5 results about "Cell mechanics" patented technology

A cellular mechanical property measurement and sorting method comprises: cells to be sorted being arranged in a straight line after passing through a standing wave acoustic field generated by a first interdigital transducer; the cells flowing through a focused acoustic field generated by a second interdigital transducer, and the focused acoustic field generating a radiation force on the cells, such that the cells are deformed; performing calculation according to information of the radiation force and deformation, so as to obtain elastic moduli of the cells; determining the positions of the cells, and selectively driving, with designed delay time, interdigital transducers in a cell sorting unit according to the moving speeds of the cells and the distances between the cells and the sorting unit, and triggering, according to the elastic moduli, a corresponding interdigital transducer to generate a planar acoustic field, so as to sort the cells.

This invention discloses a vascular organ-on-a-chip that simulates human blood pressure, comprising a substrate, a channel layer, a cell dynamic culture module, a solution mixing module, and a cell mechanical property measurement module. The cell dynamic culture module includes a first culture channel and a second culture channel, with a serpentine channel connecting the upstream of the first culture channel and the upstream of the second culture channel. The solution mixing module includes two oppositely arranged solution mixing channels, which are respectively connected to the first culture channel and the second culture channel. The cell mechanical property measurement module includes multiple measurement channels arranged side-by-side, with one end of each measurement channel connected to the other end of one solution mixing channel and the other end connected to the other end of another solution mixing channel. This invention simulates the human blood pressure environment within the vascular organ-on-a-chip, enabling simultaneous cell culture under parallel gradient pressure within the chip, and combining the vascular organ-on-a-chip with cell mechanical property measurement.

This specification provides a control method and apparatus for a cell mechanical property measurement and sorting system. Based on this method, the control module first controls a camera to acquire target images of the target cells in a matching manner according to the operating parameters of the first interdigital transducer, obtaining relatively good target images. Then, the control module performs a basic first processing on the target images, with relatively small data processing volume, to quickly obtain the first processing result. Based on the first processing result, the second interdigital transducer is controlled to perform corresponding cell sorting processing. Simultaneously, the target images are sent to a computer device via a PCIe interface, utilizing the computer device's data processing resources to perform a more refined second processing on the target images, with a relatively larger data processing volume, obtaining the second processing result. This effectively reduces system latency and efficiently and accurately controls the corresponding modules in the system to cooperate in completing the cell sorting process.

The application relates to the technical field of biological materials, and discloses an electrically stimulated LDH hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a zinc salt, a vanadium salt and urea in water under a nitrogen atmosphere to obtain a precursor solution; performing a hydrothermal reaction on the precursor solution to obtain ZnV-LDH; gradually adding methacrylic anhydride into a gelatin solution under stirring, heating and reacting; adding preheated water into the reaction solution to stop the reaction; performing dialysis purification, pre-freezing and freeze-drying on the reaction solution to obtain a hydrogel; and adding the ZnV-LDH into the hydrogel, ultrasonic mixing, and obtaining the electrically stimulated LDH hydrogel. Through the synergistic effect of the ZnV-LDH and the hydrogel, the electrically stimulated LDH hydrogel has electric activity, can respond to external stimulation to generate a potential change, can generate a concentration-dependent electric response under ultrasonic stimulation, and the response has periodicity and stability, so that the osteogenic differentiation and the cell mechanical properties can be promoted to the maximum extent.