Disclosed is a high-accuracy biological 3D (three-dimensional) printing method. According to the method, firstly, procedures for controlling a platform to move are compiled on a computer according to
cell patterns wanting to be printed or three-dimensional
cell gel structures, secondly,
mixed solution of cells / growth factors /
biological materials for 3D printing is selected, the cells / the growth factors / the
biological materials are sucked into a
syringe with a
metal sprayer in a mixed manner, the
metal sprayer is connected with a positive
electrode of direct-current high-
voltage power supply, a receiving plate is fixed on the movable platform below the
metal sprayer and connected with the ground, the
mixed solution forms material jet streams by the aid of electrostatic force, and the movable procedures on the movable platform are started, so that the material jet streams form the predesigned patterns or structures on the receiving plate. According to a cross linking mode, a treatment mode of temperature and
calcium ions is changed, so that the material jet streams rapidly forms cells / hydrogel in situ through illumination. Various fine
cell patterns and three-dimensional cells / gel micro-structural systems can be rapidly printed on a substrate as required.