71 results about "Cell trapping" patented technology

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention relates to the technical field of micro-fluidic chips and cell culture and analysis, in particular to an integrated micro-fluidic system and method for single cell capture, stimulation and analysis, and the integrated micro-fluidic system for single cell capture, stimulation and analysis is formed by integrating two micro-fluidic chips of a front part and a rear part, and the front half part is connected with the rear half part through a capillary hose c. Sorting capture, mechanical stimulation and culture analysis of single cells are achieved through the integrated pretreatment module and the capture culture module, accelerated mixing of cell suspension liquid introduced into the pretreatment module is achieved under the action of curve secondary flow through the first micro-fluidic chip located at the pretreatment module, and the single cells are rapidly captured and cultured through the first micro-fluidic chip located at the position of the first micro-fluidic chip. Cells in the cell suspension are sequenced to a certain extent, the next step is facilitated, and sample cells with required sizes are captured and cultured through a second micro-fluidic chip which consists of a plurality of cell capturing units and is positioned on the rear half part for subsequent observation and analysis.

Intracellular delivery of a genetic construct to immune cells including: obtaining a deterministic mechanoporation (DMP) platform that includes a substrate having a surface and a plurality of capture sites, each said capture site having a boundary shape at the surface adapted and configured to support thereon a cell, and each said capture site having a bottom and including a sub-micron-scale projection extending from the bottom toward the surface of the substrate, wherein said projection is adapted and configured to penetrate a cell membrane and / or wall of the cell, and wherein the substrate has a plurality of aspiration vias situated at the bottom of the capture sites; introducing the cells to the surface in a liquid media; capturing the cells within the capture sites by applying a first hydrodynamic force; applying a second hydrodynamic force on the captured cell and locally rupturing the membrane and / or wall of the cell with the projection, introducing the genetic construct into the cells, and releasing the porated cells from the capture sites. Also disclosed are methods of chimeric antigen receptor (CAR) T cell adoptive immunotherapy and T cell receptor (TCR) therapy.

The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.

The invention relates to the technical field of cell manipulation and analysis, and relates to an automatic single cell continuous capturing and screening system and a use method thereof. Comprising a bias liquid storage module, a cell injection module, a micro-fluidic chip, a cell capturing module, a cell screening module and an imaging system, the bias liquid storage module, the cell injection module, the cell screening module and the cell capturing module are connected with the micro-fluidic chip; the bias liquid storage module is used for generating bias flow; the cell injection module is used for providing cells; the micro-fluidic chip is used for capturing and screening cells; the cell capturing module is used for capturing cells; the cell screening module is used for screening cells; and the imaging system is used for acquiring captured cell images and performing visual analysis. Micro-fluidic pipeline double-bias-flow stable cell flow sample introduction is designed, a high-speed electromagnetic valve is matched to realize rapid control of a gas path on cell capturing and screening channel fluid, and a high-speed camera is combined to analyze cell positions in different areas in real time, so that automatic single cell continuous capturing and screening are realized.

This invention belongs to the field of microfluidics, and particularly relates to the use of a pine pollen microrobot combined with a microfluidic chip for cell capture. The pine pollen microrobot is gold-sputtered pine pollen, and the microfluidic chip is injected with the pine pollen microrobot and a conductive cell solution. When an alternating current signal is applied to the microfluidic chip, the fluid surrounding the pine pollen microrobot generates an induced charge electroosmotic vortex, causing cells in the cell solution to move toward the cavity of the pine pollen microrobot, thereby achieving cell capture. Using induced charge electroosmosis (ICEO) technology, this invention enables the efficient capture of blood cells and other micron-sized cell structures. This solves the problem of traditional capture techniques relying on antibody labeling and mechanical capture, reduces operational complexity and capture difficulty, and improves capture success rate and raw material utilization.

This invention provides a probabilistic modeling method and system for characterizing cell capture performance in single-cell immunoblotting (scWB). First, considering the random cell settling process in a polyacrylamide gel microwell array under gravity, a probabilistic distribution model of the number of non-empty microwells is constructed. The evolution of the number of non-empty microwells under different cell loading amounts is analyzed, and the model's calculation results are validated using single-cell capture experimental results. Considering the constraint that trace protein bands in adjacent microwells may overlap, a probabilistic distribution model of the effective number of microwells is established, and a method for calculating the average number of effective microwells is given, revealing the variation of the average number of effective microwells with the spacing between microwell columns under different cell loading amounts. This invention provides a theoretical basis for further optimization of microwell array structures and significantly improves the cell sample utilization rate of scWB technology.

