315 results about "Sorted Cells" patented technology

The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w / 3 to about ¾w, and wherein the flow cell has a height of between about 1 / 12w to about ⅛w.

The present invention relates to a microfluidic multiplexed immunoassay system based on flow magnet-activated cell sorting, and a method thereof. The system comprises a multiplexed immunoassay magnetic bead preparation module, a flow magnet-activated cell sorting microfluidic chip system, an optical detection module, and an image processing module, and achieves ultra-sensitive and rapid multiplexed protein quantitative detection by means of magnetic bead coding based on different magnetic contents and microfluidic chip decoding based on flow magnet-activated cell sorting. The microfluidic multiplexed immunoassay system of the present invention has the advantages of high detection sensitivity, short detection time, high signal-to-noise ratio, convenient and simple operation and low cost, is particularly suitable for multiplexed ultra-sensitive detection for monomolecular proteins, and has a potential economic value.

The invention relates to the technical field of cell sorting, in particular to a sorting control system and a sorting method of a flow cytometer, the system comprises a data acquisition unit and a control unit, and the method comprises the following steps: acquiring a plurality of breakpoint images of liquid drops to determine whether the forming process of the liquid drops is qualified or not according to the pixel variance of breakpoints; determining whether the oscillation stability is qualified or not according to the oscillation area of the oscillation curve of the piezoelectric ceramic based on the unqualified droplet forming process so as to determine whether the sheath fluid flow velocity is obtained or not; determining a matching degree based on the flow velocity curve of the sheath fluid flow velocity and the frequency curve of the liquid drop to increase the sheath fluid pressure according to the fluctuation intensity of the fluorescence signal; determining a target liquid drop according to a fluorescence detection result, determining liquid drop delay according to the target liquid drop, and determining whether the target liquid drop can fall into the collecting pipe or not according to the initial deviation angle of the target liquid drop, so as to adjust the inter-plate voltage of the deflection plate. The sorting precision is improved.

The utility model relates to the technical field of cell sorting, and discloses a fluorescence signal enhancement device which comprises a pre-magnetization assembly and a magnet assembly, the pre-magnetization assembly is used for generating a pre-magnetization magnetic field, and magnetic particles in liquid drops are magnetized and agglomerated in the pre-magnetization magnetic field; the magnet assembly is used for generating a first magnetic field, and the magnetic particles agglomerated in the liquid drops are pulled into a linear shape in the first magnetic field. The utility model further discloses a fluorescence signal detection device and a liquid drop observation device.

The invention discloses an inertial micro-fluidic chip integrating cell focusing and sorting and a preparation method thereof. The inertial micro-fluidic chip comprises a cover plate layer, a runner layer and a substrate layer, a chip inlet, a first chip outlet and a second chip outlet are formed in the cover plate layer; the flow channel layer is provided with a sample liquid flow channel inlet, a first flow channel outlet and a second flow channel outlet, the chip inlet is communicated with the sample liquid flow channel inlet, the first chip outlet is communicated with the first flow channel outlet, and the second chip outlet is communicated with the second flow channel outlet. The flow channel layer is provided with a spiral cell sorting flow channel and a cell focusing flow channel, the front end of the spiral cell sorting flow channel is communicated with a sample liquid flow channel inlet, the tail end of the spiral cell sorting flow channel is communicated with the cell focusing flow channel and a first flow channel outlet, and the tail end of the cell focusing flow channel is communicated with a second flow channel outlet. Through a microfluid vortex focusing technology and a spiral flow channel inertia sorting technology, accurate sorting and focusing of cells are realized, and the problems that the focusing state of the sorted cells is influenced and the cell counting detection precision is low are effectively solved.

