116 results about "Sorted Cells" patented technology

The invention provides a flow sorter liquid drop delay adjusting method and system based on real-time cell flow velocity, relates to the field of flow sorting, and ensures that the liquid drop delay time can not be delayed even if factors such as temperature, nozzle state, air pressure and pipelines are changed in the sorting process by monitoring the cell flow velocity in real time and dynamically adjusting the liquid drop delay time. And the charge loading time of the liquid drop can be accurately controlled. Meanwhile, the liquid drop delay time is dynamically adjusted in real time, so that the random distribution of the cell flow velocity during high-speed sample loading can be effectively dealt with, and the cells at different positions can be accurately sorted. The automatic calibration and self-stabilization functions are achieved, a large number of experiment articles and a complex optimization process are not needed, accurate control over liquid drop delay can be completed only through real-time monitoring and adjustment on the basis of liquid drop delay initial value setting, the separation efficiency is improved, the experiment cost and the operation difficulty are reduced, and the separation efficiency is improved. And the cell sorting requirements in various biomedical fields are met.

The invention provides a marker of spinal cord functional neural precursor cells, a detection method and a sorting method, and belongs to the technical field of biological medicine. The invention provides application of a marker detection reagent in preparation of a kit for detecting spinal cord functional neural precursor cells. The invention further provides a sorting-based method for separating the SFNPCs cells, and the cells are sorted through the Marker of the specific SFNPCs, so that the cell purity of the SFNPCs is remarkably improved, the difference between batches is reduced, and a high-purity and homogeneous SFNPCs cell population is obtained. The survival rate of the SFNPCs cells separated and subcultured to the P9 generation by the method disclosed by the invention is more than 85%, and the SFNPCs cells have a good treatment effect on a spinal cord injury model.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The invention discloses a method for screening and identifying ribosome entry sites in a short chain, which comprises the following steps of: (1) screening a short chain IRES sequence with the length of less than 100nt from an IRES database, constructing a screening library and synthesizing; (2) constructing a screening vector containing double reporter genes, cloning the short-chain IRES into a linearized screening vector, and generating circular RNA which only depends on the short-chain IRES sequence to recruit ribosome expression of the reporter genes in cells by the screening vector cloned with the short-chain IRES sequence; and (3) carrying out cell transfection on the screening vector cloned with the short-chain IRES sequence, screening out cells expressing reporter genes through cell sorting, and sequencing through a ribosome-newborn peptide chain compound to obtain the short-chain IRES sequence capable of recruiting ribosome to start circular RNA translation in the cells. According to the invention, a short-chain IRES sequence library is constructed, and short IRES capable of starting circular RNA translation is screened, so that transfection limitation caused by overlarge molecular weight of the current IRES sequence is overcome.

The application provides a preparation method of a multi-layer sandwich type epoxy group modified magnetic microsphere and application thereof. The multi-layer sandwich type epoxy magnetic microsphere prepared by the preparation method provided in the application can realize efficient and accurate cell sorting through coupling of an antibody, is suitable for cell sorting related scenes such as stem cell separation, tumor cell detection, immune cell treatment (such as CAR-T cell preparation) and pathogenic microorganism enrichment, and provides key material support for cell sorting technology in life science research and clinical diagnosis and treatment.

The application relates to the technical field of cell sorting, and specifically provides a feature fusion method and system for high-throughput cell sorting. The method comprises the following steps: acquiring real-time image feature distribution deviation according to real-time image feature distribution and reference image feature distribution, and acquiring real-time spectral feature distribution deviation according to real-time spectral feature distribution and reference spectral feature distribution; calibrating all real-time image features according to the real-time image feature distribution deviation, and calibrating all real-time spectral features according to the real-time spectral feature distribution deviation; for each cell to be sorted, fusing real-time image features and real-time spectral features to obtain a fusion feature vector, and then classifying the cell to be sorted according to the fusion feature vector; the method can effectively improve the long-term stable operation capability, performance repeatability, reliability in standardized application scenarios and reduce the complexity and time consumption of system calibration and troubleshooting of a high-throughput cell sorting system.

