26 results about "Cell mechanics" patented technology

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

The invention belongs to the technical field of biological image analysis and cell mechanics research, and particularly relates to a cell intracellular mechanical response visual analysis method based on image feature extraction, which comprises the following steps: acquiring original image data including a dynamic change process of cells under mechanical stimulation; performing structured segmentation on the original image data; extracting morphological features and dynamic behavior features of each data unit to obtain feature elements; classifying the feature elements according to a preset biological significance or mechanical response mode to form a plurality of feature element groups; and based on the classified feature element group, constructing a mapping relationship between the cell local region or the time slice and the mechanical response. According to the invention, the mechanical response of cells in a local area and a specific time window under mechanical stimulation can be analyzed, so that an analysis basis is provided for cell mechanical mechanism research, differentiation process regulation and control, drug effect evaluation and the like.

The invention relates to the technical field of biological materials, and discloses electrical stimulation LDH hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a zinc salt, a vanadium salt and urea in water in a nitrogen atmosphere to obtain a precursor solution; the precursor solution is subjected to a hydrothermal reaction, and ZnV-LDH is obtained; under stirring, gradually dropwise adding methacrylic anhydride into the gelatin solution, and heating for reaction; adding preheated water into the reaction liquid to stop the reaction; dialyzing and purifying the reaction liquid, pre-freezing, and freeze-drying to obtain hydrogel; and adding ZnV-LDH into the hydrogel, and carrying out ultrasonic mixing, so as to obtain the electrical stimulation LDH hydrogel. Through the synergistic effect of the ZnV-LDH and the hydrogel, the electrical stimulation LDH hydrogel disclosed by the invention has electrical activity and can generate potential change in response to external stimulation; under ultrasonic stimulation, concentration-dependent electrical response can be generated, the response has periodicity and stability, and osteogenic differentiation and cell mechanical properties can be promoted to the maximum extent.

A cellular mechanical property measurement and sorting method comprises: cells to be sorted being arranged in a straight line after passing through a standing wave acoustic field generated by a first interdigital transducer; the cells flowing through a focused acoustic field generated by a second interdigital transducer, and the focused acoustic field generating a radiation force on the cells, such that the cells are deformed; performing calculation according to information of the radiation force and deformation, so as to obtain elastic moduli of the cells; determining the positions of the cells, and selectively driving, with designed delay time, interdigital transducers in a cell sorting unit according to the moving speeds of the cells and the distances between the cells and the sorting unit, and triggering, according to the elastic moduli, a corresponding interdigital transducer to generate a planar acoustic field, so as to sort the cells.

The invention discloses an integrated chip for mechanical fatigue damage and mechanical characterization of red blood cells, and particularly relates to the crossing field of a micro-fluidic technology and cell mechanics. Comprising a substrate, and a PDMS functional layer is arranged on the substrate; the substrate and the PDMS functional layer are tightly bonded to form a composite micro-channel system with bionic flow field characteristics, the composite micro-channel system is composed of a fatigue loading structure, a fluid buffer structure and a bionic detection structure which are sequentially communicated, the fatigue loading structure is composed of straight micro-channels and arc-shaped flow channels which are alternately communicated, the fluid buffer structure adopts a taper gradually-shrinking design, and the bionic detection structure adopts a bionic detection structure. A plurality of orthogonally arranged micro-column arrays are arranged in the bionic detection structure and the fatigue loading structure. By adopting the technical scheme, the problems of mechanical environment distortion and insufficient observation resolution of a traditional method are solved, and a standardized platform is provided for mechanical property evaluation and clinical pathological diagnosis of red blood cells under health and fatigue damage.

The invention relates to an intelligent cell mechanical property analysis method driven by a cell queue flow mechanism, which comprises the following steps of: acquiring experimental group flow data generated when a cell queue flows in a micro-fluidic channel to realize non-intrusive and high-throughput experimental observation and data capture of cell individuals and group flow dynamics; and an experiment reference is provided for analysis. A cell queue fluid-solid coupling model is established, numerical simulation is carried out, multiple groups of simulation group flow data are obtained by changing mechanical parameter values, complex flow behaviors of a cell queue under various potential mechanical characteristics are efficiently and controllably simulated in a virtual environment, and a simulation database with sufficient samples is established. Experimental data of a queue to be tested are matched with simulation data in a three-dimensional simulation database, so that the complex flow performance of a sample to be tested is quickly compared with the simulation database of massive known cell types and mechanical parameters, complex modeling and parameter inversion processes are bypassed, and the most similar known type is directly positioned.