The application relates to a communication device and a cell capture dyeing instrument thereof, which comprises a frame and a placing seat for placing a to-be-communicated member, the placing seat is slidably connected to the frame, the frame is provided with a first driving mechanism capable of driving the placing seat to slide out of or into the frame; the frame is movably provided with a moving plate, the moving plate is provided with a sample outlet butt joint, and the frame is provided with a second driving mechanism capable of driving the moving plate to drive the sample outlet butt joint to be butt-jointed and communicated with a sample inlet of the to-be-communicated member in the frame. The application has the effect of improving butt-joint communication efficiency of the to-be-communicated member.

This invention provides a method and apparatus for splitting single-cell mixed sample sequencing data, relating to the field of biotechnology. The splitting method includes: capturing and sequencing single-cell suspensions using a single-cell platform; then performing reference genome alignment, cell identification, and gene expression level quantification on the sequencing data using Cellranger; splitting the cell data identified in step a into two groups of cell data for different sexes based on SNP locus information from the 1000 Genomes Project; and distinguishing the two groups of cell data from male or female samples based on the proportion of sex-specific genes expressed in the two groups of cell data. This splitting method eliminates the need for additional experimental operations such as protein labeling and genome sequencing, and can provide accurate and reliable data splitting even when individual SNP information is unavailable.

The application discloses a kind of light stimulation, electric stimulation, concentration gradient combined control device and preparation method, including light stimulation light intensity gradient chip layer, cell capture culture chip layer and microelectrode layer;Cell capture culture chip layer is provided with several main sample introduction sections and several capture culture channels;Capture culture channel is provided with several groups of capture culture cavities, and capture culture cavity is provided with several capture structures;Light stimulation light intensity gradient chip layer is provided with several main liquid introduction sections and several light intensity adjustment channels, and each light intensity adjustment channel is provided with light intensity adjustment section;The interdigital electrode region on microelectrode layer corresponds to the position of capture culture cavity.There is also S1-S5 etc.Preparation step.The light stimulation, electric stimulation, concentration gradient combined control device of the application can realize integrated multi-factor combined stimulation based on microfluidic platform, parallel experiment, high integration, multiple units are operated simultaneously, and has the advantages of small pollution risk, low reagent loss etc..

The invention discloses a flexible micro-nano cross-scale integrated metasurface single-cell sensor and a preparation method thereof, and relates to the field of biosensing. The sensor comprises a PDMS flexible substrate layer, a micro-nano cross-scale structure layer and a gold metal film layer, the micro-nano cross-scale structure layer adopts a nesting design of a micron-scale single cell capture unit and a nano-scale plasmon structure, so that single cell precise limiting and local electromagnetic field multi-stage enhancement are realized, and the problems of poor adaptability and low sensitivity of a traditional rigid sensor are solved. According to the preparation method, a silicon-based template-replication forming-magnetron sputtering process is adopted, a silicon template is prepared through deep ultraviolet lithography and deep reactive ion etching, a gold film is deposited after a flexible micro-nano structure is copied by PDMS, and large-scale mass production of the sensor is realized. The sensor has excellent flexibility, biocompatibility and high detection sensitivity, can realize single cell level accurate and in-situ detection, is suitable for the scenes of early disease screening, cell biological analysis, personalized medical treatment and the like, and is simple in process and low in cost.

Embodiments of the microfluidic device may include of an array of microfluidic cell capture chambers, each functionalized with a different antibody to recognize a target antigen, and a network of code-multiplexed Coulter counters placed at strategic nodes across the device to quantify the fraction of cell population captured in each microfluidic chamber. For example, an apparatus may comprise a fluid inlet port divided into a plurality of separate microfluidic paths, each separate microfluidic path configured to transport a plurality of cells, the plurality of separate microfluidic paths, each comprising a plurality of microfluidic cell capture chambers, an outlet port to discharge a merged output of cells from the plurality of microfluidic cell capture chambers, and a plurality of sensors to detect cells passing into or out of a microfluidic cell capture chamber.

Provided in the present invention is a method for modifying a solid-phase support. The solid-phase support is suitable for adhering to cells. The method comprises: bringing a solid-phase support into contact with a polylysine–protein factor mixed solution, wherein the polylysine–protein factor mixed solution comprises polylysine and a protein factor, and the protein factor comprises at least one of an extracellular matrix protein and a cellular integrin. In the method, by modifying the solid-phase support, a large number of cells can be captured by the solid-phase support, and the cells captured by the solid-phase support have clear outlines, such that the cell capture efficiency of the solid-phase support is improved.

