating peptide is characterized in that the C or N end of the basic amino acid cluster is connected with three histidine residues. The tumor cell membrane selectively penetrating peptide has cell membrane penetration capacity, can distinguish tumor cells and normal tissue cells and can efficiently penetrate through the tumor cell membrane without penetrating through the normal tissue cell membrane basically.

The present invention relates to a microfluidic device used for cell motility screening and chemotaxis testing which comprises microfluidic channels and chambers. Cells which can secret a chemoattractant or chemorepellent are selectively planted in the microfluidic device and a stable chemoattractant or chemorepellent gradient can be established in the channels.

The invention provides a multi-stranded beta-hairpin short-peptide with tolerant protease and a preparation method and an application. A sequence is as shown in a SEQ ID No.1. The short-peptide provided by the invention fully exerts the structural advantages of a beta-hairpin structure to obtain a multi-stranded beta-hairpin structural antibacterial peptide. The stability is further improved while the activity is ensured by using a common amino, and composition of a corner amino acid is changed, so that a series of multi-stranded beta-hairpin antibacterial peptides of brand new structures: (WRXxRW)n-NH2, wherein n is 1, 2, 3 and 4; PG is selected as a corner to describe; when n is equal to 2, the antibacterial peptide is named W2; and when n is equal to 3, the antibacterial peptide is named W3. The peptide verifies relatively high cell selectivity and salt ion tolerance, and moreover, based on the activity of the antibacterial peptide with structural stability, the obtained antibacterial peptide is also relatively short, and relatively strong tolerance of in vivo protease is also represented.

A skin care composition for stimulating selective catabolysis in cells of keratin surfaces comprising at least one autophagy activator and at least one proteasome activator, and a method for improving selective catabolysis in cells of keratin surfaces by treating with the composition.

The invention provides a tryptophan tension chain-beta-hairpin antibacterial peptide and a preparation method and application thereof. A sequence of the antibacterial peptide is as shown in a sequence table SEQ ID No.1. The preparation method comprises the following steps: by taking a tryptophan tension chain structure as a template, combining amino acid composition and structural characteristics of the antibacterial peptide; and by using interaction of cross-chain W-W as a structural stable factor, symmetrically and circularly arranging an amino acid lysine with charges and a hydrophobic amino acid tryptophan as repeated binary sequence units on two arms of the beta-hairpin to obtain the antibacterial peptide WK3. The antibacterial peptide has relatively high bacteriostatic activity and cell selectivity, and the treatment index reaches up to 161.27. The antibacterial peptide designed by the method needs not to bind a disulfide bond but has extremely high structural stability, so that the selectivity of the antibacterial peptide between bacterial cells and mammalian cells is improved, and the antibacterial peptide has an application potential as an antibiotic substituent. The antibacterial peptide is relatively high in activity and relatively low in cytotoxicity.

The fixing process of biological macromolecule on inorganic silicon material surface in common pattern includes the following steps: amination and aldehydration treatment of the inorganic silicone material surface; polydimethyl siloxane stamp micro contact printing on the surface to form one biological macromolecule miro pattern; dropping one other biological macromolecule solution to fix in the uncovered area; and washing and ultrasonic treatment to form the co-pattern fixation of two kinds of biological macromolecules. The said process is simple, controllable and repeatable and has imld reaction condition. The present invention has excellent application foreground in selective cell adhesion, direction induction, cell-based biological device making, tissue engineering, etc.

The invention discloses a targeted protein degradation c-Met degradation agent as well as a preparation method and application thereof. The invention provides a c-Met degradation agent based on a targeted protein degradation PROTAC strategy, a preparation method thereof and application of the c-Met degradation agent in the treatment of non-small cell lung cancer, gastric cancer and other cancers.The compound has a remarkable c-Met degradation effect and a remarkable cell proliferation inhibition effect, has the potential of serving as an anti-tumor drug to treat tumors, shows remarkable proliferation inhibition activity in an EBC1 drug-resistant cell strain constructed by lentiviral transfection, is obviously superior to a small molecule inhibitor LXM262. and obviously improves the cell selectivity. The compound has significant advantages in overcoming tumor c-Met acquired drug resistance, especially the compound S27 only provides good activity for c-Met dependent EBC-1 lung cancer cells, it is indicated that the compound S27 has good cell selectivity while the original small molecule inhibitor is multi-target, and the compound also has an inhibition effect on a c-Met non-dependent cell line.

Angiogenic endothelial cells are selectively targeted with lipid/DNA complexes or cationic liposomes containing a substance which affects the targeted cells by inhibiting or promoting their growth. A site of angiogenesis can be precisely located by administering cationic liposomes containing a detectable label. The complexes may comprise nucleotide constructs which are comprised of promoters which are selectively and exclusively activated in the environment of an angiogenic endothelial cell.

The invention provides a novel brain targeting preparation for preventing and treating a neurodegenerative disease. The novel brain targeting preparation comprises effective dose of fusion protein of cell growth factor and TAT (transcriptional activator) cell-penetrating peptide, or fusion protein of the cell growth factor, TAT and a transferrin receptor monoclonal antibody, a protein activity protecting agent, and proper auxiliary material, wherein the fusion protein of cell growth factor and TAT cell-penetrating peptide or the fusion protein of the cell growth factor, TAT and transferrin receptor monoclonal antibody is an active ingredient. The novel brain targeting preparation disclosed by the invention has excellent performance of breaking through a blood brain barrier, and is specifically targeted to the brain; the defect that single TAT does not have cell selectivity can be overcome; a brain neurons microenvironment is regulated and controlled by the effects of neurotrophy, paracrine and the like, so as to play the roles of preventing and treating the neurodegenerative disease. The novel brain targeting preparation can be prepared into a freeze-drying preparation, a liquid preparation or an adhesive preparation and applied in an intravenous injection or nasal delivery manner.

The invention discloses a method and a system for selecting an individualized tumor neoantigen based on an expected curative effect. The method comprises the following steps: establishing and optimizing a cell selectivity model; establishing and optimizing a treatment effect model, carrying out auxiliary selection on individualized tumor neoantigens and the like, and can maximize the utilization of biological experiments to carry out trial and error, establish the most accurate cell selectivity model and maximize the utilization of the biological experiments to improve the effect on individual patients under the condition of not involving the patients; the method further considers the correlation between the selectivity of new antigen cells and the immune state of patients, builds a complete curative effect prediction model, evaluates the applicability of a specific new antigen to a specific patient according to an actual curative effect, provides high-value reference information for a doctor to select an appropriate new antigen for immunotherapy, utilizes the actual effect feedback of doctor treatment to the maximum extent, and continuously improves the prediction precision of the overall effectiveness of new antigen immunotherapy.

Disclosed are compositions and methods useful for targeting and internalizing molecules into cells of interest and for penetration by molecules of tissues of interest. The compositions and methods are based on peptide sequences, such as truncated LyP-1 peptides, that are selectively internalized by a cell, penetrate tissue, or both. The disclosed internalization and tissue penetration is useful for delivering therapeutic and detectable agents to cells and tissues of interest.