The invention relates to the technical field of microfluidics, in particular to a microfluidics system, a method for conveying a target object and an elastic modulus detection system and method.The microfluidics system comprises a device and an air extractor, the device is provided with at least one micro-channel, and the air extractor is used for extracting air from the device, so that the air pressure in the micro-channel is smaller than the ambient air pressure. The high solubility of the device provided with at least one micro-channel to gas is utilized, and energy required for pumping liquid is pre-stored in the degassed device by utilizing the air extractor, so that the device can realize self-driven transportation of the cell suspension and enable the cell suspension to deform through the slit channel under the condition that external source pump equipment does not need to be built. The self-transportation process of the cell suspension is realized by utilizing deformation pre-stored energy generated by a device under vacuum and generating pressure difference when the vacuum is released, so that the traditional fluid driving equipment is saved, the operation process is simplified, the system cost is reduced, and a feasible path is provided for high-throughput and low-threshold detection of cell mechanical properties.

This invention discloses a vascular organ-on-a-chip that simulates human blood pressure, comprising a substrate, a channel layer, a cell dynamic culture module, a solution mixing module, and a cell mechanical property measurement module. The cell dynamic culture module includes a first culture channel and a second culture channel, with a serpentine channel connecting the upstream of the first culture channel and the upstream of the second culture channel. The solution mixing module includes two oppositely arranged solution mixing channels, which are respectively connected to the first culture channel and the second culture channel. The cell mechanical property measurement module includes multiple measurement channels arranged side-by-side, with one end of each measurement channel connected to the other end of one solution mixing channel and the other end connected to the other end of another solution mixing channel. This invention simulates the human blood pressure environment within the vascular organ-on-a-chip, enabling simultaneous cell culture under parallel gradient pressure within the chip, and combining the vascular organ-on-a-chip with cell mechanical property measurement.

This specification provides a control method and apparatus for a cell mechanical property measurement and sorting system. Based on this method, the control module first controls a camera to acquire target images of the target cells in a matching manner according to the operating parameters of the first interdigital transducer, obtaining relatively good target images. Then, the control module performs a basic first processing on the target images, with relatively small data processing volume, to quickly obtain the first processing result. Based on the first processing result, the second interdigital transducer is controlled to perform corresponding cell sorting processing. Simultaneously, the target images are sent to a computer device via a PCIe interface, utilizing the computer device's data processing resources to perform a more refined second processing on the target images, with a relatively larger data processing volume, obtaining the second processing result. This effectively reduces system latency and efficiently and accurately controls the corresponding modules in the system to cooperate in completing the cell sorting process.

The invention provides construction and application of an intermolecular structural domain tension probe, the probe comprises a first structure, a second structure and a fluorescent protein pair eCFP and eYFP, the first structure and the second structure are respectively connected to two ends of the fluorescent protein pair, and the eCFP and the eYFP are connected through a short peptide; the first structure is a structural domain or protein capable of being combined with a cytoskeleton connecting protein, and the second structure is a structural domain or protein capable of being combined with a cytoskeleton; the cytoskeleton connecting protein is selected from talin, SYNE4, SYNE3, SYNE2, Desmoplakin and the like; the cytoskeleton is a microfilament, a microtubule or an intermediate filament. The intermolecular structural domain tension probe can be used for detecting intracellular mechanical signals, the limitation of a traditional tension probe is overcome, the adverse effect of function enhancement caused by gene cloning is avoided, a new opinion is provided for a mechanical transduction molecular mechanism, and the application has the advantages that the application range is wide, and the application prospect is wide. And a more accurate experimental tool is provided for the research of cell mechanics biology, and the effect of a mechanical signal in cell behavior regulation and control is disclosed.

