202 results about "Cell trapping" patented technology

Microfluidic devices and methods that use cells such as cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described. Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible fluidics, inertial fluidics are used as the basis for cell and molecular processing (e.g., characterizing, sorting, isolation, processing, amplification) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically this invention discloses a few sorting schemes for stem cells, whole blood and circulating tumor cells and also extracting serum from whole blood. Further medical diagnostics technology utilizing high throughput single cell PCR is described using immiscible fluidics couple with single or multi cells trapping technology.

A device for individually analysing cells of interest, comprising (a) a channel for receiving the contents of a cell of interest, wherein the channel has an input end and an output end, and (b) a cell trapping site in proximity to the input end of the channel, wherein (i) the input end of the channel is adapted such that an intact cell of interest cannot enter the channel; and (ii) the channel contains one or more analytical components for analysing the contents of the cell of interest. In use, a cell is applied to the device, where it is trapped by the cell trapping means. The cell cannot enter the channel intact, but its contents can be released in situ to enter the channel's input end. The contents can then move down the channel, towards the output end, and they encounter the immobilised reagents, thereby permitting analysis of the cell contents.

The invention relates to a micro-fluidic chip and a micro-fluidic chip system for single cell analysis and a single cell analyzing method. The micro-fluidic chip for single cell analysis comprises a transparent top cover, a microfluid channel, a substrate with a micropore array and a bottom plate with a cavity body, which are sequentially connected from top to bottom in a sealing manner, wherein the transparent top cover is provided with an inflow hole and an outflow hole which are used for communicating the outside and the microfluid channel, the micropore array of the substrate is arranged below the microfluid channel and is communicated with the microfluid channel, each micropore is internally provided with an electrode, each electrode is provided with a cathode and an anode, each micropore can contain a single cell only, the bottom of each micropore is provided with a channel which is communicated with the cavity body of the bottom plate, and the cross section of the channel is smaller than the size of each single cell. With the adoption of the micro-fluidic chip, the micro-fluidic chip system and the single cell analyzing method, the capturing, identifying and disruption processes of each single cell are integrated on one micro-fluidic chip; as a corresponding operation method is provided, the content of each single cell is rapidly acquired and accurately analyzed.

Aiming at the lack of an integrated micro-fluidic chip capable of realizing capture, culture and batch differential administration of single cells simultaneously in the prior, the invention provides an integrated micro-fluidic chip and system for capture, culture and administration of single cells and belongs to application of micro-fluidic chip technologies to the field of cell pharmacology analysis. The integrated micro-fluidic chip comprises a cell capture and culture unit, a drug diffusion unit and a drug supply unit, which are sealed in sequence from top to bottom; the system employing the integrated micro-fluidic chip comprises the integrated micro-fluidic chip, a fluorescence microscope, image processing equipment and a digital injection pump. A preparation method of the integrated micro-fluidic chip comprises the following steps: photo-etching baseplate plane figures on the silicon plates of the three units to etch grooves, pouring a liquid chip material into the grooves, and obtaining the three units after the liquid chip material is solidified; conducting sealing bonding and punching to obtain the integrated micro-fluidic chip. The integrated micro-fluidic chip provided by the invention integrates the three functions of capture, culture and batch differential administration of single cells and effectively avoids sample contamination.

Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.

An improved microfluidic device and method of using the microfluidic device to measure natural motile response of a living moiety to a chemotactic agent. The invention comprises integrating microfluidic containment trenches within the microfluidic devices for cellular trapping, manipulation and real-time analysis of biological phenomena, including chemotaxis. The invention captures the design methodology for these traps, as well as the fabrication means and the associated external control mechanism that allows rapid, reversible and fully controlled changes in the chemical environment to which trapped cells are exposed.

