The invention discloses a hydrogel modified
microelectrode array device and a preparation method thereof, the hydrogel modified
microelectrode array device comprises a
microelectrode array composite layer, a
passivation layer, a hydrogel layer and a
cell culture ring, the
cell culture ring is arranged on the microelectrode array composite layer, and the
passivation layer and the hydrogel layer are located in the
cell culture ring; the microelectrode array composite layer comprises a microelectrode array with
polydimethylsiloxane as a base material, at least part of the microelectrode array in the
cell culture ring is exposed, and at least part of gaps of the exposed microelectrode array are filled with the
passivation layer; the hydrogel layer grows on the surface of the polydimethylsilane, and a microgroove is formed in the exposed microelectrode array and passivation layer area to serve as a
cell growth area. According to the invention,
cell patterning can be realized on the microelectrode array, cell electrophysiological activity can be monitored for a long time, and the microelectrode array has the advantages of remarkable
cell patterning promotion capability, high stability and robustness, flexible
cell pattern design, excellent biological compatibility and simple process.