The utility model relates to the technical field of magnetic frames, and discloses a cell sorting magnetic frame which comprises a base, the top of the base is fixedly connected with a fixing plate, the top of the fixing plate is provided with a top plate, the top of the top plate is provided with a placing groove, the top of the base is provided with a positioning groove, and the side face of the fixing plate is provided with an installation groove. Mounting grooves are formed in the side faces of the fixing plates, magnetic strips are mounted in the mounting grooves, connecting grooves are formed in the side faces of the fixing plates, mounting plates are mounted in the connecting grooves, and bolts are in threaded connection with the surfaces of the mounting plates. According to the cell sorting magnetic frame, the base and the fixing plate are used in cooperation, meanwhile, the mounting grooves are utilized, so that magnetic strips are conveniently mounted in the mounting grooves, when the magnetic strips are fixed, the mounting plates can be mounted in the connecting grooves, meanwhile, bolts are rotated, the positions of the mounting plates can be fixed, and therefore the magnetic strips can be fixed.

One approach to the exploration method includes calculating a TAD score representing transcriptional activity for each TAD present in the genome and selecting TADs based on the TAD scores. Another approach includes analyzing a genome obtained from cells expressing a foreign gene and selecting the boundaries of regions containing the foreign gene. Cell preparation involves inserting the target gene into the regions identified by the exploration method. Cell sorting involves calculating a TAD score representing transcriptional activity for each TAD present in the genome, selecting TADs based on the TAD scores, and selecting cells containing the target gene in the selected TADs. The cells are derived from Chinese hamsters and are cells in which the target gene has been inserted into at least one specified region. The method for manufacturing the cell product includes culturing the cells to express the target gene.

A cellular mechanical property measurement and sorting method comprises: cells to be sorted being arranged in a straight line after passing through a standing wave acoustic field generated by a first interdigital transducer; the cells flowing through a focused acoustic field generated by a second interdigital transducer, and the focused acoustic field generating a radiation force on the cells, such that the cells are deformed; performing calculation according to information of the radiation force and deformation, so as to obtain elastic moduli of the cells; determining the positions of the cells, and selectively driving, with designed delay time, interdigital transducers in a cell sorting unit according to the moving speeds of the cells and the distances between the cells and the sorting unit, and triggering, according to the elastic moduli, a corresponding interdigital transducer to generate a planar acoustic field, so as to sort the cells.

The present application relates to a kind of nanometer magnetic beads for cell sorting and its preparation method.The preparation method of nanometer magnetic beads of the present application includes the following steps: (1) under ice bath condition, prepare aqueous solution containing trivalent iron and divalent iron;(2) using alkaline substance to adjust the pH value of the aqueous solution containing trivalent iron and divalent iron to 10-12;(3) reaction is obtained under 65-90 DEG C aqueous solution containing the nanometer magnetic beads.The preparation method of the present application can control the median particle size of nanometer magnetic beads in 50-200nm, and good particle size uniformity, while guaranteeing the sorting efficiency of cell, the function of target cell is little, and biocompatibility is good, has good application prospect in the field of biological medicine.In addition, the preparation method of the present application is simple and easy to operate, and is suitable for large-scale industrial production.

The utility model relates to the technical field of cell sorting and detection, in particular to a micro-fluidic chip. The micro-fluidic chip comprises a substrate, a micro-fluidic chip, a micro-fluidic chip and a micro-fluidic chip, wherein a metal film layer is arranged on the substrate; the cover plate is at least provided with a micro-channel; the intermediate bonding layer is located between the substrate and the cover plate and covers the metal film layer; wherein the cover plate is a PDMS (Polydimethylsiloxane) prepolymer cover plate; the intermediate bonding layer is a silicon dioxide layer. According to the utility model, the problem that the PDMS prepolymer cover plate and the metal film layer substrate are difficult to directly bond can be solved.