The invention discloses a microfluidic cell-sorting chip integrated with single-cell capture, belongs to the field of microfluidic chips and provides a microfluidic chip capable of being used for detection and diagnosis of leukemia without damaging the activity of leukemia cells. The microfluidic cell-sorting chip is characterized in that the microfluidic cell-sorting chip comprises a substrate and a cell capturing device, wherein an electrode pair is sputtered on the substrate; the cell capturing device is provided with a sample inlet and two sample outlets; the cell capturing device comprises an upper capturing layer and a lower capturing layer which are aligned and bonded to form a cell capturing array and a microfluidic channel; the cell capturing array comprises a plurality of isomorphous single-cell capturing structures; each single-cell capturing structure comprises a U-shaped column and a cylinder; the sample inlet is connected with the head end of the microfluidic channel after penetrating through the upper capturing layer; the microfluidic channel is divided into two paths, and each path is connected with the front end of one cell capturing array; the microfluidic channelconnected with the rear end of the cell capturing array is connected with the sample outlets penetrating through the upper capturing layer. The microfluidic cell-sorting chip can be used for the field of cell sorting.

The invention provides a liquid-drop microfluidic chip for separation of single cells and a preparation method for the liquid-drop microfluidic chip. The chip consists of two layers, i.e., an upper layer and a lower layer, wherein the upper layer is a liquid drop generating unit, and the lower layer is a liquid drop trapping unit; and the liquid drop generating unit is provided with structures asfollows: a dispersed phase inlet (1), a continuous phase inlet (2), a dispersed-phase liquid inlet passage (3), a continuous-phase liquid inlet passage (4), a liquid drop generating cross passage (5),a liquid outflow passage (6), a liquid outlet (7) and a liquid storage tank (8). According to the chip, an SU-8 chip template with partial-bulging passages is prepared by adopting a photoetching andcorroding method and is subjected to stripping by polydimethylsiloxane (PDMS), thereby obtaining a PDMS chip. The chip has the flexible liquid drop generating unit and has the high-pass single-cell trapping unit; and the chip is simple in structure, convenient in operation, high in efficiency, low in consumption of cells and reagents and extensive in range of application.

The invention relates to a microarray-type structure chip adopting micro-flow pipes and taking advantage of diameter difference of different types of cells, and micro column arrays with different intervals and different sizes are etched in the micro-flow pipes. The chip structure is formed by up-down bonding of a silicon Si material negative-film substrate and a soda-lime glass upper cover. According to the different intervals of the column micro structure, the microarray-type structure chip can be divided into two series: variable interval and fixed interval; and according to the different sizes of the column micro structure, the microarray-type structure chip can be divided into a plurality of types: ranging from 2-30 [mu] m. The microarray-type structure chip is characterized in that: when a liquid fluid containing the different types of cells flows through the chip micro structure, and passes through the column microarrays with different intervals and diameters, different types (different sizes) of cells can be captured by different micro column array structures, and retained on the different micro column arrays with different micro structures in the pipes, and the captured results can be observed and analyzed by used of a microscope. The microarray-type structure chip has the characteristics of micro volume, flexible and customizable structure, no moving parts and the like, and can be widely applied to capture and analysis and other fields of various cancer cells in the blood, and comprehensive analysis and measuring and calculating determine of the cell type and quantity in the blood of patients can be conducted through use of different kinds of chips.

In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.

The invention discloses preparation of dendritic molecule-modifiedhydrophilic immunomagnetic beads and application of the hydrophilic immunomagnetic beads to rapid and efficient cell capture. The preparation method comprises the following steps: firstly, synthesizing magnetic nanoparticles through a hydrothermal method; hydrolyzing ethyl orthosilicate and modifying with a silylating reagent in order that the surface of a material is rich in amino groups; coupling dendritic molecules to the surface of the material in order that the material is hydrophilic; lastly, fixing an antibody to the surface of the material to prepare a capture substrate capable of recognizing tumor cells specifically. As proved by experiments, the hydrophilic immunomagnetic beads modified by the antibody and the dendritic molecules can shorten the time of combining the material and cells, perform high-specificity recognition and capture tumor cells rapidly, and capturing efficiency of 86+/-5 percent can be achieved only in 15 minutes; meanwhile, the activity of the cells is kept, and the tumor cells can be successfully separated from whole blood into which a tumor cell standard is added. A template-free, low-cost and low-toxicity material is applied to cell capture, so that the method is novel, convenient, practical and efficient, and has a tremendous clinical application potential.

A cell trapping device includes a housing that includes an inlet opening connected to an inlet line through which a cell dispersion liquid is introduced and an outlet opening connected to an outlet line through which the cell dispersion liquid is discharged; and a filter which is positioned within the housing and includes a trapping region for trapping cancer cells contained in the cell dispersion liquid. The filter is bonded to the housing, at least a part of the trapping region is formed of an observation region for observing the trapping region from the outside, the inlet line and the inlet opening are arranged at outer positions than the observation region when viewed from a normal line direction of the filter, and the inlet line is extended along an in-plane direction of the filter.