The invention provides a single cell sorting system and sorting method based on laser-induced jet flow, and belongs to the technical field of biomedical engineering and precise instruments, the single cell sorting system comprises a laser-induced module, a jet flow microfluidic sorting chip and a microscopic imaging module; capturing through cells; target identification and jet flow triggering; collecting single cells; non-contact sorting design is achieved, laser only acts on a special coating material, cells are prevented from being directly irradiated, and phototoxicity is reduced; jet flow impact time lt; the cell stress is uniform, the membrane damage risk is reduced, a multi-parameter sorting strategy is adopted: in combination with multi-dimensional information such as morphology and fluorescence, unmarked rare cells or cells in a specific metabolic state can be screened, a lossless sorting effect and a whole-process closed loop are achieved, and the sorted cells can be directly used for culture or downstream single cell sequencing. The method is combined with an in-situ analysis technology, and integration of sorting and analysis is realized.

This invention relates to the field of biomedical engineering, specifically to a device and method for high-throughput continuous sampling and gene detection of single live cells. The device includes a cell capture layer and a gene detection layer stacked sequentially from top to bottom. The cell capture layer includes a fluid inlet and a fluid outlet, and at least one capture array disposed between the fluid inlet and the fluid outlet. The at least one capture array is connected to the fluid inlet and the fluid outlet respectively via a microfluidic network. Each row of the capture array includes multiple capture units spaced apart and connected along the fluid flow direction. Each capture unit includes a main channel extending along the fluid flow direction and branch channels, the branch channels sharing the same fluid inlet and the same fluid outlet with the main channel, thereby forming a θ-shaped capture unit. The gene detection layer has multiple collection chambers spaced apart, each containing an electrode. A nanomembrane is disposed at the top of each collection chamber, and a gene detection probe is disposed at the bottom.

The invention discloses a PDMS (polydimethylsiloxane) micro-fluidic chip for long-term culture of living cells and a preparation method thereof, and relates to the technical field of micro-fluidic cell culture.The PDMS micro-fluidic chip comprises a PDMS main body, and at least one culture flow channel is arranged in the PDMS main body; the culture flow channel is provided with a fluid inlet and a fluid outlet; according to the PDMS micro-fluidic chip for long-term culture of living cells and the preparation method of the PDMS micro-fluidic chip, the mechanical microenvironment of an in-vivo tissue vasculature is effectively simulated, and meanwhile, the design of an open pool with a one-way valve at the tail end is combined, so that automatic removal of bubbles, stable maintenance of system pressure and low-damage convenient recovery of cell samples are realized; therefore, a plurality of key steps of cell capture, long-term culture, sample acquisition and the like are integrated into a simplified and reliable operation process, and the defects of complicated operation, high dependency on auxiliary equipment and insufficient microenvironment uniformity are overcome.

The invention relates to the technical field of microfluidics, and particularly discloses a cell communication micro-fluidic chip with a cell capturing structure and application of the cell communication micro-fluidic chip. The chip comprises main channels, and the two main channels are arranged in parallel at an interval; the cell interception structures are arranged in the main channels, grooves capable of accommodating at least one cell to be cultured are formed in the incident flow surfaces in the main channels, and at least one opening only allowing a culture solution to flow out is formed in each groove, so that the cells can stably stay and are uniformly distributed, and meanwhile, fluid is allowed to pass through; the culture solution interaction structure is arranged between the two main channels and used for interaction of culture solutions in the two main channels, transverse exchange of the culture solutions and cell secretions is achieved, cell migration is prevented at the same time, and therefore the non-contact cell communication model is constructed. The cell communication micro-fluidic chip can efficiently simulate the cell paracrine communication process, and is suitable for various cell communication research scenes such as inflammatory response, immune regulation, tumor microenvironment, stem cell secretion factor effect and the like.

The invention provides a single cell capture chip and system and a use method of the system.The system comprises a liquid inlet and outlet device, a detection device and a control device, and the control device controls the liquid inlet and outlet device according to the capture rate; the method specifically comprises the following steps: a control device controls a piston to move towards a first direction so as to enable cell sap in a first liquid storage tank to flow to an outlet end through a capture well area of a single cell capture chip; then, the steps S1 and S2 are executed circularly until the cell capture rate reaches a preset capture rate; the method comprises the following steps: S1, after standing, detecting the capture rate of cells by a detection device; if the preset capture rate is not reached, controlling the piston to move towards a second direction opposite to the first direction so as to push the cell sap in the capture trap area to move towards the inlet end; s2, after standing, detecting the capture rate of the cells by a detection device; and if the preset capture rate is not reached, the piston is controlled again to move towards the first direction. According to the scheme, the cell capture rate can be increased, and the capture rate of complex cell samples can also be increased.