The application discloses a cell sorting method and system based on an image acoustic flow control cell sorting model and a computer readable storage medium, the image acoustic flow control cell sorting model comprises a cell image recognition module and a cell ejection module, and the cell sorting method comprises the following steps: acquiring an original cell image set and a predetermined sample cell image set; inputting the sample cell image set into the cell image recognition module for feature extraction to obtain image category information of the cell image; inputting the image category information and the sample image set into the image acoustic flow control cell sorting model for training; inputting the original cell image into the trained image acoustic flow control cell sorting model for image prediction to determine a target image corresponding to the image category; and ejecting a cell corresponding to the target image to a preset collection area based on the cell ejection module. In the embodiment of the application, the automatic classification of cells can be performed by using a label-free method, so that the purification and collection of target cells are realized.

The invention relates to a cell sorting system and method based on a micro-fluidic chip and micro-nano control, and relates to the technical field of cell sorting, the system comprises the micro-fluidic chip composed of a top layer, a middle layer and a bottom layer which are attached in sequence; a main channel and a diversion channel are arranged at the boundary of the top layer and the middle layer, the diversion channel is communicated with the side wall of the main channel, and the middle layer is provided with a sampling hole located in the bottom wall of the main channel; a sample channel is arranged at the boundary of the middle layer and the bottom layer; the sample introduction hole only allows single cells to pass through in sequence; under the limitation of the size of the sample injection hole, a sample flow in the sample channel enters the main channel to form a narrow sample flow. When the single cell data acquisition time of the cell detection module is less than the time difference of two adjacent cells flowing through the capture point of the micro-nano control module in the narrow sample flow, high-throughput cell detection can be carried out in the narrow sample flow.

Aspects of the present disclosure include methods for adjusting a particle classification index in response to one or more saturated data signals based on detected light from particles in a fluid stream. Methods according to certain embodiments include detecting light from particles in a fluid stream, generating a plurality of data signals based on the detected light, identifying one or more saturated data signals, generating a saturation signal index corresponding to the identified saturated data signals, and applying the saturation signal index to a particle classification index to generate an adjusted particle classification index. In some embodiments, the methods include determining one or more parameters of a particle (e.g., for a particle sorting decision) by calculating an adjusted spectral unmixing matrix for fluorescence of the particle that excludes the one or more saturated data signals. Systems and integrated circuit devices (e.g., field programmable gate arrays) for implementing the subject methods are also provided. Non-transitory computer readable storage media are also described.

The present invention discloses a magnetically driven portable multi-cell separation device. According to one embodiment of the present invention, the magnetically driven portable multi-cell separation device includes: A microscope capable of nano-scale observation, A chip unit, positioned below the microscope, enabling selective cell sorting and capture, A magnetic field generator, which is adjacent to the chip unit and can control the magnetic field via a magnetic member, and A control unit, which controls the operation of the imaging unit, the chip unit, and the magnetic field generator.

This invention discloses an APLN monoclonal antibody, its preparation method, and its applications. The APLN monoclonal antibody includes a light chain variable region and a heavy chain variable region. The amino acid sequence of the light chain variable region is shown in SEQ ID No:1, and the amino acid sequence of the heavy chain variable region is shown in SEQ ID No:2. The APLN monoclonal antibody is obtained by immunizing mice with APLN protein, followed by single-cell B-cell sorting and single-cell PCR. Utilizing advanced single-cell B-cell cloning technology combined with single-cell PCR, a novel APLN monoclonal antibody has been developed. The obtained antibody sequence is the result of natural affinity maturation screening by the in vivo immune system, requiring no additional humanization or in vitro affinity maturation steps. It retains the natural high affinity and specificity, and can directly obtain all-natural antibodies with high affinity and high specificity.