The invention belongs to the field of biological fluid detection in vitro, and concretely relates to a urine exfoliated tumor cell micro-fluidic chip detection technology aiming at urothelium carcinoma. The micro-fluidic chip comprises a sample inlet, a cell capturing area and a sample outlet which are connected sequentially, wherein the cell capturing area is provided with a plurality of cell sorting devices; each cell sorting device is formed by three columnar bulges arranged in an arc shape; a gap exists between every two columnar bulges; an arc-shaped opening is used as a liquid flow inlet; gaps at two sides of each middle columnar bulge are used as liquid flow outlets which are symmetrically distributed. According to the urine exfoliated tumor cell micro-fluidic chip detection technology provided by the invention, various cells in urine are separated and captured, multiple downstream cytology staining methods can be compatible for realizing cell recognition, the detection accuracy of urine exfoliated tumor cells in human urine is improved, the dependency on subjective experiences of pathology doctors in a conventional method is avoided, and the whole process is noninvasive. In addition, a lossless cell capturing and recovering manner lays a foundation on downstream molecular biology analysis.

The invention relates to a microfluidic chip for enriched capturing of target cells with different sizes, which is composed of a chip body and a negative film. A chip chamber in the chip body is provided with a sample inlet, an injection isolation column, an enrichment screening area, a capture area, and a non-target cell waste liquid outlet and a target cell waste liquid outlet; a blood sample to-be-treated enters the chip chamber from the sample inlet, and the blood sample is divided into two into the enrichment screening area through the injection isolation column, and the enrichment screening area sequentially enriches and screens the cell mass of the target cell with a diameter larger than the critical separation diameter, cell cluster and single cells, the target cell liquid is obtained, and the remaining blood cell liquid is discharged out of the chip chamber from the non-target cell waste liquid outlet; the target cell liquid enters the capture area, the capture area captures the target cell, and the remaining cell liquid is discharged out of the chip chamber from the target cell waste liquid outlet. The microfluidic chip is suitable for practical applications, can achievehigh capture rate, high purity and high throughput, and improves performance of existing microfluidic chips.

The invention discloses a microfluidic chip for cell culture, and a dynamic culture device and method. The microfluidic chip comprises a chip inlet, a chip outlet, a channel and a cell trapping structure, wherein the cell trapping structure comprises a plurality of cell trapping units; the channel comprises a first channel and a second channel which are mutually communicated; the first channel is communicated with the second channel respectively through a first connected region and a second connected region; each cell trapping unit comprises a cell trapping unit inlet and a cell trapping unit outlet; the cell trapping unit inlet is positioned on the side of the first channel; the cell trapping unit outlet is positioned on the side of the second channel; and the cell trapping unit outlet forms the second connected region. The embryo cell in-vitro dynamic culture device comprises the microfluidic chip, and can be used for performing simple and efficient in-vitro culture on the embryo cells.

The invention discloses a method for trapping single cells. The method is implemented by the aid of a plurality of micro-fluidic chips. A plurality of single cell trapping units with different specifications are arranged on each micro-fluidic chip, cell sap with target cells can flow through the micro-fluidic chips, the target cells can be trapped by the different single cell trapping units according to physical size difference, and the target cells which are not trapped by the single cell trapping units with the matched specifications can continue flowing to the next single cell trapping units until the target cells are trapped or flow through all the single cell trapping units. According to the scheme, the method has the advantages that the single cell trapping units with the different specifications are set, the structures and the sizes of the single cell trapping units on the different micro-fluidic chips can be differently set, accordingly, the target cells with the different sizes can be trapped in the mode, and the purpose of trapping the single cells with the diversified sizes in a high-flux manner can be achieved.