The invention discloses a working method of an in-vitro simulation system for reproducing a mechanical microenvironment at an internal arteriovenous fistula, and belongs to the technical field of cell mechanical biology experimental devices for health and rehabilitation engineering. A real wall shear stress waveform is obtained based on clinical blood vessel data of an arteriovenous fistula stenosis patient in combination with computational fluid mechanics simulation, quantitative calculation of shear stress is achieved by means of a fluid mechanics simplified formula of a parallel plate flow cavity, and accurate loading of pulsation, oscillation and other forms of shear stress is adapted by means of quasi-steady hypothesis. The wall shear stress microenvironment of the arteriovenous fistula anastomosis position in different physiological and pathological states can be reproduced in the flowing cavity, and meanwhile, the mechanical and biological response indexes of endothelial cells can be quantitatively monitored in real time. According to the method, the real mechanical microenvironment of the vascular endothelial cells at the arteriovenous fistula is highly restored, and a miniaturized, standardized and quantitative experimental platform is provided for researching the quantitative relation between the wall shear stress signal of the area and the mechanical biological effect of the vascular endothelial cells.

The invention discloses a system and a method for controlling an'optical handle 'to perform cell excitation regulation based on a scanning Gaussian beam, and belongs to the technical field of optics. The system comprises a lighting source, an objective table, an inverted fluorescence microscope, a dichroscope group, a galvanometer, a spatial light modulator, a 1064nm laser, a fluorescence LED light source and a CMOS camera. The method comprises the following steps: preparing a 3-micron polystyrene microsphere as an optical handle, and culturing K562 cells; a scanning Gaussian beam is controlled by a galvanometer to drive an optical handle to rotate; the method comprises the following steps: optically assembling a target cell and a rotating optical handle in a three-dimensional space, and driving the cell to rotate to apply mechanical stimulation; and the cell calcium ion signal change is monitored in real time by utilizing fluorescence imaging. According to the invention, continuous, stable and controllable rotary stimulation on cells is realized, high-throughput parallel control, high three-dimensional space resolution and real-time dynamic monitoring capability are realized, and a brand new tool is provided for cell mechanics research.

The invention discloses a mechanical phenotype analysis chip for screening bacterial infected circulating tumor cells, and belongs to application of a micro-fluidic chip technology in the field of biomedical in-vitro diagnosis. The system comprises a micro-fluidic chip and a detection solution. The micro-fluidic chip comprises a sample introduction end, a shunting flow channel, a main flow channel and a sample discharge end, wherein single-cell mechanical property analysis function unit arrays are uniformly distributed on the two sides of the main flow channel in a staggered manner; and the single cell mechanical property analysis functional unit comprises a hook-shaped structure for single cell anchoring and a deformation channel structure for single cell mechanical property analysis. The detection solution comprises a fluorescent probe capable of specifically recognizing bacteria in cells. According to the micro-fluidic chip disclosed by the invention, single cell capture, mechanical property analysis and intracellular bacteria detection can be integrated on the same chip, and a time-saving, low-cost and high-throughput single cell detection platform can be constructed, so that visual detection of single cell mechanical phenotypes and intracellular bacteria is realized; the method can be used for evaluating the metastasis potential of the circulating tumor cells in the bacterial infection breast cancer.

Cytoskeletal Network Based Computer Device for Predicting Tissue Cell Mechanics

The application discloses a micro-fluidic device and a method for analyzing cell mechanical properties by using the micro-fluidic device. The micro-fluidic device comprises a plurality of micro-channels, each of which is provided with a normal region through which simulated cells to be measured can easily pass and a narrow region through which the simulated cells to be measured are not easy to pass, and the size of the micro-channels meets the size range of micro-arteries. The application has the advantages of simple structure of a measuring and analyzing device and easy execution of a measuring and analyzing method.

The present application belongs to the technical field of biological image analysis and cell mechanics research, and particularly relates to a cell intracellular mechanics response visual analysis method based on image feature extraction, comprising the following steps: acquiring original image data containing dynamic change process of cells under mechanical stimulation; structurally segmenting the original image data; extracting morphological features and dynamic behavior features for each data unit respectively to obtain feature elements; classifying the feature elements according to preset biological significance or mechanical response mode to form several feature element groups; and constructing a mapping relationship between local regions or time segments of cells and mechanical responses based on the classified feature element groups. The present application can analyze the mechanical response of local regions and specific time windows of cells under mechanical stimulation, thereby providing analysis basis for cell mechanics mechanism research, differentiation process regulation, drug effect evaluation and the like.