Described herein are systems and methods for sorting and selecting cells by immobilizing or enclosing cells in degradable hydrogel chambers, including a method of sorting cells comprising: (a) providing a fluidics device comprising: (i) a channel comprising a surface, (ii) a spatial energy modulation element in optical communication with the surface, and (iii) a detector in optical communication with the surface and in operable association with the spatial energy modulating element; (b) loading the channel with cells and one or more polymer precursors so that the cells are disposed on or adjacent to the surface; (c) immobilizing one or more cells selected based on one or more optical signals therefrom by synthesizing one or more gel structures enclosing each of the one or more cells; and (d) removing from the channel unselected cells.

The utility model discloses a cylindrical lithium battery sorting device. Belongs to the technical field of lithium batteries. According to the technical key points, the device comprises a box body, two supports are fixedly connected to the position, close to the right side, of the top of the box body, a sorting plate is fixedly connected between the two supports, a plurality of sorting openings distributed in a linear array are formed in the sorting plate in a penetrating mode, and the areas of the sorting openings are sequentially increased from left to right; a material pushing mechanism is arranged on the sorting plate, the top of the box body is fixedly connected with a conveying pipe through a supporting rod, the conveying pipe is located over the sorting plate, and the conveying pipe is arranged in an inclined mode. According to the cylindrical lithium battery sorting device, the sorting plate, the sorting opening, the pushing mechanism, the conveying pipe, the quantitative discharging mechanism, the vertical pipe and the collecting cavity are used in cooperation, the purpose of automatically sorting cylindrical lithium batteries of different specifications is achieved, and the cylindrical lithium battery sorting device has the advantages of being simple in structure, good in sorting effect, low in manufacturing cost and convenient to maintain.

The utility model provides a magnetic bead sorting NK immune cell preparation and extraction device which comprises a base, a control shell is upwards arranged on the base, a magnetic field shell is arranged in the middle of the front side of the control shell, and a plurality of magnetic field grooves are inwards formed in the front side of the magnetic field shell. Each magnetic field groove comprises a first-level magnetic field groove located on the upper portion and a second-level magnetic field groove located on the lower portion, the section of each first-level magnetic field groove is larger than that of the corresponding second-level magnetic field groove, and a first-level magnetic field electromagnet is arranged on the periphery of each first-level magnetic field groove in the magnetic field shell. A secondary magnetic field electromagnet is arranged on the periphery of each secondary magnetic field groove in the magnetic field shell; the chromatographic column is divided into a sample section, a first-stage chromatographic section matched with the first-stage magnetic field groove and a second-stage chromatographic section matched with the second-stage magnetic field groove from top to bottom. The utility model provides a grading magnetic bead sorting structure, which can effectively avoid the blocking phenomenon, effectively improve the sorting efficiency of cells and improve the final yield.

This specification provides a control method and apparatus for a cell mechanical property measurement and sorting system. Based on this method, the control module first controls a camera to acquire target images of the target cells in a matching manner according to the operating parameters of the first interdigital transducer, obtaining relatively good target images. Then, the control module performs a basic first processing on the target images, with relatively small data processing volume, to quickly obtain the first processing result. Based on the first processing result, the second interdigital transducer is controlled to perform corresponding cell sorting processing. Simultaneously, the target images are sent to a computer device via a PCIe interface, utilizing the computer device's data processing resources to perform a more refined second processing on the target images, with a relatively larger data processing volume, obtaining the second processing result. This effectively reduces system latency and efficiently and accurately controls the corresponding modules in the system to cooperate in completing the cell sorting process.

The utility model discloses a cell sorting kit which comprises a placing frame, a placing groove is formed in the surface of the top of the placing frame, a plurality of groups of placing racks are placed in the placing groove, and a plurality of groups of placing grooves are uniformly formed in the tops of the placing racks. According to the cell sorting kit, by arranging a clamping block, a connecting plate, a first spring and the like, a placing frame can be conveniently taken out of a placing frame, by arranging the placing frame, a supporting frame, a supporting rod and the like, the placing frame can be stably placed on a table top through the supporting frame and the supporting rod, the phenomenon that the placing frame topples over is prevented, and the cell sorting kit is convenient to use. By arranging the placing frame, the limiting block, the second spring and the like, the reagent bottle inserted into the placing groove formed in the placing frame can be fixed, and the placing groove formed in the placing frame is not a placing through hole, so that the phenomenon that the reagent bottle placed in the placing groove formed in the placing frame falls off can be prevented.

