54 results about "Cell patterning" patented technology

An SRAM array and a dummy cell row structure is discussed that permits an SRAM array to be divided into segments isolated by a row pattern of dummy cells. The dummy cell structure avoids the use of special OPC conditions at the power supply line and block boundaries by providing a continuous cell array at the lower cell patterning levels in an area efficient implementation. In one implementation, the SRAM array comprises a first and second array block each comprising an SRAM cell having a first layout configuration, one or more of the dummy cells having a second layout configuration arranged along the row pattern associated with a wordline of the SRAM array, a first power supply voltage line connected to the first array block, and a second different power supply voltage line connected to the second array block. The first and second power supply voltage lines of the array blocks are further connected to the one or more dummy cells. Beneficially, the bitlines of the array may be continuous across the first and second array blocks and a dummy cell associated therewith.

A method of making a memory cell or magnetic element by using two hard masks. The method includes first patterning a second hard mask to form a reduced second hard mask, with a first hard mask being an etch stop for the patterning process, and then patterning the first hard mask to form a reduced first hard mask by using the reduced second hard mask as a mask and using an etch stop layer as an etch stop. After patterning both hard masks, then patterning a functional layer by using the reduced first hard mask as a mask. In the resulting memory cell, the first hard mask layer is also a top lead, and the diameter of the first hard mask layer is at least essentially the same as the diameter of the etch stop layer, the adhesion layer, and the functional layer.

The present invention provides a masking system for selectively applying cells to predetermined regions of a surface. A mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions of the surface to remain uncovered. Cells then are applied to uncovered portions of the surface and the mask removed. Alternatively, a cell-adhesion promoter is applied to uncovered portions of the surface, and then cells are applied to the surface before or after removal of the mask from the surface. The masking system can be pre-coated, at least on those surfaces which will come into contact with cells, with a cell-adhesion inhibitor to resist absorption of cells and thereby avoid cell damage when the mask is removed (if cells are deposited prior to removal of the mask). A polymeric elastomeric mask that comes into cohesive-conformal contact with a surface to be patterned can be used.

This invention is directed to the use of focused energy, particularly focused acoustic energy, in the spatially directed ejection of cells suspended in a carrier fluid, e.g., for providing a pattern of cells on a substrate surface, such as a cellular array.

This invention describes self assembled monolayers (SAMs) manufactured by imprinting reactive peptides onto solid supports. The invention further relates to methods of preparing and using these improved SAMs.

The invention provides a substrate for cell micropatterning growth. The substrate comprises an inert surface on which cells cannot be adhered and patterns on the inert surface, wherein the patterns are formed from substances which are allowable for cell adhesion, and the inert surface is not completely covered by the patterns. The invention further provides a preparation method of the substrate, wherein a one-step printing step is adopted in the method for preparing the substrate, so that the traditional method for preparing the substrate for the cell micropatterning growth is greatly simplified, and the range for using the substrate is broadened. The invention further provides application of the substrate to the cell micropatterning growth.

Methods are provided for the three dimensional manipulation of cells, and for the formation of an organized engineered cell tissue. Also provided are the organized engineered cell tissues produced by the methods. In one method, a plurality of magnetically labeled cells are mixed with a cross-linkable hydrogel to form a cell-hydrogel mixture, the at least a portion of the plurality of magnetically labeled cells are manipulated with a magnetic field to arrange the magnetically labeled cells into a specific cellular arrangement, and the hydrogel is crosslinked to form the organized engineered cell tissue. The approach presented herein offers a means to circumvent the deficiencies in the field of regenerative medicine, and allows for the production of organized tissues in situ with specific cellular organizations that mimic the native tissue.

Provided is a method for cell patterning, using an electrode substrate including a plurality of electrodes and a cell culture substrate disposed so as to face the electrode substrate, the method comprising the steps of:introducing a cell suspension containing cells into a region between the electrode substrate and the cell culture substrate; applying a voltage to the electrodes to generate a non-uniform electric field in the region; and arranging the cells at a position with low electric field strength on the cell culture substrate by utilizing negative dielectrophoresis so as to obtain the cell culture substrate on which the cells are arranged in a predetermined pattern.

Guided cell patterning arrays for single cell patterning, methods for making the arrays, and methods for using the arrays.

