Device for adhering various cells on same substrate orderly and adhering method
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A substrate and cell technology, which is applied in the field of devices and adhesions that adhere multiple cells to the same substrate in an orderly array, and can solve problems such as difficult construction of cell arrays, inability to interact, complex chemical synthesis, etc.
Inactive Publication Date: 2008-12-31
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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[0006] The purpose of the present invention is to overcome the difficulty in constructing cell arrays when a variety of cells are attached to the same substrate surface in the prior art, so that the fixed cell pattern is des
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Embodiment 1
[0040] Please refer to Figure 2-1, Figure 2-2, Figure 2-3, Figure 2-4, Figure 2-5 and Figure 2-6:
[0041] 1) Evaporating a 2-10 nm thick titanium adhesion layer on the upper surface of a clean glass substrate 1, and then evaporating a 20-50 nm thick gold layer thereon;
[0042] 2) Using photolithography technology, prepare an array structure with 50 parallel convex strips with a width of 100 μm (20-300 μm is acceptable), a length of 2 cm, and an interval of 200 μm on a silicon wafer;
[0043] 3) Flip the silicon wafer having an array structure of 50 parallel convex strips obtained in step 2) with polydimethylsiloxane, and obtain a silicon wafer corresponding to the array structure of the parallel convex strips. The first polydimethylsiloxane stamp with an array structure of 49 parallel convex strips;
[0044] Put the resulting stamp into 10 mM methyl terminated thiol (molecular formula: HS(CH 2 ) 11 CH 3 ) in the ethanol solution; then take it out, dry it with nitrogen or...
Embodiment 2
[0053] 1) First evaporate a 5nm (2-10nm) thick titanium adhesion layer on the upper surface of a clean glass slide (thickness 0.15mm), and then evaporate a 40nm (20-50nm) thick titanium adhesion layer on it ) a thick gold layer to make a test substrate 1;
[0054] 2) Using photolithography technology, prepare an array structure with 100 parallel convex strips with a width of 100 μm (20-300 μm is acceptable), a length of 2 cm, and an interval of 100 μm (20-300 μm is acceptable) on a silicon wafer ;
[0055] 3) Flip the silicon wafer with an array structure of 100 parallel convex strips obtained in step 2) with polydimethylsiloxane, to obtain a film corresponding to the array structure of the parallel convex strips. The first polydimethylsiloxane stamp with an array structure of 99 parallel convex strips;
[0056] Put the resulting stamp into 10 mM methyl terminated thiol (molecular formula: HS(CH 2 ) 15 CH 3 or HS(CH2) 17 CH 3 ) in the ethanol solution; then take it out,...
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Abstract
Disclosed is a device for sticking a plurality of cells to one basement in sequential arrays, which is characterized in that the upper surface of the basement is sequentially plated with a titanium adhesion layer, a golden layer, bands of mercaptan hydrophobic monolayer and bands of mercaptan hydrophilic monolayer, and the bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer are parallel attached on the golden layer at intervals; the lower surface is provided with a second polydimethylsiloxane seal of a micro-groove unit; the micro-groove unit is composed of three parallel linear grooves; the ends of the grooves are provided with vertical through holes communicated with the grooves; the lower surface of the seal is coated on the surface of the basement with parallel bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer; the linear grooves are perpendicular to the bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer; and the golden surface of the basement is formed into a zone for the cells to be stuck on selectively. Substances (such as protein solution of the extra cellular matrix and polylysine solution, etc.), which facilitate the cells to be stuck, are injected into microflow pipes; after the cells are stuck selectively, different cell suspensions are injected into different pipes, and the cells are only stuck on the surfaces of the hydrophobic monolayer; then the seal is taken off, a plurality of different cell arrays are sequentially stuck on the basement.
Description
technical field [0001] The invention relates to a device and an adhesion method for adhering various cells to the same substrate, in particular to a device and an adhering method for adhering various cells to the same substrate in an orderly array. Background technique [0002] In the research of drug screening, biological detection chip and basic cell biology, it is necessary to fix a variety of cells in an array on the same surface, and more precise and complex control of cell culture in vitro is also of great significance for the construction of artificial organs . Despite the difficulty, people have done some methods. [0003] For example in literature 1: Chiu, D.T.; Jeon, N.L.; Huang, S.; Kane, R.S.; Wargo, C.J.; , (6), 2408-2413, the Whitesides experimental group disclosed a method of immobilizing various cells on the same surface using a three-dimensional microfluidic channel, and can form a complex pattern composed of various cells. This method first prepares a th...
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IPC IPC(8): C12N11/00
Inventor 李勇谢赟燕陈振玲邢仕歌袁博蒋兴宇
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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