Disclosed is a computer-implemented method for real-time classification of cells in a body tissue that includes capturing one or more images of a cytology slide sample of the body tissue, pre-processing the captured one or more images, detecting and extracting a plurality of regions of interest in the pre-processed images using a cell detection process, wherein each region of interest includes one or more cells or cell clusters of interest, segmenting each individual cell or cluster in the regions of interest and extracting pixel content of each segmented cell or cluster, extracting semantic features from each segmented cell image using an embedding extraction model, classifying each individual cell or cell cluster based on corresponding extracted sematic features, and predicting a class of the cytology slide sample, based on one or more positions and relationships among one or more classified cells, using a slide level decision model.

The invention relates to the field of cell analysis, and provides a multi-mode digital micro-fluidic system and method for cell sorting analysis, the system comprises an active digital micro-fluidic platform, the active digital micro-fluidic platform comprises a micro-fluidic chip, an upper computer control system and an FPGA hardware circuit, an upper computer generates a liquid drop path planning instruction, the FPGA hardware circuit converts the liquid drop path planning instruction into an electrode driving signal, and the electrode driving signal is transmitted to the micro-fluidic chip; the nanoliter-grade liquid drops are driven to change between the electrodes; the optical tweezers platform comprises a laser device, an optical microscope and a CMOS camera, laser beams are generated by the laser device to form a three-dimensional optical potential well, the laser beams are transmitted into the micro-fluidic chip through the optical microscope to realize non-contact control on cells, and the CMOS camera records images and transmits the images back to the upper computer; the objective table clamp is used for realizing cooperative control of the optical tweezers and the active digital microfluidics; and the image recognition system is used for recognizing morphological characteristics of cells in the liquid drops based on a convolutional neural network and screening target cells. According to the invention, integration of high-throughput nondestructive cell sorting and in-situ multi-modal analysis is realized.

The present invention provides a real-time flow cytometric analyzer of single-cell dynamics combining droplet microfluidics and multimodal optical microscopy, and belongs to the field of cell biology experimental devices. The analyzer comprises a cell / signal loading & acquisition and control system, a high-throughput microfluidic single-cell manipulation chip, a multi-channel optical microscopy and multimodal signal acquisition and control system, and a data processing and analysis system. Based on droplet-based microfluidic chip technology, multimodal optical microscopy technology, single-cell dynamics theoretical modeling and analysis methods, and artificial intelligence technology, the integrated detection of spatiotemporal information of single-cell dynamics can be realized. The analyzer provides a new rapid and accurate single-cell dynamics analysis platform for exploring the occurrence and development mechanism of major human diseases, disease diagnosis, cell sorting and drug screening, etc.

The invention discloses an NKT cell culture optimization method, and belongs to the technical field of cell culture.The method comprises the steps that firstly, PBMC is sorted through a spiral micro-fluidic chip integrated with CD3 / CD56 double-labeled magnetic beads, and CD3 + CD56 + cells with the purity larger than or equal to 95% are obtained through the synergistic effect of dielectrophoretic force and a magnetic field; then, in a GIBCO culture solution added with the IL-2 / IL-15 / alpha-GalCer sustained release microspheres, the pH, dissolved oxygen and lactic acid concentration are accurately regulated and controlled through a real-time monitoring system, and temperature-oxygen partial pressure gradient culture is adopted; and finally, three times of centrifugation are combined with NKT4 solution treatment, and the cells are collected in cooperation with a gradient cooling procedure, so that the cell activity maintenance rate is greater than or equal to 95%. Compared with the traditional technology, the method has the advantages that the NKT cell sorting purity and activity are remarkably improved, the culture environment is regulated and optimized through dynamic metabolism, and accurate control over the culture process is achieved.

The present disclosure relates to automated systems and methods adapted to quickly and accurately train a neural network to detect and / or classify cells and / or nuclei. The present disclosure also relates to automated systems and methods for using a trained cell detection and classification engine, such as one including a neural network, to classify cells within an unlabeled image.