This utility model discloses an adjustable lithium battery cell sorting device, comprising: a base plate, and a fixed frame mounted on the top of the base plate. An adjustment component is installed on the inner wall of the fixed frame, and a detection platform is rotatably mounted on the top outer wall of the fixed frame. One top end of the adjustment component is rotatably connected to the bottom of the detection platform. A placement frame is fixed on the top outer wall of the base plate, and a bending plate is fixed on the top outer wall of the base plate. In this adjustable lithium battery cell sorting device, cells can be placed inside the detection platform for internal resistance testing. Two conveyor belts can transport cells with different internal resistances. Based on the test results, the horizontal state of the detection platform can be adjusted by the adjustment component, causing the platform to tilt so that the cells on the platform automatically slide onto the corresponding conveyor belt and are directly transported to the next processing station. This eliminates the need for complex clamping and shifting devices for cell sorting, making it more convenient.

A method for classifying one or more cells comprises receiving data associated with the one or cells, the data including, for each respective cell, information associated with one or more measurable parameters of the respective cell; inputting at least a portion of the data into a machine learning model; and receiving, from the machine learning model, an indication of an identity of at least one of the one or more cells.

The invention discloses a method and a kit for rapidly screening gene editing positive monoclone based on qPCR (quantitative polymerase chain reaction), and belongs to the field of gene engineering. The method comprises the following steps: preparing a monoclonal sample: preparing a single-cell suspension from cells subjected to gene editing, and carrying out single-cell sorting and culture until monoclone is formed; collecting monoclonal cells, and directly cracking by using a cracking solution to prepare a qPCR template; qPCR detection: carrying out qPCR amplification and detection on the sample by using a specific primer and a probe designed aiming at a target gene editing event; and result judgment: judging whether the monoclone is gene editing positive clone or not by analyzing the Ct value of the qPCR. According to the present invention, the specific primers and the probe of the target gene editing site are designed, and the qPCR is combined to directly detect the genome variation of the monoclonal cell so as to achieve the early rapid screening;

The utility model relates to cell sorting technical field discloses a cell sorting device, including frame body, the rotatory setting has the pivot in the frame body, and the pivot installs two symmetrical distribution's rotary frame, and the rotary frame is installed with the placing frame away from the pivot one end, and the placing frame is the U shape, the inner wall of placing frame is provided with a plurality of interval distribution's upper test -tube rack and lower test -tube rack, the utility model discloses a plurality of test -tube that installs mixed solution is placed in the upper test -tube rack and lower test -tube, then through the limiting mechanism to the top of test -tube is limited, avoids the movement of test -tube rack in the upper test -tube rack and lower test -tube rack in the process of mixing, improves the stability in the test -tube mixing process, drives the transmission mechanism through the drive mechanism, and the transmission mechanism will drive the pivot reciprocating rotation, makes the rotary frame drive placing frame swing to and fro left and right, thereby can mix the mixed solution in the test -tube fully, avoids the problem such as the trouble and the hard work caused by artificial manual mixing, and the shaking efficiency is low.

The utility model discloses a movable microwell plate collecting device, and relates to the technical field of cell sorting, the movable microwell plate collecting device comprises a device carrier, a storage unit and a movement unit; the storage unit is used for fixing the microporous plate; the motion unit comprises a motion carrier, a first motion assembly and a second motion assembly; the movement carrier, the first movement assembly, the second movement assembly and the storage unit are matched with one another, so that the movement control of the storage unit in two horizontal axial directions is realized, and the movement control of the microporous plate is further realized; the technical problems that the stability and the precision of liquid drop distribution and the design flexibility are influenced due to movement control of the liquid drop distribution device are effectively solved. And the storage unit is designed to be used for fixing the microporous plate, and meanwhile, a temperature control module mounting space is reserved, so that the temperature control module and the microporous plate are integrated together and are controlled by the moving unit together, and the temperature control requirement is met.

