Manufacturing method of polyacrylamide gel substrate with single cell pattern array

Abstract

The invention provides a manufacturing method of a polyacrylamide gel substrate with a single-cell pattern array. The manufacturing method comprises the following steps: manufacturing cover glass with a single-cell micro-pattern array on the surface; carrying out hydrophilization treatment on the glass substrate at the bottom of the culture dish; adding a polyacrylamide prepolymer solution containing fluorescent particles into the culture dish subjected to hydrophilization treatment, imprinting the polyacrylamide prepolymer solution by adopting the cover glass with the single-cell micro-pattern array on the surface, and after the polyacrylamide solution is solidified, taking out the single-cell micro-pattern array on the surface of the cover glass, so as to obtain the single-cell micro-pattern array on the surface of the single-cell micro-pattern array. The polyacrylamide gel substrate containing the artificial speckles and the single-cell pattern array is obtained. The single-cell pattern array is formed on the polyacrylamide gel substrate through the micro-contact printing technology, the polyacrylamide gel substrate with the single-cell pattern array on the surface is manufactured, the method has the advantage that a large number of cells are regularly arranged in a single device, preparation is easy and convenient to implement, one-time forming is achieved, redundant operation is not needed, and the method is suitable for large-scale production. And a good basis is provided for high-throughput single-cell force spectrum testing.

Description

technical field [0001] The present disclosure relates to the technical field of cell mechanics measurement, and in particular to a method for making a polyacrylamide gel substrate with a single-cell pattern array on its surface based on microcontact printing technology. Background technique [0002] In recent years, more and more people pay attention to the study of the mechanical environment in which cells live. In organisms, the connection and interaction between cells and extracellular matrix constitute the mechanical environment for the survival and growth of most cells. Cell traction refers to the interaction force between cells and the surrounding environment, and cell adhesion, spreading and migration are all driven by cell traction. Many important physiological activities, such as embryonic development, wound healing and immune response, all require the action of cell traction. [0003] The measurement and research of cell traction mainly rely on Traction Force Mic...

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Problems solved by technology

[0005] In the traditional cell traction force microscopy technique, most of the cell culture devices used use the cover glass or the glass substrate of the confocal culture dish as the support substrate of the polyacrylamide gel, which has a low throughput when performing large-scale experiments. , long production time, complex operation process and other defects, and mass production of flexible substrate petri dishes, it is difficult to ensure that the difference is minimized, and the impact on the stability of the experiment is difficult to ignore

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