The invention provides a flow sorter liquid drop delay adjusting method and system based on real-time cell flow velocity, relates to the field of flow sorting, and ensures that the liquid drop delay time can not be delayed even if factors such as temperature, nozzle state, air pressure and pipelines are changed in the sorting process by monitoring the cell flow velocity in real time and dynamically adjusting the liquid drop delay time. And the charge loading time of the liquid drop can be accurately controlled. Meanwhile, the liquid drop delay time is dynamically adjusted in real time, so that the random distribution of the cell flow velocity during high-speed sample loading can be effectively dealt with, and the cells at different positions can be accurately sorted. The automatic calibration and self-stabilization functions are achieved, a large number of experiment articles and a complex optimization process are not needed, accurate control over liquid drop delay can be completed only through real-time monitoring and adjustment on the basis of liquid drop delay initial value setting, the separation efficiency is improved, the experiment cost and the operation difficulty are reduced, and the separation efficiency is improved. And the cell sorting requirements in various biomedical fields are met.

The invention provides a marker of spinal cord functional neural precursor cells, a detection method and a sorting method, and belongs to the technical field of biological medicine. The invention provides application of a marker detection reagent in preparation of a kit for detecting spinal cord functional neural precursor cells. The invention further provides a sorting-based method for separating the SFNPCs cells, and the cells are sorted through the Marker of the specific SFNPCs, so that the cell purity of the SFNPCs is remarkably improved, the difference between batches is reduced, and a high-purity and homogeneous SFNPCs cell population is obtained. The survival rate of the SFNPCs cells separated and subcultured to the P9 generation by the method disclosed by the invention is more than 85%, and the SFNPCs cells have a good treatment effect on a spinal cord injury model.

The embodiment of the invention discloses a magnetic separation chip for cell separation and a preparation method thereof.The magnetic separation chip structurally comprises a sample layer, a functional layer, a clamp set and a magnetic field source, the sample layer is composed of a flow channel layer and a sealing film, and the flow channel layer is provided with a sheath fluid inlet, a sample inlet and a sample outlet; the sealing film is attached to the open surface of the runner layer to seal the runner; the functional layer is located below the sample layer and comprises a patterned substrate and an amorphous alloy soft magnetic strip material, the patterned substrate is provided with a counter bore groove, the amorphous alloy soft magnetic strip material is embedded into the counter bore groove, and the upper surface of the amorphous alloy soft magnetic strip material is flush with the upper surface of the substrate, right faces the sealing film and is arranged in the extending direction of the flow channel; the clamp group comprises an upper clamping plate and a lower clamping plate, the upper clamping plate is arranged above the sample layer, the lower clamping plate is arranged below the functional layer, the upper clamping plate is provided with runner holes corresponding to the inlets and the outlets, and the two are connected through bolts to clamp and fix the sample layer and the functional layer; the magnetic field source is arranged at the end where the sample outlet is located and on one side opposite to the functional layer, so that a gradient magnetic field which is gradually enhanced from the inlet to the outlet is formed in the flow channel.

A secretion analysis and sorting system where cell(s) are deposited into a carrier that will capture any secretions from the cell, and is small enough to be sorted using flow cytometry based cell sorting, is described herein. The use of an image classification workflow that identifies the event types present in a sample of carriers allows the user to select which event types they would like to purify, and then train a supervised classification system that will be used to make real time sort decisions to purify the carriers which contain cells that secrete the desired product. A flow cytometer is then able to be used to screen the individual carriers to see if each one has the desired secreted product.

The invention provides cell culture conditions for culturing stem cells, including feeder-free conditions for generating and culturing human induced pluripotent stem cells (iPSCs). More particularly, the invention provides a culture platform that allows long-term culture of pluripotent cells in a feeder-free environment; reprogramming of cells in a feeder-free environment; single-cell dissociation of pluripotent cells; cell sorting of pluripotent cells; maintenance of an undifferentiated status; improved efficiency of reprogramming; and generation of a naïve pluripotent cell.

