14 results about "Cell trapping" patented technology

This invention provides a method and apparatus for splitting single-cell mixed sample sequencing data, relating to the field of biotechnology. The splitting method includes: capturing and sequencing single-cell suspensions using a single-cell platform; then performing reference genome alignment, cell identification, and gene expression level quantification on the sequencing data using Cellranger; splitting the cell data identified in step a into two groups of cell data for different sexes based on SNP locus information from the 1000 Genomes Project; and distinguishing the two groups of cell data from male or female samples based on the proportion of sex-specific genes expressed in the two groups of cell data. This splitting method eliminates the need for additional experimental operations such as protein labeling and genome sequencing, and can provide accurate and reliable data splitting even when individual SNP information is unavailable.

This invention relates to the field of biomedical engineering, specifically to a device and method for high-throughput continuous sampling and gene detection of single live cells. The device includes a cell capture layer and a gene detection layer stacked sequentially from top to bottom. The cell capture layer includes a fluid inlet and a fluid outlet, and at least one capture array disposed between the fluid inlet and the fluid outlet. The at least one capture array is connected to the fluid inlet and the fluid outlet respectively via a microfluidic network. Each row of the capture array includes multiple capture units spaced apart and connected along the fluid flow direction. Each capture unit includes a main channel extending along the fluid flow direction and branch channels, the branch channels sharing the same fluid inlet and the same fluid outlet with the main channel, thereby forming a θ-shaped capture unit. The gene detection layer has multiple collection chambers spaced apart, each containing an electrode. A nanomembrane is disposed at the top of each collection chamber, and a gene detection probe is disposed at the bottom.

This application relates to the field of 3D printing technology, specifically to a photomagnetic dual-drive cell capture device and a method for manufacturing the cell capture device. The cell capture device is used to capture target cells from a target sample liquid. The cell capture device includes: a capture clamp composed of multiple claw arms for capturing target cells; each claw arm is made of a magnetic composite material with predictable thermal deformation; a substrate, which is cylindrical and has a cross-sectional end face for fixing the capture clamp; the multiple claw arms are respectively connected to the cross-sectional end face of the substrate; the cross-sectional end face of the substrate is perpendicular to the axial direction of the substrate. The cell capture device provided in this application solves the problems of existing cell capture devices, such as limited functionality, narrow applicability, and easy damage to target cells, thus improving the success rate of cell capture and ease of use.

The technical idea of the present invention provides a genetic material extraction apparatus comprising: a substrate for accommodating, on a main surface thereof, a sample in which cells are suspended; a first electrode disposed on the main surface of the substrate; a second electrode disposed to face the first electrode along a first horizontal direction, and configured to generate an electric field across the first electrode and the second electrode; a first insulating film disposed on an upper surface of the first electrode and having a first window exposing a portion of the first electrode; a second insulating film disposed on an upper surface of the second electrode and having a second window exposing a portion of the upper surface of the second electrode; and a power source for applying an alternating current voltage to the first electrode and the second electrode so as to generate the electric field for trapping the cells within the first window or the second window.

This invention discloses a single-cell capture microfluidic chip device, its fabrication method, and related components. The single-cell capture microfluidic chip device includes a glass substrate and a silicon-based cap. Grooves are formed in the glass substrate to create a main channel and side channels. Inlet and outlet are provided in the grooves. The depth of the grooves, the width of the main channel, and the width of the side channels are all greater than the diameter of the cell. The main channel and side channels are respectively located between the inlet and outlet. The main channel is a straight channel, while the side channels are non-linear channels. A capture unit protrudes from the main channel for capturing cells. The silicon-based cap is fixedly connected to the glass substrate at the opening end of the groove, and a sensing window is formed on the silicon-based cap, located above the main channel and side channels. This invention can isolate a vacuum environment, maintain cell viability, and facilitate observation and detection.

The application provides a method for modifying a solid support suitable for adhering cells, comprising: contacting the solid support with a polylysine-protein factor mixture, the polylysine-protein factor mixture comprising polylysine and a protein factor, the protein factor comprising at least one of an extracellular matrix protein and a cell integrin. The method modifies the solid support, allows the solid support to capture a large number of cells, makes the profile of the cells captured by the solid support clear, and improves the cell capture efficiency of the solid support.

The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and / or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.

The present invention relates to the technical field of biology, and provides a method for enabling a plasma cell to capture an antibody secreted by the plasma cell. Specifically, the method comprises: labeling the plasma cell with a capture reagent; and performing single-cell encapsulation treatment on the labeled plasma cell to capture an antibody, wherein the capture reagent has the activity of binding the antibody secreted by the plasma cell, and the plasma cell has the activity of secreting the antibody. By means of the method, the antibody secreted by the plasma cell can be recaptured onto the membrane surface of the plasma cell to form a structure similar to a BCR of a memory B cell, enabling a corresponding antigen protein to specifically recognize and bind to the BCR on the membrane surface of the plasma cell, thereby enabling screening and sequencing of an antigen-specific plasma cell.