The invention belongs to the technical field of cell mechanics, and particularly relates to a single cell dynamic mechanical loading system based on magnetic beads and a bidirectional static magnetic field and a force regulation and control method. The invention provides a single cell dynamic mechanical loading system based on magnetic beads and a bidirectional static magnetic field and a force regulation and control method. The system is composed of a magnetic field system module, a dynamic regulation and control module and a cell stress feedback module. Through the system, single-cell-level pulling / pressing two-way mechanical stimulation is achieved for the first time, magnetic bead concentration-magnetic field direction cooperative adjustment is achieved, and limitation of a traditional multi-cell loading mechanical mode is broken through; the system is used for carrying out mechanical regulation and control on single cells, can be used for single cell mechanical response research (such as stem cell differentiation and tumor cell migration), drug screening (detecting the influence of drugs on cell mechanical characteristics) and construction of a dynamic mechanical microenvironment in tissue engineering, and has a good application prospect.

The application relates to the technical field of biological materials, and discloses an electrically stimulated LDH hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a zinc salt, a vanadium salt and urea in water under a nitrogen atmosphere to obtain a precursor solution; performing a hydrothermal reaction on the precursor solution to obtain ZnV-LDH; gradually adding methacrylic anhydride into a gelatin solution under stirring, heating and reacting; adding preheated water into the reaction solution to stop the reaction; performing dialysis purification, pre-freezing and freeze-drying on the reaction solution to obtain a hydrogel; and adding the ZnV-LDH into the hydrogel, ultrasonic mixing, and obtaining the electrically stimulated LDH hydrogel. Through the synergistic effect of the ZnV-LDH and the hydrogel, the electrically stimulated LDH hydrogel has electric activity, can respond to external stimulation to generate a potential change, can generate a concentration-dependent electric response under ultrasonic stimulation, and the response has periodicity and stability, so that the osteogenic differentiation and the cell mechanical properties can be promoted to the maximum extent.

The invention relates to the technical field of biomedical engineering and cell mechanical stimulation, in particular to a cell positive and negative pressure and mechanical stimulation culture device which comprises a shell, and supporting legs are fixedly connected to the four corners of the bottom of the shell; the storage battery is fixedly arranged in the shell; the controller is fixedly connected in the shell; the placing assembly is arranged in the shell in a sliding manner; the adjusting assembly is arranged at the upper end in the shell; the connecting plate is slidably connected to the middle end in the shell, the side wall of the connecting plate is fixedly connected with the moving end of an electric push rod, and the electric push rod is fixedly connected to the top in the shell. The negative-pressure pump and the booster pump are connected with the negative-pressure box and the booster box respectively, in cooperation with precise control of the first electromagnetic valve and the second electromagnetic valve, the negative-pressure and positive-pressure environments of a culture chamber in the culture dish can be rapidly and independently adjusted, a pressure sensor in a test hole collects data in real time, closed-loop control is formed, and it is ensured that pressure parameters are stable; and the mechanical stress borne by the cells in vivo is simulated.

The utility model belongs to the technical field of cell mechanics biology experimental devices for health and rehabilitation engineering, and relates to a microfluidic device for endothelialization evaluation of a tissue engineering valve, which comprises a microfluidic chip, peripheral equipment, a signal acquisition and processing system and a feedback system. According to the utility model, the biomechanical principle and the micro-fluidic chip technology are ingeniously combined, the control system, the mechanical loading device and the micro-fluidic chip are connected to construct a micro-fluidic endothelial cell culture cavity and a peripheral system, and the real-time accurate control of the blood flow of the aortic valve is realized through the PID control system; shear stress applied to a tissue engineering valve endothelial cell culture area is generated, so that the real stress condition of the tissue engineering valve endothelial cells is simulated, and the method is used for analyzing and researching the influence rule of mechanical loading on the tissue engineering valve endothelial cells.

1. Name of the product in this design: Microfluidic chip for single-cell extrusion. 2. Purpose of this design: For single-cell mechanical research. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present application relates to a kind of real-time monitoring method for realizing signal molecule release in single cell deformation process by optical tweezers manipulation combined microelectrode sensing, comprising the following steps: obtaining ITO film microelectrode of electrically active area that can only accommodate one cell size;Preparation of working electrode that can specifically respond to signal molecule concentration change;With working electrode as bottom, build electrochemical chamber of three electrode system;With biomolecule modification microsphere carrier, and place it in electrochemical chamber;Two microsphere carriers are captured respectively by double optical trap optical tweezers and contacted with cell, and cell is stretched or extruded in working electrode area to deform, and the force spectrum information and deformation variable in cell deformation process are determined;Signal molecule concentration change released in cell deformation process is monitored in real time using electrochemical workstation.The present application can be used to study the relationship between the change of single cell release chemical information and cell mechanical information under mechanical force stimulation.