The utility model provides a micro-fluidic chip for unmarked or fluorescently marked single cell sorting and a single cell sorting system, which do not adopt means for damaging a sorting target, such as high voltage, strong electric field, high-voltage sheath fluid and the like, and can be used for sorting the unmarked or fluorescently marked single cells on the premise of keeping activity. For unlabeled or labeled (such as fluorescence labeled) cells, bacteria, proteins, viruses, genes (fragments or whole segments), cell clusters and other microorganisms, or liquid drops, liquid segments, bubbles or blocky solids containing the biological particles, high-efficiency, accurate and reliable single-individual detection, control and sorting (especially including but not limited to single-cell sorting) are carried out.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The invention relates to the field of biological materials, in particular to a preparation method of magnetic microspheres and amino and / or carboxyl magnetic microspheres. The invention provides a preparation method of magnetic microspheres, which comprises the following steps: carrying out first polymerization reaction on styrene in the presence of an initiator to obtain polystyrene seed microspheres; swelling the seed microspheres in the presence of a pore-foaming agent, a monomer and an initiator to generate a second polymerization reaction; extracting and washing to obtain porous microspheres; modifying carboxyl on the inner and outer surfaces of the porous microspheres through a third polymerization reaction, mixing with iron ions, and coprecipitating to obtain the monodisperse superparamagnetic microspheres. The magnetic microspheres provided by the invention have monodispersity and superparamagnetism, and the particle size and the magnetic content of the magnetic microspheres can be regulated and controlled as required; the surface of the microsphere is rich in amino or carboxyl functional groups, and the content range of amino and carboxyl can be regulated and controlled according to the requirements of coupled protein; the method can be widely applied to the fields of in-vitro detection, biomedical research, protein separation and purification, cell sorting and the like.

The utility model relates to the technical field of cell sorting, and discloses an electrode assembly which comprises a positive electrode and a negative electrode, the positive electrode and the negative electrode are arranged on the same side of a liquid drop flow channel, and the positive electrode and the negative electrode form an electric field acting on a liquid outlet section of the liquid drop flow channel when powered on. The target liquid drops deflect to the side where the positive electrode and the negative electrode are located under the action of the electrophoretic force. By using the electrode assembly disclosed by the utility model, the flux of the separated liquid drops can be improved. The invention further discloses a micro-fluidic chip and a liquid drop sorting device.

The embodiment of the specification provides a preparation method of amination modified magnetic beads, and the preparation method comprises the following steps: mixing ferric salt, a ferrous salt aqueous solution and a dextran aqueous solution for reaction to obtain dextran magnetic beads; then adding an epichlorohydrin solution into the glucan magnetic beads to react for a certain time, and then adding an amination reagent to react for a certain time to obtain amination modified magnetic beads; the embodiment of the invention also provides a magnetic bead for cell sorting and cell activation, and a target cell sorting and target cell activation method. The cell sorting magnetic bead provided by the invention is high in cell sorting purity, high in sorting recovery rate and high in cell viability; the activation magnetic bead provided by the invention has a good activation effect, and activated cells can be amplified at high times.

The invention provides a cell magnetic sorting column and a preparation method thereof, and belongs to the technical field of cell magnetic sorting, the method comprises the following steps: S1, pretreating balls; s2, coating the surface of the ball with a thermoplastic high polymer material layer by adopting an electro-deposition process to form magnetic matrix particles; s3, sequentially putting a lower-layer filter screen, magnetic matrix particles and an upper-layer filter screen into the bottom shell of the separation column; s4, the upper sealing cover and the bottom shell are connected through ultrasonic welding; s5, centrifuging the sorting column subjected to ultrasonic welding; and S6, curing the centrifuged separation column. The cell magnetic separation column and the preparation method thereof are particularly suitable for magnetic separation of nanoscale magnetic beads, the problems that a traditional separation column is high in magnetic bead wastage rate, uneven in magnetic field, prone to blockage, prone to cell damage and the like can be solved, a high-purity and high-activity cell source is provided for cell therapy such as CAR-T, and the cell magnetic separation column is suitable for magnetic separation of nanoscale magnetic beads. And the process is simple and suitable for large-scale standardized production.

