Method for analyzing unicellular inclusion

An inclusion, single-cell technology, applied in the field of single-cell sequencing, can solve the problems of high technical threshold, consumption, information deviation, etc., and achieve the effects of a wide range of analysis targets, high capture and release efficiency, and simple and convenient use.
CN109920482AInactive Publication Date: 2019-06-21XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2019-06-21
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention relates to a method for analyzing a unicellular inclusion. The method comprises main steps of 1, by means of a portable single-particle pipettor, preparing a 96 or 384 orifice plate which loads a single coding microball; 2, sorting a unicell by means of a fluorescent activation flow sorting instrument, or separating a rare cell to a micro-orifice plate by means of the pipettor, thereby realizing quick one-to-one pairing between a unicell and a single microball; and 3, converting the inclusion information in the unicell to DNA sequence information by means of the coding microball,and analyzing the sequencing data by means of high-flux sequencing technology and bioinformatics. The method of the invention can realize high-efficiency stable low-cost separation of the single coding microball and has advantages of low technical threshold, effective overcoming of Poisson distribution, wide target range, controllable flux, low cost, etc. Furthermore the developed single-particlepipettor can be used for capturing and sequencing of the rare cell and can be widely used for the fields such as basic research and clinical diagnosis unicell analysis.
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Description

technical field

[0001] The invention belongs to the field of single-cell sequencing, in particular to a method for analyzing single-cell content. Background technique

[0002] The heterogeneity of cells is a ubiquitous life phenomenon. As an independent living entity, the properties and differences displayed by a single cell play a vital role in the development of the entire living system. Every tissue and organ in the human body contains a variety of cell types, and each type of cell will change with the life activity of the organism. If thousands of single cells are studied, it will be blurred Therefore, it is extremely important to understand the working principles of complex organisms, the life functions and immune responses of each cell type to reveal the working mechanism of human tissues and organs and the laws of gene regulation. For example, malignant tumors that cause human cancer are highly heterogeneous tissues, composed of tumor cells of various phenotypes, and...

Claims

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