53 results about "Flow sorting" patented technology

Many areas of biomedical research depend on the analysis of uncommon variations in individual genes or transcripts. Here we describe a method that can quantify such variation at a scale and ease heretofore unattainable. Each DNA molecule in a collection of such molecules is converted into a single particle to which thousands of copies of DNA identical in sequence to the original are bound. This population of beads then corresponds to a one-to-one representation of the starting DNA molecules. Variation within the original population of DNA molecules can then be simply assessed by counting fluorescently-labeled particles via flow cytometry. Millions of individual DNA molecules can be assessed in this fashion with standard laboratory equipment. Moreover, specific variants can be isolated by flow sorting and employed for further experimentation. This approach can be used for the identification and quantification of rare mutations as well as to study variations in gene sequences or transcripts in specific populations or tissues.

A system and method for sorting of objects that includes a pathway network that has a plurality of pathways and one or more branch points. A fluid composition including one or more objects can be transported through the pathway network, where one or more of the objects are analyzed and sorted at one or more branch points based on the analysis of the objects.

The invention discloses a method for screening a CHO cell line high-expression site. The method comprises the following steps: integrating slow virus with a green fluorescence gene into a CHO cell genome, collecting monoclonal cells with high fluorescence expression quantity by a flow sorting method, performing expanding culture, and then screening out a corresponding high-expression integration site by using a chromosome walking technology. According to the method, a CRISPR/Cas9-mediated site-specific integration technology is combined, a to be expressed foreign gene can be quickly and accurately into a found site, and a foreign protein high-expression cell line can be quickly and efficiently obtained.

The present invention discloses a data packet recursion flow classification method, said method includes the following steps: pretreatment stage, dividing the fields contained by rule in rule base into several domains and defining number for stages for recursive classificaltion and combination condition of every domain; the domain according to rule is determined value or range, making respective treatment and producing pretreatment victor table of every stage for recursive classification; and search stage, searching the received data packet in pretreatment vector table or every stage, defining class identification belonging to the data packet, then respectively processing data packets according to the service types correspondent to the class identifications of data packets.

A method and apparatus for dynamically distributing traffic in a channel bonding wireless local area network (LAN) system. The method includes extracting information regarding a flow from an arrived packet and classifying the packet in the unit of the flow based on the extracted flow information; monitoring the state of each of bonded channels; and allocating the flow sorted in the extracting to the optimum channel by referring to information regarding the channel state monitored in the monitoring.

The invention discloses a dry-type separation and recovery system for circuit boards. The system comprises a crushing production line and a separating production line, wherein the crushing production line sequentially comprises a conveyer, a primary crusher, a secondary crusher and a tertiary crusher; the secondary crusher is arranged below the discharge hole of the primary crusher; the separating production line sequentially comprises a cyclone feeder, an air flow sorting machine, dedusting equipment and an electrostatic separator; the air flow sorting machine is arranged below the cyclone feeder. Compared with a conventional circuit board recovery method, the recovery process disclosed by the invention has the characteristics that no harmful substances are discharged to the environment, all wastes are recycled, and the energy consumption is low.

The invention discloses a desulfuration system for removing tar in desulfurization solution, comprising a desulfurizing tower, the desulfurization solution is pumped into a first-grade swirler from the desulfurizing tower, the top flow of the first-grade swirler is connected to a buffer tank, and the bottom flow of the first-grade swirler returns to the desulfurizing tower or is transported to a vacuum belt conveyor; the buffer tank is provided with an overflow position, and overflow flowing out of the overflow position returns to the desulfurizing tower; and seriflux in the buffer tank is pumped into a second-grade swirler, the top flow of the second-grade swirler is connected to a waste water tank, and the bottom flow of the second-grade swirler returns to the desulfurizing tower. In the invention, the desulfurization solution discharged from the desulfurizing tower by the system enters the first-grade swirler to be sorted; the top flow sorted by the first-grade swirler flows through the second-grade swirler to be sorted; finally the top flow of the second-grade swirler which contains most of tar is discharged from the desulfuration system to prevent the tar from affecting desulphurization efficiency, equipment service life and gypsum quality; and the separated waste water containing the tar is transported to a coal yard for humidifying coal and improving boiler steam generating efficiency.