The invention belongs to the technical field of biology, particularly relates to a mouse CD19 monoclonal antibody, and further discloses a preparation method and application of the mouse CD19 monoclonal antibody. The heavy chain complementarity determining region of the CD19 monoclonal antibody comprises HCDR1, HCDR2 and HCDR3, and the light chain complementarity determining region of the CD19 monoclonal antibody comprises LCDR1, LCDR2 and LCDR3. The mouse CD19 specific antibody provided by the invention can specifically recognize and combine mouse CD19 protein and CD19 + cells, has high affinity, can be used for flow cytometry detection and cell sorting of mouse CD19 + cells, has high sensitivity and specificity, and has wide application prospects in detection and sorting of mouse CD19 protein and CD19 + cells.

The invention discloses a cell sorting micro-fluidic chip and application, the micro-fluidic chip is obliquely arranged on an inclined plane, the micro-fluidic chip comprises a substrate, the substrate is provided with a slender groove with a triangular prism profile, and a micro-fluidic channel is embedded in the slender groove; the upper end and the lower end of the inclined surface respectively face the center and the edge of the turntable; one side of the microfluidic channel back to the substrate is parallel to the upper surface of the substrate, one end of the microfluidic channel is communicated with the fluid buffer cavity, the other end is communicated with the sorting channel, and the other end of the sorting channel is communicated with the plurality of sorting sub-channels; a soft magnet is further arranged on the microfluidic channel, and a permanent magnet is arranged on one side, back on to the microfluidic channel, of the soft magnet. According to the invention, two-parameter sorting of preferential separation of magnetic microspheres according to density and accurate positioning according to size can be realized, high-flux and high-precision two-parameter sorting is realized, the resolution is greatly improved, the energy consumption is low, and the device is suitable for portable equipment.

The invention discloses a method for screening and identifying ribosome entry sites in a short chain, which comprises the following steps of: (1) screening a short chain IRES sequence with the length of less than 100nt from an IRES database, constructing a screening library and synthesizing; (2) constructing a screening vector containing double reporter genes, cloning the short-chain IRES into a linearized screening vector, and generating circular RNA which only depends on the short-chain IRES sequence to recruit ribosome expression of the reporter genes in cells by the screening vector cloned with the short-chain IRES sequence; and (3) carrying out cell transfection on the screening vector cloned with the short-chain IRES sequence, screening out cells expressing reporter genes through cell sorting, and sequencing through a ribosome-newborn peptide chain compound to obtain the short-chain IRES sequence capable of recruiting ribosome to start circular RNA translation in the cells. According to the invention, a short-chain IRES sequence library is constructed, and short IRES capable of starting circular RNA translation is screened, so that transfection limitation caused by overlarge molecular weight of the current IRES sequence is overcome.

The application provides a preparation method of a multi-layer sandwich type epoxy group modified magnetic microsphere and application thereof. The multi-layer sandwich type epoxy magnetic microsphere prepared by the preparation method provided in the application can realize efficient and accurate cell sorting through coupling of an antibody, is suitable for cell sorting related scenes such as stem cell separation, tumor cell detection, immune cell treatment (such as CAR-T cell preparation) and pathogenic microorganism enrichment, and provides key material support for cell sorting technology in life science research and clinical diagnosis and treatment.

In alternative embodiments, provided are self-activating Förster resonance energy transfer (saFRET) biosensors, and methods for making and using them. In alternative embodiments, provided are self-activating FRET (saFRET) biosensors, and methods that couple FRET and sequencing (FRET-Seq) to integrate random mutagenesis, fluorescence-activated cell sorting (FACS), and next-generation sequencing (NGS) to screen and identify sensitive biosensors from large-scale libraries directly in mammalian cells, utilizing the design of saFRET biosensors as provided herein.