The invention discloses a method for promoting CAR (chimeric antigen receptor) aggregation by using DNA domino effect, and relates to the technical field of crossing of immunotherapy and biomechanics, the method comprises a trigger chain (Tr), a hairpin structure (HP1 / HP2) and a CAR-T cell, a multivalent DNA network structure is formed on the surface of the CAR-T cell through a hybridization chain reaction, the aggregation state of a CAR molecule is dynamically regulated, the trigger chain (Tr) is a single-stranded DNA molecule, and the hairpin structure (HP1 / HP2) is a hairpin structure (HP1 / HP2). CAR can be specifically recognized and a cascade hybridization reaction of a hairpin structure (HP1 / HP2) can be initiated, the hairpin structure (HP1 / HP2) is a partially complementary DNA sequence, a linear or branched DNA polymer is formed through the HCR reaction, and a CAR aggregation signal is expanded. According to the method for promoting CAR aggregation by using the DNA domino effect, controllable adjustment of CAR aggregation is realized through HCR, and mechanical properties of target cells are responded; and the DNA network structure obviously improves the mechanical force conduction efficiency of the CAR-T cells.

This invention provides a method for evaluating stem cell trilineage differentiation based on single-cell membrane tension measurement, comprising: measuring the membrane tension of multiple single cells in a stem cell sample to be tested, and calculating the mean membrane tension; setting a membrane tension threshold for stem cell trilineage differentiation, comparing the mean membrane tension with the threshold, and evaluating the differentiation process and efficiency of the stem cell sample to be tested. This invention also provides the application of the above evaluation method in the quality control of clinical-grade stem cells, the evaluation of stem cell differentiation induction effects, or the screening of stem cell virulence before transplantation. This invention utilizes optical tweezers technology to construct a single-cell mechanical testing platform, enabling non-destructive quantitative measurement of single-cell membrane tension. Cells can remain intact and viable after testing and can be reused. By comparing the membrane tension data of the sample to be tested with the threshold, a systematic evaluation of stem cell differentiation process and efficiency can be completed, which can be widely applied in the fields of stem cell quality control and regenerative medicine clinical translation.

The invention relates to the technical field of cell detection and analysis, and particularly discloses a microfluidic detection system and method for erythrocytes of diabetic patients. The system comprises a PDMS micro-fluidic chip, an equal-diameter bionic micro-channel array, a closed-loop constant-pressure pump and a high-speed imaging module. Synchronous observation of dynamic flow deformation behaviors of red blood cells is realized by simulating a diabetes microvessel shearing environment and combining dark field illumination and a Canny edge detection algorithm. The method comprises the steps of blood sample anticoagulation treatment, erythrocyte suspension gradient centrifugal purification, dynamic flow deformation analysis under pulsating pressure driving and scanning electron microscope morphological characterization. The system breaks through the limitation of the traditional technology, can effectively, losslessly and rapidly measure the flow deformation characteristics of erythrocytes of diabetic patients under different glycosylated hemoglobin concentrations, has high-throughput and non-contact cell mechanical property detection characteristics, has low experience requirements on operators, and has high stability and repeatability.

The invention discloses a high-throughput real-time detection method and system for cell rotation dynamics based on yolov8, and belongs to the technical field of biological detection and single cell analysis. The system comprises an image acquisition module and an information processing module. The core of the invention lies in that the information processing module is configured to receive an image sequence containing a plurality of rotating cells, perform automatic identification and accurate positioning on unmarked cells by using a pre-trained deep learning instance segmentation model, allocate a unique ID for each cell through a multi-target tracking algorithm, and send the unique ID to the image sequence; and the rotation speed of each cell is calculated in parallel based on an image similarity comparison method. According to the method, the defects of low flux, poor efficiency and dependence on manpower in the prior art are overcome, real-time, quantitative and statistical analysis of rotation information of the cell population is realized, and the mechanical heterogeneity of the cell population can be effectively revealed. The system provides a powerful tool for drug high-throughput screening, disease diagnosis models and cell mechanical property research.