A port monitor monitors network traffic that passes through a port of a switch. Frame flows routed through the switch are counted by hardware frame counting logic, which includes a content-addressable memory (CAM) and counters corresponding to various frame flows. Port monitor software includes logical containers (“buckets”), which are used to record and sort counts of the frame flows from hardware based on activity of the frame flows. Frame flow sorting is based on confidence building algorithms such that the thrashing of frame flows between buckets is reduced. Storage and sorting of the frame flows in software allows the activity level of any number of frame flows associated with the switch to be counted, regardless of hardware limitations on how many frame flows can be counted. This allows a real-time analysis of frame flows and a determination of frame flows that are major users of Fibre Channel bandwidth.

The present invention relates to a system and method of genomic profiling and is particularly useful in genomic differentiation of heterogeneous and polyclonal neoplastic cell populations, preferably of flow sorted formalin fixed paraffin embedded samples. The present invention includes methods of improving resolution for identifying aberration in variable carcinoma genomes and/or heterogeneous cell populations. The present invention also includes kits configured to improve genomic resolution and the ability to identify genomic aberration in variable and/or heterogeneous cell populations.

The invention discloses a breeding method for resistant super-male polyploidy asparagus based on flow cytometry. The method comprises the following steps: (1) choosing excellent male plants in a field and carrying out indoor planting and microscopic examination; (2) mechanically separating microspores; (3) carrying out flow sorting; (4) carrying out suspension culture; (5) screening resistant mutants; (6) carrying out artificial doubling; (7) carrying out flow analysis; (8) subjecting the microspores obtained in step (7) respectively to field selective matching and cross combination and to in vitro rapid propagation; and (9) carrying out variety comparative tests on plants obtained after field selective matching and cross combination and plants obtained after in vitro rapid propagation, and applying plants obtained in the tests in production in the field. The method provided by the invention has the following beneficial effects: (1) a breeding period is shorter, breeding efficiency is higher, and operation is simpler; (2) an obtained variety has comprehensiveness; and (3) a breeding technology is novel.

The invention relates to a method for analyzing a unicellular inclusion. The method comprises main steps of 1, by means of a portable single-particle pipettor, preparing a 96 or 384 orifice plate which loads a single coding microball; 2, sorting a unicell by means of a fluorescent activation flow sorting instrument, or separating a rare cell to a micro-orifice plate by means of the pipettor, thereby realizing quick one-to-one pairing between a unicell and a single microball; and 3, converting the inclusion information in the unicell to DNA sequence information by means of the coding microball,and analyzing the sequencing data by means of high-flux sequencing technology and bioinformatics. The method of the invention can realize high-efficiency stable low-cost separation of the single coding microball and has advantages of low technical threshold, effective overcoming of Poisson distribution, wide target range, controllable flux, low cost, etc. Furthermore the developed single-particlepipettor can be used for capturing and sequencing of the rare cell and can be widely used for the fields such as basic research and clinical diagnosis unicell analysis.

Described herein are methods related to generating intermediate mesoderm (IM) cells, including using sequential treatment of small molecules and growth factors, and composition produced by the described methods. Using small molecules such as CHIR99021 in combination with FGF2 and RA, efficient differentiation of human pluripotent stem cells (hPSCs) into intermediate mesoderm, such as PAX2+LHX1+ cells, is achieved. The method is extensible different hPSC cell lines and does not require flow sorting. Importantly, resulting PAX2+LHX1+ cells, are capable of WT1 expression and addition of FGF9 and activin, PAX2+LHX1+ cells specifically differentiates cells into SIX2, SALL1, and WT1 expressing cells representative of cap mesenchyme nephron progenitor cells. The described methods and compositions facilitate and improve the directed differentiation of hPSCs into cells of the kidney lineage for the purposes of bioengineering kidney tissue and iPS cell disease modeling.