Provided are a hydrogel-encapsulated cell patterning and transferring method comprising: preparing a substrate having a hydrogel-encapsulated cell patterning comprising a first cell and an alginate hydrogel; preparing an agarose hydrogel substrate comprising agarose hydrogel and any one of a second cell and a physiological active substance; and disposing the substrate having the hydrogel-encapsulated cell patterning on the agarose hydrogel substrate and transferring the cell patterning and a biosensor comprising: a first substrate having a hydrogel-encapsulated cell patterning comprising a first cell and an alginate hydrogel; and an agarose hydrogel second substrate comprising agarose hydrogel and any one of a second cell and a physiological active substance.

The present invention provides a method for performing a biological test under conditions in which an artificially prepared cell pattern with initial position coordinates that can be determined is three-dimensionally cultured within a gelled matrix. The present invention relates to a biological test method that comprises testing a biological indicator with reference to at least one selected from the group consisting of cell proliferation, cell movement, and cell differentiation in a cell pattern substantially embedded in gel. The present invention also relates to a kit for the biological test method.

The invention provides a novel microcontact printing system for cell patterning. The system comprises oligomeric dopamine formed by dopamine monomers through polymerization. The molecular weight of the oligomeric dopamine is between 1000 and 10000 D; and a structural formula is shown in the description. A substrate is firstly subjected to chemical modification treatment to obtain a bioadhesion-resistant inert substrate; then, a water solution of the oligomeric dopamine is prepared; a stamp is prepared; next, the stamp is soaked into the water solution of the oligomeric dopamine; and next, thestamp adsorbing the oligomeric dopamine is stamped onto the inert substrate, so that a pattern on the stamp is obtained from the surface of the substrate. The patterning substrate can also be used forcell patterning growth. The realization of the system is simple. The existing technology for cell patterning is simplified.

The invention belongs to the technical field of a 3D printing technique and cell culture and specifically relates to a device and method for quickly performing cell patterning co-culture based on 3D printing. The invention provides a micro device technique for the cell culture and cell patterning co-culture by utilizing 3D printing to quickly prepare. The device can be conveniently compatible with the present cell biological technique, such as, micro-imaging and cell immune staining. The patterning of various cells can be conveniently realized, the cells can be subjected to micro-imaging analysis and immunohistochemical analysis, and a quantification result can be obtained. The device and the method are supplied for researchers to quickly and conveniently realize the co-culture of multiple cells.

The invention discloses a cell patterning structure based on an ordered polymer film, a preparation method and an application in cell proliferation inhibition, belonging to the technical field of cell positioning and assembling. The method comprises the following steps: preparing an ordered porous film the inner wall of which is loaded with a positive charge surfactant, selectively assembling polyelectrolyte with charges which is opposite to that of cells on the inner walls of pores of the ordered porous film, selectively adsorbing inside the pores through the cells, and the like. The cell patterning structure disclosed by the invention is simple and easily available, convenient to operate, gentle in condition, short in period and capable of achieving selective distribution of cells in the ordered honeycomb-shaped film pores, and moreover through selection of cells of appropriate sizes, a single cell array in which each pore only has one cell can be prepared. The single cell array has significant meanings in the fields such as biosensors, tissue engineering, single cell carrying and behavior operation. The structure has significant application values in aspects such as cell transferring and transportation, cell proliferation inhibition and treatment on cancer and other diseases.

The invention pertains to methods and apparatuses for rapid production of organized tissue precursors with different sizes and geometry. An embodiment of the invention provides a method of producing 3D tissue structures, the method comprising: producing a plurality of patterned monolayers of cells and sequentially overlaying the plurality of patterned monolayers of cells to produce a stack of monolayers of cells. The patterns of cells in the plurality of monolayers can be designed in a manner which produces a tissue or a portion of a tissue when the plurality of monolayers is overlaid in to a stack. The invention also pertains to an apparatus for producing a 3D tissue module. The apparatus comprises: a three-dimensional positioning system, a contact stamp, a contact stamp storage container, a contact stamp holder, a contact stamp exchange unit, and a contact stamp actuator.