The invention relates to a three-dimensional structural cell capture and release chip, which comprises an upper cover plate and a lower substrate. A microflow channel is etched on the back of the upper cover plate. Two ends of the microflow channel of the cover plate are respectively provided with an inlet and an outlet. A layer of chitosan film is arranged on the substrate. The cover plate and the substrate are glued through an adhesive. By the utilization of the structure, cells/particles can be captured and released. A liquid to be measured passes through the three-dimensional structural channel of the micro-fluidic chip to capture cells/particles with specific size by height characteristics of the three-dimensional structure. After capture, height of the internal channel of the three-dimensional chip is increased by a specific method so as to finally release cells/particles. The operational method of cell capture and release is convenient, has fast response, doesn't damage cells and is suitable for capturing and releasing micro-scale cells and particles. The preparation method has a simple process. The prepared three-dimensional structural chip has characteristic of miniaturization and can be widely used in the field of analysis.

The invention discloses a microfluidic chip integrating circulating tumor cell separation and single cell immunoblotting. The microfluidic chip is characterized by comprising a circulating tumor cellsorting unit, a single cell capture unit, a photoactive gel electrophoresis separation unit and an immunoblotting analysis unit, wherein the circulating tumor cell sorting unit is used for separatingand sorting circulating tumor cells; the single cell capture unit is used for capturing single cells of sorted and enriched cells and performing closed lysis on the cells; the photoactive gel electrophoresis separation unit is used for pushing a protein obtained after cell lysis to a gel coating area on a chip; and the immunoblotting analysis unit is used for performing incubation and elution through a specific antibody, performing fluorescent or luminescent group labeling on a target protein molecule and detecting a signal of the target protein molecule. According to the invention, a varietyof functional units of cell sorting, purification, single cell capture, gel electrophoresis, immunoblotting analysis and the like of the circulating tumor cells are integrated on the micro-fluidic chip, so the separation speed, the analysis speed and the automation degree of the circulating tumor cells are greatly improved.

The invention provides a three-dimensional bionic nanometer material for capturing circulating tumor cells and a preparation method thereof.The material is composed of porous graphene, microballoon spheres and a silicon substrate, a three-dimensional array structure is formed through electrostatic adsorption and self-assembly, chemical modification is carried out on the surfaces of the graphene and the microballoon spheres, E-selectin protein molecules and ligands used for cell capturing are modified and fixed, and a bionic structure for enhancing cell recognition is achieved.The material can be integrated in a micro-fluidic chip, recognition and capturing of the chip for the circulating tumor cells is are enhanced, separation efficiency is improved, and the captured cells can be detected and analyzed conveniently; a fixed recognition ligand is changed, the material can also be used for effectively capturing different types of cells, and rare tumor cells can be recognized and captured from peripheral blood samples.

The invention belongs to the technical field of medical apparatus and instruments, and specially relates to the technical field capable of performing damage endothelium repair on a device implantationposition. The invention provides an application of anti-adhesion polypeptide in preparation of an implanted medical instrument. The implanted medical device is loaded with a cell trapping substance and the anti-adhesion polypeptide. A preparation method of the implanted medical instrument includes the following steps: loading the cell trapping substance on a surface of the cell trapping substancethat the implanted medical device plan to load, and loading the anti-adhesion polypeptide into a hole or a groove of the implanted medical device. According to the technical scheme, from the perspective of anti-cell adhesion, a mechanism of combining the anti-adhesion peptide with an adhesion molecule on a cell membrane is adopted, and the adhesion of other types of cells except captured specialcells to the surface of the implanted medical device is blocked, and therefore, the purity of the captured cells can be greatly improved, the efficiency of endothelial repair is improved, thereby achieving the purpose of promoting endothelial neogenesis, inhibiting intimal hyperplasia, and inhibiting an inflammatory reaction.

A circulating type single-cell capturing chip is disclosed. The chip comprises a liquid layer, microsieves and a micropump, wherein the liquid layer comprises a circular liquid path, the liquid path is communicated with outside of the chip through a liquid feeding pipe and a liquid discharging pipe, the liquid feeding pipe and the liquid discharging pipe are respectively provided with a microvalve for isolating the liquid path from the outside, the microsieves are at least used for intercepting single cells in liquid flow flowing through the liquid path, and the micropump is at least used for driving the liquid flow to flow in the liquid path in a one-way and circulating manner. By utilization of the chip, single cells can be effectively captured from the liquid flow containing a small number of cells, the ratio of the number of the microsieves capturing the single cells reaches 90%, and the capturing efficiency is far higher than that in the prior art.