The invention provides an application of miR-99a as an acute myelogenous leukemia stem cell molecular marker and also an application thereof in evaluation of prognosis, disease progression and treatment of the acute myelogenous leukemia. In the application, with flow sorting and real-time fluorescent quantitative PCR technologies as bases and bone marrow miR-99a of leukemia patients as detection objects, through comparison of the expression level between leukemia stem cell and leukemia non-stem cell and the expression level between leukemia stem cell and normal hemopoietic stem cell in the leukemia patients, abnormal high expression of the miR-99a in the leukemia stem cell in relapsed and stubborn patients is found and is related to bad prognosis of the patients, so that the miR-99a, as the marker and molecular target of the leukemia stem cells, is a new molecular marker for prognosis evaluation of the leukemia, and also provides the new molecular target for individual treatment. The application is used for preparing a diagnosis therapy product on the basis of detection on expression of the miR-99a, has important significance and clinical application value for tumor therapy and has wide application prospect.

The invention discloses a winnowing and color sorting method capable of preventing tea leaf damage. The method has the advantages that tea leaf flow sorting can be adjusted accurately by the joint action of a wind-driven feeding wheel and a first wind distribution plate capable of controlling wind quantity in dividedly, and the wind force of a second wind distribution plate can be adjusted according actual sorting needs so as to adjust the angle of the wind bending downwards during secondary winnowing; a tea leaf winnowing and color sorting machine uses a control unit for controlling the whole sorting process to allow the tea leaf flow inside the machine to be even and smooth, error discharging caused by sorting overload during winnowing can be prevented, and tea leaves with excellent quality enters a fermentation process.

The invention discloses a high-affinity human monoclonal antibody against yellow fever virus and application thereof, and belongs to the technical field of medicines. The high-affinity human monoclonal antibody against yellow fever virus is prepared by the following steps: taking E protein of yellow fever virus expressed in Escherichia coli as an antigen; screening memory B cells which can specifically bind to the E protein of yellow fever virus from PBMCs of a convalescent patient by performing flow sorting, thereby further obtaining variable-region fragments of an antibody; connecting the variable-region fragments of the antibody with a constant region to an expression vector; carrying out expression in mammalian cells, and carrying out purification; and then, carrying out a series of function tests, thereby obtaining 1 strain of the high-affinity human monoclonal antibody against yellow fever virus. The affinity between the antibody and the antigen is 1.49pm; moreover, the antibodyhas very strong yellow fever virus neutralization activity and IC50 at 4ng/ml. In addition, the antibody is capable of completely protecting mice from yellow fever virus at lethal dose. Thus, the high-affinity human monoclonal antibody against yellow fever virus has application values in clinical treatment and prevention of yellow fever virus.

The invention relates to a separation method of liver cancer stem cells. The separation method comprises the following steps: (1) culturing liver cancer cells by virtue of a DMEM high-glucose culture medium; (2) carrying out digestion passage on liver cancer cell culture fluid obtained in the step (1); and (3) marking passage cells being in a logarithmic phase during the digestion passage in the step (2) by virtue of flow sorting fluorescent antibodies CD90 and CD24, sorting by virtue of a flow cytometer, so as to obtain the separated liver cancer stem cells which are CD90+CD24+liver cancer cell subsets marked with CD90 and CD24. According to the separation method, two markers are utilized for carrying out joint detection, so that the separation and authentication successful rates of the liver cancer stem cells are increased; the operation is rapid, and the liver cancer stem cells can be rapidly and efficiently separated; the separation method is a new method for separating the liver cancer stem cells and has a good application prospect.