This application relates to the field of 3D printing technology, specifically to a photomagnetic dual-drive cell capture device and a method for manufacturing the cell capture device. The cell capture device is used to capture target cells from a target sample liquid. The cell capture device includes: a capture clamp composed of multiple claw arms for capturing target cells; each claw arm is made of a magnetic composite material with predictable thermal deformation; a substrate, which is cylindrical and has a cross-sectional end face for fixing the capture clamp; the multiple claw arms are respectively connected to the cross-sectional end face of the substrate; the cross-sectional end face of the substrate is perpendicular to the axial direction of the substrate. The cell capture device provided in this application solves the problems of existing cell capture devices, such as limited functionality, narrow applicability, and easy damage to target cells, thus improving the success rate of cell capture and ease of use.

Provided is a novel cell capture method.　The present technology provides a bioparticle capture method, etc., in which at least a capture step, for capturing a bioparticle having a lipid membrane using a lipid-molecule-modified nucleic acid having a branched structure and a plurality of modified lipid molecules, is performed, and the distance from a branch point of the branched structure to a hydroxyl group that binds a lipid molecule is 3 Å or more.

The invention relates to the field of molecular biological technology high-throughput single cell sequencing, and discloses a non-instrument-dependent high-throughput single cell library building method which comprises the following steps: S1, water-in-oil microdroplet generation; s2, temperature-controlled cell lysis: heating the water-in-oil system to activate the lysis reagent for cell lysis; s3, demulsification and microsphere cleaning: adding a demulsification buffer solution and perfluorooctanol for demulsification, and cleaning to remove invalid mRNA; s4, molecular tag release and reverse transcription; s5, amplifying and purifying the cDNA; and S6, library construction and sequencing. According to the method for generating the water-in-oil droplets without depending on microfluidic equipment, the water-in-oil droplets are generated, about 1-10% of effective droplets contain cells, molecular bar code gel beads and a cell lysis system at the same time, the polycellular rate in the effective droplets is lower than 5%, the cell capture rate is about 45%, and the method can replace the method for generating the water-in-oil droplets through the microfluidic equipment.

The application relates to the field of microfluidic chips, cell culture and analysis, in particular to an integrated microfluidic system and method for single cell capture, stimulation and analysis, wherein the integrated microfluidic system for single cell capture, stimulation and analysis is composed of two microfluidic chips in front and back parts, and the front half part and the rear half part are connected through a capillary hose c. The application realizes sorting capture, mechanical stimulation and culture analysis of single cells through an integrated pretreatment module and a capture culture module. The first microfluidic chip located at the pretreatment module makes the cell suspension flowing into the pretreatment module realize accelerated mixing under the action of the secondary flow of the bend, and the cells in the cell suspension are sorted to facilitate the next step. The second microfluidic chip located at the rear half part and composed of multiple cell capture units captures and cultures sample cells with a required size for subsequent observation and analysis.

The technical idea of the present invention provides a genetic material extraction apparatus comprising: a substrate for accommodating, on a main surface thereof, a sample in which cells are suspended; a first electrode disposed on the main surface of the substrate; a second electrode disposed to face the first electrode along a first horizontal direction, and configured to generate an electric field across the first electrode and the second electrode; a first insulating film disposed on an upper surface of the first electrode and having a first window exposing a portion of the first electrode; a second insulating film disposed on an upper surface of the second electrode and having a second window exposing a portion of the upper surface of the second electrode; and a power source for applying an alternating current voltage to the first electrode and the second electrode so as to generate the electric field for trapping the cells within the first window or the second window.

Disclosed herein include systems, methods, compositions, and kits for the generation and use of oligonucleotide-conjugated beads wherein an individual bead has a different cell label for each capture sequence. The disclosed compositions and methods enable libraries generated from each capture sequence of an oligonucleotide-conjugated bead to be identified separately bioinformatically in some embodiments.