The invention provides a manufacturing method of a polyacrylamide gel substrate with a single-cell pattern array. The manufacturing method comprises the following steps: manufacturing cover glass with a single-cell micro-pattern array on the surface; carrying out hydrophilization treatment on the glass substrate at the bottom of the culture dish; adding a polyacrylamide prepolymer solution containing fluorescent particles into the culture dish subjected to hydrophilization treatment, imprinting the polyacrylamide prepolymer solution by adopting the cover glass with the single-cell micro-pattern array on the surface, and after the polyacrylamide solution is solidified, taking out the single-cell micro-pattern array on the surface of the cover glass, so as to obtain the single-cell micro-pattern array on the surface of the single-cell micro-pattern array. The polyacrylamide gel substrate containing the artificial speckles and the single-cell pattern array is obtained. The single-cell pattern array is formed on the polyacrylamide gel substrate through the micro-contact printing technology, the polyacrylamide gel substrate with the single-cell pattern array on the surface is manufactured, the method has the advantage that a large number of cells are regularly arranged in a single device, preparation is easy and convenient to implement, one-time forming is achieved, redundant operation is not needed, and the method is suitable for large-scale production. And a good basis is provided for high-throughput single-cell force spectrum testing.

The invention discloses a full liquid phase organ chip and a preparation method thereof. The full liquid phase organ chip comprises a super hydrophobic porous membrane with a hydrophilic channel, theporous membrane is sealed in an oil phase, the hydrophilic channel is provided with a fluid inlet and a fluid outlet, and the fluid inlet and the fluid outlet communicate with the hydrophilic channel;the polymer layer porous membrane is placed in a functional modification solution to obtain a hydrophobic polymer layer porous membrane; hydrophilic patterns are treated on the hydrophobic polymer layer porous membrane; then, polymer surface superhydrophobic treatment is carried out on the surface of the porous membrane with the hydrophilic channel; and then the hydrophilic channel on the surfaceof the porous membrane is wetted and sealed in the oil phase, and an inlet and an outlet which communicate with the hydrophilic channel are formed. According to the full liquid phase organ chip and the preparation method thereof, the preparation conditions are mild, the whole liquid phase organ chip can be constructed by constructing cell patterned culture on a prepared substrate, and the in-situdetection and sampling of all micro environments in the organ chip can be realized, and the spatial editing of the function and geometric layout of a fluid device can be realized as required.

The present invention provides a method for performing a biological test under conditions in which an artificially prepared cell pattern with initial position coordinates that can be determined is three-dimensionally cultured within a gelled matrix. The present invention relates to a biological test method that comprises testing a biological indicator with reference to at least one selected from the group consisting of cell proliferation, cell movement, and cell differentiation in a cell pattern substantially embedded in gel. The present invention also relates to a kit for the biological test method.

The invention discloses an avidin-biotin system cell patterning-based chip and preparation and applications thereof, belonging to the technical field of a biological micro system. The method comprises the steps: preparing a bioaffinity hexamethyl disilazane (HMDS) lattice array and bio-passivated polyethylene glycol pattering area by adopting photoetching and chemical precipitation methods; then forming a sandwich structure of biovine serum albumin-biotin, avidin and biotinylation cells on the bioaffinity HMDS lattice array, thereby directionally distributing cells and realizing the separation and locating of cells. The materials used in the method are simple, and the method is simple and convenient; the distribution of the cell arrays can be realized by controlling the size and distribution mode of the HMDS; the method is used for patterning various cells; single-cell arrays are not easily broken in the following detection and application of the chip; and the materials and biochemical substances have small influence on the activity of cells, thereby being used for detecting living cells.

Apparatus for single cell patterning, the apparatus comprising: a structure comprising a surface channel formed therein, the surface channel being connected to an inlet and an outlet; and a cell trap disposed in the surface channel, the cell trap comprising a body defining a flow diverter for diverting flow passing by the cell trap into a wide path or a narrow path, and the body and the structure together defining a well for capturing a cell diverted by the flow diverter toward the narrow path.

The invention provides an application of tannic acid serving as micro-contact printing ink to cell patterning. According to the application, firstly, a substrate is subjected to polyethylene glycol surface treatment to obtain an 'inert' substrate; secondly, water solution of tannic acid is prepared, and a seal is prepared; thirdly, the seal is soaked into the water solution of the tannic acid; finally, the seal adsorbing the tannic acid is covered on the 'inert' substrate, so that a pattern on the seal is acquired on the surface of the substrate. The pattern is transparent and can chelate ferroferric oxide nano-particles, so that the pattern is visual, the patterned substrate can further be used for patterned growth of cells, a patterned method is simple to operate, and an existing cell patterning technique is simplified.

A method for producing a cell pattern is provided, comprising the step of forming a hydrophobic film on a substrate, patterning the hydrophobic film by a laser beam, transferring the hydrophobic film from the substrate to a medium, and culturing cells on the medium to form a cell pattern.