A disclosed method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell comprises the following steps: preparing cervical carcinoma cell, and performing in-vitro adherent culture on the cervical carcinoma cell; collecting cervical carcinoma cell at logarithmic phase, and adding the cell into Hoechst33342; sorting SP cells by using a superspeed flow sorting system, and applying SP cell and NSP cell obtained through sorting to further experiment research; and then adding cis-platinum with different concentrations respectively into NSP cell and SP cell, staining by using an Annexin V FITC cell apoptosis detection kit, performing detection by using a flow cytometer, performing different doses of radiotherapy on SP cell and NSP cell, staining by using an Annexin V FITC cell apoptosis detection kit, and performing detection by using a flow cytometer. The cell apoptosis rate is equal to UR% plus LR%. Cervical cancer stem cell screening is performed by taking SP cell as an entry point, chemoradiotherapy sensibility difference and possible mechanism are analyzed, and theoretical basis is provided for researching and developing tumor stem cell resisting medicines, some cytokines interdicting signal path and chemical substances.

The invention discloses a feature database generation method. The method comprises the following steps: extracting target flow corresponding to a target application; initially classifying the target flow under a preset dimension; extracting features from the classified target flow, and matching the extracted features with a saved history feature database in order to perform function prejudgment onthe target flow; generating an analysis tree specific to the target flow by taking each pre-judgment result as a branch; and generating a new feature database according to the analysis tree. The invention also provides a feature database generating device as well as a flow sorting method and device applying the feature database. Through adoption of the feature database generation method and device as well as the flow sorting method and device, the flow matching accuracy and efficiency can be optimized according to feature matching during flow sorting of the feature database.

The invention relates to a waste circuit board resource recycle and treatment system. The waste circuit board resource recycle and treatment system comprises crushing mechanisms, a first centrifugal fan, a first cyclone separator, an air flow sorting machine, a screening mechanism, a second centrifugal fan, a second cyclone separator and an adjustment and control valve; according to the waste circuit board resource recycle and treatment system, the multiple stages of crushing mechanisms are arranged, so that waste circuit boards can be fully crushed; the first centrifugal fan, the first cyclone separator, the air flow sorting machine, the screening mechanism, the second centrifugal fan, the second cyclone separator and the adjustment and control valve form a multi-stage sorting mechanism cooperatively, so that crushed materials output by the crushing mechanisms are fully sorted; the separation efficiency for the crushed materials is improved; manual crushing and sorting are not needed in the whole process; and the sorting efficiency is high.

The invention discloses a construction method and an application of an NK (natural killer) cell activation testing receptor signal path NKG2D fluorescence report system. A human NKG2D extracellular region gene sequence and mouse NKG2D transmembrane region and cytoplasmic region gene sequences are obtained by the aid of PCR (polymerase chain reaction) technology, the human and mouse NKG2D gene sequences are connected together by the aid of overlapping PCR technology, and the constructed human and mouse heterozygous NKG2D sequences are inserted into a pLXSN16E6E7 vector to form a vector pLXSN16E6E7-mhNKG2D. A human DAP12 gene sequence is constructed by the aid of the PCR technology and inserted into the pLXSN16E6E7 vector to form a vector pLXSN16E6E7-hDAP12. Plasmids of the two vectors and helper plasmids pCL-ECO of a retrovirus system are co-transfected with HEK293T cells to obtain virus particles containing reinfected mouse cells 3A9. Cell supernatants containing virus particles infect the cells 3A9, a cell line 12A9 steadily expressing mouse and human infusion NKG2D and human DAP12 is screened out by means of repeated flow sorting and re-culture, and downstream signals of NKG2D can be activated under the stimulus of human NKG2D ligand MICA/MICB, so that green fluorescence emitted by the cells can be detected by GFP (green fluorescent protein) expression.

The invention discloses a comprehensive utilization system of low rank coal. The system comprises a sieving device, a gas flow sorting device, a powdered coal steam treatment device, a mixing and kneading device, a molding device, a pyrolysis device, a first waste heat recycling device, a second waste heat recycling device and a coke tar oxidization device. The invention further provides a method for comprehensively utilizing the low rank coal by utilizing the system. By adopting the system and the method, disclosed by the invention, the problems that the powdered coal pelletizing rate is low, more semi-balls are formed, gas explosion is serious and the strength of molded balls is low are solved; the potential heat of pyrolysis gas is sufficiently utilized and energy sources are saved; meanwhile, stable and continuous operation of molding equipment and system is protected.