The application relates to the technical field of cell sorting, and specifically provides a feature fusion method and system for high-throughput cell sorting. The method comprises the following steps: acquiring real-time image feature distribution deviation according to real-time image feature distribution and reference image feature distribution, and acquiring real-time spectral feature distribution deviation according to real-time spectral feature distribution and reference spectral feature distribution; calibrating all real-time image features according to the real-time image feature distribution deviation, and calibrating all real-time spectral features according to the real-time spectral feature distribution deviation; for each cell to be sorted, fusing real-time image features and real-time spectral features to obtain a fusion feature vector, and then classifying the cell to be sorted according to the fusion feature vector; the method can effectively improve the long-term stable operation capability, performance repeatability, reliability in standardized application scenarios and reduce the complexity and time consumption of system calibration and troubleshooting of a high-throughput cell sorting system.

The invention relates to the technical field of cell sorting, and particularly provides a feature fusion method and system for high-throughput cell sorting, and the method comprises the steps: obtaining a real-time image feature distribution deviation according to real-time image feature distribution and reference image feature distribution, acquiring a real-time spectral feature distribution deviation according to the real-time spectral feature distribution and the reference spectral feature distribution; calibrating all real-time image features according to the real-time image feature distribution deviation, and calibrating all real-time spectral features according to the real-time spectral feature distribution deviation; for each to-be-sorted cell, fusing the real-time image feature and the real-time spectral feature to obtain a fused feature vector, and then classifying the to-be-sorted cells according to the fused feature vector; according to the method, the long-term stable operation capability, the performance repeatability and the reliability in a standardized application scene of the high-throughput cell sorting system can be effectively improved, and the complexity and time consumption of system calibration and troubleshooting are reduced.

The invention discloses a portable multi-stage cell sorting device and a sorting method thereof, and belongs to the technical field of cell sorting. A pull plate and a rotating rod drive a deflection seat to rotate, so that the deflection seat pulls a sliding plate through a hinge rod, and the sliding plate drives a clamping strip to be separated from a clamping groove, so that a centrifugal rotor is conveniently detached; the centrifugal rotor is connected to the top of the butt joint base in a sleeving mode, after the clamping base is completely clamped into the rectangular groove, the deflection base is driven to rotate through the torsion effect of the torsion spring, the deflection base pushes the sliding plate to move through the hinge rod, the sliding plate drives the clamping strip to be clamped into the clamping groove, and the purpose of locking the centrifugal rotor and the butt joint base is achieved. A traditional mode of adopting thread locking or bolt fixing is abandoned, auxiliary calibration is carried out on the centrifugal rotor through the butt joint base, the central axis of the centrifugal rotor and the central axis of the butt joint base are concentric, the situation that dynamic balance is affected by deviation during installation is prevented, the centrifugal rotor is disassembled and assembled through a clamping structure, and operation convenience and maintenance efficiency are improved.

The application discloses a micro-fluidic chip for sorting sub-micron scale cells, which is composed of three components of an upper cover plate, a cell sorting micro-channel and a lower bottom plate by layer bonding. The upper cover plate is provided with an outside inlet, an inside inlet, an outside outlet and an inside outlet. The cell sorting micro-channel is composed of three regions of an inlet structure adjusting region, a spiral channel region and an outlet region. The lower bottom plate is provided with a convex wedge-shaped structure, and three kinds of inlet structures of an outside-narrow-inside-wide shape, an outside-inside-equivalent shape and an outside-wide-inside-narrow shape can be obtained by adjusting the position of the wedge-shaped structure. The sub-micron scale cells are sorted by the cooperation of the cell sorting micro-channel and the wedge-shaped structure. The spiral micro-channel is introduced, the cell transverse migration starting point is controlled by adjusting the spiral channel inlet structure and cooperating with suitable flow conditions, and the high-precision sorting of the sub-micron scale cells is finally completed. The cell sorting diversity and adaptability are improved by flexibly adjusting according to specific sorting requirements.