The invention relates to the technical field of biomedical treatment, and discloses a cell sorting and culturing method and device, computer equipment and a storage medium. The cell sorting and culturing method comprises the following steps: separating a sample solution by using a density gradient centrifugation method to obtain a to-be-cultured cell solution; conveying the to-be-cultured cell sap into culture equipment through first pipeline switching operation, and performing culture treatment on the to-be-cultured cell sap to obtain cultured cell sap; and obtaining a culture cell sap through a second switching pipeline operation, and concentrating and cleaning the culture cell sap to obtain a target cell sap. According to the invention, a totally-enclosed one-stop sterile operation environment can be ensured, the pollution risk during cell operation site change is avoided, and the cell treatment stability is improved. Meanwhile, continuous operation between sorting and culture treatment of the cell sap can be achieved through two times of pipeline switching operation, the automation degree and continuity of cell treatment are improved, the operation time is saved, and the cell treatment efficiency is improved.

This application discloses a communication method and apparatus, relating to the field of communication technology, for reducing access latency of RedCap terminals. The method includes: obtaining a first cell list; determining the cell order of the first cell list based on cell information; and performing cell search or cell reselection based on the cell order. The first cell list is either a cell search list or a cell reselection list, and the cell information includes at least one of energy, signal-to-noise ratio (SNR), RedCap capability, bandwidth, or cell type. Compared to simply sorting cells in the first cell list based on energy and SNR, this solution additionally considers RedCap capability, bandwidth, and cell type during the cell sorting process. The RedCap terminal can use RedCap capability, bandwidth, and cell type to prioritize cells in the first cell list that are eligible for access, thereby enabling it to use cells at the top of the first cell list to complete access during cell search or reselection, thus reducing cell access latency.

The invention relates to a cell capacity grading and matching all-in-one machine based on pairing index dynamic planning, and the all-in-one machine comprises an automatic feeding and transmission system which is used for carrying out the automatic feeding, code scanning recognition and station sequence circulation of retired power battery cells; the high-precision capacity grading test module is used for acquiring the n-dimensional performance parameters of the battery cell; and the pairing index calculation module is used for calculating a pairing index between any two battery cells according to a formula P (i, j) = 1 / (1 + sigma {k = 1... n} wk.x {ik}-x {jk} / sigma k) on the basis of n-dimensional performance parameters measured from the battery cells. According to the invention, high-efficiency and high-consistency production of the battery module is realized through a highly collaborative automatic and intelligent process, after specification parameters of the battery cell are input, the system automatically adjusts the clamp, the conveying line and the code scanner, then the battery cell is bound with a unique ID through automatic feeding and code scanning, and enters a high-precision capacity grading test link; on the basis of the key performance data of each battery cell, the edge calculation unit can quickly calculate a pairing index matrix among all the battery cells.

The invention discloses a method and a system for position detection and real-time calibration of cells in liquid drops of a flow cytometer. According to the method, one-to-one correspondence of cells is ensured by adopting delayed stroboscopic detection, real-time mapping of droplet detection-breakpoint time difference-cell position is realized through sliding window dynamic modeling, and positioning deviation caused by fluid fluctuation, environmental interference and device attenuation is compensated in the whole process by combining abnormal sample removal and model precision self-checking, so that the positioning accuracy of the cells is improved. And the droplet delay calibration is synchronously completed. The vertical position of the cell in the liquid drop can be directly judged, the positioning precision is 5-10 microns, the cell corresponding accuracy rate is larger than or equal to 99%, the sorting purity is improved to 99% or above, the problems that in the traditional technology, only liquid drop delay is calculated, real-time calibration cannot be achieved and the like are solved, and the method is suitable for a high-precision cell sorting scene.