The application discloses a kind of integrated microfluidic biological sensing chip based on micro column array D type optical fiber, belong to biological detection field.This integrated microfluidic biological sensing chip based on micro column array D type optical fiber includes fiber base micro column array D type sensing optical fiber;Periodic micro column array is arranged on the side edge polishing surface of fiber base micro column array D type sensing optical fiber;Micro column array surface is successively coated with sensitizing layer and specific modification layer;The raw material of sensitizing layer includes sensitizing material, and sensitizing material includes MXene material and / or metal nano material, and the raw material of specific modification layer includes biological aptamer material.Micro column array structure can be used as cell capture enhancement interface, forms a large number of capture sites, increases the contact area of cell and chip surface, improves cell capture efficiency.Sensitizing layer can greatly enhance the evanescent field intensity of optical fiber, and biological aptamer material realizes specific identification, and also has good sensitivity and detection accuracy to very low concentration circulating tumor cell.

The invention provides application of polyethylene glycol in reducing diffusion of cell contents in a single-cell suspension and improving single-cell capture efficiency. When the single-cell suspension containing polyethylene glycol is used for capturing single-cell nucleic acid, diffusion of contents of single cells, such as nucleic acid such as genome DNA or transcriptome RNA, can be effectively reduced, so that the contents of the single cells are more gathered, and the cell flux and capturing efficiency are effectively improved.

A droplet array integrated circuit (IC) for biological cell analysis can include a semiconductor IC substrate with a hydrophobic region disposed on a first surface thereof. An array of hydrophilic regions can be positioned on the first surface, with individual hydrophilic regions bordered by the hydrophobic region. The IC can include a plurality of biological cell-adhering elements, with individual elements disposed atop corresponding hydrophilic regions of the array. The biological cell-adhering elements can be selective for adhering to specified cell types, sizes, shapes, or morphologies, enabling precise control over cell capture and positioning.

The invention discloses a phage multifunctional affinity interface as well as a preparation method and application thereof, and belongs to the field of functional biological materials. The preparation method of the phage multifunctional affinity interface comprises the following steps: modifying a phenylboronic acid group by using pVIII protein on the side wall of an M13 phage as a bracket; connecting histidine on the protein of the M13 bacteriophage pIII to the nickel magnetic microbead; the method comprises the following steps: carrying out fluorescence labeling on a biotinylated aptamer targeting EpCAM positive cells, hybridizing with a complementary chain of a labeled fluorescence quencher, and coupling with avidin according to a molar ratio of 4: 1 to obtain an avidin-biotinylated DNA switch; and then the M13 bacteriophage-nickel magnetic microbead containing the phenylboronic acid group is coupled with an avidin-biotinylated DNA (Deoxyribose Nucleic Acid) switch. The capture efficiency of the multifunctional affinity interface to MCF-7 cells reaches 94.2%, the release efficiency reaches 92.7%, and the multifunctional affinity interface has reproducibility and can be used for multiple rounds of cell separation.

The invention relates to the technical field of medical examination, and discloses a cell capturing device suitable for medical examination, which comprises a supporting plate, the top of the supporting plate is fixedly connected with a group of supporting columns, a rotating column penetrates through the supporting columns, the top of a rotating disc is fixedly connected with a connecting plate, and the top of the connecting plate is fixedly connected with a capturing box. A sealing plate is rotationally connected to the capturing box, a plurality of capturing assemblies are arranged in the limiting plate, and a flow guide assembly penetrates through the limiting plate; the capturing assembly is used for capturing the cells entering the capturing box; the flow guide assembly can classify the cells according to different flow speeds and is sealed through the sealing plate, so that leakage of the cells in the capturing process is prevented, the multiple capturing assemblies are arranged in the capturing box, the flow guide assembly penetrates through the limiting plate, the function of adjusting the flow speed is achieved, and the cells can be classified according to needs. Compared with the prior art, the device can adapt to cells with different flow velocities, and the applicability and the flexibility of the device are improved.

This invention discloses a single-cell capture microfluidic chip device, its fabrication method, and related components. The single-cell capture microfluidic chip device includes a glass substrate and a silicon-based cap. Grooves are formed in the glass substrate to create a main channel and side channels. Inlet and outlet are provided in the grooves. The depth of the grooves, the width of the main channel, and the width of the side channels are all greater than the diameter of the cell. The main channel and side channels are respectively located between the inlet and outlet. The main channel is a straight channel, while the side channels are non-linear channels. A capture unit protrudes from the main channel for capturing cells. The silicon-based cap is fixedly connected to the glass substrate at the opening end of the groove, and a sensing window is formed on the silicon-based cap, located above the main channel and side channels. This invention can isolate a vacuum environment, maintain cell viability, and facilitate observation and detection.