This invention relates to a bioreactor for cell culture, the bioreactor comprising: a multilayer microfluidic device including a manifold layer and a permeable flow layer below the manifold layer, wherein: (a) the flow layer includes a hydrophobic upper surface and a lower surface, and the hydrophobic upper surface includes one or more grooves for retaining cells; and (b) the manifold layer is configured to define a flow channel above the hydrophobic upper surface of the flow layer, and the manifold layer includes: (i) a first inlet port; (ii) a first outlet port; (iii) one or more first fluid inlets providing fluid communication between the first inlet port and the flow channel; and (iv) one or more first fluid outlets, the one or more of the first fluid outlets providing fluid communication between the first inlet port and the flow channel. A first fluid outlet provides fluid communication between the flow channel and the first outlet port; wherein the one or more fluid inlets and the one or more fluid outlets are positioned in the manifold layer such that fluid entering the flow channel from the one or more first fluid inlets moves toward the one or more first fluid outlets in a first flow direction across the one or more grooves to minimize damage to cells contained therein; and a cell-capturing composition physically adhered to the one or more grooves on the hydrophobic upper surface, wherein the cell-capturing composition comprises (i) an amphiphilic polymer comprising a hydrophobic component and one or more hydrophilic polymer fragments; and (ii) an antigen-binding portion that specifically binds to target cells.

The application provides a single cell analysis chip combining a carbon-based field effect transistor and microfluidics, comprising a plurality of single cell capture units; wherein the single cell capture unit comprises a sensing unit and a PDMS microfluidic channel unit arranged correspondingly to the sensing unit; the sensing unit is a carbon-based field effect transistor, and the PDMS microfluidic channel unit comprises a circular capture trap chamber vertically and directly above a sensing area of the carbon-based field effect transistor and a narrow channel; a first end of the circular capture trap chamber is connected with a first end of a first main channel, a second end of the circular capture trap chamber is connected with a first end of a second main channel through the narrow channel, and the first main channel and the second main channel are connected through an arc-shaped side channel; after cells are captured through the narrow channel, subsequent cells can flow into the next PDMS microfluidic channel unit through the arc-shaped side channel. The chip can separate and capture single cells, and achieve the purpose of detecting the surface proteins of single cells.

The technical idea of the present invention provides a genetic material detection apparatus comprising: a substrate for accommodating, on a main surface thereof, a sample in which cells are suspended; a first electrode disposed on the main surface of the substrate; a second electrode disposed to face the first electrode; a first insulating film disposed on an upper surface of the first electrode and having a first window exposing a portion of the upper surface of the first electrode; a second insulating film disposed on an upper surface of the second electrode and having a second window exposing a portion of the upper surface of the second electrode; a power source configured to apply an alternating current voltage to the first electrode and the second electrode to generate an electric field for trapping the cells within the first window or the second window, and then to increase a peak voltage of the alternating current voltage to rupture membranes of the cells so as to extract genetic material; and a biosensor including a probe capable of binding to the genetic material.

This invention discloses a temperature gradient cell capture culture chip device and method, comprising a cell capture culture chip, a temperature conduction film, and a temperature gradient chip. The cell capture culture chip is provided with an inlet, an inlet channel, an outlet channel, and an outlet, with the inlet and outlet channels correspondingly connected to cell capture culture chambers. The temperature gradient chip is provided with several heating channels, the interior of which is filled with liquid metal material. Each heating channel includes several semi-annular cavities and several linear cavities. The semi-annular cavities on the heating channels combine to form a circular heating area. This invention enables on-chip cell culture studies under dual-factor stimulation conditions of drug concentration gradient and temperature gradient, supporting efficient localization and long-term stable culture of target cells. It can also synergistically apply dual-factor stimulation conditions of drug concentration gradient and temperature gradient, allowing real-time observation of cell proliferation behavior under these stimulation conditions and subsequent metabolite and genomic analysis.

The present application relates to the technical field of microorganism, and particularly relates to a single-cell microfluidic system for bacteria, which comprises a plurality of microchambers, at least two liquid inlet channels and at least one liquid outlet, the two liquid inlet channels are oppositely arranged on two sides of the microchambers, a stopper is arranged between the microchamber and a liquid inlet channel, the stopper is used for preventing the bacteria from flowing into the corresponding liquid inlet channel from the microchamber, and the height of the microchamber is equivalent to the diameter of a single bacterium to be detected. When the bacteria are cultured, the bacteria are independently distributed in the microchambers one by one, and the situation of multiple bacteria overlapping does not occur, so that single-cell capture and tracking are facilitated, and an ideal platform is provided for tracking single-bacterium division and observing the change of the bacterium morphology under different antibiotic pressures in real time.